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1,000 results • Page
6 of 20
Sort: replies
Rank
Views
Votes
Replies
27
votes
17
replies
22k
views
8 follow
BioPython: convert fasta to fastq without quality score input file
parsing
phred
fasta
BioPython
fastq
updated 11 months ago by
capemaster
• 0 • written 10.0 years ago by
Josh Herr
5.8k
13
votes
17
replies
11k
views
8 follow
cutadapt for trimming reads to specific length
cutadapt
trim-galore
updated 12 weeks ago by
xiaoleiusc
▴ 140 • written 7.6 years ago by
Varun Gupta
★ 1.3k
6
votes
17
replies
1.7k
views
MacOS Quicklook plugin for gtf and gff3 files?
gtf
gff3
MacOS
updated 11 months ago by
Ram
43k • written 11 months ago by
kalavattam
▴ 190
7
votes
17
replies
5.1k
views
Tool:
SPLiT-Seq Demultiplexing a bash tool for extraction of barcoded single cells
RNA-Seq
sequencing
updated 10 months ago by
Ram
43k • written 5.7 years ago by
paulranum11
▴ 80
20
votes
17
replies
3.3k
views
How to add "transcript" feature to a gtf file?
gtf
updated 10 months ago by
1769mkc
★ 1.2k • written 3.3 years ago by
pomodoro_sinensis
▴ 110
36
votes
17
replies
3.7k
views
9 follow
Which Software Development Technique Is Used In Your Lab?
subjective
updated 6 months ago by
Ram
43k • written 14.2 years ago by
Giovanni M Dall'Olio
28k
6
votes
17
replies
1.7k
views
Filtering rows based on a list if all the entries of the list correspond to a specific row value
row-matching
sorting
filtering
updated 11 months ago by
Ram
43k • written 12 months ago by
bionix
▴ 10
17
votes
17
replies
21k
views
13 follow
How To Efficiently Sort A Fastq File By Entry Id?
fastq
illumina
sort
next-gen-sequencing
alignment
updated 4 months ago by
Ram
43k • written 12.5 years ago by
User 9996
▴ 840
17
votes
17
replies
2.0k
views
6 follow
Forum:
Journal for beginners
journal
updated 11 months ago by
Ram
43k • written 6.2 years ago by
heref
▴ 20
6
votes
17
replies
5.6k
views
8 follow
Eigen genes using WGCNA
wgcna
eigengenes
updated 11 months ago by
chaco001
▴ 40 • written 5.7 years ago by
shivangi.agarwal800
▴ 120
6
votes
17
replies
6.7k
views
Can you add a label key to heatscatter plot in R?
heatscatter
visualization
R
updated 29 days ago by
Ram
43k • written 9.6 years ago by
james.lloyd
▴ 100
36
votes
16
replies
14k
views
8 follow
How to identify 16s sequences from binning data(contigs)?
genome
Assembly
sequence
blast
updated 10 months ago by
Kermit
▴ 90 • written 8.6 years ago by
liuyifan2014
▴ 110
6
votes
16
replies
2.9k
views
Tool:
MicroScope: ChIP-seq and RNA-seq software analysis suite for gene expression heatmaps
RNA-Seq
ChIP-Seq
updated 10 months ago by
Ram
43k • written 7.6 years ago by
Bohdan Khomtchouk
▴ 350
20
votes
16
replies
20k
views
6 follow
Conversion of Gene Name to Ensembl ID
gene
RNA-Seq
R
genome
updated 6 months ago by
Nat.Nataren
▴ 10 • written 4.1 years ago by
bazok
▴ 20
34
votes
16
replies
21k
views
9 follow
Filtration Of Reads With Length Lower Than 30 From Bam
read
length
bam
updated 27 days ago by
Hagen
▴ 10 • written 10.3 years ago by
filipzembol
▴ 180
7
votes
16
replies
929
views
How many reads for WGS Sequencing?
