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121,912 results • Page
58 of 2439
Sort: Rank
Rank
Views
Votes
Replies
0
votes
0
replies
383
views
Why does the normal tissue increase while I try to select the tumor purity great than 60%
Tumor-purity
TCGA
updated 10 months ago by
Ram
45k • written 10 months ago by
nuorain
▴ 40
0
votes
4
replies
1.5k
views
Seurat - unordered cells
R
Seurat
10 months ago by
solo.albif
• 0
0
votes
1
reply
621
views
GISTIC 2.0 parameter change not happening
CNV
GISTIC
updated 10 months ago by
Ram
45k • written 10 months ago by
Swattik
• 0
3
votes
1
reply
706
views
How to find the adapter sequence of an RNAseq dataset?
fastQC
trimming
adapter
RNAseq
updated 10 months ago by
GenoMax
153k • written 10 months ago by
Maryam
• 0
2
votes
4
replies
1.0k
views
Compare Two Sigle-cell Sample for Differentially Expressed Genes (DEGs). How?
single-cell
updated 10 months ago by
Ram
45k • written 10 months ago by
wangziwei0010
▴ 30
0
votes
2
replies
652
views
Repeat CT in overrepresented sequences
ScRNA
fastqc
overrepresented
sequences
updated 10 months ago by
allingt
▴ 40 • written 10 months ago by
Analeigh
• 0
1
vote
1
reply
623
views
Can a read be aligned more than once?
alignment
gaf
read
sam
updated 10 months ago by
GenoMax
153k • written 10 months ago by
guntul
▴ 40
1
vote
6
replies
1.6k
views
What is a genomic range that would almost always get decent WGS coverage but is never covered by WES?
wes
wgs
updated 10 months ago by
i.sudbery
22k • written 10 months ago by
Jeremy Leipzig
23k
1
vote
1
reply
802
views
Using NumReads column of quants.genes.sf file to build DESeq2 input
R
deseq2
rna-seq
salmon
updated 10 months ago by
i.sudbery
22k • written 10 months ago by
Ezequiel
▴ 10
1
vote
1
reply
553
views
SCE object acting strangley
R
SCE
Seurat
SingleCellExperiment
updated 10 months ago by
Ram
45k • written 10 months ago by
wykpenguin
• 0
0
votes
5
replies
1.1k
views
why I get "Running twoBitToFa failed" when loading chopchop
chopchop
twoBitToFa
CRISPR
updated 5 months ago by
GenoMax
153k • written 10 months ago by
WYZ1120zzh
• 0
0
votes
0
replies
583
views
TCGA differential DNA methylation analysis 450K best practices
methylation
HM450K
differential
10 months ago by
Brassicka
• 0
0
votes
3
replies
797
views
Conversion from .lib to .faa file
FASTA
BLAST
updated 10 months ago by
Ram
45k • written 10 months ago by
watermicrogirl
• 0
0
votes
2
replies
793
views
Inquiry about depth of coverage and copy number variation
number
copy
CNV
sequencing
depth
variation
10 months ago by
Chen
• 0
1
vote
1
reply
551
views
Finding Differential Phosphosites in TMT Phosphoproteomics Data
DEqMS
Mass
Phosphoproteomics
Proteomics
Spec
updated 10 months ago by
ATpoint
89k • written 10 months ago by
jesswhitts
• 0
0
votes
1
reply
603
views
Magnesium ion in a post-docking adjustment
Docking
Magnesium
Ion
updated 10 months ago by
dthorbur
★ 3.1k • written 10 months ago by
Rodolfo Adrián
• 0
1
vote
4
replies
1.3k
views
R encountering fatal error and quitting the session
R
randomForestSRC
Random-forest
updated 10 months ago by
Dunois
★ 2.9k • written 10 months ago by
anjalivasudevan98
▴ 40
3
votes
1
reply
773
views
scRNA seq dissimilarity plot
anosim
scRNA-seq
dissimilarity
updated 10 months ago by
Bastien Hervé
6.5k • written 11 months ago by
carolofharvest
▴ 50
1
vote
6
replies
1.6k
views
dittoHeatmap; adding column labels on the heatmap
pheatmap
ComplexHeatmap
dittoHeatmap
updated 10 months ago by
jared.andrews07
★ 19k • written 10 months ago by
Varun Gupta
★ 1.3k
3
votes
3
replies
962
views
IDAT to FASTA workflow
PLINK
R
IDAT
FASTA
GWAS
updated 10 months ago by
GenoMax
153k • written 10 months ago by
sabrilo171
▴ 20
2
votes
7
replies
1.7k
views
GATK - Output differences in Calls when Split by chromosome intervals and without splitting
GATK
PICARD
MergeVcf
vcftools
10 months ago by
Maverick
▴ 10
0
votes
0
replies
541
views
mageck mle design matrix
crispr
mageck-mle
design-matrix
pooled-screen
10 months ago by
Assa Yeroslaviz
★ 1.9k
1
vote
2
replies
1.2k
views
After subsetting a seurat object number of variable features is wrong.
