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121,212 results • Page
1 of 2425
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Votes
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0
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2
replies
156
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European Variation Archive submission eva-sub-cli validation failure: "Sample name concordance check"
eva-sub-cli
EuropeanVariationArchive
submission
database
updated 1 hour ago by
Timothee
• 0 • written 1 day ago by
askhat
• 0
0
votes
0
replies
36
views
Job:
Project Research Scientist-I (Bioinformatics – Next Generation Sequencing Data Analysis)
Computational
Biology
Bioinformatics
2 hours ago by
Rajunaik Vankudavath
• 0
0
votes
0
replies
41
views
News:
online course: AI for Genomics: from CNNs and LSTMs to TRANSFORMERS
Transformers
CNN
Genomics
LSTMs
AI
6 hours ago by
Physalia-courses
★ 2.6k
0
votes
1
reply
50
views
Anyone familiar with studies on computational neuroscience focused on consciousness, particularly in anatomical areas such as ARAS?
neuroscience
computational
updated 47 minutes ago by
Bastien Hervé
6.3k • written 8 hours ago by
Dun
• 0
0
votes
0
replies
43
views
Evaluating Large CRISPR/Cas9 Deletions (~300 kb) in F0 Xenopus Embryos
CRISPR
large
mosaic
Cas12a
F0
deletion
9 hours ago by
Santex
• 0
2
votes
10
replies
288
views
Trim CDS to ORF
codon
CDS
offset
transcripts
updated 41 minutes ago by
1769mkc
★ 1.3k • written 20 hours ago by
jaredbernard
▴ 30
837
votes
170
replies
177k
views
112 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 23 days ago by
Biostar
3.5k • written 8.5 years ago by
Istvan Albert
102k
0
votes
1
reply
81
views
Multi-omic trajectory on CITE-Seq data
multiomic
10x
single-cell
transcriptomics
trajectory
updated 4 hours ago by
ATpoint
88k • written 16 hours ago by
firestar
★ 1.7k
8
votes
19
replies
458
views
STAR aligner problem on local laptop
RNA-sequencing
updated 20 minutes ago by
GenoMax
151k • written 1 day ago by
Morris_Chair
▴ 370
0
votes
0
replies
89
views
Tool:
Grabstract: Turn complex scientific texts and PDFs into graphical abstracts, scientific posters, journal club slide decks, and more!
visualisation
3 hours ago by
Anand
• 0
3
votes
8
replies
1.2k
views
Mageck RRA is listing negative controls as high fold change in the gene summary
python
crispr
mageck
updated 11 hours ago by
jared.andrews07
★ 18k • written 11 months ago by
liz.b
• 0
0
votes
1
reply
91
views
RNA-seq analysis
STAR
HiSat2
featurecounts
updated 18 hours ago by
rfran010
★ 1.5k • written 19 hours ago by
SEJAL
• 0
0
votes
0
replies
59
views
CNVs annotation after CNVkit and clinical databases
Annotation
CNVs
clinical_database
19 hours ago by
AIMAR
▴ 10
1
vote
2
replies
127
views
Obtaining Q30/Q60 Values from Fastq Files
phred
quality
illumina
rna-seq
updated 21 hours ago by
GenoMax
151k • written 22 hours ago by
joe_genome
▴ 50
0
votes
1
reply
386
views
Phylogeny aware alignment input
HoSeq
MSA
updated 22 hours ago by
zjq6666666
• 0 • written 11 months ago by
Meto
• 0
0
votes
5
replies
196
views
The allele with index 2 is not defined in the REF/ALT columns" error after AF filtering with gnomAD Exome
gnomAD
bcftools
vcf
19 hours ago by
erikatatianacs
• 0
0
votes
2
replies
142
views
Drastic drop in RNA-seq read mapping rate when disabling gaps in Bowtie2
bowtie2
updated 18 hours ago by
GenoMax
151k • written 1 day ago by
triplee0305
▴ 10
2
votes
9
replies
349
views
RNASeq bulk transcriptomics analysis
RNASeq
Trimgalore
genecounts
STAR
fastqc
3 hours ago by
rajdeepboral00
▴ 60
0
votes
1
reply
110
views
SSL and Syntax Errors when using fasterq-dump (SRA Toolkit)
sratoolkit
updated 1 day ago by
GenoMax
151k • written 1 day ago by
QURROTU AINI
• 0
0
votes
2
replies
156
views
Download GenomicSEM reference file
GenomicSEM
updated 1 day ago by
1769mkc
★ 1.3k • written 1 day ago by
zhangmyharper
• 0
9
votes
10
replies
373
views
How common is it to split fastq files prior to bwa mem to increase parallelization?
