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65
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57
replies
8.4k
views
12 follow
Forum: Bioinformatics Mobile App
android genome iphone mutations
updated 2.3 years ago by 43k • written 9.5 years ago by ▴ 20
28
votes
14
replies
8.4k
views
8 follow
Forum: Should I work first in a Bioinformatics company or get a Ph. D.?
Biochemistry PhD career
updated 13 months ago by 43k • written 8.0 years ago by ▴ 20
60
votes
28
replies
8.3k
views
27 follow
Forum: Jim Robinson of the Integrative Genomics Viewer (IGV) uses this
uses-this
updated 14 months ago by 43k • written 9.6 years ago by 100k
4
votes
6
replies
8.3k
views
Forum: TCGA: How to tell if the data is exome or WGS?
wgs mutations tcga exome
updated 19 months ago by 43k • written 8.7 years ago by ▴ 110
129
votes
38
replies
8.3k
views
21 follow
Forum: Who are the Greatest Bioinformaticians Of All Time (GBOAT)
bioinformatics
updated 11 months ago by 43k • written 5.1 years ago by 100k
64
votes
8
replies
8.3k
views
7 follow
Forum: Isaac Asimov Accurately Predicts Woes Of Bioinformatician
bioinformatician
updated 14 months ago by 43k • written 11.0 years ago by 20k
116
votes
35
replies
8.3k
views
18 follow
Forum: Is Biostar Killing The Bioinformatics Core?
core biostars
updated 15 months ago by 43k • written 11.1 years ago by 22k
19
votes
9
replies
8.2k
views
6 follow
Forum: How to support bioinformatics tools via Biostars: prefill and watch tags, reply via email
biostars
updated 15 months ago by 43k • written 10.0 years ago by 100k
3
votes
5
replies
8.2k
views
Forum: PopGenome nucleotide diversity
next-gen SNP R gene
updated 13 months ago by 43k • written 8.2 years ago by ▴ 10
36
votes
18
replies
8.2k
views
Forum: How Does Genomebrowse Free Genome Browser From Golden Helix Compare?
visualization genome-browser
updated 3.1 years ago by 20 • written 11.1 years ago by ▴ 140
16
votes
6
replies
8.2k
views
7 follow
Forum: What computer skills should I put on my resume?
resume genome
updated 12 months ago by 43k • written 6.4 years ago by ▴ 30
27
votes
0
replies
8.1k
views
Forum: Heng Li of BWA and Samtools uses this
uses-this
updated 14 months ago by 43k • written 9.6 years ago by 100k
61
votes
16
replies
8.1k
views
14 follow
Forum: Rust or C++, what to learn after Go for high-performance bioinformatics tools?
library
updated 16 months ago by ★ 1.3k • written 3.6 years ago by 8.5k
15
votes
9
replies
8.1k
views
9 follow
Forum: Skills & qualities required to be a Bioinformatician
career skills
updated 23 months ago by 43k • written 7.1 years ago by ▴ 70
8
votes
12
replies
8.1k
views
9 follow
Forum: Mac Osx Confusing Vcf With Vcard
vcf
updated 14 months ago by 43k • written 11.1 years ago by ▴ 80
68
votes
22
replies
8.0k
views
16 follow
Forum: Learning Python: list of resources
python
updated 13 months ago by 43k • written 7.6 years ago by ★ 2.8k
34
votes
19
replies
7.9k
views
12 follow
Forum: Programming Language In Bioinformatics
perl python
updated 14 months ago by 43k • written 11.0 years ago by ▴ 260
5
votes
4
replies
7.9k
views
Forum: Masters In Bioinformatics Vs Masters In Information Technology (Computer Science)
career
updated 14 months ago by 43k • written 10.1 years ago by • 0
29
votes
4
replies
7.9k
views
Forum: Update to tbb (2021.1.1, conda-forge) breaks bowtie2 (bioconda)
software-error bowtie2 bioconda conda tbb
updated 14 months ago by 43k • written 3.2 years ago by ▴ 240
15
votes
6
replies
7.8k
views
Forum: Calling Single-Barcode Samples from Mixed Runs as Dual-Barcode Samples | Possible Illumina Run QC Flags?
