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Recent Replies
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Thanks! I think you might be misinterpreting your coverage output. I believe that just indicates the chromosome and how many reads map to i…
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There are two sequences per entry, and the provided awk script has merged the two sequences into a single linear sequence. But the objectiv…
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Using @yokofakun 's [**linearize fasta code**][1]
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I used 'blastn-short', but it still resulted in a segmentation fault. I downloaded the 'nt_euk' index from [NCBI][1].
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Please check my answer. No need to send an email to EBI either.
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**Anonymous Aspera download from ENA works as expected.**
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Kudos for writing such a clear and well formatted question. I will give my perspective but don't claim to be an authority. Full disclosure …
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Please email EBI and dont post duplicates. Especially not as an answer.
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What percentage of your library do you expect to be "long-non-coding transcripts"?
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