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121,623 results • Page 570 of 2433
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Novel Splice Variant Discovery from RNAseq Data
Variants Splice Computational RNAseq
3.9 years ago by saipra003 ▴ 20
11
votes
10
replies
2.8k
views
Searching for mRNA ending with a specific 3' pattern in NON-poly-A RNASeq data.
rnaseq poly-a
3.9 years ago by Pierre Lindenbaum 166k
4
votes
8
replies
2.8k
views
Forum: Troubleshooting Tips - bcl2fastq creates duplicate reads
bcl2fastq duplicates readgroup demultiplex
3.9 years ago by DavidStreid ▴ 110
3
votes
5
replies
1.6k
views
How to download protein sequences
plant fasta AQP
3.9 years ago by Nelo ▴ 20
5
votes
6
replies
2.2k
views
Align fastq SOLiD data
fastq aligner solid
3.9 years ago by Vic ▴ 130
0
votes
2
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1.1k
views
Regarding Error query in publically available RNA seq analysis by R studio
GEO Rstudio RNA-seq NGS
updated 19 months ago by 45k • written 3.9 years ago by • 0
6
votes
4
replies
10k
views
Can't locate Bio/SeqIO.pm - path error?
bioperl
updated 24 months ago by ▴ 180 • written 3.9 years ago by ▴ 280
4
votes
3
replies
6.4k
views
What is the correct syntax for BioPythons SeqIO.parse()
assembly biopython
updated 3.9 years ago by 8.8k • written 3.9 years ago by ▴ 110
0
votes
0
replies
664
views
What do the values ​​of max-allele frequency and min-allele frequency mean in the GATK GetPileupSumaries tool? (CalculateContamination)
getpileupsummaries calculatecontamination cross-contamination gatk
3.9 years ago by daniparada97 • 0
11
votes
11
replies
9.5k
views
7 follow
snpEFF not able to download GRCH38 ?
genome Annotation
3.7 years ago by pinn ▴ 210
1
vote
5
replies
2.7k
views
Kmergenie module readfq not found
readfq kmergenie
updated 2.2 years ago by 45k • written 3.9 years ago by ▴ 10
0
votes
0
replies
619
views
Functional Classification of Transcription Factor
factor database transcription
3.9 years ago by axolotl • 0
4
votes
6
replies
4.4k
views
How to output MDS plot for RNAseq samples with ggplot2?
RNAseq MDSplot ggplot2 R
updated 3.9 years ago by 88k • written 3.9 years ago by ▴ 80
1
vote
0
replies
964
views
Job: ELIXIR Technical Officer (bioinformatician/data scientist) at EMBL-EBI
standards EMBL-EBI data bioinformatician
3.9 years ago by Erin Haskell ▴ 470
0
votes
3
replies
1.5k
views
ChIP-seq peak number so high
chipsamples peaknumber chipseq chipseqpeaks chipseqanalysis
3.9 years ago by buffealo ▴ 130
0
votes
0
replies
1.6k
views
Job: Two PhD scholarships in Transporter Protein Engineering at DTU Biosustain
Yeast Engineering biology molecular Metabolic omic
3.9 years ago by info ▴ 80
0
votes
1
reply
780
views
Preprocessing exome sequence
Analysis workflow-Germline GATK Variant
updated 3.9 years ago by 88k • written 3.9 years ago by ▴ 70
0
votes
0
replies
909
views
News: Online Workshop - Bioinformatics Pipeline Development with Nextflow (November 22-25, 2021)
workshop pipeline NGS
3.9 years ago by David Langenberger 11k
2
votes
2
replies
1.5k
views
EM algorithm in Kallisto
RNA-seq Kallisto
3.9 years ago by Mozart ▴ 330
2
votes
2
replies
1.2k
views
Correct way to make multiple comparisons on DESeq2?
expression differential DESeq DESeq2 RNA-seq
3.9 years ago by jbnrodriguez ▴ 30
1
vote
0
replies
650
views
Where Can we download latest HGNC rdf file
owl python rdf sparql
updated 3.9 years ago by 56k • written 3.9 years ago by ▴ 10
0
votes
0
replies
630
views
HOW GISTIC do gene mapping
GISTIC
3.9 years ago by xingyu ▴ 10
1
vote
1
reply
1.3k
views
EMBOSS transeq lines with "(REVERSE SENSE)" in not reverse sence translations(?)
