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116,959 results • Page
1 of 2340
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What's next after GATK variant calling pipeline?
GATK
pipeline
figures
DNA-seq
just now by
mgranada3
▴ 30
0
votes
0
replies
8
views
Add line under stat test
stats
R
16 minutes ago by
Ghada
• 0
1
vote
5
replies
725
views
Converting IGV to Figure using Illustrator (or other tools)
Illustrator
IGV
updated 2 hours ago by
Craft
• 0 • written 6 months ago by
Daniel
▴ 30
0
votes
0
replies
27
views
DOCK6 installation on MacOS M1
installation
troubleshooting
DOCK6
updated 1 hour ago by
GenoMax
142k • written 2 hours ago by
Rodolfo Adrián
• 0
1
vote
3
replies
85
views
Genotyping sites with N in reference genome
GATK
UnifiedGenotyper
updated 2 hours ago by
Zhenyu Zhang
★ 1.2k • written 3 hours ago by
shpak.max
▴ 50
0
votes
1
reply
62
views
Can 5' and 3' scRNAseq be processed with the same pipeline?
singlecell
seurat
10XGenomics
scRNAseq
updated 3 hours ago by
yura.grabovska
▴ 70 • written 3 hours ago by
ev97
▴ 20
0
votes
2
replies
68
views
Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NULL > pointer.
blast
meta
how-to
ncbi
forum
2 hours ago by
hophuquy0944
• 0
797
votes
167
replies
143k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 3 months ago by
Biostar
2.8k • written 7.4 years ago by
Istvan Albert
100k
0
votes
0
replies
44
views
In one PCA plot, can I calculate the percentage of different factors that contribute to the PCA?
PCA
R
VARIANCE
3 hours ago by
diqixiaoyaoer
▴ 10
0
votes
2
replies
79
views
Sequencing Depth (Read Depth) Calculations
depth
updated 3 hours ago by
GenoMax
142k • written 4 hours ago by
jiahangkui1234
• 0
0
votes
1
reply
60
views
Wrong ZYGOSITY by ensembl vep on DeepVariant vcf file
annotation
vcf
vep
zygosity
deepvariant
3 hours ago by
asalimih
▴ 60
0
votes
0
replies
45
views
Is it okay to assemble my 12 metatranscriptome samples with Trinity separately?
trinity
metatranscriptome
4 hours ago by
jway
• 0
1
vote
2
replies
96
views
Cannot install bwa-mem2 via conda
conda
mamba
python
code
updated 4 hours ago by
Istvan Albert
100k • written 5 hours ago by
jsmith120f
• 0
2
votes
2
replies
131
views
genome finishing
finishing
genome
updated 5 hours ago by
nd48
▴ 20 • written 8 hours ago by
trezini
• 0
1
vote
5
replies
148
views
Circos Plot to Visualize Chromatin Accessibility Changes from ATAC-seq Data
circos
atacseq
updated 5 hours ago by
Pierre Lindenbaum
161k • written 5 hours ago by
biology_inform
▴ 50
1
vote
4
replies
169
views
Add stats to boxplot in R
stats
R
1 hour ago by
Ghada
• 0
0
votes
0
replies
55
views
Seurat v5 and how to correctly integrate across multiple experiments
Seurat
scRNA
R
updated 5 hours ago by
ATpoint
82k • written 6 hours ago by
yura.grabovska
▴ 70
0
votes
0
replies
46
views
Difference in number of reads when using duplex and simplex base calling with Dorado
base
calling
nanopore
simplex
dorado
duplex
6 hours ago by
Lélé
▴ 10
0
votes
3
replies
93
views
Tool:
Converting Degree Minutes (DM) to Decimal Degrees (DD) using python script
Minutes
Decimal
Degrees
python
Degree
offtopic
updated 6 hours ago by
Joe
21k • written 6 hours ago by
kuttibiotech2009
▴ 30
0
votes
3
replies
124
views
calculating genomic coverage/ base overlap in R
bioinoformatics
genomics
updated 2 hours ago by
marco.barr
▴ 110 • written 7 hours ago by
Sayantani
• 0
0
votes
2
replies
77
views
Annotating file using bcftools
annotation
plink
bcftools
updated 7 hours ago by
Pierre Lindenbaum
161k • written 8 hours ago by
kl
▴ 10
0
votes
8
replies
246
views
Duplicated reads (IDs) from nanopore sequencing
bam
nanopore
sequencing
updated 4 hours ago by
GenoMax
142k • written 13 hours ago by
njornet
▴ 20
0
votes
3
replies
101
views
Problem with Calling Variants from RNA-Seq data
vcf
gatk
benchmark
giab
rna-seq
8 hours ago by
Esraa
• 0
0
votes
1
reply
117
views
DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts in whole blood: is it normal to always get NS?
