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1,000 results • Page
4 of 20
Sort: Votes
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Views
Votes
Replies
33
votes
12
replies
1.1k
views
7 follow
Forum:
Nextflow and self-made pipelines opinion
nextflow
pipeline
data-analysis
10 weeks ago by
karlaarz
▴ 110
33
votes
23
replies
4.2k
views
8 follow
Forum:
Is there a file format better suited for the era of pangenomics than the .vcf? What are its attributes?
VCF
file-format
genomics
updated 11 months ago by
Jeremy Leipzig
22k • written 11 months ago by
LauferVA
4.2k
33
votes
5
replies
16k
views
9 follow
Forum:
How do I explain to my non-bioinformatician labmates how to interpret principal components?
RNA-Seq
communication
analysis
pca
updated 21 months ago by
Ram
43k • written 8.3 years ago by
Kristin Muench
▴ 620
32
votes
13
replies
11k
views
10 follow
Forum:
What Skills Do You Have To Have As A Phd Graduate In Bioinformatics?
genomics
phd
updated 14 months ago by
Ram
43k • written 11.8 years ago by
sentausa
▴ 650
32
votes
5
replies
5.6k
views
Forum:
ipython notebooks on bioinformatics and genomics
python
updated 13 months ago by
Ram
43k • written 8.9 years ago by
Pappu
★ 2.1k
32
votes
16
replies
12k
views
9 follow
Forum:
How to switch from bench research to computational biology?
career
updated 22 months ago by
Ram
43k • written 9.2 years ago by
yuggnurug
▴ 70
32
votes
14
replies
6.8k
views
9 follow
Forum:
Pat Schloss of Mothur uses this
uses-this
updated 14 months ago by
Ram
43k • written 9.5 years ago by
Istvan Albert
100k
32
votes
9
replies
5.9k
views
Forum:
Embrace The Non-Googlers Of Biostars
meta
biostars
updated 15 months ago by
Ram
43k • written 10.8 years ago by
KCC
★ 4.1k
31
votes
4
replies
5.6k
views
8 follow
Forum:
What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2016?
Assembly
sequencing
RNA-Seq
updated 13 months ago by
Ram
43k • written 7.3 years ago by
dago
★ 2.8k
31
votes
41
replies
2.7k
views
12 follow
Forum:
Question regarding journal publications
publications
journals
updated 11 months ago by
Ram
43k • written 4.2 years ago by
K.Gee
▴ 40
31
votes
16
replies
2.2k
views
8 follow
Forum:
What is in your opinion the current state-of-the-art sequencing platform for genomic assembly (Nov 2019)
next-gen-sequencing
assembly
genome
updated 11 months ago by
Ram
43k • written 4.5 years ago by
Antonio R. Franco
★ 5.1k
31
votes
13
replies
16k
views
8 follow
Forum:
Should I Pursue A Bioinformatics Msc?
career
updated 14 months ago by
Ram
43k • written 11.3 years ago by
biostepho
▴ 70
31
votes
7
replies
6.0k
views
9 follow
Forum:
Beginner in Bioinformatics / computational biology field
Bioinformatics
Computational-biology
updated 13 months ago by
Ram
43k • written 7.9 years ago by
tanvi.phaltane
▴ 70
31
votes
34
replies
7.5k
views
11 follow
Forum:
pros- and cons- : programming skills vs. GUI
programming
lecture
updated 13 months ago by
Ram
43k • written 8.4 years ago by
TriS
★ 4.7k
31
votes
8
replies
3.8k
views
6 follow
Forum:
Getting Things Done In Bioinformatics (More I Know, Less I Do?)
literature
analysis
updated 14 months ago by
Ram
43k • written 10.5 years ago by
Biomonika (Noolean)
3.2k
30
votes
13
replies
3.7k
views
11 follow
Forum:
what is your daily must see bioinformatics web site?
learn
technology
updated 11 months ago by
Ram
43k • written 9.7 years ago by
Medhat
9.7k
30
votes
5
replies
4.1k
views
Forum:
I'm applying for a non-PhD bioinformatics position in your lab. What do you look for?
sequencing
updated 2.5 years ago by
Ram
43k • written 9.8 years ago by
scical
▴ 100
30
votes
19
replies
10k
views
6 follow
Forum:
Programming language use distribution from recent programs / articles
programming-language
programs
updated 12 months ago by
Ram
43k • written 7.0 years ago by
Eric Normandeau
11k
30
votes
16
replies
2.3k
views
9 follow
Forum:
Which programming langauge shall I start as a beginner in Bioinformatics
programming-language
single-cell
updated 11 months ago by
Ram
43k • written 4.0 years ago by
nkg.bb
• 0
30
votes
27
replies
2.6k
views
11 follow
Forum:
How do you spend your time (productively) when your script is running?
time-management
updated 13 months ago by
Ram
43k • written 7.2 years ago by
venu
7.1k
30
votes
31
replies
3.8k
views
11 follow
Forum:
I need your help to find my future
internship
masters
career
updated 13 months ago by
Ram
43k • written 7.8 years ago by
Baptiste
▴ 90
29
votes
10
replies
3.2k
views
Forum:
How do you perform functional testing for your Bioinformatics tools?