ORF transeq traduction EMBOSS
updated 3.9 years ago by ★ 29k • written 3.9 years ago by ▴ 20
2
votes
2
replies
1.6k
views
Highly mapped to introns
distribution read RSeQC RNA-seq exon intron
updated 3.9 years ago by 13k • written 3.9 years ago by ▴ 10
0
votes
2
replies
1.2k
views
Ways to analyze treatment changes in scRNAseq
cell scRNAseq RNA single sequencing
updated 3.9 years ago by 9.0k • written 3.9 years ago by • 0
0
votes
2
replies
1.2k
views
How to download fasta sequence of my interest from NCBI?
NCBI FASTA
updated 3.1 years ago by 45k • written 3.9 years ago by ▴ 170
1
vote
6
replies
1.9k
views
scRNASeq analysis Transcript abundance and gene abundance
abundance quantification scrnaseq transcript
3.9 years ago by SG ▴ 20
1
vote
4
replies
2.8k
views
Deseq2 - how to save zero counts on normalization step
deseq2 normalization
3.9 years ago by Sandhiya ▴ 20
1
vote
1
reply
1.4k
views
Job: Bioinformatics Scientist
scientist pharmaceuticals
updated 2.6 years ago by 45k • written 3.9 years ago by ▴ 10
1
vote
3
replies
3.4k
views
Samtools - [main_samview] fail to read the header
bam samtools
updated 3.9 years ago by 45k • written 3.9 years ago by ▴ 80
1
vote
1
reply
948
views
Refseq annotation for processed/unprocessed Pseudogenes
refseq pseudogene annotation genomics
updated 3.9 years ago by 152k • written 3.9 years ago by ▴ 60
9
votes
2
replies
5.5k
views
Clustering resolution for scRNA-seq
clustering single-cell
updated 3.9 years ago by ★ 1.7k • written 3.9 years ago by ▴ 10
0
votes
1
reply
605
views
promoter and insulator data download
promoter insulator data
updated 3 months ago by ▴ 90 • written 3.9 years ago by • 0
0
votes
0
replies
623
views
Single cell rna seq (scRNA Seq) analysis - TRANSCRIPT /GENE ABUNDANCE OR QUANTIFICATION
scRNA quantification gene transcript
3.9 years ago by SG ▴ 20
0
votes
4
replies
3.3k
views
Kraken2 output visualisation with Krona Viruses not shown
metagenomics kraken2 krona virus
3.8 years ago by maja.nackenhorst • 0
2
votes
3
replies
3.6k
views
selscan not outputting ihs
selscan haplotypes vcf
updated 3.9 years ago by ▴ 10 • written 6.2 years ago by ▴ 170
0
votes
0
replies
731
views
Rvtests SKAT-O output
rvtests sequencing SNP
3.9 years ago by @anna • 0
2
votes
0
replies
1.5k
views
Remove related samples using plink
plink ibd pihat plink2
3.9 years ago by sumudu_rangika ▴ 50
4
votes
3
replies
1.1k
views
Remove text and add a string in odd rows in a fasta file using awk
awk
updated 3.9 years ago by 21k • written 3.9 years ago by ▴ 30
5
votes
2
replies
1.2k
views
FFPE and fresh-frozen samples in different RNA seq batches?
batch RNA-seq fresh-frozen FFPE
3.9 years ago by Axel ▴ 10
1
vote
1
reply
842
views
Editing header of a fasta file
headers fasta
updated 3.9 years ago by 21k • written 3.9 years ago by ▴ 10
0
votes
2
replies
1.5k
views
How do I determine p-value and fold change for TMM normalized data?
