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1,000 results • Page
2 of 20
Sort: replies
Rank
Views
Votes
Replies
2
votes
12
replies
739
views
Nanopore data filtering using fastp
fastp
nanopore
28 days ago by
emilydolivo97
▴ 10
6
votes
12
replies
2.0k
views
Help with error in cellranger
Cellranger
updated 11 months ago by
GenoMax
141k • written 11 months ago by
Chris
▴ 260
0
votes
12
replies
3.3k
views
comparative analysis of two strains of bacteria's genomes
genome
gene
updated 11 months ago by
Ram
43k • written 6.5 years ago by
fonteneaudam
▴ 20
4
votes
12
replies
7.8k
views
6 follow
BWA error: no ID within the read group line
bwa
readgroups
RG
parallel
updated 11 weeks ago by
mgdrnl
▴ 10 • written 3.4 years ago by
whb
▴ 60
3
votes
12
replies
3.0k
views
What is NCBI Gene ID, where to find it and how to convert to entrez ID?
RNA-Seq
updated 10 months ago by
Pegasus
▴ 100 • written 4.6 years ago by
mnazir
▴ 10
0
votes
12
replies
628
views
BWA MEM different synthax on linux (bash) and macOS (zsh)
MEM
BWA
7 weeks ago by
gernophil
▴ 80
3
votes
12
replies
941
views
why 99% reads failed to align in mir-seq analysis?
Fastqc
bowtie1
miRNA-seq
alignment
differential-expression-analysis
3 months ago by
mohammadhassanj
▴ 260
0
votes
11
replies
2.2k
views
i want to convert .ht2 files into FASTA file
GATK
HISAT2
FASTA
updated 10 months ago by
Ram
43k • written 4.9 years ago by
saumya.sharma1509
• 0
2
votes
11
replies
5.2k
views
How long it takes to run repeat masker on a full genome
Assembly
updated 3 months ago by
e.r.zakiev
▴ 200 • written 7.8 years ago by
CAnna
▴ 20
4
votes
11
replies
1.6k
views
Check Strandedness
Check_Strandedness
how_are_we_stranded_here
leafcutter
bash
updated 6 months ago by
Brian Bushnell
20k • written 8 months ago by
Y
• 0
0
votes
11
replies
1.6k
views
count file having zero
RNA-Seq
next-gen
alignment
updated 10 months ago by
Ram
43k • written 5.2 years ago by
BioBaby
▴ 20
0
votes
11
replies
1.1k
views
Normalization for microarrays >1000+ samples?
microarray
normalization
oligo
7 months ago by
evmae
• 0
0
votes
11
replies
467
views
How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq libraries?
STARSolo
scRNA-seq
STAR
snRNA-seq
MGI
13 hours ago by
atowns21
• 0
5
votes
11
replies
997
views
Make heatmap for RNA-seq with non replicate
RNA-seq
updated 8 months ago by
Ram
43k • written 8 months ago by
Chris
▴ 260
0
votes
11
replies
776
views
Filtering VCF based on codon-transcript
vcf
bcftools
updated 5 weeks ago by
barslmn
★ 2.1k • written 7 weeks ago by
avelarbio46
▴ 30
2
votes
11
replies
771
views
Reference Guided genome assembly
tabix
updated 8 weeks ago by
Michael
54k • written 9 weeks ago by
sainavyav22
• 0
4
votes
11
replies
632
views
Remove X in row name with only number
GSVA
updated 11 weeks ago by
Ram
43k • written 11 weeks ago by
Chris
▴ 260
5
votes
11
replies
1.3k
views
Interpret IGV output inversion
variant-calling
IGV
alignment
updated 8 months ago by
cmdcolin
★ 3.8k • written 8 months ago by
pablo
▴ 300
4
votes
11
replies
1.8k
views
PCA WITH PLINK
PLINK
updated 8 months ago by
Kevin Blighe
87k • written 8 months ago by
Michelle Guerra
• 0
2
votes
11
replies
1.0k
views
Data preparation for a ML model
machine-learning
RNA-Seq
R
python
biomarkers
4 months ago by
sil_bioinfo
▴ 40
1
vote
11
replies
1.2k
views
Quantification after transcriptome assembly with Trinity
De-novo-transcriptome-assembly
Trinity
RNA-seq-analysis
updated 7 months ago by
GenoMax
141k • written 8 months ago by
langziv
▴ 50
1
vote
10
replies
1.1k
views
Do monoclonal antibodies have a blocking effect?