rstudio
DEG
limma
updated 7 hours ago by
ATpoint
82k • written 9 hours ago by
egascon
• 0
1
vote
1
reply
94
views
Getting differential ChIP-seq peaks between conditions after calling consensus peaks
MAnorm
ChIP-seq
consensus
peaks
updated 10 hours ago by
ATpoint
82k • written 11 hours ago by
Alexandra
• 0
1
vote
1
reply
177
views
Differential Expression with limma: Contrast and Design Matrix, combinatorial approach valid?
limma
updated 11 hours ago by
dariober
14k • written 1 day ago by
Holly
▴ 10
1
vote
2
replies
119
views
Filtering VCF files based on VAF giving incorrect results
bcftools
GATK
Bioinformatics
picard
VCF
20 minutes ago by
Arton
• 0
0
votes
0
replies
55
views
ReactomeGSA
ReactomeGSA
11 hours ago by
Shaimaa Gamal
▴ 10
0
votes
0
replies
50
views
News:
New course: Manipulation of NGS Data for Genomic and Population Genetics Analyses
KeepLearning
TScourses
11 hours ago by
Transmitting Science communication
▴ 70
0
votes
1
reply
93
views
bbmerge (bbmap) ~ error with insert size file output
bbtools
bbmerge
bbmap
updated 8 hours ago by
GenoMax
142k • written 13 hours ago by
chrisk
• 0
0
votes
1
reply
99
views
perseus software (version 1.5.5)
preseus
mass
spectrometry
data
updated 6 hours ago by
Joe
21k • written 15 hours ago by
iqra
• 0
0
votes
0
replies
67
views
Why the shape of RCS is contradictory to the p value for nonlinearity.
RCS
ggplot2
ggplot
R
updated 17 hours ago by
GenoMax
142k • written 18 hours ago by
Dude
• 0
0
votes
0
replies
64
views
NEURON, Brian2, NEST, or PyNN
NEST
NEURON
PyNN
Brian2
18 hours ago by
brunofelicianodeomena
• 0
1
vote
2
replies
156
views
Alphafold 3 pLDDT coloring scheme in chimerax?
ChimeraX
Alphaphold
Alphaphold3
updated 12 hours ago by
colindaven
6.4k • written 20 hours ago by
mbrav005
• 0
0
votes
1
reply
116
views
Polish a large genome with Pilon
Pilon
Pacbio
Polish
Illumina
15 hours ago by
sansan_96
▴ 90
1
vote
2
replies
129
views
Doubt about the process of annotation, detection, identification and classification of transposable elements
annotation
TransposableElements
32 minutes ago by
MarcosCosta
• 0
2
votes
2
replies
173
views
Genome Visualization Tools
bacterial
genome
updated 23 hours ago by
GenoMax
142k • written 23 hours ago by
dlera.lozano
▴ 10
2
votes
3
replies
182
views
RSeQC : infer_experiment.py Error: Could not retrieve index file
RSeQC
RNA-Seq
updated 22 hours ago by
Ram
43k • written 1 day ago by
Prawesh
• 0
0
votes
3
replies
232
views
Using ggplotly in R
ggplot
ggplotly
updated 4 hours ago by
jared.andrews07
★ 16k • written 1 day ago by
jen
▴ 10
0
votes
1
reply
139
views
Why does assigning genes with biomart give me different values than using a transcripts_to_genes.txt file?
biomart
RNAseq
kallisto
updated 1 day ago by
GenoMax
142k • written 1 day ago by
bioinfo
▴ 150
0
votes
3
replies
226
views
RNAseq RNA content
mRNA
Linux
rRNA
RNA-seq
RNA
updated 1 day ago by
noodle
▴ 580 • written 1 day ago by
doramora
▴ 10
0
votes
1
reply
126
views
Post-imputation QC for input into GWAS analyses
gwas
prs
updated 1 day ago by
LauferVA
4.2k • written 1 day ago by
graeme.thorn
▴ 100
0
votes
2
replies
158
views
Using Cutadapt to trim adapters from paired-end small RNA sequence data
trimming
adapters
cutadapt
sRNA-seq
smallRNA
11 hours ago by
melissa.joubert
• 0
0
votes
0
replies
98
views
Calculate allelic frequency from VEP output vcf file
VEP
variant
1 day ago by
ramiro.barrantes
• 0
4
votes
1
reply
183
views
Extracting only 4-fold degenerate sites from gene sequences/alignments?
alignments
1 day ago by
J.
