Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Post does not exist.
Limit : all time
all time
today
this week
this month
this year
117,143 results • Page
1 of 2343
Sort: Rank
Rank
Views
Votes
Replies
0
votes
0
replies
22
views
Subset of Transcripts- How do I proceed with it?
Stringite
Quantification
55 minutes ago by
Varsha
• 0
1
vote
2
replies
3.7k
views
How can run cd-hit-est with a clstr threshold less than 0.8?
cd-hit
cluster
updated 1 hour ago by
Asaf
10k • written 6.3 years ago by
m.koohi.m
▴ 120
0
votes
2
replies
165
views
Tools for chromosomal aneuploidy detection
genetics
aneuploidy
NGS
human
updated 1 hour ago by
a.alnawfal.1992
▴ 260 • written 3 days ago by
adarsh_pp
▴ 40
1
vote
0
replies
34
views
Search within posts based on tags using the Biostars API
api
meta
biostars
1 hour ago by
Bálint
▴ 10
0
votes
0
replies
28
views
PyRMD
Virtual
Screening
learning
cheminformatics
machine
3 hours ago by
s
• 0
3
votes
4
replies
138
views
Invalid CIGAR after using bam clipOverlap
validatesamfile
cigar
bam
clipoverlap
2 hours ago by
MboiTui
▴ 20
801
votes
167
replies
144k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 4 months ago by
Biostar
2.8k • written 7.5 years ago by
Istvan Albert
100k
0
votes
0
replies
49
views
Determining rs ids from SNP HGV base ID?
base
RsID
SNP
ID
HGV
10 hours ago by
Katherine
• 0
0
votes
2
replies
177
views
How to add Ensembl ids after Pseudobulk analysis by DESeq2
Seurat
Pseudobulk
single-cell
DESeq2
scRNA
2 hours ago by
Sara
▴ 30
0
votes
1
reply
125
views
Facing problem with single cell rna seq annotation using singleR
annotation
singleR
scRNAseq
updated 11 hours ago by
jared.andrews07
★ 17k • written 20 hours ago by
sc_analysis
• 0
0
votes
0
replies
46
views
Imputed data doesn't match directly genotyped APOE genotype
APOE
directgenotyping
imputation
11 hours ago by
kl
▴ 10
0
votes
0
replies
65
views
Tubemap multiseq
vg
17 hours ago by
karciharun42
• 0
5
votes
3
replies
5.8k
views
Attempt to set 'rownames' on an object with no dimensions
R
microbiome
spieceasi
updated 17 hours ago by
GenoMax
142k • written 3.6 years ago by
ks.sholohova
▴ 30
0
votes
0
replies
80
views
News:
Online course: Population Genomic Inference from Low-Coverage Whole-Genome Sequencing Data
PopulationGenomics
GenotypeLikelihoods
Low-Coverage
ANGSD
1 day ago by
carlopecoraro2
★ 2.5k
0
votes
1
reply
129
views
How to decrease the motif score?
scores
Motif
updated 2 hours ago by
ATpoint
82k • written 1 day ago by
Hadia
• 0
0
votes
0
replies
103
views
immuCell Abundance infiltrate
ImmuCell
Abundance
infiltrate
updated 1 day ago by
Pierre Lindenbaum
162k • written 1 day ago by
elbakri.fatimazahrae
• 0
1
vote
2
replies
200
views
Setting a threshold on gene expression for subsetting (scRNA-seq)
scRNA-seq
updated 17 hours ago by
LauferVA
4.2k • written 2 days ago by
carolofharvest
▴ 40
0
votes
0
replies
100
views
MaxEntScan for rMATS results
MaxEntScan
rMATS
1 day ago by
adi.gershon1
• 0
0
votes
0
replies
316
views
ERROR with Maker annotation: The assumed RepeatMasker installation directory /opt/home/sony/miniconda3/envs/MAKER2/share/RepeatMasker does not ap…
RepeatMasker.
MAKER.
annotation.
2 days ago by
Sony
▴ 10
0
votes
0
replies
111
views
Trouble with PLINK's logistic regression analysis and covariatesTrouble with PLINK's logistic regression analysis and covariates
PLINK
GWAS
SNP
2 days ago by
F110152169
• 0
4
votes
2
replies
208
views
Number of non-ATCG nucleotides replaced by Salmon
rna-seq
stringtie
indexing
salmon
updated 2 days ago by
Rob
6.6k • written 2 days ago by
Tonya S.