block
antibody
offtopic
updated 8 months ago by
Jeremy
▴ 890 • written 8 months ago by
jinyu
▴ 10
5
votes
10
replies
848
views
Error when viewing a bam file
samtools
updated 10 months ago by
Ram
43k • written 12 months ago by
Chris
▴ 260
0
votes
10
replies
959
views
ONT Raw Read Sequencing Pipeline
remora
dorado
ONT
Basecall
3 months ago by
turcoa1
• 0
1
vote
10
replies
1.7k
views
Deepvariant variant calling by singularity
deepvariant
singularity
conda
docker
updated 7 months ago by
jeffhsu3
• 0 • written 9 months ago by
fra.r.silvestro
▴ 10
1
vote
10
replies
1.2k
views
Coverage histogram query
Alignment
8 months ago by
prasundutta87
▴ 660
1
vote
10
replies
3.1k
views
How to specify/calculate subject coverage of the alignment (alignment of query[protein sequence] and subject[Nucleotide sequence]) in tBLASTn?
alignment
BLAST
tBLASTn
updated 11 months ago by
Ram
43k • written 5.1 years ago by
Kumar
▴ 120
2
votes
10
replies
3.2k
views
6 follow
Split plink files by a number of SNPs
GWAS
SNP
plink
updated 7 months ago by
Raygozak
★ 1.4k • written 5.8 years ago by
kakukeshi
▴ 80
2
votes
10
replies
3.4k
views
RepeatMasker: createLib() Error
RepeatMasker
updated 9 days ago by
Ram
43k • written 7.4 years ago by
linda
• 0
8
votes
10
replies
2.1k
views
PyWGCNA
WGCNA
10 months ago by
yoshifumimiya
▴ 40
8
votes
10
replies
698
views
bash script
whole-genome-sequencing
updated 10 days ago by
Ram
43k • written 18 days ago by
bestone
▴ 30
0
votes
10
replies
623
views
Nextflow ERROR : Timeout waiting for connection from pool
nextflow
25 days ago by
dzisis1986
▴ 70
6
votes
10
replies
1.7k
views
Integration of Microarray datasets with different platforms and biological groups
limma
microarray
DEA
batch-effect
updated 8 days ago by
Ram
43k • written 4.7 years ago by
asalimih
▴ 60
1
vote
10
replies
977
views
file conversion from gtf to gff3 for evidence modeler
gff3
stringtie
gtf
6 months ago by
rj.rezwan
• 0
2
votes
10
replies
823
views
Only one read per run - Trying to use CellRangerv7
Fastq
CellRanger
updated 28 days ago by
Ram
43k • written 9 weeks ago by
Sky
▴ 10
0
votes
10
replies
863
views
Problem running scPred
scPred
scPredict
updated 6 weeks ago by
Ram
43k • written 3 months ago by
Nitin
• 0
14
votes
10
replies
910
views
How to remove center population from seurat cluster
single-cell
4 months ago by
synat.keam
▴ 100
5
votes
10
replies
1.3k
views
the distribution of log2Fc and t value are not similar for bulk RNA-seq
decoupleR
RNA-seq
8 months ago by
alwayshope
▴ 40
3
votes
10
replies
1.2k
views
STAR index not working
STAR
RNA-Seq
7 months ago by
camillab.
▴ 160
2
votes
9
replies
820
views
TCGA2STAT Error: Firehose connection
FIREHOSE
TCGA
updated 2 days ago by
LauferVA
4.2k • written 6 months ago by
Gnana
• 0
0
votes
9
replies
1.4k
views
Samtools sam to bam error
samtools
updated 5 months ago by
Andrea
• 0 • written 2.0 years ago by
kb_93
▴ 10
3
votes
9
replies
671
views
STARsolo seg fault without readMapNumber flag
STAR
RNA-seq
STARsolo
Velocyto
updated 6 months ago by
Ram
43k • written 6 months ago by
rbronste
▴ 420
6
votes
9
replies
2.9k
views
How to analyze the scRNA seq Fastq files from NCBI
SRAtool
Cellranger
NCBI
updated 10 months ago by
Ram
43k • written 2.3 years ago by
aimanbarki
▴ 20
0
votes
9
replies
5.4k
views
Calculating FPKM and TPM by hand from htseq-count output?