FindVariableFeatures
seurat
subset
updated 10 months ago by
Ram
45k • written 13 months ago by
Assa Yeroslaviz
★ 1.9k
0
votes
1
reply
726
views
PyDeseq2 - InvalidIndexError
python
rna-seq
pandas
pydeseq2
deseq2
updated 10 months ago by
Dunois
★ 2.9k • written 10 months ago by
tmanjonas
• 0
0
votes
0
replies
447
views
Finding the IC50 from GraphPad Prism
IC50
10 months ago by
camiinnocente
• 0
0
votes
0
replies
349
views
Pipelines for CAZenzymes, COG, KO, MetaCyc, Pfam and TIGRFAM analysis
PICRUSt2
Metagenomic
MicFunPred
updated 10 months ago by
Ram
45k • written 10 months ago by
Petrônio
• 0
0
votes
1
reply
687
views
Gene performance/coverage calculation methodology in .bam files using samtools
samtools
ngs
coverage
updated 10 months ago by
Ram
45k • written 10 months ago by
slzr_
▴ 10
1
vote
3
replies
1.8k
views
Error in CIBERSORTx ($ operator is invalid for atomic vectors)
RNA-Seq
CIBERSORTx
deconvolution
cibersort
updated 10 months ago by
jurriaanjanssen
• 0 • written 19 months ago by
star
▴ 10
1
vote
12
replies
8.3k
views
7 follow
Error in CIBERSORTx
RNA-seq
CIBERSORT
LM22
updated 10 months ago by
jurriaanjanssen
• 0 • written 2.9 years ago by
Manav
• 0
9
votes
5
replies
27k
views
PC3 & PC2 using plotPCA function in R
R
plotPCA
PCA
updated 10 months ago by
Ram
45k • written 8.5 years ago by
User000
▴ 750
0
votes
0
replies
407
views
(vg rna) some transcripts have multiple projections on haplotypes and abnormal length growth
vg
10 months ago by
Wenhai
• 0
0
votes
1
reply
716
views
Minor Allele Frequency Calculation for 23andMe data
MAF
GWAS
PGS
updated 10 months ago by
barslmn
★ 2.4k • written 10 months ago by
Priyatam
• 0
1
vote
2
replies
755
views
De novo assembly with more genome size than expected
illumina
sequencing
assembly
genomics
updated 10 months ago by
GenoMax
153k • written 10 months ago by
Isidro
• 0
1
vote
2
replies
1.1k
views
Why use Hi-C and/or Bionano for scaffolding when Nanopore sequencing exists?
wgs
hic
sequencing
nanopore
genomics
updated 10 months ago by
shelkmike
★ 1.8k • written 10 months ago by
Mark
▴ 60
3
votes
7
replies
2.0k
views
HTSeq-count vs. FeatureCounts: Significant Difference in Results
FeatureCounts
HTseq-count
10 months ago by
Dora
▴ 10
1
vote
0
replies
363
views
Enironmental metabolic ability - metagenomic assembly or MAGs
MAGs
environmental
microbiology
metagenomes
updated 10 months ago by
GenoMax
153k • written 10 months ago by
sipeskm
▴ 10
12
votes
5
replies
2.7k
views
Tutorial:
removeBatchEffect explained using base R linear models
removebatcheffects
limma
batch-effect
updated 10 months ago by
SamGG
▴ 150 • written 16 months ago by
nhaus
▴ 420
2
votes
6
replies
3.0k
views
Question: can not obtain DESeq2 results
RNA-Seq
DESeq2
updated 10 months ago by
Jack
▴ 10 • written 5.2 years ago by
zjxia
▴ 10
1
vote
0
replies
312
views
Stable coordinates in gaf files?
gfa
gaf
rgfa
10 months ago by
Uveyik
▴ 80
1
vote
2
replies
839
views
Dealing with outliers for differential expression in proteomics
proteomics
PCA
DEA
outliers
limma
updated 10 months ago by
ATpoint
89k • written 10 months ago by
Jorge HB
• 0
0
votes
1
reply
529
views
Differential expression genes(downstream analysis)
Bulk-RNAseq
updated 10 months ago by
pinheirofabiano
▴ 130 • written 10 months ago by
odi
▴ 10
0
votes
1
reply
633
views
RNAhybrid + svm_light for miRNA target prediction
microrna
svm
target
rnahybrid
updated 10 months ago by
bingyanzheng18
• 0 • written 14 months ago by
Lucrezia
• 0
0
votes
0
replies
342
views
How do I set the RNAhybrid parameters to get more accurate and as many results as possible
RNAhybrid
miRNA_target_genes_prediction
10 months ago by
bingyanzheng18
• 0
1
vote
2
replies
624
views
Trinity analysis error
Trinity
10 months ago by
ongchip
• 0
0
votes
0
replies
454
views
CIBERSORTx error (error in rep(2, size * length(cells) -1)) : invalid 'times' argument
CIBERSORTx
CIBERSORT
scRNA-seq
RNA-seq
Deconvolution
10 months ago by
Amairani
• 0
2
votes
2
replies
788
views
Using RepeatModeller and RepeatMasker for Multiple Gastropod Genomes: Is a Single Repeat Library Sufficient?
