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Comment: Per base sequence content failed miserably
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If this is 16S sequencing just follow the standard workflows like Qiime2.https://docs.qiime2.org/2024.2/tutorials/overview/
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Thanks changing the tmp dir fixed the out of disk space error for me as well.
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Download services for PDB are described on this page: https://www.rcsb.org/docs/programmatic-access/file-download-services
It could be as …
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Since BUSCO results are almost identical but the assembly sizes differ significantly, I suppose that the difference between the assemblies …
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nhaus
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Thank you very much for your answer, that was very helpful!
> Impossible to say. It depends on your data, particularly on how the donors …
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This could be because they are probably merging two or more datasets. If you are merging two or more datasets, it will be very important th…
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I sorted this out by first removing white space in the first line of the reference genome. Then indexed, the ran the mapper.pl script follo…
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Just to be certain, you are saying that if OP runs `samtools view` without the explicit reference it will be automatically downloaded from …
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That SQ M5 is chr1 in my copy of GRCh38 (Homo_sapiens.GRCh38_full_analysis_set_plus_decoy_hla.fa).
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Please do not add answers unless you're answering the top level question. Instead, use `Add Comment` or `Add Reply` as appropriate. I've mo…
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Does anybody has the answer to this? I also want to resquiggle my data with Remora but do not quite get which is the appropriate com…
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It's also worth mentioning `Barrnap` is in the bacterial annotation system **Prokka**.
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See also https://www.uniprot.org/help/negative_datasets
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Thank you for your response
As I can understand this tool takes sequence input files right? I have genome-wide genotypes instead of sequen…
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me
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Ok I made this into an answer. Could you please accept it as such.
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