WGS
Bacterial-Genomics
17 days ago by
Ruqaiya
• 0
16
votes
16
replies
7.7k
views
6 follow
Tool:
Oncofuse: Prediction Of Driver Gene Fusions From Ngs Data
annotation
fusion
rna-seq
ngs
updated 10 months ago by
Ram
43k • written 10.7 years ago by
mikhail.shugay
3.5k
12
votes
16
replies
11k
views
8 follow
fpkm to tpm conversion
R
next-gen
updated 3 months ago by
DareDevil
★ 4.3k • written 6.7 years ago by
sayamsmruti
▴ 20
39
votes
16
replies
37k
views
16 follow
Tutorial:
installing samtools with conda - overcoming two common errors
samtools
conda
updated 4 months ago by
GenoMax
142k • written 3.8 years ago by
lavinia.gordon
▴ 160
30
votes
16
replies
2.3k
views
9 follow
Forum:
Which programming langauge shall I start as a beginner in Bioinformatics
programming-language
single-cell
updated 11 months ago by
Ram
43k • written 4.1 years ago by
nkg.bb
• 0
9
votes
16
replies
2.3k
views
Can't liftover vcf file from hg19 to hg38
bcftools
updated 10 months ago by
Ram
43k • written 15 months ago by
Victor
▴ 10
46
votes
16
replies
5.9k
views
12 follow
News:
Google announces DeepVariant
genome
deep-learning
google
updated 12 months ago by
Ram
43k • written 6.4 years ago by
Hussain Ather
▴ 990
74
votes
16
replies
4.8k
views
10 follow
Tools & Techniques Bioinformatician Have To Master?
subjective
updated 10 months ago by
Ram
43k • written 13.2 years ago by
Thaman
★ 3.3k
3
votes
16
replies
12k
views
combining z-scores into a single z-score value
excel
R
statistics
updated 11 months ago by
Ram
43k • written 5.2 years ago by
Star
▴ 60
4
votes
16
replies
1.9k
views
How to subset large BAM files specifically/ extract specific subsets?
bamtools
RNAseq
samtools
BAM
updated 11 weeks ago by
Ram
43k • written 7 months ago by
ella
• 0
16
votes
16
replies
2.3k
views
Species identifcation using 16s rRNA
16srRNA
fasta
updated 7 months ago by
Ram
43k • written 3.2 years ago by
A_heath
▴ 160
0
votes
16
replies
3.9k
views
Gff to genbank - feature is missing
DNA
augustus
gff3
genbank
updated 11 months ago by
Ram
43k • written 5.9 years ago by
rororo
▴ 10
31
votes
16
replies
2.2k
views
8 follow
Forum:
What is in your opinion the current state-of-the-art sequencing platform for genomic assembly (Nov 2019)
next-gen-sequencing
assembly
genome
updated 11 months ago by
Ram
43k • written 4.5 years ago by
Antonio R. Franco
★ 5.1k
15
votes
16
replies
4.8k
views
Gene Expression Experiment Using Ngs Data
next-gen-sequencing
gene-expression
updated 6 months ago by
Ram
43k • written 14.1 years ago by
Eric Normandeau
11k
15
votes
16
replies
1.8k
views
ncbi error report log for validate fastq issue
sra-tools
updated 7 months ago by
GenoMax
142k • written 7 months ago by
1769mkc
★ 1.2k
34
votes
16
replies
20k
views
14 follow
Tutorial:
TCGA UUIDS to TCGA barcode (SampleID) in R
next-gen
GDC
R
TCGA
updated 8 weeks ago by
aUser
▴ 30 • written 7.6 years ago by
martinguerrerog89
▴ 310
2
votes
16
replies
6.3k
views
Converting Microarray SNP data into VCF format
Affymetrix
SNP
VCF
RNA-Seq
Microarray
updated 12 months ago by
Xu
• 0 • written 5.4 years ago by
Susmita Mandal
▴ 110
3
votes
16
replies
1.8k
views
Simulate reads for positions in a vcf file
variant-calling
simulation
bam
vcf
updated 7 months ago by
Brian Bushnell
20k • written 10 months ago by
berndmann
▴ 10
11
votes
16
replies
8.0k
views
6 follow
Frequency of a unique sequence in a fasta file
sequencing
FASTA
updated 9 months ago by
Shashi
• 0 • written 9.2 years ago by
Tanvir Ahamed
▴ 350
16
votes
16
replies
5.7k
views
how to run a tool (package) installed into anaconda environment ..using mac terminal
genome
updated 11 months ago by
Ram
43k • written 4.0 years ago by
Pegasus
▴ 100
8
votes
16
replies
2.9k
views
Tool:
CuteVcf : A Qt application to view VCF file
htslib
gui
vcf
tabix
Qt
updated 10 months ago by
Ram
43k • written 7.4 years ago by
sacha
★ 2.4k
28
votes
16
replies
31k
views
11 follow
How Do I Convert From Bed Format To Gff Format?