▴ 10
0
votes
3
replies
339
views
Tool:
Introducing NovaDemux, a Sequence Demultiplexer that Increases Yield and Salvages Failed Runs
NovaDemux
NovaSeq
Illumina
Demultiplexing
Barcode
2 days ago by
Brian Bushnell
20k
2
votes
11
replies
740
views
ComplexHeatmap - How to change fontsize of rowAnnotation
r
ComplexHeatmap
updated 19 hours ago by
Ram
44k • written 9 days ago by
hannes.bongartz
• 0
0
votes
1
reply
158
views
perl Error - needLargeMem Bigwig
Bigwig
perl
mitosalt
updated 2 hours ago by
b.contreras.moreira
▴ 200 • written 2 days ago by
marco.barr
▴ 130
0
votes
0
replies
124
views
Duplicates of the same gene in Orthogroup sequence files
orthofinder
2 days ago by
niconps14
• 0
3
votes
5
replies
346
views
How to scrape BioMart data from https://sorfs.ugent.be/ website
BioMart
updated 22 hours ago by
Pierre Lindenbaum
162k • written 3 days ago by
QX
• 0
2
votes
6
replies
388
views
Harmony integration group.by.var parameter
single-cell
harmony
updated 11 hours ago by
jared.andrews07
★ 17k • written 2 days ago by
Picasa
▴ 640
2
votes
1
reply
180
views
Why some SNP's allele frequencies in gnomAD are so different between v2 and v4?
AF
gnomAD
SNP
updated 2 days ago by
Jeremy Leipzig
22k • written 2 days ago by
liaotsungjen
▴ 10
0
votes
0
replies
117
views
Exon Annotation failed using homer annotatePeaks.pl
homer
alternative-splicing
annotatePeaks
updated 2 days ago by
Ram
44k • written 2 days ago by
Alessia
• 0
1
vote
3
replies
268
views
How to calculate cell type frequency between two groups in single cell data
Seurat
single-cell
sc-RNA
cell-type
updated 1 day ago by
Bastien Hervé
5.3k • written 2 days ago by
Sara
▴ 30
0
votes
1
reply
159
views
Mutation counts corrected by number of samples
mutations
WES
genomics
WGS
updated 2 days ago by
Ram
44k • written 2 days ago by
garcesj
▴ 50
0
votes
1
reply
3.0k
views
bfctools merge [E::hts_open_format] Failed to open file
SNP
updated 2 days ago by
Ram
44k • written 3.6 years ago by
andemexoax
• 0
0
votes
1
reply
379
views
LDhat lookup table
LDhat
updated 2 days ago by
Ram
44k • written 11 months ago by
Matteo
▴ 10
0
votes
0
replies
133
views
BAM to Bedgraph creating an empty file
Bisulfite-seq
bismark
2 days ago by
irebekah.c
• 0
0
votes
0
replies
123
views
Can you use a polygenic risk score for a trait (e.g., BMI) in association analyses when it doesn't associate with BMI in the sample it is used?
polygenic-risk-score
imputation
updated 2 days ago by
Ram
44k • written 2 days ago by
kl
▴ 10
0
votes
5
replies
240
views
Bowtie 1.3.1 alignment error as array 21720,23124 produces sam bam files
Bowtie
2 days ago by
Deepthi
• 0
0
votes
1
reply
242
views
Annotating single cell data automatically
single-cell
updated 2 days ago by
Ram
44k • written 5 weeks ago by
Gerard
• 0
1
vote
4
replies
304
views
Single cell analysis: Unable to subset cells in seurat object using desired nFeature_RNA cut off
single-cell
2 days ago by
sc_analysis
• 0
0
votes
1
reply
191
views
Overlapping clusters for different biological conditions: Seurat, UMAP
Seurat
scRNA-seq
UMAP
updated 2 days ago by
Bastien Hervé
5.3k • written 3 days ago by
bio_info
▴ 20
3
votes
4
replies
474
views
Single-cell ambient RNA correction: SoupX vs decontX contamination fraction
single-cell
ambient-RNA
updated 2 days ago by
fracarb8
★ 1.7k • written 10 days ago by
txema.heredia
▴ 130
0
votes
0
replies
148
views
R code for KEGG pathways output
KEGGREST
dplyr
KEGG
R
3 days ago by
doramora
▴ 10
0
votes
0
replies
122
views
finding highly divergent orthologs using exonerate
ortholog
Exonerate
evolution
3 days ago by
Emily
▴ 20
2
votes
3
replies
251
views
How to calculate nucleotide diversity (mtDNA, PacBio_data), Suggest me some tools
NucleotideDiversity
mtDNA
PacBio
3 days ago by
hashim.rana11
▴ 20
0
votes
1
reply
178
views
Does prokka work the same on forward and reverse complementary reads?
Prokka
updated 3 days ago by
Pierre Lindenbaum
162k • written 3 days ago by
Maksim
• 0
1
vote
2
replies
270
views
Trouble with PLINK's logistic regression analysis and covariatesTrouble with PLINK's logistic regression analysis and covariates
PLINK
GWAS
SNP
2 days ago by
F110152169
• 0
8
votes
9
replies
3.1k
views
7 follow
Venter Genome Vcf
vcf
updated 3 days ago by
ericrkofman
▴ 20 • written 10.1 years ago by
win
▴ 980
0
votes
0
replies
153
views
16S analysis for specie of bacteria identification
Species-identification
16S
Kraken2
updated 3 days ago by
Ram
44k • written 3 days ago by
Christopher
▴ 10
4
votes
1
reply
228
views
PCA plot no distinct cluster. Only p < 0.05 indicates significant DEGs, while adj p < 0.05 does not.
