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Wow, I was shocked at this AI answer that looks to be a good start for using UniProt. Sharing it in case it is useful. (It will work in ses…
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> Can you clarify what you mean by "100% of reads pass cutadapt, even
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You didn't mention what kind of organism you are working with and what kind of protein you are looking for?
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Thank you Gordon for your expertise. One question that I have now that I have a list of DE genes is how to interpret logFC of this continuo…
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Yes I plan to use QIIME2, but I'm Cutadapting outside QIIME so its easier to MultiQC without importing/unzipping QZAs (and QIIME2 importing…
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Following your advice, I checked the primer and adapters sequences and edited the question accordingly
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We can't phase them out per se, because ``kallisto index`` is still the engine responsible for creating the index under the hood (and there…
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Also try the NCBI AWS bucket transfer tool. It's extremely convenient, however it's not instantaneous and you need to have a (paid) AWS acc…
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Check a random sampling of reads to make sure the adapters are being removed. You may also want to blast a few reads to ensure that there i…
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Many thanks for your comment. If read-through is happening, shouldn't Cutadapt being taking care of that there? I provided to the Cutadapt …
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Thank you. I will read the documentation you attached. Are you going to slowly phase out the not kb ref commands? Also, does the t2g.txt fi…
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