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13,538 results • Page
4 of 271
Sort: Votes
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Votes
Replies
40
votes
35
replies
13k
views
10 follow
find positions of a short sequence in a genome
sequence
search
updated 8 months ago by
Mark
★ 1.5k • written 7.7 years ago by
igor
13k
40
votes
10
replies
42k
views
8 follow
Batch effects : ComBat or removebatcheffects (limma package) ?
limma
sva
Combat
batch-effect
updated 3 hours ago by
cwwong13
▴ 40 • written 6.7 years ago by
lessismore
★ 1.3k
39
votes
6
replies
19k
views
Tutorial:
Creating chromosome karyotype plot with R and ggplot2
ggplot2
R
chromosome
karyotype
updated 11 months ago by
Ram
43k • written 6.7 years ago by
steve
★ 3.5k
39
votes
15
replies
23k
views
9 follow
How Do I Access And Query Entire Genome Sequences With R
gene
r
updated 5 months ago by
Ram
43k • written 14.1 years ago by
John
▴ 790
39
votes
21
replies
13k
views
10 follow
Forum:
Orientation of PE reads a review of --fr --ff and --rf meanings
RNA-Seq
updated 11 months ago by
Ram
43k • written 5.5 years ago by
Juke34
8.5k
39
votes
11
replies
9.1k
views
11 follow
Where Can I Get The Secondary Structure Of A Protein?
protein
structure
sequence
updated 6 months ago by
Ram
43k • written 14.2 years ago by
Giovanni M Dall'Olio
28k
39
votes
18
replies
6.0k
views
10 follow
Any Open Notebook Science In Bioinformatics?
open-notebook
updated 5 months ago by
Ram
43k • written 14.0 years ago by
Heather Piwowar
▴ 380
39
votes
23
replies
34k
views
11 follow
bcftools: error while loading shared libraries: libcrypto.so.1.0.0: cannot open shared object file: No such file or directory
bcftools
updated 8 months ago by
s.w.vanderlaan
▴ 40 • written 2.8 years ago by
Michal Nevo
▴ 130
39
votes
16
replies
36k
views
16 follow
Tutorial:
installing samtools with conda - overcoming two common errors
samtools
conda
updated 4 months ago by
GenoMax
141k • written 3.7 years ago by
lavinia.gordon
▴ 160
39
votes
28
replies
11k
views
10 follow
How Much Does It Cost To Align A Flowcell In The Cloud?
cloud
12 weeks ago by
Jeremy Leipzig
22k
38
votes
21
replies
19k
views
8 follow
Tool:
pybam - 100% python BAM reader
python
htspython
bam
pysam
updated 10 months ago by
Ram
43k • written 8.0 years ago by
John
13k
38
votes
14
replies
9.1k
views
8 follow
Is There A Non-Perl Alternative To Accessing Ensembl'S Api?
ensembl
python
biopython
genome
updated 5 months ago by
Ram
43k • written 14.1 years ago by
Rvidal
▴ 270
38
votes
25
replies
9.1k
views
13 follow
FASTA file of fixed length
sequence
fasta
updated 7 months ago by
Ram
43k • written 8.1 years ago by
waqasnayab
▴ 250
38
votes
12
replies
18k
views
8 follow
BEDOPS gtf2bed conversion error with Ensembl GTF
gtf2bed
ensembl
bedops
gtf
updated 8 weeks ago by
Alex Reynolds
35k • written 7.7 years ago by
bioinformatics.cancer
▴ 260
38
votes
17
replies
3.2k
views
7 follow
Forum:
Why does bioinformatics use a special format like a BAM file instead of a database like SQLite?
