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291 results • Page 4 of 6
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2
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4
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1.3k
views
Forum: Transitioning from Wet Lab to Computational Biology as a PhD Student/Surgeon
RNA-Seq sequencing
updated 11 months ago by 44k • written 4.1 years ago by ▴ 10
13
votes
4
replies
1.3k
views
Forum: The shortcoming of shiny app in building webserver
R shiny webserver
updated 11 months ago by 44k • written 4.7 years ago by ▴ 120
2
votes
10
replies
1.3k
views
Forum: AddHealth Omics Data Source
AddHealthOmics
updated 12 months ago by 44k • written 5.5 years ago by • 0
13
votes
6
replies
1.3k
views
Forum: To Cloud or Not to Cloud - Privacy implications of genetic data sharing
security diagnostics DNA-Seq
updated 11 months ago by 44k • written 4.9 years ago by 11k
0
votes
3
replies
1.3k
views
Forum: Help cry of a M.Sc. student, who is seeking a path in Bioinformatics
PhD
updated 12 months ago by 44k • written 5.5 years ago by ▴ 50
19
votes
7
replies
1.3k
views
9 follow
Forum: DNA in pixels
genome alignment sequencing
updated 11 months ago by 44k • written 3.5 years ago by ▴ 30
3
votes
2
replies
1.2k
views
Forum: Preferred cloud environment for genomics.
next-gen-sequencing RNA-Seq alignment
updated 11 months ago by 44k • written 3.8 years ago by ▴ 10
2
votes
6
replies
1.2k
views
Forum: Would love to hear from you re: which bionformatics resources could use an improvement in their interface.
seq database gene prot
updated 11 months ago by 44k • written 4.1 years ago by • 0
2
votes
9
replies
1.2k
views
Forum: open source projects looking for contributors
open-source
updated 8 months ago by ▴ 70 • written 8 months ago by ▴ 20
2
votes
7
replies
1.2k
views
Forum: Future prospects: NGS vs Gene editing (using CRISPR CAS9, AAVs and similar methods)
NGS CRISPR-Cas9
updated 11 months ago by 44k • written 4.6 years ago by ▴ 170
4
votes
4
replies
1.2k
views
Forum: What skills are required for an informatics scientist?
informatics scientist
updated 11 months ago by 44k • written 5.0 years ago by ★ 9.4k
19
votes
5
replies
1.2k
views
6 follow
Forum: What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2023?
genomics snps survey papers
5 months ago by Raony Guimarães ★ 1.4k
5
votes
4
replies
1.2k
views
Forum: Consider CodeOcean or other solutions for runnable Biostars answers?
CodeOcean how-to meta
updated 11 months ago by 44k • written 4.0 years ago by 22k
0
votes
2
replies
1.2k
views
Forum: Welcome to join our Wechat group for Bioinformatics!
single-cell ChIP-Seq genome algorithm RNA-Seq
updated 11 months ago by 44k • written 3.7 years ago by ▴ 160
33
votes
12
replies
1.2k
views
7 follow
Forum: Nextflow and self-made pipelines opinion
nextflow pipeline data-analysis
12 weeks ago by karlaarz ▴ 110
0
votes
0
replies
1.2k
views
Forum: Interest in joining a bioinformatics project
project
updated 12 months ago by 44k • written 5.3 years ago by ▴ 10
16
votes
10
replies
1.2k
views
6 follow
Forum: F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 7 weeks ago by 5.3k • written 8 weeks ago by ★ 2.4k
4
votes
0
replies
1.2k
views
Forum: Plotting Ensembl gene name changes between versions
R ensembl
updated 11 months ago by 44k • written 4.8 years ago by ▴ 600
1
vote
3
replies
1.2k
views
Forum: R-programming: individual lessons or Institutes where to learn and practice in Germany
R biologist
updated 12 months ago by 44k • written 5.5 years ago by ▴ 10
1
vote
2
replies
1.2k
views
Forum: Metagenomics Tutoring Opportunity
metagenomics microbial tutoring
updated 12 months ago by 44k • written 5.4 years ago by ▴ 30
2
votes
2
replies
1.2k
views
Forum: Freshman puzzled by tons of statistic methods
ML biostatistics statistics
updated 12 months ago by 44k • written 5.6 years ago by ▴ 30
0
votes
0
replies
1.1k
views
Forum: ssDNA Aptamer structure prediction
aptamer selex
updated 11 months ago by 44k • written 4.0 years ago by • 0
0
votes
2
replies
1.1k
views
Forum: Will de novo protein structure prediction research become irrelevant?