▴ 40
0
votes
0
replies
96
views
Is there a real ground truth for CNV data?
CNV
1 day ago by
jennyp0706
• 0
0
votes
3
replies
194
views
Biomart issue, why so few 3'utrs?
utr
biomart
updated 1 day ago by
GenoMax
142k • written 1 day ago by
RNAseqer
▴ 270
1
vote
1
reply
130
views
sci-RNA-seq
Seurat
sci-RNA-seq
updated 1 day ago by
Ram
43k • written 1 day ago by
kilcdincer
▴ 10
4
votes
4
replies
2.9k
views
CNVkit for somatic copy number detection
cnv
cnvkit
exome
WES
updated 1 day ago by
Anitha
• 0 • written 5.1 years ago by
stephaniem
• 0
1
vote
7
replies
363
views
Overlapping Ranges within Granges object
Genomicranges
IRanges
GRanges
16 hours ago by
ntsopoul
▴ 60
116,959 results • Page
1 of 2340
Recent Votes
Answer: featureCounts output summary assigned value higher than uniquely mapped reads fr
Answer: Doubt about the process of annotation, detection, identification and classificat
Answer: Filtering VCF files based on VAF giving incorrect results
Problems with breakdancer (sv caller) output.
Problems with breakdancer (sv caller) output.
Comment: Add stats to boxplot in R
Answer: Genotyping sites with N in reference genome
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Recent Replies
Comment: Doubt about the process of annotation, detection, identification and classificat
by
MarcosCosta
• 0
Thank you very much, you've helped me a lot
Comment: Filtering VCF files based on VAF giving incorrect results
by
Arton
• 0
Thank you. I can't try a completely new tool but I will keep your command for future use. Do you think my commands were correct? For exampl…
Comment: Add stats to boxplot in R
by
Ghada
• 0
Thanks. Thats work
Comment: Genotyping sites with N in reference genome
by
Zhenyu Zhang
★ 1.2k
Whether N -> A/T/G/C represents a variant is heavily dependent on the tool itself, and I know most tools don't. Since you don't see it in y…
Comment: calculating genomic coverage/ base overlap in R
by
marco.barr
▴ 110
The code seems correct at first glance... How did you generate the data in the Excel file? Have you checked the exon annotations? You could…
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
GenoMax
142k
Did you remeber to unzip/untar the `nt` preformatted files that you downloaded?
Comment: Genotyping sites with N in reference genome
by
shpak.max
▴ 50
Just to clarify, I have set additional sites in the reference genome to 'N', which means (based on your statement) that these will not be g…
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
Pierre Lindenbaum
161k
see https://www.biostars.org/p/9496674/ <!-- when i runed blastn(version 2.15) and nt database(latest version). I used aspera to download…
Answer: Genotyping sites with N in reference genome
by
Pierre Lindenbaum
161k
> Does UnifiedGenotyper skip/ignore sites where the reference genotype is N yes. The variant is in YOUR data, not in the reference where '…
Comment: Can 5' and 3' scRNAseq be processed with the same pipeline?
by
yura.grabovska
▴ 70
Do you have samples that should in theory have a good degree over overlap in terms of cell types across the two sequencing types? I would …
Comment: Wrong ZYGOSITY by ensembl vep on DeepVariant vcf file
by
asalimih
▴ 60
I found out that vep is only checking the GT field therefore assigning HOM to the Zygosity. Therefore the problem should be from deepvarian…
Comment: Add stats to boxplot in R
by
Lélé
▴ 10
To remove "Kruskall Wallis" you can try adding the argument label="p" or label = "p.signif" which displays the p-value or *** like this: …
Answer: Sequencing Depth (Read Depth) Calculations
by
GenoMax
142k
You could also use `pandepth` ([**LINK**][1]) or `mosdepth` ([**LINK**][2]) as well. These will allow you to be flexible about windowed cov…
Comment: Sequencing Depth (Read Depth) Calculations
by
Pierre Lindenbaum
161k
it looks looks ok to me. <!-- This cmd works and gives me 40.5235, I was wondering whether this cmd is right or wrong, and does anyone hav…
Comment: Add stats to boxplot in R
by
Ghada
• 0
Great. thats work. Do you know how can I remove the kruskall-Wallis name from the plot? Also I would like to draw a line under the kruskall…
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