TMM P-value normalization
updated 3.9 years ago by 88k • written 3.9 years ago by • 0
0
votes
3
replies
1.6k
views
Dragen-gatk pipeline information ?
germline gatk dragen wgs
updated 3.9 years ago by ★ 3.0k • written 3.9 years ago by ▴ 120
0
votes
0
replies
902
views
Error in merged bam files
picard GATK NGS
3.9 years ago by priya.bmg ▴ 70
0
votes
0
replies
516
views
What are the steps of an full lengh RNA seq annotation method?
genome-annotation
3.9 years ago by ilante ▴ 30
1
vote
11
replies
3.3k
views
desEQ2 eRROR
featurecount NA DESeq2 values
3.8 years ago by Nai ▴ 50
0
votes
0
replies
849
views
Herald: The Biostar Herald for Tuesday, September 14, 2021
herald
3.9 years ago by Biostar 3.6k
0
votes
0
replies
554
views
How to create phylogenic tree from NCBI taxon ID-s
tree phylogenic R
3.9 years ago by Roland • 0
0
votes
2
replies
1.2k
views
What is the difference between filtering cells before creating Seurat object and after that?
Seurat filter cells
3.9 years ago by re_raz ▴ 90
0
votes
2
replies
951
views
Genome mapping and annotation
annotation genome
3.9 years ago by claudia • 0
121,623 results • Page 570 of 2433
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Problem with .bed file
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Comment: New to Bioinformatics, Phage genome investigation
Comment: Feedback needed on new user-friendly BED file tool
C: BRIG: How to use a multi fasta file as query
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Comment: Intro + Question by akshitabadrinath • 0
Thanks @yokofakun for your reply. Can contributions be in the form of documentation to the code base?
Comment: Intro + Question by Pierre Lindenbaum 166k
https://github.com/google/deepvariant/blob/r1.9/CONTRIBUTING.md > We cannot merge external pull requests into the DeepVariant repository …
Comment: New to Bioinformatics, Phage genome investigation by Michael 56k
Ok, thank you for the explanation. As I said, I am no expert in phage biology. > reading a ton of research papers and collecting specific…
Comment: New to Bioinformatics, Phage genome investigation by Hanhan2000 • 0
Strictly lytic phages will not have integrate or excisionase, as those are only required for the dormant lysogenic cycle. Finding their pre…
Comment: Feedback needed on new user-friendly BED file tool by GenoMax 152k
While new tools are always welcome (specially if they are easier to use for newcomers) you provide no links for the tool so people can try …
Comment: New to Bioinformatics, Phage genome investigation by Michael 56k
Finding genes by similarity in a few small genomes is an easy task in bioinformatics, but you must make sure that you are asking the right …
Comment: Issues for Purchasing The Biostar Handbook – Professional Edition by GenoMax 152k
You can email admin at biostars dot org with this question. Also ask your friend to check with their bank that their credit card is valid…
Comment: Download Isoform expression data from tcga by t.fosk • 0
found some data here in case someone needs them in the future: https://xenabrowser.net/datapages/?cohort=TCGA%20Pan-Cancer%20(PANCAN)&rem…
Comment: Does edgeR supports random effects? by 406339165 ▴ 10
Hi all, Thank you for the helpful suggestions. In my dataset, I observed a significant effect of Sex, so I subset the data to include only…
Comment: Looking for neanderthal genomes to download by RD ▴ 20
Hey @severalorks, Were you able to figure it out? I'm curious how you built the consensus sequences and aligned them to human proteins. I n…
Comment: How to pull out protein sequences from from BAM files containing genomic alignme by RD ▴ 20
Hey @Julia92796, Were you able to figure it out? I'm curious how you built the consensus sequences and aligned them to human proteins. I no…
Comment: STAR alignment to small fasta file failing by GenoMax 152k
What is the length of fasta reference sequences?
Comment: Significance of the term "tile" in sequencing? by GenoMax 152k
What kind of sequencer is this data from?
Comment: STAR aligned BAM files by Ram 45k
> but it seems that i cannot directly use these BAM files in STAR. You most definitely can.
Answer: STAR aligned BAM files by Ram 45k
You cannot use STAR to get isoform level counts, only gene level counts and you can do that using an input BAM. For transcript level counts…
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