protein-structure-prediction
updated 12 months ago by 44k • written 5.4 years ago by ▴ 110
4
votes
5
replies
1.1k
views
Forum: A silly question about journal clubs
journal-club challenge
updated 11 months ago by 44k • written 4.2 years ago by ▴ 280
7
votes
4
replies
1.1k
views
Forum: [Meta] Use of AI
AI Artificial-Intelligence meta ChatGPT
updated 11 months ago by 44k • written 11 months ago by 4.2k
7
votes
9
replies
1.1k
views
Forum: DEG analysis when not replicates are available
NOISeq RNA-Seq edgeR
updated 11 months ago by 44k • written 4.1 years ago by ★ 5.1k
3
votes
2
replies
1.1k
views
Forum: Which is the best tutorial site to learn practical approaches on NGS?
next-gen-sequencing R
updated 12 months ago by 44k • written 5.3 years ago by ▴ 20
5
votes
6
replies
1.1k
views
Forum: COVID19 testing - can we/should we use NGS?
NGS covid19 diagnostics
updated 11 months ago by 44k • written 4.2 years ago by ★ 1.4k
3
votes
3
replies
1.1k
views
Forum: Large Multi-omics Project -- Dev Approach
snp RNA-Seq sequencing
updated 11 months ago by 44k • written 4.8 years ago by ▴ 10
6
votes
5
replies
1.1k
views
Forum: A free temporary HPC service for bioinformatic analysis
HPC
updated 11 months ago by 44k • written 4.2 years ago by ▴ 340
0
votes
5
replies
1.1k
views
Forum: Advice for a start in Bioinformatics
R next-gen Python
updated 11 months ago by 44k • written 4.0 years ago by • 0
4
votes
4
replies
1.1k
views
Forum: Kubernetes in Production?
ci-cd webservice kubernetes docker
updated 11 months ago by 44k • written 4.4 years ago by • 0
17
votes
11
replies
1.1k
views
Forum: How to overcome the imposter syndrome in bioinformatics?
advice
4 months ago by sil_bioinfo ▴ 40
2
votes
3
replies
1.1k
views
Forum: Feedback on Read Alignment Programs
alignment
updated 12 months ago by 44k • written 5.3 years ago by • 0
2
votes
0
replies
1.1k
views
Forum: How to Fairly Represent and Interpret Direct-To-Consumer Ancestry Results?
Ancestry chromosome-painting ADMIXTURE
updated 11 months ago by 44k • written 4.3 years ago by 8.2k
3
votes
0
replies
1.1k
views
Forum: Good books on application of Machine Learning in Bioinformatics
book machine-learning
updated 11 months ago by 44k • written 4.8 years ago by ▴ 180
1
vote
1
reply
1.1k
views
Forum: Companies and salary for a PhD in Drug Design in Asia
salary drug-design
updated 11 months ago by 44k • written 5.0 years ago by • 0
3
votes
5
replies
1.1k
views
Forum: Current Trends in Bioinformatics
structural-bioinformatics
updated 5 months ago by 44k • written 6 months ago by • 0
6
votes
5
replies
1.1k
views
Forum: Russian universities for the study of biomedicine
russian-university
updated 11 months ago by 44k • written 3.9 years ago by ▴ 30
3
votes
7
replies
1.0k
views
Forum: Construct a small computing server for GWAS Analysis
assembly
updated 11 months ago by 44k • written 5.0 years ago by ▴ 60
0
votes
2
replies
1.0k
views
Forum: Suffix Arrays: looking for an instructive algorithm of SA construction for teaching
suffix-arrays linear-time teaching algorithm
updated 11 months ago by 44k • written 4.1 years ago by 54k
2
votes
6
replies
1.0k
views
Forum: Error in posting to Biostars?
meta
updated 11 months ago by 44k • written 4.4 years ago by ▴ 60
0
votes
4
replies
1.0k
views
Forum: Genes inhibiting each other's expression
Gene-expression
updated 11 months ago by 44k • written 3.7 years ago by ▴ 60
3
votes
4
replies
1.0k
views
Forum: Survey - Tell me about your Bioinformatics struggles
multiomics RNA-Seq dna-seq computational-biology
updated 12 months ago by 44k • written 5.5 years ago by • 0
0
votes
3
replies
1.0k
views
Forum: costs for library preps
NGS
updated 12 months ago by 44k • written 5.4 years ago by ★ 2.6k
1
vote
2
replies
1.0k
views
Forum: Homologous sequences problematic in RNA-seq
Homology genome RNA-Seq
updated 11 months ago by 44k • written 5.2 years ago by ▴ 10
4
votes
6
replies
1.0k
views
6 follow
Forum: Bioinformatics: what do you think of it in the next 20 years?
