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970 results • Page
7 of 20
Sort: replies
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Views
Votes
Replies
1
vote
4
replies
1.3k
views
Comparing RNA-Seq data to online databases
RNA-Seq
batch-effect
updated 19 days ago by
Ram
43k • written 4.7 years ago by
Victoria
▴ 10
3
votes
4
replies
403
views
How to download large-scale cDNA sequences from NCBI effectively ?
NCBI.
cDNA
updated 23 days ago by
Pierre Lindenbaum
161k • written 23 days ago by
Sony
▴ 10
1
vote
4
replies
396
views
Limma Analysis Agilent Microarray Data (GPL1708)
Microarray
Limma
Agilent
7 days ago by
hagl
▴ 10
1
vote
4
replies
389
views
Homer giving troubles
homer
HOMER
28 days ago by
naveedhasan2000
• 0
0
votes
4
replies
403
views
Ensembl stable ID cross-references
database
parsing
cross-reference
ensembl
updated 28 days ago by
Ben_Ensembl
★ 2.4k • written 4 weeks ago by
J
• 0
5
votes
4
replies
689
views
DEG analysis of RNA-seq data across multiple tissues and two conditions
RNA-seq
EdgeR
DEGs
DESeq2
17 days ago by
BioinfGuru
★ 1.7k
2
votes
4
replies
445
views
chromHMM annotations
chromHMM
28 days ago by
nswati106
• 0
2
votes
4
replies
2.6k
views
When to use .vcf or .gvcf files from GATK HaplotypeCaller?
indel
gatk
calling
snp
variant
updated 7 days ago by
Medhat
9.7k • written 24 months ago by
Vitor1
▴ 120
1
vote
4
replies
1.4k
views
Removing/Accounting for batch effects in unbalanced group RNAseq data
rna-seq
batch-effect
deseq
updated 18 days ago by
Ram
43k • written 2.3 years ago by
claysandel
• 0
0
votes
4
replies
263
views
Genbank File Format
gbkformat
6 days ago by
alenew.am
• 0
5
votes
4
replies
2.2k
views
How do we process Kallisto output (continuous real values) for DESEQ2 analysis (which strictly require discrete count values)?
read-counts
DESEQ2
differential-gene-expression
kallisto
updated 27 days ago by
Ram
43k • written 2.8 years ago by
mrj
▴ 170
4
votes
4
replies
392
views
Bowtie2 mapping is slow
metagenomics
bowtie2
mapping
read
updated 24 days ago by
colindaven
6.4k • written 4 weeks ago by
Md Moinuddin
• 0
2
votes
4
replies
2.3k
views
Negative values after batch correction using removeBatchEffect from Limma
batch
limma
removeBatchEffect
rna-seq
batch-effect
updated 19 days ago by
Ram
43k • written 21 months ago by
ev97
▴ 20
0
votes
4
replies
380
views
How to use pre-alignments by bowtie2
ATAC-seq
Bowtie2
26 days ago by
Aki
▴ 20
1
vote
4
replies
1.1k
views
Filtering qscore on dorado
dorado
filtering
QC
nanopore
Guppy
4 days ago by
eebloom
▴ 80
6
votes
4
replies
2.2k
views
Is there a way to get RNAFold output for multifasta in tabular format?
RNAFold
updated 19 days ago by
jakobjung
• 0 • written 4.6 years ago by
adhirajnath14
▴ 40
1
vote
4
replies
1.7k
views
Batch correction and DE analysis of miRNA Seq data
miRNAseq
Differential-Expression
batch-effect
updated 18 days ago by
Ram
43k • written 5.1 years ago by
georgians
▴ 50
4
votes
4
replies
445
views
How do I install plink2 on a mac?
