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122,212 results • Page
702 of 2445
Sort: Rank
Rank
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Votes
Replies
1
vote
6
replies
6.1k
views
Compare boxplot with Wilcoxon test
boxplot
wilcoxon
ggplot
ggpubr
updated 4.7 years ago by
Alex Reynolds
36k • written 4.7 years ago by
FKM
• 0
0
votes
0
replies
1.0k
views
How to test the effect of covariate (Ex. Age) on a significant PERMANOVA test of beta diversity?
microbiome
PERMANOVA
Adonis
updated 2.7 years ago by
Ram
45k • written 4.7 years ago by
ST
• 0
0
votes
2
replies
1.1k
views
MSA score function
alignment
updated 4.7 years ago by
seidel
11k • written 4.7 years ago by
QAZ
▴ 20
2
votes
1
reply
4.1k
views
How to deal with (combine) technical replicates (FPKM vs Read count data) for RNA-Seq experiments?
RNA-Seq
replicates
read count
gene expression
updated 4.7 years ago by
Biostar
20 • written 7.7 years ago by
Ameya Kulkarni
▴ 10
0
votes
0
replies
831
views
Problem in making database for metabarcoding using OBITools
software error
4.7 years ago by
saeedmn2005
• 0
1
vote
2
replies
1.4k
views
How to save DEXSeq result to a .csv file ?
R
RNA-Seq
updated 3 months ago by
louiseadore0808
▴ 10 • written 4.7 years ago by
Aynur
▴ 60
6
votes
4
replies
1.1k
views
How to create a genome assembler?
Assembly
updated 4.7 years ago by
Mensur Dlakic
★ 30k • written 4.7 years ago by
alienzj
▴ 30
0
votes
1
reply
1.2k
views
How many HSPs does blastn returns by default?
blastn
blast
alignment
hsp
updated 4.7 years ago by
Mensur Dlakic
★ 30k • written 4.7 years ago by
langziv
▴ 70
0
votes
2
replies
1.4k
views
BBMap Clumpify: Exception in thread "Thread -#"
genome
Assembly
alignment
duplicate
updated 4.7 years ago by
Mensur Dlakic
★ 30k • written 4.7 years ago by
aman.akash2008
• 0
6
votes
5
replies
1.7k
views
paired end data with varying read length
RNA-Seq
illumina
insert size
updated 4.7 years ago by
linyao
• 0 • written 6.2 years ago by
manjumoorthy95
▴ 60
2
votes
3
replies
1.4k
views
Muscle MSA program
alignment
4.7 years ago by
QAZ
▴ 20
1
vote
0
replies
741
views
Contigs in Embls
embl
fasta
updated 4.7 years ago by
Biostar
20 • written 4.9 years ago by
estoakes91
▴ 10
0
votes
2
replies
1.8k
views
Bam splitting index .sbi ?
alignment
index
gatk
updated 4.7 years ago by
Pierre Lindenbaum
166k • written 4.7 years ago by
quentin54520
▴ 120
2
votes
1
reply
1.3k
views
I am getting error in this command while doing my analysis, please help me to understand this
rna-seq
R
updated 4.7 years ago by
rpolicastro
13k • written 4.7 years ago by
rishav513
▴ 30
1
vote
1
reply
3.5k
views
Gviz: How I can add or retrive gene symbol from TxDb.Hsapiens.UCSC.hg38.knownGene
genome
updated 4.7 years ago by
ruiyan_hou
• 0 • written 5.0 years ago by
greatgene719
▴ 10
5
votes
3
replies
2.6k
views
error in R
software-error
R
updated 3.0 years ago by
Ram
45k • written 10.4 years ago by
zizigolu
★ 4.4k
1
vote
1
reply
1.2k
views
gene expression x phenotype correlation
RNA-Seq
updated 4.7 years ago by
Gordon Smyth
★ 8.6k • written 4.8 years ago by
demoraesdiogo2017
▴ 120
3
votes
1
reply
1.7k
views
Snakemake Checkpoints Aggregate issues
snakemake
python
wildcards
4.7 years ago by
susheelbhanu
• 0
2
votes
2
replies
2.1k
views
Interpreting first round of maker
maker
busco
annotation
updated 4.7 years ago by
Dave Carlson
★ 2.2k • written 4.7 years ago by
gilsorek12
▴ 10
2
votes
4
replies
4.6k
views
how to make correlation heatmap
heatmaps
correlation
updated 4.7 years ago by
Eugene A
▴ 190 • written 4.7 years ago by
adR
▴ 130
0
votes
0
replies
1.3k
views
Job:
Senior Bioinformatician - AstraZeneca (Cambridge, UK)
cheminformatics
biopharma
genomics
updated 2.7 years ago by
Ram
45k • written 4.7 years ago by
Sergio Martínez Cuesta
▴ 230
0
votes
5
replies
2.8k
views
few genes were annotated to GO terms using online eggnog-mapper
GO
KEGG
eggnog-mapper
updated 4.7 years ago by
Biostar
20 • written 4.8 years ago by
zhangdengwei
▴ 210
1
vote
3
replies
1.1k
views
Extracellular Vesicle RNA Seq - Abundance of No Feature reads and how to investigate
RNA-Seq
4.7 years ago by
russell.stewart.j
▴ 30
0
votes
3
replies
4.1k
views
TypeError: an integer is required (got type bytes)
python
exac
gnoamd
hail
4.7 years ago by
Bala Hatun
• 0
1
vote
9
replies
2.4k
views
calculate statistically significant miRNA and mRNA target pairs using pearsons correlation
miRNA
correlation
pearson
miRNA-mRNA
updated 4.7 years ago by
i.sudbery
22k • written 4.7 years ago by
shaden
▴ 20
0
votes
0
replies
1.3k
views
Job:
Bioinformatics internships and co-ops (2021) at Stoke Therapeutics. Remote locations in the US.
