4.2 years ago by
joker33
▴
150
0
votes
0
replies
1.0k
views
0
votes
0
replies
897
views
0
votes
0
replies
823
views
2
votes
1
reply
1.4k
views
updated 4.2 years ago by
Pierre Lindenbaum
166k
•
written 4.2 years ago by
gevikoj888
•
0
0
votes
2
replies
11k
views
updated 3.2 years ago by
Ram
45k
•
written 9.9 years ago by
bioinforesearchquestions
▴
370
1
vote
2
replies
3.3k
views
0
votes
0
replies
1.1k
views
4.2 years ago by
kacollier
▴
30
0
votes
0
replies
817
views
1
vote
2
replies
1.3k
views
0
votes
0
replies
827
views
0
votes
0
replies
2.0k
views
4.2 years ago by
Shicheng Guo
★
9.6k
5
votes
5
replies
2.7k
views
2.7 years ago by
v.berriosfarias
▴
140
0
votes
0
replies
630
views
5
votes
6
replies
3.2k
views
1
vote
4
replies
1.4k
views
updated 2.6 years ago by
Ram
45k
•
written 4.2 years ago by
ConfusedSheep
▴
10
3
votes
3
replies
2.0k
views
1
vote
1
reply
914
views
5
votes
4
replies
3.3k
views
4.2 years ago by
charlielonergan
▴
20
0
votes
3
replies
1.3k
views
updated 4.2 years ago by
andres.firrincieli
3.9k
•
written 4.2 years ago by
bioAddict
•
0
0
votes
0
replies
782
views
0
votes
0
replies
692
views
1
vote
0
replies
670
views
0
votes
0
replies
1.6k
views
1
vote
0
replies
2.1k
views
6
votes
6
replies
2.8k
views
4.0 years ago by
gubrins
▴
350
1
vote
3
replies
1.3k
views
3
votes
4
replies
1.9k
views
4.2 years ago by
raalsuwaidi
▴
110
2
votes
3
replies
1.5k
views
5
votes
3
replies
13k
views
updated 4.2 years ago by
Nai
▴
50
•
written 5.6 years ago by
Balatheskulter
•
0
0
votes
1
reply
881
views
4.2 years ago by
geneticatt
▴
140
1
vote
4
replies
1.6k
views
4.2 years ago by
quentin54520
▴
120
1
vote
3
replies
1.8k
views
updated 4.2 years ago by
Pierre Lindenbaum
166k
•
written 4.2 years ago by
aka
▴
10
17
votes
11
replies
14k
views
8 follow
4
votes
6
replies
2.0k
views
4.2 years ago by
Evan
▴
250
0
votes
0
replies
721
views
4.2 years ago by
Hannah
•
0
0
votes
0
replies
1.0k
views
4.2 years ago by
Gregor Rot
▴
550
4
votes
6
replies
4.9k
views
0
votes
2
replies
1.2k
views
0
votes
0
replies
708
views
0
votes
0
replies
695
views
2
votes
2
replies
1.1k
views
updated 4.2 years ago by
Zhilong Jia
★
2.2k
•
written 4.3 years ago by
Shabnam.yazdanpanah1998
•
0
0
votes
0
replies
624
views
0
votes
1
reply
849
views
9
votes
6
replies
2.3k
views
3
votes
7
replies
12k
views
4.2 years ago by
arshad1292
▴
110
0
votes
6
replies
2.4k
views
4.2 years ago by
smrutimayipanda
▴
20
0
votes
0
replies
717
views
0
votes
2
replies
947
views
0
votes
0
replies
589
views
2
votes
5
replies
3.0k
views
Recent Votes
Recent Locations •
All
Durham, NC, United States, 5 minutes ago
Chile, 7 minutes ago
Mexico, 7 minutes ago
United States, 22 minutes ago
China, 40 minutes ago
London, UK, 41 minutes ago
United States, 41 minutes ago
Recent Awards •
All
Popular Question to
oliverhooker
▴
110
Popular Question to
pinheirofabiano
▴
130
Popular Question to
carolofharvest
▴
50
Teacher to
lieven.sterck
16k
Popular Question to
Physalia-courses
★
2.6k
Popular Question to
anon
•
0
Recent Replies
Comment: DESeq2 on metagenome KO counts
by
Aleksandra
▴
160
I apologise for how that sounded; I wanted to be as detailed as possible.
Comment: GUI commercial software for 10x single cell gene expression analysis
by
Istvan Albert
103k
A comment on: "*Wonky analysis is possible even with open-source R libraries.*"
True - and arguably, the risk of a wonky analysis is actua…
Comment: GUI commercial software for 10x single cell gene expression analysis
by
GenoMax
154k
`Trailmaker` likely only supports Parse's evercode data. <br>
`BioTuring` does not appear to have published pricing. <br>
`Rosalind` appear…
Answer: GUI commercial software for 10x single cell gene expression analysis
by
firestar
★
1.7k
Found a few more when I asked around. I have no idea how good or bad these are. If anyone has used these or knows more about these, feel fr…
Comment: How to handle TrEMBL proteins without gene annotation in plasma proteomics?
by
GenoMax
154k
> I performed a standard proteomics screening workflow
What database did you use to search against? Perhaps you are using an old (not so c…
Answer: FACS quality control based on size and doublet detection in scRNA-seq
by
ATpoint
89k
No, it's unrelated. Doublets in 10x happen if two cells get enclosed into the same GEM droplet. This has nothing to do with how flow cytome…
Answer: How to handle TrEMBL proteins without gene annotation in plasma proteomics?
by
Elisabeth Gasteiger
★
2.4k
Apparently you are working with human proteins? I would strongly recommend looking only at proteins that are part of the human reference pr…
Comment: Tools to simulate Illumina short read sequences and ONT long reads with a refere
by
colindaven
8.0k
Wow, thats too long. How much RAM does your machine have, it is likely swapping
Comment: How to handle TrEMBL proteins without gene annotation in plasma proteomics?
by
Elisabeth Gasteiger
★
2.4k
Note that UniProtKB accession numbers do not have any meaning. While most ACs starting with A0A5* are indeed unreviewed (i.e. from TrEMBL),…
Comment: DESeq2 on metagenome KO counts
by
young_bioinformatician
▴
240
Thank you but sorry, your answer sounds very AI-based...
Answer: How to handle TrEMBL proteins without gene annotation in plasma proteomics?
by
Aleksandra
▴
160
For basic analysis, discard anything that isn't from Swiss-Prot. Your statistics and pathway analysis should only be based on reliable, ann…
Answer: Recommendations for 200 SNP markers genotyping
by
Aleksandra
▴
160
For a 200-SNP panel at your sample throughput, I would recommend an amplicon-based targeted sequencing approach.
The workflow involves des…
Comment: Reasonable number of SNPs in a bacterial genome.
by
dthorbur
★
3.1k
There are *a lot* of factors that can impact the number of identified SNPs. These include things like:
- Evolutionary distance from samp…
Answer: DESeq2 on metagenome KO counts
by
Aleksandra
▴
160
Your proposed workflow is indeed sound, as applying DESeq2 to KO-counts is perfectly valid; its underlying negative binomial model is metho…
Comment: Interpreting genomic features distribution for CUT&RUN peaks
by
Rozita
▴
40
The massive difference has been reproducible across the 3 biological replicates and is in line with what we would expect in terms of the bi…
Traffic: 3571 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6