RNA-seq
padj
p-value
updated 3 days ago by
swbarnes2
14k • written 3 days ago by
May Ling
• 0
0
votes
0
replies
133
views
abundance_estimates_to_matrix.pl in Trinity is not generating any matrices / output
trinity
updated 3 days ago by
GenoMax
142k • written 3 days ago by
jway
• 0
0
votes
3
replies
352
views
What is a good way to do gene differentials in single cell data where one group is small 90 cells and the other group 30,000 cells or 2000 cells.
cell
RNA-Seq
single
updated 3 days ago by
ATpoint
82k • written 3 days ago by
shahzaibali
• 0
117,143 results • Page
1 of 2343
Recent Votes
C: The Biostar Handbook. A bioinformatics e-book for beginners.
Answer: Error with BiocParallel. No barcodes files found
Error with BiocParallel. No barcodes files found
Comment: How do we do quantification using stringtie merge option for all the merged samp
Answer: bcftools view error: the tag "mis" is not defined in the VCF header
Python script to query GeneCards to get EntrezID, symbol from Ensembl geneID
Search within posts based on tags using the Biostars API
Recent Locations •
All
Czech Republic,
1 minute ago
Italy,
2 minutes ago
Hong Kong,
2 minutes ago
Hong Kong,
2 minutes ago
UK,
3 minutes ago
London, United Kingdom,
4 minutes ago
Netherlands,
5 minutes ago
Recent Awards •
All
Popular Question
to
jjmmii
• 0
Scholar
to
Asaf
10k
Voter
to
wangh920
▴ 10
Popular Question
to
User000
▴ 700
Popular Question
to
A_heath
▴ 160
Popular Question
to
najibveto
▴ 110
Scholar
to
Zeng Jingyu
▴ 80
Recent Replies
Comment: How do we do quantification using stringtie merge option for all the merged samp
by
Varsha
• 0
Thank you for the detailed explanation.
Answer: How can run cd-hit-est with a clstr threshold less than 0.8?
by
Asaf
10k
For future reference The 0.8 identity threshold for EST (nucleotides) is hardcoded. However, there's an option to use `-D` (distance) in…
Answer: Tools for chromosomal aneuploidy detection
by
a.alnawfal.1992
▴ 260
As far as I know, all the tools utilised control group to compute the z score for chromosomal aneuploidy. but is your goal detect chromosom…
Comment: How to add Ensembl ids after Pseudobulk analysis by DESeq2
by
Sara
▴ 30
I used Seurat, and in Seurat, I have gene names (which some are with gene-symbols and some with ENSG ids). Then I did Pseudobulk. how can …
Comment: How to decrease the motif score?
by
ATpoint
82k
So you scan the entire genome and got nothing significant? Is that the issue?
Comment: perl Error - needLargeMem Bigwig
by
b.contreras.moreira
▴ 200
A quick search suggests the problem is your empty input file? See <https://biostar.galaxyproject.org/p/6794>
Comment: Invalid CIGAR after using bam clipOverlap
by
MboiTui
▴ 20
ooh good pickup. Might not be the problem but definitely not helping. Will re-run with streamlined workflow and with double quotes and see …
Comment: Invalid CIGAR after using bam clipOverlap
by
Pierre Lindenbaum
162k
may be not the problem but why this ` RG:Z:H7J22DRX3.${lane}-1C23074F ` in the RG. you're using single quotes with bwa-mem and it won't …
Comment: Invalid CIGAR after using bam clipOverlap
by
MboiTui
▴ 20
Hello Pierre, Thanks for your reply and insight. with samtools view | grep using the erroneous CIGAR string I get: A00152:841:H7J22D…
Comment: Invalid CIGAR after using bam clipOverlap
by
Pierre Lindenbaum
162k
please, show us the following output: samtools view "${Ind1}_sorted_dup.bam" | grep -F "A00152:841:H7J22DRX3:2:1112:5267:11397" Furth…
Comment: Failed to download data from EBI with ascp
by
孝中
• 0
Hi, have you found the solution to this problem?
Comment: samtools write-index
by
R.L.
• 0
Oh God, thank you so much for helping me in both platforms! I am gonna upgrade my samtools to stay tracked with you and your team! Thank yo…
Answer: How to add Ensembl ids after Pseudobulk analysis by DESeq2
by
jared.andrews07
★ 17k
Go back to your original counts matrix or input data and assign consistent IDs during its generation.
Answer: Facing problem with single cell rna seq annotation using singleR
by
jared.andrews07
★ 17k
A few things. >(1)What did i do wrong here? SingleR will annotate each cell with whatever it's most similar to in the reference datas…
Comment: Harmony integration group.by.var parameter
by
jared.andrews07
★ 17k
Couldn't tell you, I avoid Seurat like the plague.
Traffic: 2269 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6