internship
co-op
updated 2.7 years ago by
Ram
45k • written 4.7 years ago by
Eric Lim
★ 2.2k
5
votes
5
replies
1.6k
views
Question: how to get low quality read from sam file
RNA-Seq
alignment
updated 4.7 years ago by
ATpoint
90k • written 4.7 years ago by
minghuiguo448
• 0
2
votes
6
replies
5.6k
views
Weird Tophat2 Error Message
tophat2
updated 4.7 years ago by
Biostar
20 • written 12.3 years ago by
Nick
▴ 290
1
vote
4
replies
1.4k
views
Saving variable number of objects to .xls file
R
output data
xls
updated 4.7 years ago by
rpolicastro
13k • written 4.7 years ago by
luca
▴ 70
0
votes
4
replies
1.1k
views
What to do when having differenet versions of ensembl IDs?
R
Bioconductor
RNA-Seq
updated 4.7 years ago by
rpolicastro
13k • written 4.7 years ago by
Omar Mohamed
• 0
4
votes
6
replies
14k
views
Problem using a custom blast database in tblastx
blast
software error
updated 4.7 years ago by
nikhilshinde0909
• 0 • written 11.5 years ago by
arronar
▴ 290
0
votes
0
replies
1.0k
views
GSEAPY Enrichr Question
enrichment
4.7 years ago by
bcopeland64
• 0
0
votes
1
reply
4.8k
views
Blastx: FASTA-Reader: Ignoring invalid residues at position(s): On line 25975584
RNA-Seq
assembly
4.7 years ago by
nikhilshinde0909
• 0
0
votes
8
replies
1.2k
views
How to demultiplex SmartSeq2 run where settings were set to one index instead of two?
RNA-Seq
sequencing
4.7 years ago by
RhiRhiO
• 0
0
votes
0
replies
582
views
MetaData extraction of Sequences
genome
4.7 years ago by
inebrahim99
• 0
1
vote
4
replies
945
views
Time course data analysis
RNA-Seq
NGS
4.7 years ago by
Andrew Liu
▴ 10
9
votes
5
replies
9.0k
views
Simulating Rna-Seq Reads
rna
alignment
simulation
updated 4.7 years ago by
Biostar
20 • written 15.0 years ago by
Gww
★ 2.7k
1
vote
1
reply
1.4k
views
Visualizing FIMO Output
FIMO
RNA
Motif
Visualization
updated 4.7 years ago by
Alex Reynolds
36k • written 4.7 years ago by
gordian.knot0
• 0
11
votes
8
replies
14k
views
Question: Convert Ensembl Transcript Ids Ensmust To Gene Symbol In R
R
ENSEMBL
gene
updated 4.1 years ago by
Kevin Blighe
★ 90k • written 4.7 years ago by
sugus
▴ 150
0
votes
5
replies
1.8k
views
Convert absolute count into TPMs, merge exons or average gene length?
RNA-Seq
gene
updated 4.7 years ago by
Mensur Dlakic
★ 30k • written 4.7 years ago by
cyntsc10
• 0
0
votes
3
replies
1.5k
views
clipOverlap from BamUtil modifies CIGAR string => error in GATK
rna-seq
updated 4.7 years ago by
lechu
▴ 20 • written 4.8 years ago by
Juke34
9.3k
0
votes
0
replies
2.1k
views
"htsjdk.samtools.SAMFormatException: Error parsing text SAM file. Not enough fields" wrong message in the chromHMM run
chromHMM
SAMFormatException
4.7 years ago by
lhaiyan3
▴ 80
3
votes
2
replies
4.5k
views
BLAST Global alignment
alignment
blast+
dna
blastn
emboss
updated 4.7 years ago by
GenoMax
154k • written 4.7 years ago by
James Sacco
▴ 10
2
votes
3
replies
1.1k
views
How to remove repeats on galaxy
alignment
repeats
galaxy
updated 4.7 years ago by
Biostar
20 • written 4.9 years ago by
pt.taklifi
▴ 70
0
votes
0
replies
976
views
Job:
HIRING: Nanopore Production Specialist - UC Santa Cruz Genomics Institute
nanopore
Genomics
sequencing
updated 2.7 years ago by
Ram
45k • written 4.7 years ago by
genomicshr
▴ 30
2
votes
3
replies
1.6k
views
gencode gtf file derived geneID can't be annotated to gene symbol following Deseq2 manual
R
RNA-Seq
gene
4.7 years ago by
Kai_Qi
▴ 130
1
vote
2
replies
2.7k
views
How to split Double or Multiple Nucleotide Polymorphisms (DNP) into Single Nucleotide Polymorphisms (SNP)?