Illumina De-Multiplexing barcode quality-scores
updated 15 months ago by 43k • written 5.1 years ago by 8.2k
42
votes
15
replies
7.8k
views
8 follow
Forum: Directions In Bioinformatics And Web-Based Data Visualization: Revisiting Javascript?
data-visualization python r
updated 14 months ago by 43k • written 11.5 years ago by 5.8k
82
votes
32
replies
7.8k
views
13 follow
Forum: Shall We Go Back To Stackexchange?
meta
updated 14 months ago by 43k • written 11.5 years ago by ★ 3.6k
6
votes
5
replies
7.8k
views
Forum: Converting GFF to GTF
GTF GFF
updated 11 months ago by 43k • written 4.2 years ago by 8.6k
5
votes
6
replies
7.7k
views
Forum: Msc Bioinformatics Project Ideas
project
updated 15 months ago by 43k • written 12.0 years ago by ▴ 50
21
votes
8
replies
7.6k
views
Forum: Is there ever a good reason to use a log transformation instead of Box-Cox?
data skew transformation power
updated 2.5 years ago by 100k • written 2.5 years ago by ★ 27k
72
votes
18
replies
7.5k
views
17 follow
Forum: What Is One Book Every Bioinformatician Should Read?
books
updated 14 months ago by 43k • written 10.9 years ago by 8.5k
65
votes
27
replies
7.5k
views
14 follow
Forum: What is the reason for most software errors in Bioinformatics according to you?
software-error blast sequencing SNP R
updated 13 months ago by 43k • written 8.1 years ago by ▴ 110
26
votes
12
replies
7.5k
views
6 follow
Forum: Mapping God Found ‘Scientifically Dishonest’ By Anonymous Peer Reviewers
ngs
updated 12 months ago by ▴ 10 • written 10.5 years ago by ▴ 50
31
votes
34
replies
7.5k
views
11 follow
Forum: pros- and cons- : programming skills vs. GUI
programming lecture
updated 13 months ago by 43k • written 8.5 years ago by ★ 4.7k
49
votes
27
replies
7.4k
views
10 follow
Forum: Possible augmented reality tools for bioinformatics and current problems
visualization 3D augmented-reality
updated 13 months ago by 43k • written 8.6 years ago by ▴ 90
49
votes
19
replies
7.3k
views
13 follow
Forum: Why Are There No Proteomics Questions In Biostar?
proteomics biostars
updated 15 months ago by 43k • written 13.5 years ago by ▴ 410
9
votes
1
reply
7.3k
views
Forum: Master in statistics or bioinformatics
masters career statistics
updated 11 months ago by 43k • written 10.0 years ago by ▴ 50
8
votes
1
reply
7.3k
views
Forum: What are chromatin states ?
genome ChIP-Seq
3 months ago by rohitsatyam102 ▴ 860
2
votes
3
replies
7.3k
views
Forum: How To Correctly Speed Up Blast Using Num_Threads
blast blast-plus
updated 14 months ago by 43k • written 10.3 years ago by ▴ 40
12
votes
7
replies
7.2k
views
Forum: Differences between SMART-seq2, SMART-seq3, and 10x
cell smartseq UMIs single 10x
updated 7 weeks ago by ★ 6.0k • written 2.7 years ago by ▴ 70
113
votes
53
replies
7.2k
views
16 follow
Forum: Middle author, but not first author projects. How would you approach this?
authorship career-development
updated 11 months ago by 43k • written 5.1 years ago by ▴ 170
22
votes
16
replies
7.2k
views
9 follow
Forum: Large Tables In Supplementary Pdf In Journal Articles
publication
updated 14 months ago by 43k • written 10.9 years ago by ▴ 520
12
votes
9
replies
7.1k
views
9 follow
Forum: Bioinformatics and wet lab science combined
career
updated 13 months ago by 43k • written 8.9 years ago by ▴ 40
6
votes
16
replies
7.1k
views
6 follow
Forum: Immune repertoire sequencing community
sequencing repseq immunology tcr ig
updated 2.2 years ago by 43k • written 9.8 years ago by 3.5k
18
votes
13
replies
7.1k
views
7 follow
Forum: Being co-first author for two nature series journals, what kinds of job can I find ?
phd
updated 18 months ago by 43k • written 7.5 years ago by ▴ 90
15
votes
6
replies
7.0k
views
Forum: What to look for in a potential postdoc?
genomics Postdoc
updated 14 months ago by 43k • written 9.9 years ago by ▴ 140
0
votes
0
replies
7.0k
views
Forum: Optimal approach for variant calling with 3 diluted germline sample in one sequencing - Capture Sequencing
germline Calling Variant Capture Sequencing
13 months ago by Alexandre Eeckhoutte • 0
40
votes
11
replies
7.0k
views
9 follow
Forum: "An Explosion Of Bioinformatics Careers" - from Science Careers
career
updated 14 months ago by 43k • written 9.9 years ago by 3.2k
14
votes
5
replies
7.0k
views
Forum: Seurat vs Monocle
R RNA-Seq next-gen single-cell
updated 11 months ago by 43k • written 4.9 years ago by 3.2k
20
votes
24
replies
7.0k
views
6 follow
Forum: Python or R
RNA-Seq alignment next-gen-sequencing
updated 11 months ago by 43k • written 6.0 years ago by ▴ 160
13
votes
1
reply
6.9k
views
Forum: The Ensembl Vep For Easy Analysis Of Your Variants
ensembl VEP variation
updated 14 months ago by 43k • written 10.4 years ago by 23k
28
votes
14
replies
6.9k
views
9 follow
Forum: How Do You Use Cloud Computing For Bioinformatics In 2013?
