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117,466 results • Page
2 of 2350
Sort: Rank
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Votes
Replies
0
votes
2
replies
321
views
Enrichment Analysis of Pathways
Enrichment-Analysis
cytoscape
updated 2 days ago by
Scooter
▴ 280 • written 5 days ago by
Akash D
▴ 60
2
votes
5
replies
451
views
Salmon ~ Effective Length
Salmon
updated 2 days ago by
Rob
6.7k • written 18 days ago by
chrisk
• 0
2
votes
5
replies
1.1k
views
MAKER2 no FASTAs generated
MAKER
FASTA
fasta_merge
genome annotation
updated 2 days ago by
Kinoppy
• 0 • written 4.1 years ago by
jamie.pike
▴ 80
0
votes
1
reply
158
views
vg giraffe, accept admixed single and paired reads as input
vg
updated 2 days ago by
Jordan M Eizenga
▴ 480 • written 2 days ago by
Thomas
• 0
0
votes
0
replies
123
views
vg search for all specific kmer/motif (e.g., RE site) occurrences in pangenome graph
vg
2 days ago by
Thomas
• 0
2
votes
2
replies
255
views
Phasing human samples with Shapeit4
Phasing
Shapeit
Shapeit4
2 days ago by
Private
▴ 10
0
votes
3
replies
207
views
In silico PCR - multiple Primers and multiple sequences at the same time
help
pcr
pcrinsilico
updated 2 days ago by
GenoMax
143k • written 2 days ago by
sulichkarolina
• 0
1
vote
1
reply
151
views
scaling for kallisto estimate counts
Differential-gene-expression
RNAlysis
Kallisto
2 days ago by
Meghan.T
• 0
2
votes
3
replies
424
views
Submitting to GEO: 10x Fixed RNA profiling (Single Cell Gene Expression Flex)
rna-seq
updated 3 days ago by
matthewsmith
▴ 10 • written 10 days ago by
psm
▴ 130
0
votes
0
replies
158
views
Tutorial:
DiffBind run parallel
CHIP-seq
ATAC-seq
DiffBind
updated 2 days ago by
ATpoint
83k • written 3 days ago by
Juan
• 0
2
votes
1
reply
162
views
Reformatting Fastq to Fasta for Metagenomic Analysis
metagenomics
fasta
fastq
updated 3 days ago by
Ram
44k • written 3 days ago by
echolley
• 0
1
vote
1
reply
171
views
MGI DNBelab C Series High-throughput Single-cell RNA Library Preparation Set V3.0, Seurat
rna-seq
seurat
single-cell
mgi
updated 3 days ago by
bk11
★ 2.7k • written 3 days ago by
yau
• 0
0
votes
7
replies
360
views
Looking for clarification of .vcf output from variant calling
variant
calling
vcf
freebayes
variants
updated 1 day ago by
Jeremy Leipzig
22k • written 3 days ago by
Jess
▴ 20
0
votes
0
replies
117
views
KEGG pathways analysis (ko and map IDs)
ko
kegg
pathway
map
updated 3 days ago by
GenoMax
143k • written 3 days ago by
manekineko
▴ 10
0
votes
3
replies
237
views
circlize R package to plot ecDNA
ecDNA
plot
circlize
updated 1 day ago by
cmdcolin
★ 3.8k • written 3 days ago by
a.loka92
• 0
0
votes
1
reply
154
views
Determining biological relevance of a list of motifs?
motifs
HOMER
updated 3 days ago by
ATpoint
83k • written 3 days ago by
Ronin
▴ 10
0
votes
4
replies
439
views
Is using the assembled transcript in one de novo assembler as reference/trusted contigs for the second assembler logical?
Trinity
RNA-seq
rnaSPAdes
updated 3 days ago by
GenoMax
143k • written 3 days ago by
RCMC
• 0
6
votes
6
replies
339
views
Best way to create a custom reference for CellRanger when using a transgenic mouse strain expressing a human protein.