SNP
variant calling
vcf
updated 4.7 years ago by
Biostar
20 • written 8.6 years ago by
rafa.rios.50
▴ 60
1
vote
5
replies
1.6k
views
qiaseq panel fastq to vcf
ngs
qiaseq
freebayes
4.7 years ago by
tadeja.klade
▴ 10
4
votes
5
replies
3.0k
views
Whole blood single cell RNA-seq dataset?
RNA-Seq
scRNA-Seq
whole blood
single cell
2.6 years ago by
predeus
★ 2.1k
1
vote
7
replies
2.9k
views
lftp mput 530 Login incorrect
software error
4.7 years ago by
leranwangcs
▴ 150
122,212 results • Page
702 of 2445
Recent Votes
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what cause poly-G from NextSeq
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A: what cause poly-G from NextSeq
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How to trim transcripts using information from NCBI contamination screen report
How to trim transcripts using information from NCBI contamination screen report
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Recent Replies
Answer: How excactly is the Q30 is calculated?
by
GenoMax
154k
[**Phred quality scores**][1] have been in use since the 1990s and early days of human genome project. They were co-opted in the fastq form…
Comment: best practice for diploid variant calling
by
Matteo Ungaro
▴ 130
@kevin thanks I was experimenting with the following since I already pursued most of the approaches you mentioned and, in practice, they ar…
Answer: Chip-seq analysis Diffbind
by
ATpoint
90k
I think the results are expected. a) n=2 is underpowered, for any assay and in particular for ChIP-seq which has considerable noise. b) you…
Comment: low quality data or file name swapping -- cellranger arc errors when processing
by
Wu-Sheng Zhang
▴ 10
Hi @arup , after changing the file name following the read length, I can process them using cellranger arc successfully. Thank you very muc…
Comment: Visualize methylation status in Pacbio Hifi read
by
cmdcolin
★ 4.4k
(as the JBrowse dev) I'm glad to hear this. if you see any trouble with it feel free to let me know, I have tried to keep up to date with m…
Comment: low quality data or file name swapping -- cellranger arc errors when processing
by
Wu-Sheng Zhang
▴ 10
Got it! I will try to switch their name and re-run the cellranger arc. Thank you very much, @arup !
Answer: low quality data or file name swapping -- cellranger arc errors when processing
by
Arup Ghosh
3.5k
The barcode files with 24nt read length W71_LUNGrep2_S6_L001_R3_001.fastq.gz and W71_LUNGrep2_S6_L002_R3_001.fastq.gz should be the R2.
Comment: ID unifiying across different datasets
by
GenoMax
154k
Each database has their own method of annotation/validation. Depending on resources available there may be a lag between the databases in t…
Comment: low quality data or file name swapping -- cellranger arc errors when processing
by
Wu-Sheng Zhang
▴ 10
Hi @arup , thank you very much for your suggestions, and here are the results -- they seems correct. May I have your suggestions? Thank you…
Comment: Why did the QV value decrease significantly after using YAHS for HiC scaffolding
by
samuel.a.odonnell
▴ 640
I don't believe merqury ignores softmasked (lower case) nucleotides so I don't think that is the case. From what it sounds like, during th…
Comment: ID unifiying across different datasets
by
zizigolu
★ 4.4k
Thanks a lot to be helpful I’m trying to build a complete `UniProtID` to `GeneSymbol` lookup table and I’m running into a wall. I’ve alrea…
Comment: strong appearing variant not found by haplotypecaller in -ERC mode and deepvaria
by
thomas.heigl.ibk
▴ 20
thanks for the quick reply. i posted the screenshot. gatk-internally, there is still the difference using -ERC or not, priot is getting …
Comment: strong appearing variant not found by haplotypecaller in -ERC mode and deepvaria
by
thomas.heigl.ibk
▴ 20
here the igv view. showing the histogram for all samples plus the reads for one: ![enter image description here][1] and expanded view of …
Comment: low quality data or file name swapping -- cellranger arc errors when processing
by
Arup Ghosh
3.5k
Check the read lengths to make sure the files are correct.
Comment: strong appearing variant not found by haplotypecaller in -ERC mode and deepvaria
by
Pierre Lindenbaum
166k
> Any suggestions or help you could share please, show us an IGV screenshot of the position GATK realign the reads locally, discards read…
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