plink2
plink
updated 21 days ago by
zx8754
11k • written 24 days ago by
emilija.romic
▴ 10
1
vote
3
replies
265
views
TaxID mapping file
annotation
blobtools
RNAseq
decontamination
transcriptomes
1 day ago by
Lada
▴ 30
3
votes
3
replies
324
views
How do I find up-to-date gene symbols for outdated names
names
symbols
gene
29 days ago by
DSmith
• 0
0
votes
3
replies
283
views
rRNA depletion of RIP-seq samples
rRNA
RIP-seq
updated 26 days ago by
noodle
▴ 580 • written 27 days ago by
CrisRisu
• 0
2
votes
3
replies
979
views
Method to detect genome doubling
CNV
genome doubling
updated 14 days ago by
LauferVA
4.2k • written 4.5 years ago by
CY
▴ 750
7
votes
3
replies
2.9k
views
Tutorial:
Prediction of Ribosomal RNA Genes Using RNAmmer Software
Annotation
rRNA
RNAmmer
Genome
updated 17 days ago by
colindaven
6.4k • written 16 months ago by
Dr.Animo
▴ 130
3
votes
3
replies
230
views
Genbank vs SRA
ncbi
genbank
genomics
sra
updated 14 days ago by
Mensur Dlakic
★ 27k • written 14 days ago by
c_u
▴ 520
1
vote
3
replies
869
views
add INFO tag to vcf with bcftools
bcftools
vcf
updated 20 days ago by
Ram
43k • written 2.0 years ago by
from the mountains
▴ 230
3
votes
3
replies
739
views
KissDE and batch effect
kissDE
kissplice
14 days ago by
david.b.rombaut
▴ 10
0
votes
3
replies
296
views
Difference between samtools "-f 9" and "-f 11"
samtools
sam
bam
updated 12 days ago by
Istvan Albert
100k • written 13 days ago by
Dylan
• 0
0
votes
3
replies
303
views
How do I handle it if there are multiple ENSEMBL IDs in one gene symbol in the scRNA sequencing data?
Seurat
scRNA-seq
count-matrix
28 days ago by
Jeyong
• 0
1
vote
3
replies
3.3k
views
How to calculate Differential Gene expression between samples with replicates
Differential-Gene-Expression
RNA-Seq
updated 27 days ago by
Ram
43k • written 7.9 years ago by
doncheng1
• 0
2
votes
3
replies
1.5k
views
RNASeq - How to compare the expression of a single gene across batches
RNA-Seq
edgeR
batch-effect
updated 18 days ago by
Ram
43k • written 3.6 years ago by
oakhamwolf
▴ 20
1
vote
3
replies
415
views
multiple sequence alignment
BLAST
multiple-sequence-alignment
clustal-omega
MSA
updated 28 days ago by
Ram
43k • written 4 weeks ago by
catherinemaria2728
• 0
2
votes
3
replies
940
views
Possible approach to select normal tissue samples for cancer RNA-Seq data without reference data for downstream analyses
R
RNA-Seq
DE
batch-effect
GTEx
updated 21 days ago by
Ram
43k • written 4.1 years ago by
svlachavas
▴ 790
7
votes
3
replies
1.2k
views
biomaRt::getSequence - Invalid type argument
biomaRt
R
updated 19 days ago by
Ram
43k • written 3.9 years ago by
mbk0asis
▴ 690
9
votes
3
replies
7.0k
views
EdgeR-GLMs correcting for batch effects
RNA-Seq
edgeR
Batch-Effect
updated 18 days ago by
Ram
43k • written 9.9 years ago by
Matina
▴ 250
0
votes
3
replies
413
views
why renaming Idents in Seurat object doesn't work?
Seurat
RenameIdents
R
6 days ago by
Assa Yeroslaviz
★ 1.8k
1
vote
3
replies
2.5k
views
Problem to remove subset of patients with plink
plink
PED
updated 13 days ago by
chrchang523
10k • written 6.2 years ago by
Ginevra
▴ 10
3
votes
3
replies
560
views
Korean human genome reference file
GWAS
reference
imputation
17 days ago by
SeoGyun
• 0
0
votes
3
replies
3.2k
views
Batch correction (mass spectrometry data)
Combat
R
SVA
Biostatistics
batch-effect
updated 18 days ago by
Ram
43k • written 7.5 years ago by
mkhadka10
• 0
5
votes
3
replies
734
views
Illumina reads preprocessing best practice for snp calling applications
Illumina
snp
updated 17 days ago by
Enrique
• 0 • written 9 months ago by
Denis
▴ 310
1
vote
3
replies
308
views
DESeq2 processing problems
deseq2
R
updated 5 days ago by
ATpoint
82k • written 5 days ago by
jagdish7921
• 0
0
votes
3
replies
1.1k
views
Discrepancy between E() Values in Blastp Search - HELP
BLAST
E-value
Blastp
Protein
updated 19 days ago by
Ram
43k • written 5.3 years ago by
minasmayth
• 0
1
vote
3
replies
278
views
ERROR When Using Ensembl Plants Biomart Wget API
biomart
plant
ensembl
14 hours ago by
Dora
▴ 10
0
votes
3
replies
537
views
python file for coding potential calculator
cpc2.py
updated 13 days ago by
atharvakarkare14
▴ 30 • written 18 days ago by
Ashok
• 0
1
vote
3
replies
234
views
DiffBind: no peaks in DBA
MACS2
DiffBind
ChIP-seq
updated 3 days ago by
jared.andrews07
★ 16k • written 4 days ago by
yvonneh
▴ 10
1
vote
3
replies
399
views
Help understanding how KEGG Ortholog `K00004 ` has 3 ECs associated with it (EC:1.1.1.4, 1.1.1.-, 1.1.1.303)?
ontology
metagenomics
database
enzymes
genomics
updated 7 days ago by
Mensur Dlakic
★ 27k • written 9 days ago by
O.rka
▴ 710
1
vote
3
replies
253
views
Bedtools merge minimum overlap?
bedtools
genomics
updated 3 days ago by
Alex Reynolds
35k • written 3 days ago by
SJP
• 0
3
votes
3
replies
643
views
Why are there several gene expression files (for example, miRNA) in TCGA for one case?
