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Recent Replies
Answer: from CRAM to fastq
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Pierre Lindenbaum
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if the CRAM/BAM is paire-end, it **must** be sorted on query name using samtools collate
https://x.com/lh3lh3/status/1132756684789768202
…
Comment: from CRAM to fastq
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machaalani29
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one of the issues is that I am not very sure how the sequencing was done, but I think it was single end.
any suggestions hoot do that?
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Possibly. You appear to have written the data to a single file (in case it was paired-end to begin with). Check to make sure secondary alig…
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https://www.biostars.org/u/18990/ : Please do not delete threads that have received a comment/answer.
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Activities with `cucurbitacin A`: https://www.ebi.ac.uk/chembl/web_components/explore/activities/STATE_ID:RHxG8uDfrtbmiyaY55ic-w%3D%3D
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Hello,
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Thank you for your reply. I am still struggling with the first step, namely "select a compound" . can you be more specific with this step? …
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What databases are you using that are creating such large index files?
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Cannot be answered without more details. At least add accessions and commands.
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