mem
bwa
alignment
updated 21 hours ago by
ATpoint
88k • written 1 day ago by
curious
▴ 880
0
votes
0
replies
98
views
News:
UCSC Xena Webinar Series: Visualize and Analyze Bulk Sequencing Data on Xena
visualization
genomics
cancer
1 day ago by
Mary Goldman
• 0
0
votes
0
replies
101
views
Deseq2 DE analysis of host-pathogen samples (model separately or jointly?)
normalization
deseq2
host-pathogen
rna-seq
1 day ago by
r-ninja
▴ 20
0
votes
0
replies
99
views
Does vg stats support gaf file?
vg
1 day ago by
Wang Cong
▴ 20
1
vote
1
reply
137
views
Longest Protein per Gene from gpff file
Protein
gpff
updated 1 day ago by
lieven.sterck
15k • written 1 day ago by
reza
▴ 300
2
votes
4
replies
645
views
Modify read groups in BAM file
samtools
BAM
picard
DRAGEN
updated 1 day ago by
jkbonfield
★ 1.3k • written 6 weeks ago by
Alex
• 0
2
votes
1
reply
149
views
Integration of NanoString CosMx FOVs
Spatial
transcriptomics
updated 1 day ago by
Bastien Hervé
6.3k • written 2 days ago by
solo.albif
• 0
0
votes
1
reply
153
views
Bulk RNA-seq differential gene expression analysis on haplotype‑resolved diploid plant assembly
RNA-seq
updated 17 hours ago by
rfran010
★ 1.5k • written 1 day ago by
Zephy
• 0
0
votes
1
reply
354
views
News:
9th Berlin Summer School in NGS Data Analysis 2025 (June 30 - July 4, 2025) -- SUMMER IN BERLIN --
Workshop
RNA-Seq
transcriptomics
VariantCalling
DNA-Seq
2 days ago by
ecSeq Bioinformatics
▴ 20
0
votes
0
replies
131
views
q-value threshold for omics data when performing univariate cox analysis
MachineLearning
q-value
Cox
FDR
omics
2 days ago by
Yugan Gogul Muthukumar
▴ 10
6
votes
8
replies
443
views
Cross-species differential gene expression, finding orthologs
orthologs
xSpecies
stringtie
Differential-expression
1 day ago by
fe94
▴ 10
0
votes
3
replies
256
views
Unexpected negative correlation between gene-length and counts
rna-seq
bias
normalisation
updated 18 hours ago by
rfran010
★ 1.5k • written 2 days ago by
Ben
▴ 20
0
votes
1
reply
200
views
Perturb seq
perturbseq
updated 2 days ago by
GenoMax
151k • written 3 days ago by
shlo12
• 0
4
votes
4
replies
1.5k
views
Where can I find somatic whole-genome or exome FASTQ files (from tumor samples) with validated variants and corresponding VCFs publicly available?
fastq
NGS
pipeline
INDELs
SNVs
updated 2 days ago by
GenoMax
151k • written 6 days ago by
aaaaaaa
• 0
1
vote
1
reply
256
views
How important are allele fractions when calling variants?
GATK
bacteria
BCFTools
updated 1 day ago by
Jeremy Leipzig
23k • written 3 days ago by
andean
• 0
0
votes
0
replies
251
views
Job:
Two postdoctoral positions in generative AI and blood-based cancer detection, Aarhus University, Denmark
nucleosomes
post_doc
cancer_detection
generativeAI
bioinformatics
2 days ago by
Jakob Skou
• 0
0
votes
2
replies
1.6k
views
Analysing Problem Seen In MEGA X
Alignment
MEGAX
Muscle
updated 4 days ago by
humarafique.1234
• 0 • written 3.6 years ago by
ankurhalder116
• 0
0
votes
0
replies
244
views
discrepancies in ORA results between g:Profiler (gost) and clusterProfiler (enrichGO)
Analysis
Enrichment
written 4 days ago by
BobMarleyEnrichment
• 0
3
votes
2
replies
345
views
DESeq2 outliers-should I be concerned?
deseq2
updated 2 days ago by
ATpoint
88k • written 4 days ago by
bioinfo
▴ 150
1
vote
2
replies
394
views
Trimmomatic command
STAR
RNASeq
Trimmomatic
updated 4 days ago by
swbarnes2
14k • written 6 days ago by
rajdeepboral00
▴ 60
1
vote
1
reply
300
views
Blog:
Setaria Pan-Genome Variation: Unraveling the Story of Domestication
pan-genome
updated 4 days ago by
cmdcolin
★ 4.2k • written 6 days ago by
Novogene
▴ 510
4
votes
9
replies
1.1k
views
Facing issue with output of nextflow pipeline
output
nextflow
fastqc
issue
updated 3 days ago by
colindaven
7.4k • written 6 weeks ago by
harsh
▴ 20
6
votes
2
replies
366
views
Forum:
Pie Charts: to use or not to use
pie-chart
graphs
updated 5 days ago by
Joe
22k • written 5 days ago by
littlebioinformatician
• 0
1
vote
1
reply
260
views
Minimum Number of Cells for Downstream Analysis after subsetting a particular cell type (scRNAseq)?
