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121,523 results • Page
575 of 2431
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Votes
Replies
1
vote
0
replies
792
views
Beagle 5.2 for imputation, outofmemory or 'bus error' any help?
Imputation
Beagle
3.8 years ago by
zillurbmb51
▴ 10
1
vote
3
replies
1.5k
views
scRNAseq - infer type of single-cell chemistry from fastq
scRNA-seq
single cell
RNA-Seq
updated 3.8 years ago by
bosun1988
• 0 • written 4.4 years ago by
predeus
★ 2.1k
2
votes
1
reply
767
views
how to get exon regions for this gene w/ build 19 coordinates
bed
updated 3.8 years ago by
GenoMax
152k • written 3.8 years ago by
curious
▴ 890
0
votes
0
replies
640
views
Why there are samples with different platforms?
GEO
sample
platform
assay
dataset
updated 3.8 years ago by
Ram
45k • written 3.8 years ago by
Nemo
• 0
11
votes
15
replies
5.4k
views
Output of samtools view, what does the third column actually represent?
samtools
updated 3.8 years ago by
i.sudbery
21k • written 3.8 years ago by
DNAngel
▴ 250
0
votes
2
replies
2.4k
views
How to fasterq-dump 10x genomics snATACseq fastq from SRA
fasterq-dump
snATACseq
scATACseq
sra
10x
updated 3.8 years ago by
swbarnes2
15k • written 3.8 years ago by
rintsen.sherpa
• 0
2
votes
2
replies
4.1k
views
Merging columns from multiple files and adding headers- Linux
linux
merge
join
updated 3.8 years ago by
bioinformatics2020
▴ 840 • written 3.8 years ago by
tenten
• 0
0
votes
2
replies
975
views
Regarding p53 (tumour suppressor gene) mutation and overexpression relation with survival analysis
analysis
survival
3.8 years ago by
jyotsnap
• 0
3
votes
3
replies
2.3k
views
Efficient way for Multiple Sample Mapping with STAR ?
star
3.8 years ago by
rlatjsgns129
▴ 20
3
votes
3
replies
1.0k
views
best tool for the pipeline wrapper
pipeline
updated 3.8 years ago by
Istvan Albert
102k • written 3.8 years ago by
Sara
▴ 280
0
votes
2
replies
1.0k
views
Benchmarking different DESeq2 design models
RNA-sequencing
differential-gene-expression
DESeq2
R
updated 3.8 years ago by
Ram
45k • written 3.8 years ago by
jdhindsa1999
▴ 20
1
vote
1
reply
6.8k
views
Changing colour labels of samples with pheatmap
pheatmap
updated 3.8 years ago by
Papyrus
★ 3.1k • written 3.8 years ago by
mar77
▴ 40
0
votes
5
replies
4.3k
views
Dealing with P value inflation of SNPs identified by qqplot
pooled
SNP
inflation
calling
factor
sequencing
genomic
updated 3.8 years ago by
German.M.Demidov
★ 3.0k • written 3.8 years ago by
stephen.johnson.online
• 0
0
votes
2
replies
923
views
Forum:
How do I use a small font size in questions and answers?
fonts
updated 3.8 years ago by
Istvan Albert
102k • written 3.8 years ago by
Elizabeth
• 0
2
votes
7
replies
3.2k
views
how to increase modules in WGCNA
modules
R
WGCNA
3.8 years ago by
Maryam
• 0
0
votes
0
replies
701
views
News:
Population genomics using ancient DNA data
PopulationGenomics
aDNA
3.8 years ago by
Physalia-courses
★ 2.6k
0
votes
3
replies
1.1k
views
Alignment with inserts and keeping the indexing of ref seq intact
mapping
alignment
sequence-analysis
multiple-sequence-alignment
mutations
3.8 years ago by
iamakhilverma
• 0
3
votes
2
replies
2.0k
views
Volcano plot not centered at origin
R
updated 3.8 years ago by
Ram
45k • written 3.8 years ago by
mm2568
• 0
1
vote
0
replies
628
views
Rare Variant Association analyses
Association
Variant
Cases
Rare
Controls
3.8 years ago by
ClkElf
▴ 50
0
votes
0
replies
628
views
Calculate the Pearson correlation and associated p value for multiple variables
Pearson
correlation
R
updated 3.8 years ago by
rpolicastro
13k • written 3.8 years ago by
Cp.Recker
• 0
0
votes
0
replies
574
views
Coverage drops in fastq alignment against custom Immunoglobulin reference
Alignment
RNASeq
Immunoglobulin
3.8 years ago by
Gama313
▴ 130
0
votes
0
replies
571
views
Calculate nSL
selection
iHS
SNP
3.8 years ago by
Litier
• 0
0
votes
1
reply
2.1k
views
when explained variation per PC is too low while running PCA with SNP data
variation
PCA
SNP
updated 3.8 years ago by
Jean-Karim Heriche
27k • written 3.8 years ago by
?
