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184 results • Page
1 of 4
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Votes
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76
votes
72
replies
19k
views
19 follow
Tutorial:
Clustering of DAVID gene enrichment results from gene expression studies
enrichment
david
3 days ago by
Kevin Blighe
87k
25
votes
11
replies
3.7k
views
6 follow
Forum:
Site Use Guidelines
meta
biostars
Guidelines
updated 4 days ago by
Ram
43k • written 14.6 years ago by
Istvan Albert
100k
19
votes
11
replies
14k
views
10 follow
How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
utr
updated 1 day ago by
cmdcolin
★ 3.8k • written 10.8 years ago by
J.F.Jiang
▴ 920
14
votes
2
replies
7.0k
views
Trouble with Pilon installation
polishing
pilon
installation
updated 3 days ago by
Lissa Cruz Saavedra
• 0 • written 7.3 years ago by
Rox
★ 1.4k
10
votes
6
replies
9.9k
views
6 follow
CDS vs cDNA vs transcript for mapping RNA-Seq reads
Assembly
rna-seq
alignment
updated 2 days ago by
Antonio R. Franco
★ 5.1k • written 6.0 years ago by
williamsbrian5064
▴ 510
8
votes
16
replies
858
views
How to convert plink files to Hapmap Format
GWAS
Plink
updated 1 day ago by
bk11
★ 2.4k • written 7 weeks ago by
Sofia
• 0
8
votes
5
replies
5.3k
views
ISCN annotation for SV/CN VCF files
SV
written 7 months ago by
a.beggs
▴ 60
8
votes
8
replies
572
views
PCA plot (Suggestions Needed)
DESeq2
PCAplot
updated 3 days ago by
swbarnes2
14k • written 4 days ago by
Aaliya
▴ 10
7
votes
7
replies
5.9k
views
6 follow
How to know synonymous and non-synonymous SNPs from transcriptome sequencing
RNA-Seq
SNP
updated 2 days ago by
rohitsatyam102
▴ 850 • written 6.1 years ago by
smallfish
▴ 10
7
votes
7
replies
546
views
NGS forensics: how to know if data is fabricated
fastq
STAR
NGS
Illumina
6 hours ago by
noodle
▴ 520
7
votes
6
replies
485
views
Filtering sam or bam file with maximum matching region
crisper-edited
alignment
match
6 days ago by
analyst
▴ 30
7
votes
2
replies
271
views
Gene set enrichment analysis differences between 2020 and 2024
Gene-ontology
updated 4 days ago by
geneontologyhelp
▴ 390 • written 5 days ago by
catherine.teyssier
• 0
6
votes
3
replies
185
views
Interpretting IGV output
IGV
updated 6 days ago by
Carlo Yague
8.7k • written 6 days ago by
analyst
▴ 30
5
votes
4
replies
639
views
How to compare the quality of assemblies
nextdenovo
assembly
hifiasm
pacbio
4 days ago by
kirillkirilenko
▴ 40
5
votes
3
replies
250
views
Forum:
Ideal PC configurations and operating system for bioinformatics laboratory
PC
updated 4 days ago by
GenoMax
141k • written 4 days ago by
Estevão
• 0
4
votes
4
replies
378
views
Tutorial:
how to combine multiple RNAseq count files into a single dataframe in R and unix
Unix
RNAseq
R
updated 20 hours ago by
BioinfGuru
★ 1.7k • written 3 days ago by
Ming Tommy Tang
★ 3.9k
4
votes
11
replies
630
views
Redirection of Duplicate PMIDs
pubmed
pmid
updated 4 days ago by
LauferVA
4.2k • written 5 days ago by
dominickd
• 0
4
votes
7
replies
450
views
To get p-values for the TPM
P-value
TPM
RSEM
updated 3 days ago by
dsull
★ 5.9k • written 4 days ago by
VITALA
• 0
4
votes
8
replies
524
views
Create a new bed file with all pairwise combinations between two other bed files, based on bp distance
SNPs
BED
eqtl
bedtools
updated 1 day ago by
Alex Reynolds
35k • written 2 days ago by
J
▴ 10
4
votes
2
replies
375
views
Missing protein (VEGF-A) in String db
VGFA
stringdb
updated 2 days ago by
shalespringer
• 0 • written 11 weeks ago by
Phenylananin
▴ 20
4
votes
7
replies
580
views
7 follow
Heatmap and rna-seq
RNA-Seq
Heatmap
updated 1 day ago by
dsull
★ 5.9k • written 5 days ago by
qudrat.nii
▴ 10
3
votes
2
replies
277
views
p-value and Boneferroni adjusted p-value the same in R
Bonferroni
R
Statistics
p-value
Fisher
5 days ago by
kristiewyleung
• 0
3
votes
3
replies
229
views
Getting the nucleotide sequence of proteins, having their UniProt IDs
Nucleotide
Genomic
Sequence
Uniprot
Proteins
updated 5 days ago by
GenoMax
141k • written 5 days ago by
Mariana
▴ 10
3
votes
3
replies
224
views
genome assembly records not present in assembly_summary.txt
ncbi
bacteria
assembly
updated 1 day ago by
GenoMax
141k • written 2 days ago by
sapuizait
▴ 10
3
votes
5
replies
554
views
SLURM submission problem: Indexing the Reference Genome is not done in STAR
align
RNA-Seq
linux
STAR
6 days ago by
n_navy
• 0
3
votes
3
replies
722
views
KissDE and batch effect
kissDE
kissplice
5 days ago by
david.b.rombaut
▴ 10
3
votes
5
replies
278
views
Per base sequence content error in RNAseq analysis
RNA-seq
analysis
updated 5 days ago by
GenoMax
141k • written 6 days ago by
ahmad.sajad4541
• 0
3
votes
9
replies
454
views
Is it Acceptable to Have Uniform Quality Scores in a FASTQ File?
