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There are two sequences per entry, and the provided awk script has merged the two sequences into a single linear sequence. But the objectiv…
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Using @yokofakun 's [**linearize fasta code**][1]
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I used 'blastn-short', but it still resulted in a segmentation fault. I downloaded the 'nt_euk' index from NCBI ([enter link description he…
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Please check my answer. No need to send an email to EBI either.
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**Anonymous Aspera download from ENA works as expected.**
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What percentage of your library do you expect to be "long-non-coding transcripts"?
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Thank You so much....it was really helpful...i was able to remove the poly G with fastp
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Hi,
Thanks, it was helpul information. I used fastp to remove the poly g and the QC seems to be fine now.
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