▴ 60
0
votes
1
reply
1.0k
views
Error: 'calculateQCMetrics' is defunct
scRNA-seq
scater
updated 3.8 years ago by
ATpoint
88k • written 3.8 years ago by
17318598206
▴ 30
0
votes
0
replies
638
views
calculate kappa (transition/transversion) for each tree branch
paml
3.8 years ago by
qqq2395
• 0
0
votes
0
replies
720
views
Mapping digested synthetic oligos back to original sequences.
shortread
digestion
map
3.8 years ago by
Parham
★ 1.6k
3
votes
6
replies
1.9k
views
What is the Rfam version on Biomart Ensembl?
Rfam
version
Biomart
RNAcentral
3.8 years ago by
p.l.rehorst
• 0
0
votes
0
replies
492
views
Method of calculating DAF
snp
3.8 years ago by
Litier
• 0
0
votes
0
replies
636
views
Extracting coordinates of possibly H bonded solvent molecule using BioPython
Biopython
python
PDBParsing
VMD
updated 2.4 years ago by
Ram
45k • written 3.8 years ago by
GS
• 0
2
votes
4
replies
1.8k
views
Bedfile format deeptools
Genome
DNA
Deeptools
ChIP-seq
updated 3.8 years ago by
Devon Ryan
105k • written 3.8 years ago by
Lalita
▴ 10
2
votes
1
reply
1.2k
views
Update SNP map
assembly
update
SNP
map
updated 3.8 years ago by
tothepoint
▴ 940 • written 3.8 years ago by
Rafael
• 0
2
votes
6
replies
2.1k
views
How to construct circRNA-miRNA-mRNA network using cytoscape
circRNA-miRNA-mRNA-networking
microarray-analysis
3.8 years ago by
Babita Pruseth
• 0
2
votes
4
replies
3.4k
views
List of human protein coding genes with given name (known function?)
RNA-Seq
Annotation
Human
updated 3.8 years ago by
Shicheng Guo
★ 9.6k • written 5.9 years ago by
Adrian Pelin
★ 2.7k
2
votes
3
replies
2.7k
views
Is it possible to do mummerplot with reference and query sequences that have many contigs?
alignment
mummer
mummerplot
updated 3.8 years ago by
Philipp Bayer
8.8k • written 3.8 years ago by
jernej.kralj96
▴ 10
0
votes
0
replies
702
views
GPL arguments in getGEO()
GEO
GEOquery
R
3.8 years ago by
Jeffrey3555054
▴ 20
8
votes
10
replies
4.0k
views
6 follow
Renaming files that contain different numbers using rename command-Linux
linux
coding
updated 3.8 years ago by
Joe
22k • written 3.8 years ago by
tenten
• 0
1
vote
1
reply
999
views
Human tissue expression dataset except GTEx
GTEx
updated 3.8 years ago by
hamarillo
▴ 80 • written 3.8 years ago by
Shicheng Guo
★ 9.6k
0
votes
1
reply
826
views
Truncating alignments in a SAM/BAM file
Alignment
SAM
BAM
updated 3.8 years ago by
GenoMax
152k • written 3.8 years ago by
everj
• 0
0
votes
1
reply
855
views
Where is genotype calls in this dataset?
genotype
snps
updated 3.8 years ago by
Lemire
▴ 940 • written 3.8 years ago by
Nemo
• 0
3
votes
3
replies
1.3k
views
Pfam - get only one representative fasta sequence per family
pfam
fasta
protein
updated 3.8 years ago by
Mensur Dlakic
★ 29k • written 3.9 years ago by
Xylanaser
▴ 80
0
votes
1
reply
1.2k
views
split pdb into submodels
pdb
protein
updated 3.8 years ago by
Mensur Dlakic
★ 29k • written 3.8 years ago by
Xylanaser
▴ 80
6
votes
8
replies
3.4k
views
Is it possible to detect of SNVs and InDels in low coverage WGS (5-10X) data?
mutation
low
WGS
lcWGS
pipeline
somatic
coverage
calling
updated 3.8 years ago by
Cyriac Kandoth
6.1k • written 3.8 years ago by
wangziwei0010
▴ 30
1
vote
1
reply
945
views
Data Storage in Plink Format
ID
plink1.9
fam
plink
updated 2.3 years ago by
Ram
45k • written 3.8 years ago by
conor.breen32
• 0
0
votes
0
replies
729
views
How to find out how many reads were used in contig assembly in metaSPADES?
metaspades
3.8 years ago by
DNAngel
▴ 250
1
vote
1
reply
3.1k
views
How do you interpret Tukey's significance when there is no group clustering alone?