cloud
updated 14 months ago by 43k • written 10.9 years ago by 11k
28
votes
1
reply
6.9k
views
Forum: Bedops: The Fastest, Most Scalable, And Easily-Parallelizable Genome Analysis Toolkit!
bedops genome-analysis
updated 14 months ago by 43k • written 10.9 years ago by 35k
66
votes
21
replies
6.9k
views
18 follow
Forum: Is there anyone who was a pure biologist?
next-gen
updated 14 months ago by 43k • written 10.1 years ago by ▴ 100
29
votes
4
replies
6.9k
views
Forum: Biostar Vs. Seqanswers: What Is The Difference?
biostars
updated 15 months ago by 43k • written 11.0 years ago by ★ 1.1k
1,000 results • Page 3 of 20
Recent Votes
Answer: Calculate allelic frequency from VEP output vcf file
Answer: How can you *consistently* download BioProject IDs from NCBI's BioSample databas
Answer: Cluster sub-set analysis using Seurat
Answer: Cannot install bwa-mem2 via conda
Answer: Cluster sub-set analysis using Seurat
Answer: featureCounts output summary assigned value higher than uniquely mapped reads fr
Answer: Doubt about the process of annotation, detection, identification and classificat
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Recent Replies
Answer: Calculate allelic frequency from VEP output vcf file by ramiro.barrantes • 0
Found the answer here!! https://github.com/Illumina/strelka/blob/master/docs/userGuide/README.md#somatic Although this leads to a str…
Comment: Using VEP annotation output as the input for a second VEP annotation by Ram 43k
That's not a valid reason to delete the post. If that information was sensitive, posting it here was a mistake in the first place. I don't …
Answer: In one PCA plot, can I calculate the percentage of different factors that contri by marco.barr ▴ 110
Hi, regardless of how you generated the data whether with DESeq2 or other pipeline, your differential expression matrix can be used as inpu…
Comment: Filtering VCF files based on VAF giving incorrect results by Pierre Lindenbaum 161k
> Do you think my commands were correct? I'm not familiar enough with this bcftools syntax. but what if there is no AD, what if both…
Answer: How can you *consistently* download BioProject IDs from NCBI's BioSample databas by Ash ▴ 10
I know this was asked several years ago, but finding this post helped me solve my own problem and in the process I solved this one too. …
Answer: DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts i by swbarnes2 14k
Would Parkinson's be expected to have an effect on blood cells? Neurons, sure, but blood cells?
Comment: Doubt about the process of annotation, detection, identification and classificat by MarcosCosta • 0
Thank you very much, you've helped me a lot. Could you suggest a book or article that offers a complete explanation of these processes?
Comment: Filtering VCF files based on VAF giving incorrect results by Arton • 0
Thank you. I can't try a completely new tool but I will keep your command for future use. Do you think my commands were correct? For exampl…
Comment: Add stats to boxplot in R by Ghada • 0
Thanks. Thats work
Comment: Genotyping sites with N in reference genome by Zhenyu Zhang ★ 1.2k
Whether N -> A/T/G/C represents a variant is heavily dependent on the tool itself, and I know most tools don't. Since you don't see it in y…
Comment: calculating genomic coverage/ base overlap in R by marco.barr ▴ 110
The code seems correct at first glance... How did you generate the data in the Excel file? Have you checked the exon annotations? You could…
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU by GenoMax 142k
Did you remeber to unzip/untar the `nt` preformatted files that you downloaded?
Comment: Genotyping sites with N in reference genome by shpak.max ▴ 50
Just to clarify, I have set additional sites in the reference genome to 'N', which means (based on your statement) that these will not be g…
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU by Pierre Lindenbaum 161k
see https://www.biostars.org/p/9496674/ <!-- when i runed blastn(version 2.15) and nt database(latest version). I used aspera to download…
Answer: Genotyping sites with N in reference genome by Pierre Lindenbaum 161k
> Does UnifiedGenotyper skip/ignore sites where the reference genotype is N yes. The variant is in YOUR data, not in the reference where '…
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