cellranger
updated 3 days ago by
ATpoint
83k • written 3 days ago by
Alx
• 0
0
votes
0
replies
111
views
RMSD vs SDM (structural distance)
RMSD
3 days ago by
Coralie
• 0
1
vote
2
replies
197
views
cluster annotation in single-cell (-nuclei) RNA-Seq or multiome experiments
celldex
annotation
singler
scRNAseq
clustering
2 days ago by
gogeni5529
▴ 50
0
votes
2
replies
517
views
Speed of Cell-Cell interaction analysis on scRNA-seq data
Cellchat
scRNA-seq
Seurat
updated 3 days ago by
gglim
▴ 160 • written 12 months ago by
miyeok
• 0
0
votes
0
replies
110
views
Overrepresentation analysis for fractions
overrepresentation
3 days ago by
malonzm1
▴ 20
1
vote
1
reply
1.5k
views
Uniprot SPARQL: InterPro domain positions
sparql
updated 3 days ago by
me
▴ 760 • written 6.1 years ago by
fabian.grammes
▴ 10
1
vote
12
replies
814
views
6 follow
blast Segmentation fault
blastn
fasta
local-blast
updated 5 hours ago by
Tina
• 0 • written 3 months ago by
giulia.cosenza
▴ 10
0
votes
2
replies
184
views
Ubuntu 24.04 for Oxford Nanopore Mk1B
Ubuntu
installation
Software
2 days ago by
Calvin-Calvin
• 0
0
votes
5
replies
222
views
sam2tsv add in my path
sam2tsv
updated 2 days ago by
Pierre Lindenbaum
162k • written 3 days ago by
harry
▴ 30
3
votes
3
replies
220
views
FastQC report
quality
fastqc
filtration
updated 2 days ago by
a.alnawfal.1992
▴ 290 • written 3 days ago by
analyst
▴ 50
0
votes
6
replies
287
views
Stuck on how to run STARsolo on paired cDNA RNA-Seq FASTQ files
STARsolo
FASTQ
1 day ago by
Nicholas
• 0
0
votes
0
replies
89
views
PCa Biplot For RNA-Seq Study Samples With 1k Genome Reference Panel
ethnicity
snp
hapmap
3 days ago by
ssankar3
• 0
1
vote
1
reply
145
views
ROH and Manhattan plot
ROH
Manhattan
plink
updated 3 days ago by
bk11
★ 2.7k • written 3 days ago by
Emy
▴ 50
0
votes
7
replies
8.2k
views
7 follow
Using bcftools to change ploidy level in vcf files
snp
bcftools
updated 3 days ago by
michael
▴ 10 • written 7.1 years ago by
flolil27
• 0
0
votes
0
replies
97
views
What is the reason for the requirement of at least 2 experiments for LFQ in MaxQuant?
MaxQuant
Spectrometry
Mass
Proteomics
LFQ
3 days ago by
Riq
▴ 50
2
votes
1
reply
148
views
Regarding WGCNA
WGCNA
updated 3 days ago by
Ram
44k • written 3 days ago by
deepak
• 0
0
votes
6
replies
329
views
Why am I getting such a big difference in alignment rates in kallisto?
kallisto
ensembl
3 days ago by
bioinfo
▴ 150
0
votes
1
reply
163
views
Reading files from different types as H5AD
python
scanpy
anndata
single-cell
updated 4 days ago by
bk11
★ 2.7k • written 4 days ago by
JACKY
▴ 140
1
vote
5
replies
2.0k
views
Error in converting bed file to interval using Picard
WES
exome
BedToIntervalList
picard
agilent
updated 3 days ago by
Ram
44k • written 4.6 years ago by
Assa Yeroslaviz
★ 1.9k
0
votes
0
replies
192
views
maftools oncoplot top bar barplots - any way to customize this plot further with color aesthetics or cutoff lines?
maf
maftools
oncoplot
exome
updated 4 days ago by
Ram
44k • written 4 days ago by
acererak
▴ 10
0
votes
4
replies
234
views
Help with CNA analysis using control-FREEC
CNA
control-FREEC
4 days ago by
sainavyav22
• 0
1
vote
0
replies
191
views
Job:
Seeking remote intership/job
remote
europe
updated 4 days ago by
Ram
44k • written 4 days ago by
giulia.cosenza
▴ 10
1
vote
2
replies
198
views
Access to Giraffe sequence data
nature
giraffe
data-access
updated 4 days ago by
Ram
44k • written 4 days ago by
Hansen_869
▴ 80
0
votes
1
reply
149
views
Best kit for PacBio HiFi Sequencing preperation
PacBio-sequencing
PacBio-HiFI
HMW-gDNA
updated 4 days ago by
Ram
44k • written 4 days ago by
Human
• 0
1
vote
1
reply
163
views
Diffbind fold values and parameter Fold in DBA.report function
DESEQ2
ATAC-seq
DBA.report
Diffbind
4 days ago by
Manko47
▴ 10
0
votes
1
reply
185
views
Conservation scores at each location of few motifs in MSA
conservation-scores
FASTA
MSA
updated 4 days ago by
zx8754
11k • written 4 days ago by
swathi.graduate1998
• 0
0
votes
1
reply
147
views
How lambda parameter works in BLAST?
lambda
BLAST
updated 3 days ago by
biofalconch
★ 1.1k • written 4 days ago by
jqg44605
• 0
6
votes
3
replies
299
views
GSEA with fgsea package and using stat from DESeq2 output - what to do with NA padj values?