genome-annotation
RepeatModeller
RepeatMasker
updated 10 months ago by
Mensur Dlakic
★ 30k • written 10 months ago by
Rohan
▴ 40
0
votes
2
replies
669
views
Reconciling old crystallography papers with modern sequencing data
PDB
proteomics
updated 10 months ago by
Wayne
★ 2.1k • written 10 months ago by
John Ma
▴ 310
0
votes
0
replies
321
views
Evaluate homopolymeric tracts in a set of microbial genomes
genomes
genomics
updated 10 months ago by
Ram
45k • written 10 months ago by
Madde
▴ 20
0
votes
5
replies
850
views
Coverage/depth of merged bam files
samtools
updated 10 months ago by
GenoMax
153k • written 10 months ago by
slzr_
▴ 10
0
votes
1
reply
523
views
Multiple Synteny Plot using MCScanX.
Synteny
MCScanX
updated 10 months ago by
alex.zaccaron
▴ 480 • written 10 months ago by
a.bibek52
▴ 10
121,912 results • Page
58 of 2439
Recent Votes
Getting VCF file for the 1000 genome project with rsid
Answer: Different UMAP for batch correction in R and Pytho
Answer: Different UMAP for batch correction in R and Pytho
Answer: Different UMAP for batch correction in R and Pytho
A: How to extract reads with no INDEL?
Answer: Different UMAP for batch correction in R and Pytho
Answer: WGCNA package in R: dendogram plot error
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Comment: Tried building a compact sequence format with 4-bit storage
by
Alex Reynolds
36k
In addition to looking at other tools as a baseline: https://en.wikipedia.org/wiki/Compression_of_genomic_sequencing_data maybe also look i…
Comment: Different UMAP for batch correction in R and Pytho
by
Madiha
▴ 10
Thanks, António for a helpful, detailed response. I didn't take into consideration that the plotting order of cells from each batch can be …
Comment: [vg giraffe] Unable to add read group to BAM file
by
saruman
• 0
It seems that using the following format fixes the problem: --read-group "1 SM:HG002 LB:lib1 PL:illumina PU:unit1" As shown below: …
Comment: Developing a genomics ML model using bytes?
by
dariober
15k
> what if we can use the bytes to develop a model? My understanding is that the OP is considering building a model using the sequences in …
Comment: WGCNA package in R: dendogram plot error
by
RJ
• 0
You might had delete the outliers from the expression table but then the traits data.frame doesn't match with expression table dimensions.
Comment: miRNA low mapping ratings
by
Ant
▴ 50
No, it's a personal dataset. I haven’t seen the link, but if I understand correctly, it's not possible to use the software for free, right?
Comment: miRNA low mapping ratings
by
GenoMax
153k
So this is a public dataset? QIAseq miRNA libraries may require special handling. Have you seen --> https://resources.qiagenbioinformatics.…
Comment: Trouble when installing arb
by
GenoMax
153k
Are you referring to software found here --> http://download.arb-home.de/release/latest/ ? If so why are you installing the source. Authors…
Comment: Hifiasm is getting killed while trying to assemble a genome
by
GenoMax
153k
All valid points. This is a SRA dataset. If it is covered by a publication hopefully it is not a bad one. @Pranav if you can post the SRA a…
Answer: Different UMAP for batch correction in R and Pytho
by
antonioggsousa
3.4k
Hi, I'm mostly an R user, but the codes you presented look correct to me. The two UMAP projections *"look too similar to be different"*, …
Answer: How to properly assemble phage genomes?
by
Joe
22k
This might help: https://millardlab.org/lab-members/alumni/lucy-gannon/lucys-beginner-guide-to-bacteriophage-genome-assembly/
Comment: RNA-SEQ where only a subset of genes is of interest
by
piffelpaff
• 0
Thanks for your reply. Yes it's single-cell and we're talking about roughly 30 pre-selected genes. I see. I was rather referring to the d…
Answer: Best set of tools / workflows for predicting the biosynthesis pathways of comple
by
Joe
22k
This may not be exactly what you're looking for (kind of the other way around, predicting synthetic clusters from genes rather than the pro…
Comment: Developing a genomics ML model using bytes?
by
ATpoint
89k
How is this different from https://www.biostars.org/p/9615062/
Comment: Shift in RNA 3' position, Term-Seq vs RNASeq
by
npb27
▴ 20
Hi thanks for your answer Interesting idea about ribosome length, although I can't think why this should affect the apparent 3' position b…
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