gff
galaxy
bed
updated 7 months ago by
Ram
43k • written 14.6 years ago by
Istvan Albert
100k
3
votes
16
replies
1.5k
views
How to bring all reads to the same length? Any tool?
Read-length
Bed
updated 11 months ago by
Ram
43k • written 4.3 years ago by
Ankit
▴ 500
8
votes
16
replies
5.3k
views
9 follow
visualize CNV data based on WGS
CNV
updated 8 weeks ago by
clealk
▴ 50 • written 6.3 years ago by
Bogdan
★ 1.4k
10
votes
16
replies
17k
views
7 follow
How Can I Count Snps In My Final Vcf Files
SNP
updated 9 days ago by
Pierre Lindenbaum
161k • written 5.8 years ago by
mostafarafiepour
▴ 180
42
votes
16
replies
34k
views
14 follow
Generating consensus sequence from bam file
genome
samtools
bwa
fasta
updated 7 months ago by
jkbonfield
★ 1.2k • written 5.2 years ago by
chparada
▴ 70
10
votes
16
replies
1.6k
views
Filter gene with low count in RNA-seq using a function from edgeR
edgeR
RNA-seq
updated 9 months ago by
petebio
▴ 100 • written 9 months ago by
Chris
▴ 280
3
votes
16
replies
2.7k
views
design matrix for dge
limma
differential-gene-expression
r
updated 5 weeks ago by
Ram
43k • written 8.0 years ago by
1234anjalianjali1234
▴ 50
6
votes
16
replies
2.1k
views
help in converting a table
RNA-seq
updated 10 months ago by
Ram
43k • written 6.1 years ago by
Chris
▴ 30
0
votes
16
replies
1.1k
views
What is the sequence origin in human plasma?
human
sequence
cfDNA
4 weeks ago by
biwdpang
• 0
5
votes
16
replies
2.0k
views
MEME calling motifs from eCLIP-seq file generate no information
software-error
RNA-Seq
next-gen
ChIP-Seq
updated 11 months ago by
Ram
43k • written 4.1 years ago by
Kai_Qi
▴ 130
17
votes
16
replies
14k
views
10 follow
How to extract the longest isoform from multi fasta file
perl
extract
fasta
python
isoform
updated 6 weeks ago by
Madeleine
• 0 • written 9.8 years ago by
jon.brate
▴ 310
18
votes
16
replies
1.9k
views
Forum:
Why are people not working on gene prioritization and candidate gene selection for new experiment design?