RNA-Seq
rna-seq
htseq
FPKM
TPM
updated 8 months ago by
ATpoint
82k • written 5.0 years ago by
Joel Wallenius
▴ 210
1
vote
9
replies
1.2k
views
Trying to edit VCF file
VCF
R
bcftools
snpsaurus
updated 10 months ago by
Ram
43k • written 10 months ago by
peavy
• 0
3
votes
9
replies
787
views
Understanding this analysis pipeline
reads
updated 10 months ago by
Ram
43k • written 10 months ago by
artemchuki
• 0
0
votes
9
replies
480
views
Low number of both surviving reads after trimming
trimmomatic
trimming
28 days ago by
Jay
• 0
6
votes
9
replies
2.0k
views
Error when runing Bowtie2: (ERR): bowtie2-align exited with value 1
BAM
Bowtie
updated 11 months ago by
Ram
43k • written 15 months ago by
luzglongoria
▴ 50
4
votes
9
replies
1.0k
views
bcl2fastq troubleshooting all reads dumped to "Undetermined"
demultiplex
illumina
sequencing
updated 4 months ago by
GenoMax
141k • written 4 months ago by
MaxF
▴ 120
7
votes
9
replies
1.1k
views
Calculation of TMB on gene level
genomics
updated 7 months ago by
svp
▴ 680 • written 7 months ago by
smrutimayipanda
▴ 20
1,000 results • Page
2 of 20
Recent Votes
Webinar “Learning Single-cell Atlases” with Dr. Fabian Theis, author of Scanpy on May 8
biomaRt external_gene_name same entry multiple times when mapping to homolog mouse gene
Converting between Ensembl Versions
Comment: what is the purpose of indexing the reference genome (Kallisto)
Answer: what is the purpose of indexing the reference genome (Kallisto)
Answer: How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
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Recent Replies
Comment: genome assembly records not present in assembly_summary.txt
by
GenoMax
141k
Can you clarify what `metainfo` you are referring to? The accession that you refer to does exist in [**GenBank assembly_summary file**][1…
Comment: what is the purpose of indexing the reference genome (Kallisto)
by
i.sudbery
19k
Specifically: If we want to look up all the places where a 31mer is in a genome without and index, we have to compare that 31 to the first…
Answer: How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
by
benformatics
3.9k
This is way easier to do solely in R now 10 years later. library(GenomicFeatures) library(rtracklayer) ## import GTF…
Answer: what is the purpose of indexing the reference genome (Kallisto)
by
dsull
★ 5.8k
I'll see if I can explain things in super simple terms (albeit with some abuse of the actual technical details) to provide a conceptual und…
Answer: How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
by
Jalil Sharif
▴ 80
The updated code in R using rtracklayer for reading a gtf file. https://github.com/jalilsharif/gtf_utr_fix_r/blob/main/gencode_utr_fix_d…
Comment: Rare Disease Variant Pathway Analysis
by
The_PyPanda
▴ 10
Thank you LauferVA, Your advice has been useful and I now a lot to think about and read. Also, I just wanted to clarify I do not ha…
Comment: CDS vs cDNA vs transcript for mapping RNA-Seq reads
by
Antonio R. Franco
★ 5.1k
You cannot do it with Kallisto either. In fact, the indexation will fail in attempting to index a genome file
Comment: PCA plot
by
ATpoint
82k
What is your question? Add details and clarity. Ask a precise question and please avoid opening more questions on the same underlying issue…
Comment: genome assembly records not present in assembly_summary.txt
by
Joe
21k
If you have the accession numbers can you not use something like `eutils`?
Comment: Create a new bed file with all pairwise combinations between two other bed files
by
Pierre Lindenbaum
161k
> Next, I tried using bedtools window but in your original post you said > tried finding the intersection of these bed files using bedto…
Comment: How to trim transcripts using information from NCBI contamination screen report
by
Lada
▴ 30
Thank you very much! I tried it out! This is a handy tool useful for many different applications, and in regards to my problem - although …
Comment: Create a new bed file with all pairwise combinations between two other bed files
by
J
• 0
Thank you for replying Pierre. I first added a third column to the SNP bed file, so that I would get a range of 1 for each SNP awk 'B…
Comment: Create a new bed file with all pairwise combinations between two other bed files
by
Pierre Lindenbaum
161k
> using bcftools Show us what you tried
Answer: gvcf joint calling
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> Hi, guys, there is a question about the genomic gVCF file. I wonder > that since gVCF contains the non-var block records, why after merge…
Comment: Missing protein (VEGF-A) in String db
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Thank you for replying here; this helped me figure out why MAPK10 was missing from my results. It was also marked as a pseudogene in the En…
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