sam
bam
samtools
updated 10 months ago by
Ram
43k • written 3.7 years ago by
kojix2
▴ 250
38
votes
7
replies
2.3k
views
News:
2 last seats available - 7th Berlin Summer School in NGS Data Analysis (September 4-8 in Berlin)
Transcriptomics
DNA-Seq
Variant-Calling
RNA-Seq
9 months ago by
David Langenberger
11k
38
votes
11
replies
4.1k
views
7 follow
Python Documentation Tools
python
updated 10 months ago by
Ram
43k • written 13.9 years ago by
Thaman
★ 3.3k
38
votes
19
replies
11k
views
Tool:
Excel add-in for DNA/RNA string manipulations
sequence
DNA
RNA
Excel
updated 10 months ago by
Ram
43k • written 8.1 years ago by
jgbaum
▴ 140
38
votes
26
replies
19k
views
16 follow
GO analysis: DAVID vs GREAT vs GOrilla
RNA-Seq
ChIP-Seq
GO
gene ontology
written 8.2 years ago by
biostart
▴ 370
38
votes
8
replies
1.6k
views
6 follow
News:
What happens when a bioinformatician has his own genome sequenced for $300?
DNAseq
DNA
WGS
updated 10 months ago by
Alex Reynolds
35k • written 10 months ago by
David Langenberger
11k
37
votes
20
replies
7.2k
views
14 follow
Online Bioinformatics Resources
online-resources
updated 5 months ago by
Ram
43k • written 14.0 years ago by
Eric Normandeau
11k
37
votes
9
replies
4.5k
views
8 follow
What are the advantages of using the T2T as a reference vs GRCh38 today?
reference
GRCh38
t2t
calling
variant
updated 11 months ago by
LauferVA
4.2k • written 12 months ago by
onter
▴ 170
37
votes
5
replies
6.1k
views
Tutorial:
[Beginner] Introduction to bioinformatics file types
fastq
fasta
bed
SAM
updated 7 months ago by
gallardodiazmiriam
▴ 20 • written 8.8 years ago by
Luke Crichton
▴ 430
37
votes
5
replies
13k
views
How do you generate TMM normalized counts using EdgeR?
R
RNA-seq
edgeR
updated 19 days ago by
inedraylig
▴ 60 • written 2.9 years ago by
Pratik
★ 1.0k
37
votes
16
replies
4.0k
views
9 follow
Forum:
Sanger sequencing is no longer the gold standard?
Sanger
NGS
updated 11 months ago by
Ram
43k • written 6.5 years ago by
lamteva.vera
▴ 220
36
votes
34
replies
2.8k
views
9 follow
Forum:
New to bioinformatics.....
gene
updated 11 months ago by
Ram
43k • written 6.1 years ago by
sms.00196
• 0
36
votes
39
replies
4.1k
views
11 follow
Forum:
Survey/Vote: If you could double the speed of any three commandline tools, which three would they be?
blast
RNA-Seq
alignment
next-gen-sequencing
updated 11 months ago by
Ram
43k • written 6.5 years ago by
dhbradshaw
▴ 130
36
votes
25
replies
32k
views
15 follow
Downsampling dataset with more than 60 million reads
next-gen
RNA-Seq
updated 4 months ago by
asalimih
▴ 60 • written 9.7 years ago by
mike
▴ 90
36
votes
17
replies
3.7k
views
9 follow
Which Software Development Technique Is Used In Your Lab?
subjective
updated 5 months ago by
Ram
43k • written 14.1 years ago by
Giovanni M Dall'Olio
28k
36
votes
13
replies
7.3k
views
8 follow
How Do You Log Details Of Data Processing/ Pipelines / In Silico Analyses Performed
data
galaxy
written 13.0 years ago by
Pi
▴ 520
36
votes
16
replies
14k
views
8 follow
How to identify 16s sequences from binning data(contigs)?
genome
Assembly
sequence
blast
updated 9 months ago by
Kermit
▴ 90 • written 8.6 years ago by
liuyifan2014
▴ 110
36
votes
11
replies
33k
views
6 follow
Sjdboverhang Option In Star
updated 9 months ago by
Kermit
▴ 90 • written 10.2 years ago by
Martombo
★ 3.1k
35
votes
17
replies
16k
views
12 follow
Tutorial:
Machine Learning For Cancer Classification - Part 3 - Predicting With A Random Forest Classifier
machine-learning
classification
updated 12 months ago by
GenoMax
141k • written 10.4 years ago by
Obi Griffith
20k
35
votes
10
replies
16k
views
11 follow
Which Chip Seq Peak Callers Do You Use?