gene-prioritization
updated 11 months ago by
Ram
43k • written 5.6 years ago by
prasadhendre
▴ 20
4
votes
16
replies
1.0k
views
hisat2 location does not exist
hisat2
updated 11 weeks ago by
Ram
43k • written 12 weeks ago by
Eric
• 0
50
votes
16
replies
14k
views
12 follow
Tool:
Omictools: A didactic directory for omic data analysis (NGS, microarray, PCR, MS, NMR)
pcr
software
ngs
updated 11 months ago by
Ram
43k • written 10.7 years ago by
arnaud.desfeux
▴ 450
1,000 results • Page
6 of 20
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Scale total height phylogenetic tree
Scale total height phylogenetic tree
Comment: finding evidence(s) of a peptide translated from an "Upstream Open Reading Frame
Answer: Would you bother re-mapping RNA-seq data from an old GRCh38 build to a newer ver
Answer: finding evidence(s) of a peptide translated from an "Upstream Open Reading Frame
MAFtools variant classes mapping
Answer: Would you bother re-mapping RNA-seq data from an old GRCh38 build to a newer ver
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Comment: In one PCA plot, can I calculate the percentage of different factors that contri
by
marco.barr
▴ 120
ok, then you should add the donor information to the PCA analysis. First create a separate dataframe for the donors, then add with `factor`…
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b.contreras.moreira
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If you are willing to do some coding I think <https://github.com/smarco/WFA2-lib> is among the fastest global aligners, the repo has C++ ex…
Comment: finding evidence(s) of a peptide translated from an "Upstream Open Reading Frame
by
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142k
There is an API that you could try: https://www.ebi.ac.uk/pride/markdownpage/prideutilities
Comment: finding evidence(s) of a peptide translated from an "Upstream Open Reading Frame
by
Pierre Lindenbaum
161k
thanks. looks interesting but it's broken. the Firefox console shows a 404 for https://www.ebi.ac.uk/pride/ws/archive/v2/peptidesummary?ke…
Answer: finding evidence(s) of a peptide translated from an "Upstream Open Reading Frame
by
GenoMax
142k
Perhaps: https://www.ebi.ac.uk/pride/peptidome/ This site is very slow. Almost to the point of being not usable. My search with your pepti…
Comment: Can I perform a correlation test with 3 biological replicates per condition?
by
ATpoint
82k
You're welcome. Yes, unfortunately you need quite a sample size for WGCNA simply because correlations with few samples are vastly underpowe…
Comment: Can I perform a correlation test with 3 biological replicates per condition?
by
manuelmourato25
• 0
Ah, I see. So seen that what I want is the per-gene correlation, the minimum of 20/25 for the pearson correlation still applies. Thank you …
Answer: Can I perform a correlation test with 3 biological replicates per condition?
by
ATpoint
82k
I think you're misunderstanding some concepts here. WGCNA is a framework that defines modules based on how and which genes are correlated …
Comment: Would you bother re-mapping RNA-seq data from an old GRCh38 build to a newer ver
by
GenoMax
142k
> most up-to-date build Major build of the genome remains GRCh38. What you are looking at is the latest patch release for that build. Only…
Comment: Can I perform a correlation test with 3 biological replicates per condition?
by
manuelmourato25
• 0
Thank you @atpoint . My apologies, maybe I should rephrase the question. I read the WGCNA docs already, and for the Pearson correlation tes…
Comment: How to extract cells of different species after mapping with combined genome?
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GenoMax
142k
Can you confirm that this answer is specific for `singleron` technology and associated bioinformatics software.
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GenoMax
142k
What OS are you using? Are you able to run `blastn --help` and does it produce the in-line help? > I used many query file.fasta And tho…
Comment: Can I perform a correlation test with 3 biological replicates per condition?
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ATpoint
82k
> However, even though 3 biological replicates per condition seems to be the norm in these experiments, many papers discuss that we need at…
Comment: DEG analysis with limma and contrast matrix using multiple Parkinson's cohorts i
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> I have curiosity, why do you make a desing with interceptor and without contrast matrix? Because for a 2-group comparison you do not nee…
Comment: Would you bother re-mapping RNA-seq data from an old GRCh38 build to a newer ver
by
ATpoint
82k
If you have a one-click workflow ready and the necessary computation power to casually run it, then go for it. If it is any greater effort …
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