FASTQ
updated 3 days ago by
Istvan Albert
100k • written 5 days ago by
Κοσμάς
• 0
3
votes
6
replies
659
views
Ciriquant not configuring hisat2 indexed files
ciriquant
6 days ago by
Atul K.
• 0
3
votes
4
replies
303
views
Why gatk VariantAnnotator required bam and coverage files
gatk
VariantAnnotator
2 days ago by
QX
• 0
3
votes
3
replies
203
views
Genbank vs SRA
ncbi
genbank
genomics
sra
updated 5 days ago by
Mensur Dlakic
★ 27k • written 5 days ago by
c_u
▴ 520
3
votes
3
replies
243
views
Sequence read length shorter than flow cell specification
illumina
NGS
sequencing
updated 2 days ago by
swbarnes2
14k • written 2 days ago by
M
• 0
3
votes
6
replies
1.9k
views
7 follow
DNA methylation preprocessing
SWAN
DNA
methylation
Funnorm
updated 1 day ago by
Ram
43k • written 2.4 years ago by
SYOSY
▴ 10
3
votes
0
replies
122
views
News:
Interested in Spatial Omics?
Bioconductor
Spatial-Omics
Spatial-Transcriptomics
updated 4 days ago by
Ram
43k • written 4 days ago by
carlopecoraro2
★ 2.5k
3
votes
0
replies
148
views
News:
Webinar “Learning Single-cell Atlases” with Dr. Fabian Theis, author of Scanpy on May 8
Scanpy
Generative-AI
scRNA
Machine-Learning
updated 1 day ago by
Ram
43k • written 2 days ago by
Claire Watson
▴ 60
3
votes
1
reply
224
views
Seurat merge and batch correction
Seurat
updated 4 days ago by
Ram
43k • written 5 days ago by
sooni
▴ 20
3
votes
4
replies
667
views
Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (without Blat)
mapping
5 days ago by
rahu
• 0
3
votes
3
replies
2.3k
views
How to make a TSS enrichment plot in ATAC-seq analysis?
ATAC
updated 2 days ago by
sp
• 0 • written 22 months ago by
Dan
▴ 180
2
votes
4
replies
2.4k
views
How could I generate a gi_taxid_nucl.dmp file similar to the one previously hosted by NCBI?
ncbi
metagenomics
centrifuge
gi_taxid_nucl.dmp
taxonomy
updated 3 days ago by
ZhangYuanfeng
• 0 • written 2.4 years ago by
Frazier Baker
• 0
2
votes
3
replies
954
views
Method to detect genome doubling
CNV
genome doubling
updated 5 days ago by
LauferVA
4.2k • written 4.5 years ago by
CY
▴ 750
2
votes
5
replies
300
views
How to trim transcripts using information from NCBI contamination screen report
RNAseq
assembly
transcriptome
contamination
2 days ago by
Lada
▴ 30
2
votes
2
replies
255
views
what is the purpose of indexing the reference genome (Kallisto)
indexing
Kallisto
updated 1 day ago by
dsull
★ 5.9k • written 2 days ago by
Aaliya
▴ 10
2
votes
3
replies
330
views
How to establish haplotype-specific gene expression levels
RNA-seq
Haplotype
updated 1 day ago by
dsull
★ 5.9k • written 18 days ago by
javanokendo
▴ 60
2
votes
3
replies
207
views
from row count to tpm
tpm
row-count
normalization
5 days ago by
michelafrancesconi9
▴ 20
2
votes
4
replies
320
views
What is the real meaning of relative enrichment/peak height of ChIP-seq tracks?