cell
single
updated 5 days ago by
ATpoint
88k • written 5 days ago by
Tundup
▴ 10
0
votes
3
replies
550
views
Discrepancy Between Reported Nitrogen-Fixing Ability and Genomic Evidence in Acinetobacter guillouiae
nif
NCBI_genome_database
Acinetobacter_guillouiae
Nitrogenase
Nitrogen_fixation
updated 4 days ago by
GenoMax
151k • written 5 days ago by
Nafi
• 0
0
votes
1
reply
266
views
Target prediction
protien
prediction
Target
updated 5 days ago by
dthorbur
★ 3.0k • written 5 days ago by
Samprath
• 0
6
votes
12
replies
866
views
Heatmap of a transformed deseq object with pheatmap
deseq2
pheatmap
5 days ago by
caroline.zanchi
• 0
0
votes
0
replies
208
views
CNVkit CNVs call with threshold issues
CNVkit
threshold_log2
Somatic_variant
5 days ago by
AIMAR
▴ 10
2
votes
4
replies
435
views
PRS result interpretation
risk
score
PRS
interpretation
polygenic
1 day ago by
trkfs
▴ 10
2
votes
1
reply
298
views
More rare variants than common variants
PLINK
GWAS
updated 6 days ago by
LChart
4.9k • written 6 days ago by
RT
▴ 20
121,212 results • Page
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Recent Votes
Comment: BCL files conversion to FASTQ without SampleSheet.csv
Comment: STAR aligner problem on local laptop
Comment: STAR aligner problem on local laptop
Comment: STAR aligner problem on local laptop
Comment: STAR aligner problem on local laptop
Answer: Integration of NanoString CosMx FOVs
Answer: Integration of NanoString CosMx FOVs
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Recent Replies
Comment: STAR aligner problem on local laptop
by
GenoMax
151k
In case this suggestion does not work try the solution posted here: https://github.com/alexdobin/STAR/issues/1265#issuecomment-1595135934
Comment: STAR aligner problem on local laptop
by
GenoMax
151k
> With conda there is no problem Then why are you trying to compile locally. Are you expecting to get more (performance) out of a locally …
Comment: STAR aligner problem on local laptop
by
dsull
★ 7.6k
OK, you need to compile add `CXXFLAGS_SIMD="-march=native"` at the end of your make command.
Comment: Trim CDS to ORF
by
1769mkc
★ 1.3k
is this chatgpt solution?
Comment: Anyone familiar with studies on computational neuroscience focused on consciousn
by
Bastien Hervé
6.3k
I think your question would better fit a biology forum like [biology.stackexchange][1] [1]: https://biology.stackexchange.com/
Answer: European Variation Archive submission eva-sub-cli validation failure: "Sample na
by
Timothee
• 0
Just for the sake of anyone else having the same issue. You need to annotate each of your files in the "Files" tab with an analysis alias…
Comment: STAR aligner problem on local laptop
by
Morris_Chair
▴ 370
No alignment if I add --readFilesCommand cat in STAR. The coding source comes from GitHUB # 1. Install brew (http://brew.sh/) # …
Comment: Trim CDS to ORF
by
i.sudbery
21k
You code looks fine to me, from a logical perspective, but without examples to try it on, I can't say why its not working. I wonder if its …
Comment: building snpeff database for plant
by
analyst
▴ 60
Dear [lieven][1] i renamed the headers in my cds fasta file: now i don't get that CDS not found fatal error: > Protein check: Citrus_clem…
Comment: STAR aligner problem on local laptop
by
dsull
★ 7.6k
Hmm, one more suggestion: Add `--readFilesCommand cat` to your STAR command. Also, show us how you're compiling from source (what commands…
Comment: RNASeq bulk transcriptomics analysis
by
rajdeepboral00
▴ 60
The initial fastq after trimming had 107653608 reads
Comment: STAR aligner problem on local laptop
by
Morris_Chair
▴ 370
Thanks for advice here is the bam that I get https://we.tl/t-nTZhKXezQ1
Comment: STAR aligner problem on local laptop
by
Morris_Chair
▴ 370
With conda there is no problem but if I compile it from source it shows errors like this: g++-14: error: unrecognized command-line opt…
Comment: RNA-seq analysis
by
Gordon Smyth
★ 8.1k
OK now. Clearly the sequencing was unstranded. Just use featureCounts with default `strandSpecific=0`.
Comment: Multi-omic trajectory on CITE-Seq data
by
ATpoint
88k
The UMAP must be based on something, typically a PCA or something like this. So why not running slingshot on either that PCA-like dimred, o…
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