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1,000 results • Page
1 of 20
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Views
Votes
Replies
5
votes
3
replies
237
views
Forum:
Ideal PC configurations and operating system for bioinformatics laboratory
PC
updated 3 days ago by
GenoMax
141k • written 3 days ago by
Estevão
• 0
0
votes
0
replies
162
views
Forum:
Improvement on automatic annotation of single-cell RNA-seq data from the literature using LLM
annotation
scRNA
llm
4 days ago by
yxwucq
• 0
4
votes
2
replies
283
views
Forum:
Bioinformatics: Where do I start
Sequencing
updated 10 days ago by
Ram
43k • written 10 days ago by
Adi
▴ 10
7
votes
7
replies
1.2k
views
Forum:
Bioinformatician who was retaliated against after reporting discriminatory harassment - Please help me navigate getting out of the situation I'm in
Career
advice
updated 11 days ago by
Dunois
★ 2.5k • written 12 days ago by
AdviceSeeker
• 0
1
vote
2
replies
339
views
Forum:
VCF bulk handling
bcftools
SnpEff
WGS
VEP
VCF
9 days ago by
Javier
• 0
16
votes
10
replies
1.0k
views
6 follow
Forum:
F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 28 days ago by
Madelaine Gogol
5.3k • written 29 days ago by
Ann
★ 2.4k
1
vote
1
reply
219
views
Forum:
Can I use this community for software development?
software-development
updated 4 weeks ago by
Ram
43k • written 4 weeks ago by
avpuzanov
• 0
5
votes
2
replies
384
views
Forum:
Can questions and answers on biostars be asked only in English? Can't I use Russian for example?
language
updated 4 weeks ago by
Michael
54k • written 4 weeks ago by
avpuzanov
• 0
0
votes
0
replies
172
views
Forum:
Field of Study in Computational Biology
Genomics
Computational-Biology
PhD
updated 5 weeks ago by
Ram
43k • written 5 weeks ago by
Mohammed
• 0
1
vote
3
replies
474
views
Forum:
Development plan for a bioinformatics database developer/coder
developer
6 weeks ago by
pixie@bioinfo
★ 1.5k
7
votes
8
replies
673
views
Forum:
Bioinformatics career transition
career
forum
updated 6 weeks ago by
jared.andrews07
★ 16k • written 6 weeks ago by
jevill17
▴ 20
4
votes
2
replies
474
views
Forum:
Exploring Amino Acid Patterns in Proteins Through N-gram Analysis
protein
CAFA5
7 weeks ago by
Anatoly A.
▴ 10
6
votes
6
replies
764
views
Forum:
You have 3 days of zero obligations and expansive resources. What are you building?
hypothetical
thought-experiment
7 weeks ago by
jared.andrews07
★ 16k
0
votes
0
replies
161
views
Forum:
What other data types and pipelines you would like to see from the Genomics Data Commons (GDC)
gdc
tcga
genomics-data-commons
updated 7 weeks ago by
Ram
43k • written 7 weeks ago by
Zhenyu Zhang
★ 1.2k
0
votes
2
replies
368
views
Forum:
What's the state of the art in bacterial whole genome alignment?
whole
genome
alignment
mauve
7 weeks ago by
Dave Carlson
★ 1.7k
31
votes
12
replies
950
views
7 follow
Forum:
Nextflow and self-made pipelines opinion
nextflow
pipeline
data-analysis
8 weeks ago by
karlaarz
▴ 90
5
votes
4
replies
554
views
Forum:
Would reaching out to local labs and startups help with my career transition?
career
volunteering
updated 9 weeks ago by
GenoMax
141k • written 9 weeks ago by
Mango
▴ 10
1
vote
2
replies
368
views
Forum:
Exploring Career Reentry: Challenges and opportunities for Women After Maternity and Childcare Breaks
Maternity
Career-Gap
career-reentry
updated 9 weeks ago by
ATpoint
82k • written 9 weeks ago by
ashaneev07
▴ 20
8
votes
7
replies
790
views
6 follow
Forum:
Tower Server configuration for NGS data analysis
NGS
updated 9 weeks ago by
emmanouil.a
▴ 120 • written 12 weeks ago by
harasharan
• 0
1
vote
0
replies
277
views
Forum:
list of bioinformatics tools in cancer genomics
mutations
CNA
cancer
genomics
LOH
updated 9 weeks ago by
Pierre Lindenbaum
161k • written 9 weeks ago by
Bogdan
★ 1.4k
4
votes
2
replies
372
views
Forum:
bulk seq vs. sc-seq
scATAC-seq
ATAC-seq
scRNAseq
RNAseq
updated 11 weeks ago by
dsull
★ 5.8k • written 11 weeks ago by
SebKittelmann
▴ 10
8
votes
6
replies
2.8k
views
Forum:
Demonstrating Exponential Growth In Pre-School
education
written 10.2 years ago by
Asaf
10k
0
votes
0
replies
200
views
Forum:
tutorial for carrier screening analyses?