chip-seq
peak-calling
updated 5 months ago by
Ram
43k • written 14.0 years ago by
Stew
★ 1.4k
35
votes
14
replies
44k
views
9 follow
How to filter vcf file on minimum genotype depth and quality for each sample
vcf
bcftools
updated 7 months ago by
ashotmarg2004
▴ 130 • written 7.8 years ago by
William
★ 5.3k
35
votes
15
replies
12k
views
10 follow
How to select an aligner?
RNA-Seq
next-gen
alignment
updated 4 months ago by
Erfan
• 0 • written 5.9 years ago by
marongiu.luigi
▴ 710
34
votes
23
replies
21k
views
12 follow
Is there a tool that sorts gtf files?
RNA-Seq
genome
updated 7 months ago by
alejandrogzi
▴ 120 • written 6.1 years ago by
JJ
▴ 680
34
votes
16
replies
20k
views
9 follow
Filtration Of Reads With Length Lower Than 30 From Bam
read
length
bam
updated 10 days ago by
Hagen
▴ 10 • written 10.2 years ago by
filipzembol
▴ 180
34
votes
5
replies
2.3k
views
Forum:
what we should reply when we are asked do you have any question?
career
interview
updated 11 months ago by
Ram
43k • written 6.9 years ago by
zizigolu
★ 4.3k
34
votes
22
replies
2.9k
views
11 follow
Forum:
Have we failed as bioinformatician in this time of COVID-19
Bioinformatician
COVID-19
updated 10 months ago by
Ram
43k • written 4.0 years ago by
heididunst
▴ 10
34
votes
22
replies
1.5k
views
10 follow
NGS forensics: how to know if data is fabricated
fastq
STAR
NGS
Illumina
5 hours ago by
noodle
▴ 570
34
votes
11
replies
45k
views
8 follow
Where Can I Download Some Bam Files?
bam
RNA-seq
updated 4 months ago by
Ram
43k • written 12.0 years ago by
snakesgun
▴ 160
34
votes
8
replies
2.7k
views
9 follow
Forum:
Bioinformatician, a jack of all trades, but in the eye of the CV-beholder a master of none? How can your CV reflect the diverse skills that you have …
cv
industry
career
updated 10 months ago by
Ram
43k • written 4.5 years ago by
'
▴ 300
34
votes
9
replies
11k
views
7 follow
Tool:
Bwa-Meth: Align And Tabulate Bs-Seq Reads
methylation
updated 10 months ago by
Ram
43k • written 10.2 years ago by
brentp
24k
34
votes
16
replies
20k
views
14 follow
Tutorial:
TCGA UUIDS to TCGA barcode (SampleID) in R
next-gen
GDC
R
TCGA
updated 5 weeks ago by
aUser
▴ 30 • written 7.6 years ago by
martinguerrerog89
▴ 310
34
votes
19
replies
47k
views
11 follow
Convert Refseq Id To Gene Name
refseq
gene
updated 9 months ago by
Ram
43k • written 10.9 years ago by
hicsuntdrac0nis
▴ 250
33
votes
23
replies
4.2k
views
8 follow
Forum:
Is there a file format better suited for the era of pangenomics than the .vcf? What are its attributes?