RNA-seq
DESeq2
GSEA
4 days ago by
Fossil
▴ 20
0
votes
1
reply
237
views
Editing seurat object metatdata
Seurat
metadata
scRNA-seq
updated 4 days ago by
Nat.Nataren
▴ 40 • written 6 days ago by
Happiness
• 0
3
votes
1
reply
229
views
Tutorial:
How to organize a computational biology project
file-organization
updated 4 days ago by
Mensur Dlakic
★ 27k • written 4 days ago by
Ming Tommy Tang
★ 4.0k
1
vote
1
reply
200
views
Why the UK biobank switch off the data download facilities?
UKB
updated 3 days ago by
Sina
• 0 • written 4 days ago by
baimudan
▴ 10
0
votes
2
replies
286
views
How to download BLCA.focal_score_by_genes.txt from GDC
GDC
updated 4 days ago by
Zhenyu Zhang
★ 1.2k • written 10 days ago by
GAOY6429
• 0
6
votes
3
replies
289
views
Maximum bits in ggseqlogo is less then 2?
ggseqlogo
updated 5 days ago by
bk11
★ 2.7k • written 5 days ago by
Mattan
▴ 10
117,466 results • Page
2 of 2350
Recent Votes
Comment: bash for merging bam files by samtools
Alignment tool for very short reads (25bp)
Answer: edgeR batch effect model matrix sanity check
Answer: edgeR batch effect model matrix sanity check
Answer: Failed to download data from EBI with ascp
Alignment tool for very short reads (25bp)
Answer: Overrepresented sequence in reverse read
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Answer: how linearize nucleotide lines in axt file
by
GenoMax
143k
Using @yokofakun 's [**linearize fasta code**][1] awk '/^Lm/ {printf("%s%s\t",(N>0?"\n\n":""),$0);N++;next;} {printf("%s",$0);} END …
Comment: Help aligning Nanopore Long Read RNA seqs
by
WouterDeCoster
47k
That is very few reads. Are you sure your run was successful?
Comment: Alignment tool for very short reads (25bp)
by
ATpoint
83k
bowtie aka bowtie1 was developed when these very short reads were state of the art. Id give that a try and see what comes out. Multithreade…
Answer: Alignment tool for very short reads (25bp)
by
Jeremy Leipzig
22k
> What are other reliable tools for the alignment of DNA reads with very short lengths (25-50bp)? [vmatch][1] [1]: http://www.vma…
Comment: edgeR batch effect model matrix sanity check
by
kmyers2
▴ 80
Thank you! Appreciate your reply!
Comment: blast Segmentation fault
by
Tina
• 0
I used 'blastn-short', but it still resulted in a segmentation fault. I downloaded the 'nt_euk' index from NCBI ([enter link description he…
Comment: Help aligning Nanopore Long Read RNA seqs
by
sewaw37819
• 0
> What percentage of your library do you expect to be "long-non-coding transcripts"? A higher percentage than what we are seeing right now…
Comment: Failed to download data from EBI with ascp
by
Michael
54k
Please check my answer. No need to send an email to EBI either.
Answer: Failed to download data from EBI with ascp
by
Michael
54k
**Anonymous Aspera download from ENA works as expected.** Please follow the official documentation https://ena-docs.readthedocs.io/en/late…
Answer: Alignment tool for very short reads (25bp)
by
Jack Tierney
▴ 360
Kudos for writing such a clear and well formatted question. I will give my perspective but don't claim to be an authority. Full disclosure …
Comment: Failed to download data from EBI with ascp
by
ATpoint
83k
Please email EBI and dont post duplicates. Especially not as an answer.
Comment: Help aligning Nanopore Long Read RNA seqs
by
rfran010
▴ 950
What percentage of your library do you expect to be "long-non-coding transcripts"? > (though there IS some coverage, but mostly on whole…
Comment: Overrepresented sequence in reverse read
by
Jaskaran Singh
• 0
Thank You so much....it was really helpful...i was able to remove the poly G with fastp
Comment: Overrepresented sequence in reverse read
by
Jaskaran Singh
• 0
Hi, Thanks, it was helpul information. I used fastp to remove the poly g and the QC seems to be fine now.
Comment: Failed to download data from EBI with ascp
by
ruiyan_hou
• 0
Could anyone who fix this question tell us how to fix it? Thank you!
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