carrier-screening
updated 12 weeks ago by
Ram
43k • written 12 weeks ago by
Akos
▴ 20
2
votes
3
replies
434
views
Forum:
My Project
Project
CLI
MD-Simulations
Molecular-docking
3 months ago by
Ajay A Shettigar
• 0
17
votes
11
replies
981
views
Forum:
How to overcome the imposter syndrome in bioinformatics?
advice
3 months ago by
sil_bioinfo
▴ 40
10
votes
7
replies
2.2k
views
Forum:
How to learn bioinformatics?
learning
updated 3 months ago by
Samuel Lampa
★ 1.3k • written 23 months ago by
sakichwan
▴ 20
26
votes
27
replies
2.0k
views
10 follow
Forum:
Determining computer time required
computer
hpc
time
updated 3 months ago by
dsull
★ 5.8k • written 3 months ago by
Rozita
▴ 40
7
votes
6
replies
730
views
Forum:
where to work as a bioinformatician (NGS/IA)
employment
omics
ia
ngs
updated 3 months ago by
Ram
43k • written 3 months ago by
Petur
▴ 20
21
votes
12
replies
3.5k
views
Forum:
How to Use Biostars Part-3: Formatting Text and Using GitHub Gists
meta
how-to
documentation
3 months ago by
Ram
43k
2
votes
1
reply
420
views
Forum:
Cataloguing available spatial transcriptomics tools, then linking them together into a workflow?
cytassist
transcriptomics
nanostring
xenium
visium
3 months ago by
LauferVA
4.2k
49
votes
20
replies
2.0k
views
10 follow
Forum:
First experiences matter
first-time
tolerance
updated 3 months ago by
Michael Love
★ 2.6k • written 3 months ago by
Mensur Dlakic
★ 27k
4
votes
3
replies
759
views
Forum:
How to get started in bioinformatics
students
updated 3 months ago by
Ram
43k • written 3 months ago by
Soidihane
▴ 10
2
votes
2
replies
581
views
Forum:
Do batches contain biological variations in omics data (RNAseq or scRNAseq)?
combat
single-cell
omics
batch
rnaseq
updated 3 months ago by
Ram
43k • written 4 months ago by
cwwong13
▴ 40
0
votes
2
replies
3.2k
views
Forum:
I’m considering a career transition from software engineering to bioinformatics. Is it a good fit for me?
career
updated 4 months ago by
GenoMax
141k • written 4 months ago by
Mango
▴ 10
5
votes
3
replies
604
views
Forum:
Binning the rare biosphere
metagenomics
soil
assembly
techniques
binning
updated 4 months ago by
Mensur Dlakic
★ 27k • written 4 months ago by
Konstantin
▴ 20
6
votes
2
replies
660
views
Forum:
how to start Bioinformatics?
basics
updated 4 months ago by
Mensur Dlakic
★ 27k • written 4 months ago by
sajibsuvo70
• 0
19
votes
5
replies
1.1k
views
6 follow
Forum:
What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2023?
genomics
snps
survey
papers
4 months ago by
Raony Guimarães
★ 1.4k
4
votes
6
replies
926
views
6 follow
Forum:
Bioinformatics: what do you think of it in the next 20 years?
future
updated 4 months ago by
Ram
43k • written 4 months ago by
Daniel
• 0
1
vote
1
reply
486
views
Forum:
want to work in open source project/project in bioinformatics
project
updated 4 months ago by
Ram
43k • written 5 months ago by
Afeef
• 0
3
votes
5
replies
1.0k
views
Forum:
Current Trends in Bioinformatics
structural-bioinformatics
updated 4 months ago by
Ram
43k • written 5 months ago by
Paulo
• 0
21
votes
11
replies
2.5k
views
6 follow
Forum:
Rust bioinformatics projects ideas
projects
scRNA-seq
RNA-seq
Rust
updated 5 months ago by
Jeremy Leipzig
22k • written 13 months ago by
bompipi95
▴ 150
176
votes
46
replies
19k
views
26 follow
Forum:
Crac: Funny And/Or Weird Names For Bioinformatics Tools
humor
5 months ago by
Istvan Albert
100k
3
votes
2
replies
621
views
Forum:
RNA-Seq data analysis - question
Python
RNA-Seq
R
updated 5 months ago by
fturner
▴ 10 • written 5 months ago by
sil_bioinfo
▴ 40
0
votes
0
replies
369
views
Forum:
How to learn bioinformatics online?