VCF
file-format
genomics
updated 10 months ago by
Jeremy Leipzig
22k • written 10 months ago by
LauferVA
4.2k
33
votes
9
replies
10k
views
10 follow
Which C++ Libraries Are Best For Dealing With Fastq Files?
next-gen-sequencing
fastq
c
updated 5 months ago by
Ram
43k • written 14.1 years ago by
Jeremy Leipzig
22k
33
votes
3
replies
15k
views
Tool:
Aligning Sequence Reads, Clone Sequences And Assembly Contigs With Bwa-Mem
bwa
ngs
updated 10 months ago by
Ram
43k • written 11.1 years ago by
lh3
33k
33
votes
10
replies
35k
views
8 follow
Convert between RefSeq and Ensembl Transcript?
refseq
ensembl
updated 9 months ago by
Ram
43k • written 9.8 years ago by
pwg46
▴ 540
13,538 results • Page
4 of 271
Recent Votes
Comment: High Malat-1 expression in single cell data
Answer: Extract protein sequence
Answer: Extract protein sequence
C: How do I ask a question on Biostars?
Answer: Source other conda environments in a nextflow pipeline when nextflow itself is i
Answer: Source other conda environments in a nextflow pipeline when nextflow itself is i
Comment: How to calculate identity percentage between proteins contained in a FASTA file?
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Comment: Perfom a Gene Ontology Analysis from GO terms txt file
by
sansan_96
▴ 80
Your table should look like this (GO terms): ``` geneid GOid Zm00001eb000010 GO:0003690;GO:0003727;GO:0019843;GO:0005739 Zm00001eb000020 G…
Answer: Perfom a Gene Ontology Analysis from GO terms txt file
by
sansan_96
▴ 80
I understand, here is an example with topGO where you need a differential expression table (DESeq2 for example) and a table of GO terms: `…
Comment: Perfom a Gene Ontology Analysis from GO terms file
by
Hamtaro
▴ 50
Thank u for your reply. The problem is that I used the genome and gene annotation from that webpage, not ensemble or ncbi, so I have to use…
Comment: Perfom a Gene Ontology Analysis from GO terms file
by
sansan_96
▴ 80
Hello, To perform functional enrichment analysis there are packages such as topGO or clusterProfiler that can work very well. Lately I've …
Answer: Post-imputation plot
by
LChart
3.9k
The anti-diagonal band does suggest some fraction of genotypes are strand-flipped - probably G/C or A/T variants.
Comment: Differential Accessibility
by
Ram
43k
Please do not paste screenshots of plain text content, it is counterproductive. You can copy paste the content directly here (using the cod…
Comment: Extract protein sequence
by
anna
▴ 20
Thank you! I need this for eukaryotic organism and known protein sequences, but I guess seqkit would work well!
Comment: Landmark gene selection in L1000.
by
GenoMax
141k
Paper referred to in question: https://pubmed.ncbi.nlm.nih.gov/29195078/ > so I want to understand how you reduced the 12063 genes to 978…
Comment: What should I consider as FASTA for dataset?
by
Nafi
• 0
Protein attribute prediction
Comment: Batch effects : ComBat or removebatcheffects (limma package) ?
by
cwwong13
▴ 40
@kevin May I know whether your comments and edits were mainly based on the fact that "Previously, [crazy] people had been using the origina…
Comment: Programmatically retrieving positions of protein active site residues
by
Wayne
★ 2.0k
Wow, I was shocked at this AI answer that looks to be a good start for using UniProt. Sharing it in case it is useful. (It will work in ses…
Comment: NGS forensics: how to know if data is fabricated
by
noodle
▴ 570
> Can you clarify what you mean by "100% of reads pass cutadapt, even > though 70% of reads contain adapters and get trimmed. The header o…
Comment: Software to separate reads from different individuals
by
GenoMax
141k
If you have independent information available (e.g. genotype data for both individuals) then you *may* be able to assign reads based on the…
Answer: Extract protein sequence
by
Matthias Zepper
4.5k
You didn't mention what kind of organism you are working with and what kind of protein you are looking for? If it happens to be a microbia…
Comment: How to use limma to find differentially expressed genes in response to a continu
by
pairedttest
▴ 10
Thank you Gordon for your expertise. One question that I have now that I have a list of DE genes is how to interpret logFC of this continuo…
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