Differential-gene-expression
TCGA
miRNA
updated 27 days ago by
Ram
43k • written 5 months ago by
mohammadhassanj
▴ 260
1
vote
3
replies
308
views
How to assign cell types after integration in scRNA
scRNA-seq
updated 8 days ago by
ATpoint
82k • written 8 days ago by
Francesco
▴ 10
0
votes
3
replies
418
views
How to identify gaps in a genome?
awk
seqkit
assembly
fasta
genome
updated 13 days ago by
colindaven
6.4k • written 21 days ago by
rj.rezwan
• 0
2
votes
3
replies
359
views
clustalo - align milions of short seq, memory issue
memory
clustalo
updated 14 days ago by
Mensur Dlakic
★ 27k • written 14 days ago by
emmanouil.a
▴ 120
970 results • Page
7 of 20
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Answer: Gene ontology and homologs
Comment: anRichment is missing
Comment: Gene Specific coverage from WGS data
Comment: Gene Specific coverage from WGS data
Comment: High Malat-1 expression in single cell data
A: Meme-Tools: Bit score
Meme-Tools: Bit score
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Recent Replies
Comment: Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA
by
sovrappensiero
▴ 90
Ah, yes this makes sense! I knew this was an issue with, say, using kraken2 on shotgun metagenomic reads, but I don't have a lot of experi…
Comment: How to access TCGA samples that were treated with a specific drug?
by
Qroid
▴ 40
Thank you. Maybe it's best to confirm with the authors about how they're getting responder/non-responder labels. I'll do that now.
Comment: Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA
by
Chris Dean
▴ 390
If you sequenced a short hypervariable region, most of your sequence reads will not be able to be assigned to the species level. One possi…
Comment: anRichment is missing
by
GenoMax
142k
Consider emailing Dr. Horvath about this. Until enough people make him aware of this issue WGCNA "problem" is unlikely to get fixed.
Comment: anRichment is missing
by
Tatyana
• 0
Unfortunately, there are only tutorials and codes for tutorials for WGCNA, not installation files for any their packages... :(
Comment: anRichment is missing
by
GenoMax
142k
See this link for the WGCNA documentation and code from Peter Langfelder : https://bioinformatics.stackexchange.com/questions/21885/where-t…
Comment: anRichment is missing
by
Tatyana
• 0
The problem is back, Horwath's lab web is down again. Is it possible to get anRichment istallation file somewhere? (The greater problem is …
Comment: 16S rRNA microbiome differential abundance analysis with DESeq2 finds same micro
by
andres.firrincieli
3.6k
1. Maribaculaceae is a family not a genus 2. Who said that ASV with the same taxonomic classification must have the same behaviour? 3. With…
Comment: Question regarding WGCNA
by
andres.firrincieli
3.6k
the within-group variability is so small that these look like technical replicates rather than independent biological replicates
Answer: Gene ontology and homologs
by
geneontologyhelp
▴ 400
No. Homology is defined as shared common ancestry. Two genes that take part in the same biological process may not have the same functio…
Answer: error in R code
by
Matthias Zepper
4.6k
I quickly googled [the source code of that function][1], and the error is triggered by this condition: if(length(wn) < 1 || !is.whol…
Comment: Clarification regarding SAM flags "mate reverse strand" (flag 16/0x10) and "read
by
kalavattam
▴ 190
Thanks for the explanation. I noticed and corrected a typo in which "*read* reverse strand" initially read as "*mate* reverse strand" in t…
Comment: Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA
by
sovrappensiero
▴ 90
It's a commercial product, a *Staphylococcus hominis* genome. This species is not found in the DADA2 results, so I guess DADA2 is not ident…
Comment: Question regarding WGCNA
by
Ram
43k
Plots are incomplete without legends. Please share the complete plot with mappings for shape and color.
Comment: Quetion regarding WGCNA
by
deepak
• 0
Thank you for your reply andres.firrincieli. I have attached the PCA plot of the expression data used in the WGCNA. ![enter imag…
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