self-education
education
learning
updated 5 months ago by
Ram
43k • written 5 months ago by
Brian Rey | BioLearn
• 0
6
votes
5
replies
2.3k
views
Forum:
Maintaning internal or private installation of Biostar or a similar Q&A site
Biostars
6 months ago by
tshtatland
▴ 190
7
votes
9
replies
1.4k
views
6 follow
Forum:
Mentor for a beginner bioinformatics student
project
mentor
6 months ago by
UT_FAN
▴ 40
0
votes
0
replies
450
views
Forum:
Steps after doublet detection in single cell data
scRNA
doublet
6 months ago by
ishwarvh
• 0
0
votes
0
replies
354
views
Forum:
Automated Checks And QA
retrospective
6 months ago by
Sasha
▴ 840
7
votes
13
replies
1.4k
views
Forum:
How do you validate and verify your pipeline's software updates?
Validation
Verification
Quality-Assurance
updated 6 months ago by
Ram
43k • written 6 months ago by
kieran
▴ 10
21
votes
7
replies
11k
views
Forum:
Bioinformatics short paper journal resource [software/webapps/database]
journal
software
webapp
updated 6 months ago by
Gennady Khvorykh
▴ 100 • written 8.1 years ago by
Sukhi Singh
11k
1,000 results • Page
1 of 20
Recent Votes
A: Blast Settings For Short Sequences
A: Blast Settings For Short Sequences
Comment: Heatmap and rna-seq
Answer: Heatmap and rna-seq
Comment: Heatmap and rna-seq
A: should FASTA files be sorted before indexed with SAMtools?
Answer: A faidx-indexed FASTA format file or a FASTA format file
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Recent Awards •
All
Scholar
to
Alex Reynolds
35k
Popular Question
to
chaco001
▴ 40
Commentator
to
GenoMax
141k
Popular Question
to
rheab1230
▴ 140
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BioinfGuru
★ 1.7k
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bk11
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vinayjrao
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Recent Replies
Answer: Limma Analysis Agilent Microarray Data (GPL1708)
by
Gordon Smyth
★ 7.0k
There is an Agilent array case study in the limma User's Guide, which you might find helpful and somewhat simpler. I don't recommend col…
Answer: Correct way to compare multiple treaments between RNA-Seq samples using edgeR?
by
Gordon Smyth
★ 7.0k
The comparison you've done seems exactly right to test whether treatment 2 modifies or enhances the effect of treatment 1. Have you exam…
Answer: How to use limma to find differentially expressed genes in response to a continu
by
Gordon Smyth
★ 7.0k
There's no need for form a contrast because the score variable is already in the model. A continuous variable is its own contrast. Just run…
Comment: Create a new bed file with all pairwise combinations between two other bed files
by
J
▴ 10
Oh wow, bedops closest-features, how did I overlook that!! I didn't know about it; thank you! Your awk script is beautiful. This worked per…
Comment: Create a new bed file with all pairwise combinations between two other bed files
by
J
▴ 10
Thank you Pierre! I'll try this out with my full data set.
Answer: DNA methylation preprocessing
by
cao510927
▴ 30
I happend to see one paper doing the similar thing, share it with you guys, "A systematic evaluation of normalization methods and probe rep…
Answer: Create a new bed file with all pairwise combinations between two other bed files
by
Alex Reynolds
35k
I think Pierre's answer might be correct for your test input, but it doesn't account for the start position of the reference element and so…
Comment: Highest variable features in single cell data
by
bk11
★ 2.4k
You need to be clear about how `Seurat` defines highly variable genes here. Highly variable genes are the genes that have very high express…
Comment: Heatmap and rna-seq
by
GenoMax
141k
Please include a link when you are recommending a tool. There can be programs with similar names.
Answer: Heatmap and rna-seq
by
dsull
★ 5.8k
Check out clustergrammer from Avi Ma'ayan's lab.
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix
by
Ram
43k
Simple: ```r data <- lapply(files, read_tsv) data <- Reduce(merge, data) # or Reduce(full_join, data) ``` <a href="" title="Text added bec…
Answer: Source other conda environments in a nextflow pipeline when nextflow itself is i
by
ATpoint
82k
You can make each process use a dedicated environment, see https://www.nextflow.io/docs/latest/conda.html#use-existing-conda-environments …
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix
by
BioinfGuru
★ 1.7k
Hi, Just thought I'd share this code snippet here for when each file contains multiple samples. I tried using lapply (as suggested by Ram)…
Comment: Low mapping rate with Salmon
by
i.sudbery
19k
Take your STAR alignment and sum all the counts for each gene. You can either do this by providing an annotation to STAR, or by running fea…
Comment: How to convert plink files to Hapmap Format
by
bk11
★ 2.4k
If you run your data with plink 2.0, you will have ERRCODE column in your result file showing the reason behind "NA" p-value. https://www.b…
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