Tukey
3.8 years ago by
DNAngel
▴ 250
1
vote
3
replies
1.6k
views
Can someone explain this Trinity/Chrysalis output warning?
Trinty
de-novo
RNAseq
updated 3.8 years ago by
Philipp Bayer
8.8k • written 3.8 years ago by
nina.maryn
▴ 30
0
votes
2
replies
1.4k
views
Interpreting read coverage over gene body plot
chipster
rseqc
illumina
rna-seq
3.8 years ago by
Mikael
• 0
4
votes
4
replies
2.2k
views
Average Amino Acid Identity (AAI) analysis manually
awk
parallel
sed
AAI
BLAST
3.8 years ago by
fec2
▴ 50
2
votes
1
reply
1.1k
views
PACBIO alignment on IGV
Pacbio
IGV
insertions
updated 3.8 years ago by
Istvan Albert
102k • written 3.8 years ago by
jan
▴ 170
0
votes
0
replies
737
views
Error in methyl kit (differential methylation analysis) in R
epeigenetics
methylation
differential
analysis
R
methylkit
DNA
3.8 years ago by
harshraje19
▴ 50
121,523 results • Page
575 of 2431
Recent Votes
Comment: PacBio amplicon reads partially aligned using minimap2 – library or analysis iss
Comment: PacBio amplicon reads partially aligned using minimap2 – library or analysis iss
Answer: NCBI Datasets CLI Question
SNP calling with SAMtools on multiple samples
Comment: Crac: Funny And/Or Weird Names For Bioinformatics Tools
Answer: Crac: Funny And/Or Weird Names For Bioinformatics Tools
Comment: Crac: Funny And/Or Weird Names For Bioinformatics Tools
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Recent Replies
Comment: PacBio amplicon reads partially aligned using minimap2 – library or analysis iss
by
louisflower1999
• 0
Hi GenoMax, Thanks so much for your suggestion! Those amplicons are around 4.4kb. And there should be at least 1kb from the left and 800 b…
Comment: NCBI Datasets CLI Question
by
GenoMax
152k
Wanted to make it a complete answer with an additional command. Moved now.
Comment: NCBI Datasets CLI Question
by
Mensur Dlakic
★ 29k
Maybe make this an answer rather than a comment?
Comment: PCA interpretation
by
swbarnes2
15k
You are trying to PCA these by what, exactly? Binned coverage? Is this really informative?
Answer: NCBI Datasets CLI Question
by
GenoMax
152k
I don't see a way to specify a random number of accessions to download as an option. Perhaps you may want to open an issue and suggest that…
Comment: PacBio amplicon reads partially aligned using minimap2 – library or analysis iss
by
Zev.Kronenberg
12k
@genomax has a good point about trying `pbaa`. The tool will output consensus sequences of the different alleles. You can then use MSA to a…
Answer: Joint Calling for Large Germline WGS Cohort
by
Jeremy Leipzig
23k
Not to get too pedantic but joint genotyping solves a different problem (removing artefactual variants) from producing a population VCF tha…
Answer: Joint Calling for Large Germline WGS Cohort
by
DBScan
▴ 490
Another option would be HAILs VDS Combiner, [https://hail.is/docs/0.2/vds/hail.vds.combiner.VariantDatasetCombiner.html#hail.vds.combiner.V…
Comment: how shoud the phred score be intepreted?
by
QX
▴ 70
thank you for explanation and documentation
Comment: how shoud the phred score be intepreted?
by
GenoMax
152k
Illumina sequencing assumes/generally expects that clusters in a sequencing field have an even distribution of ACTG so for every sequencing…
Comment: how shoud the phred score be intepreted?
by
QX
▴ 70
what do you mean by 'low nucleotide diversity'. If it is low nucleotide diversity, is it supposed to have consistent signals, leading to hi…
Answer: how shoud the phred score be intepreted?
by
GenoMax
152k
> Does the phred-score is affected by the position of the base in all the reads (vertical), or all the bases that belong to a single read (…
Answer: Joint Calling for Large Germline WGS Cohort
by
Pierre Lindenbaum
166k
try glnexus https://github.com/dnanexus-rnd/GLnexus/wiki/Getting-Started
Comment: Inherited pipeline uses RNA-seq variant calls for WASP filtering—shouldn’t it be
by
jonas.andersson
▴ 40
Thank you so much for you answer @iansudbery! Yes I have read it too and I don't think it's totally clear either, so I guess I might not be…
Answer: Inherited pipeline uses RNA-seq variant calls for WASP filtering—shouldn’t it be
by
i.sudbery
21k
Looking through the WASP documentation, and paper, its a little unclear. Clearly what is expected is a set of "known" SNPs. However, I'm no…
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