ChIP-seq
4 days ago by
HyperEvo
• 0
2
votes
6
replies
3.7k
views
Treatment VS Control in Single Cell RNAseq analysis
Single Cell
RNAseq
DE Analysis
Treatment
Control
updated 2 days ago by
ATpoint
82k • written 3.9 years ago by
grayapply2009
▴ 280
2
votes
2
replies
215
views
HaplotypeCaller - only SNPs
HaplotypeCaller
GATK
updated 4 days ago by
analyst
▴ 30 • written 4 days ago by
lorena9132
• 0
2
votes
9
replies
846
views
TCGA2STAT Error: Firehose connection
FIREHOSE
TCGA
updated 4 days ago by
LauferVA
4.2k • written 6 months ago by
Gnana
• 0
2
votes
4
replies
309
views
Fetch table from clinvar database according to a list of rsid
python
clinvar
perl
updated 2 days ago by
Ram
43k • written 3 days ago by
ashaneev07
▴ 20
2
votes
4
replies
306
views
Should I use unpaired reads from trimmomatic
RNA-seq
QC
Trimmomatic
updated 4 days ago by
swbarnes2
14k • written 5 days ago by
dxj294
• 0
184 results • Page
1 of 4
Recent Votes
Comment: Help understanding how KEGG Ortholog `K00004 ` has 3 ECs associated with it (EC:
A: Deeptools plotHeatmap - Maintain Order of Input BED file
Comment: NGS forensics: how to know if data is fabricated
Comment: calculate nucleotide diversity from whole-genome-sequence data for individual ge
Comment: calculate nucleotide diversity from whole-genome-sequence data for individual ge
Answer: DNA methylation preprocessing
Comment: NGS forensics: how to know if data is fabricated
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Recent Replies
Comment: gvcf joint calling
by
zihanss
• 0
Thanks for your comment, I get it now.
Answer: ChIP-seq datasets: input samples omitted?
by
ATpoint
82k
In my hands (and from what I know based on many years here) inputs are almost exclusively used during peak calling to correct for loci-spec…
Comment: How to assign cell types after integration in scRNA
by
ATpoint
82k
No difference here. After all you typically want a label per cluster/group, so either subset to the control condition to do the assignment,…
Comment: Differential Expression using Isoseq-supplemented reference transcriptome
by
gaoanwei
• 0
I found the question and ASK the GPT4 turbo
Answer: Differential Expression using Isoseq-supplemented reference transcriptome
by
gaoanwei
• 0
Your approach seems reasonable and well-informed, though it's perhaps less customary in the field, which could be why you haven't found pap…
Answer: How many reads for WGS Sequencing?
by
Mensur Dlakic
★ 27k
It takes 29 seconds to assemble this genome (20 CPUs) with the following statistics: 135 contigs, total 2821177 bp, min 200 bp, max …
Comment: NGS forensics: how to know if data is fabricated
by
noodle
▴ 520
IMO (and unfortunately) there needs to be an effort to develop these algorithms.
Comment: gvcf joint calling
by
Jeremy Leipzig
22k
the samples that are `./.` have no coverage (or not enough to call a genotype) and the `0/0` are homozygous reference
Answer: How many reads for WGS Sequencing?
by
GenoMax
141k
Did you download the complete dataset available from ENA/NCBI SRA? This is an older dataset (from 2012) with a total of 1146212 reads and 1…
Comment: When to use .vcf or .gvcf files from GATK HaplotypeCaller?
by
zihanss
• 0
Hello, I want to know that why my gVCF files have "./." besides "0/0", "1/1"? Thanks
Comment: gvcf joint calling
by
zihanss
• 0
![enter image description here][1] [1]: /media/images/15eedc1a-b2c6-4966-be39-b5173dab And I confused with the file that has "./." and…
Comment: gvcf joint calling
by
zihanss
• 0
![enter image description here][1] [1]: /media/images/16fd502c-4e01-4f56-8562-0e0d4aac Okay, this is the merged gVCF file.
Comment: Help understanding how KEGG Ortholog `K00004 ` has 3 ECs associated with it (EC:
by
Mensur Dlakic
★ 27k
Enzymes under the umbrella of `1.1.1.-` work `with NAD(+) or NADP(+) as acceptor`. That only tells you about their cofactors, but not about…
Comment: NGS forensics: how to know if data is fabricated
by
dsull
★ 5.9k
I don't think people have undertaken the effort to create an anomaly detector for RNAseq -- people's efforts are dedicated towards developi…
Comment: NGS forensics: how to know if data is fabricated
by
dsull
★ 5.9k
I'd say post on pubpeer -- it's the best forum for this sort of discussion. As for what additional analysis I recommend: I'd say look at s…
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