future
updated 5 months ago by 44k • written 5 months ago by • 0
3
votes
1
reply
1000
views
Forum: Recommend papers: automated vs manual downstream analysis
downstream-analysis reproducibility
updated 11 months ago by 44k • written 5.0 years ago by ▴ 110
0
votes
2
replies
987
views
Forum: RNA seq data
RNA-Seq
updated 12 months ago by 44k • written 5.4 years ago by • 0
2
votes
5
replies
984
views
6 follow
Forum: Biostar as a next generation version
meta mobile-app
updated 7 months ago by 44k • written 8 months ago by • 0
291 results • Page 4 of 6
Recent Votes
Cellranger-multi : Demultiplexing and Analyzing 5’ Immune Profiling Libraries Pooled with Hashtags
A: Venter Genome Vcf
Answer: seqtk subseq in.fastq list.txt > out.fastq not extracting full sequence from
Answer: PCA plot no distinct cluster. Only p < 0.05 indicates significant DEGs, while ad
Random Access remote BAM files
Answer: PCA plot no distinct cluster. Only p < 0.05 indicates significant DEGs, while ad
C: Converting narrowPeak to bed
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Comment: Construction of circos plot from WGS data by Anitha ▴ 10
Thank you for the information. But, I am still struggling like which data to use and how to process the input data to represent to build th…
Comment: Tools for chromosomal aneuploidy detection by cmdcolin ★ 3.8k
is there any reason why you do not like tools based on read depths?
Comment: CreateSeuratObject taking very long by eae6d2e7 • 0
Thank you for your reply! I followed your advice to convert to sparse matrix first but I got this error Error in Matrix::sparseMatrix(raw_…
Answer: Venter Genome Vcf by ericrkofman ▴ 20
I can't find an hg38 version, and the old VCF seems to be of a different header format so I am having difficulty converting it to hg38 from…
Comment: What is a good way to do gene differentials in single cell data where one group by ATpoint 82k
Preferred if you have true biological replicates. Can still be combined with my subsetting strategy, like use 100x different cells for the …
Answer: ComplexHeatmap - How to change fontsize of rowAnnotation by ATpoint 82k
```r set.seed(1) m = matrix(rnorm(100), nrow = 10) rownames(m) = 1:10 # normal ha = rowAnnotation(foo = anno_mark(at=1:nrow(m), labels=row…
Comment: ComplexHeatmap - How to change fontsize of rowAnnotation by Ram 44k
Please do not add answers unless you're actually answering the question. I've moved your post to a comment.
Comment: ComplexHeatmap - How to change fontsize of rowAnnotation by hannes.bongartz • 0
I still cannot find a way. It seems to be an easy to solve issue and I was trying any possible combination with the aforementioned gp=gpar(…
Comment: What is a good way to do gene differentials in single cell data where one group by fracarb8 ★ 1.6k
I would probably use a pseudobulk approach.
Comment: Error when looping over multiple columns in a data frame in R by Jeremy ▴ 910
`cutpoint_results[[col_name]]` adds each new cut point to the list entitled "cutpoint_results", while keeping the original column names fr…
Answer: PCA plot no distinct cluster. Only p < 0.05 indicates significant DEGs, while ad by swbarnes2 14k
Never use uncorrected p-values with RNASeq. Your PCA is suggesting that your sample groups are not very different, and the DEG tests con…
Comment: What is a good way to do gene differentials in single cell data where one group by ATpoint 82k
I would keep it transparent. Do DE by subsetting large to small group. Do that randomly many times, then either average stats or use some s…
Answer: Trying to understand warning from MACS2 about too few paired peaks and differing by jared.andrews07 ★ 17k
Without looking at the actual sequence for each genome, my guess is that the Echinobase reference just matches much more closely to the org…
Comment: What do the transcript variant # mean in RefSeq? by Ram 44k
> It does not seem to be the longest transcript. Is it the canonical transcript? Longest == canonical. If you're looking for the transcrip…
Comment: Mouse or Rat Gene Expression Data Similar to GTEx by Shicheng Guo ★ 9.4k
Hi ATpoint, Thank you for your responses. My apologies if my inquiries have seemed one-sided. I greatly value the collaborative spirit of t…
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