development
testing
bug
reliability
updated 12 months ago by
Ram
43k • written 7.2 years ago by
shenwei356
8.5k
29
votes
18
replies
2.7k
views
12 follow
Forum:
what are the big questions in bioinformatics field nowadays
genome
updated 11 months ago by
Ram
43k • written 4.2 years ago by
t4192
▴ 20
29
votes
17
replies
2.3k
views
6 follow
Forum:
Can Biostars use question template like Github issue/PR?
meta
biostars
template
updated 13 months ago by
Ram
43k • written 7.2 years ago by
shenwei356
8.5k
29
votes
10
replies
9.3k
views
Forum:
Principles of Tidy Data (Hadley Wickham) and the VCF format
vcf
tidy-data
updated 2.2 years ago by
Ram
43k • written 9.4 years ago by
Giovanni M Dall'Olio
28k
29
votes
10
replies
6.5k
views
10 follow
Forum:
Interesting/useful/beautiful bio sequence viz tools
viz
python
visualisation
updated 14 months ago by
Ram
43k • written 6.1 years ago by
Joe
21k
29
votes
16
replies
3.2k
views
Forum:
Making bioinformatics help and tutorials open
ensembl
updated 14 months ago by
Ram
43k • written 9.7 years ago by
Emily
23k
29
votes
18
replies
3.3k
views
7 follow
Forum:
Should I quit PhD program?
education
career
research
phd
updated 11 months ago by
Ram
43k • written 6.0 years ago by
George
▴ 30
29
votes
21
replies
6.3k
views
Forum:
PhD vs Field application scientist in industry
career
updated 13 months ago by
Ram
43k • written 7.7 years ago by
Govi988
▴ 80
29
votes
4
replies
7.8k
views
Forum:
Update to tbb (2021.1.1, conda-forge) breaks bowtie2 (bioconda)
software-error
bowtie2
bioconda
conda
tbb
updated 14 months ago by
Ram
43k • written 3.2 years ago by
mlbendall
▴ 240
29
votes
4
replies
6.9k
views
Forum:
Biostar Vs. Seqanswers: What Is The Difference?
biostars
updated 15 months ago by
Ram
43k • written 11.0 years ago by
jobinv
★ 1.1k
29
votes
13
replies
3.7k
views
6 follow
Forum:
Map of bioinformatics or who is bioinformatician.
self-study
updated 13 months ago by
Ram
43k • written 7.5 years ago by
dshulgin
▴ 260
28
votes
7
replies
1.9k
views
Forum:
Do you have favorite icons for bioinformatics?
ubuntu
updated 14 months ago by
Ram
43k • written 3.2 years ago by
kojix2
▴ 250
28
votes
14
replies
6.9k
views
9 follow
Forum:
How Do You Use Cloud Computing For Bioinformatics In 2013?
cloud
updated 14 months ago by
Ram
43k • written 10.9 years ago by
Eric Normandeau
11k
28
votes
15
replies
4.5k
views
8 follow
Forum:
Anyone Use R For Their Bioinformatics Work?
r
updated 14 months ago by
Ram
43k • written 10.5 years ago by
arronslacey
▴ 320
28
votes
11
replies
3.2k
views
9 follow
Forum:
Getting started with NGS analysis, common issues?
software-error
next-gen
updated 2.0 years ago by
Ram
43k • written 9.2 years ago by
Keith
▴ 50
28
votes
31
replies
5.8k
views
8 follow
Forum:
Biostar Ads Are Now Live. Feedback And Comments Are Sought.
biostars
updated 15 months ago by
Ram
43k • written 10.9 years ago by
Istvan Albert
100k
28
votes
13
replies
1.5k
views
6 follow
Forum:
Suppose you have a sequencer that can read the genome end-to-end. What aspects of bioinformatics will become irrelevant?
future
updated 17 months ago by
Ram
43k • written 7.6 years ago by
Ayaan
▴ 40
28
votes
1
reply
6.9k
views
Forum:
Bedops: The Fastest, Most Scalable, And Easily-Parallelizable Genome Analysis Toolkit!
bedops
genome-analysis
updated 14 months ago by
Ram
43k • written 10.9 years ago by
Alex Reynolds
35k
28
votes
15
replies
2.3k
views
12 follow
Forum:
Should bioinformaticians learn the scientific names of plants and animals?
sequence
updated 11 months ago by
Ram
43k • written 5.8 years ago by
ivan.radonov
▴ 10
28
votes
14
replies
8.4k
views
8 follow
Forum:
Should I work first in a Bioinformatics company or get a Ph. D.?
Biochemistry
PhD
career
updated 13 months ago by
Ram
43k • written 8.0 years ago by
mvasquez16
▴ 20
28
votes
13
replies
2.7k
views
Forum:
Are there any workshops which are tailored to non-beginners/intermediate bioinformatics?
workshop
updated 19 months ago by
Ram
43k • written 8.8 years ago by
Daniel
★ 4.0k
28
votes
7
replies
5.3k
views
Forum:
Open Source projects to contribute to
contribute
open-source
updated 2.0 years ago by
Ram
43k • written 9.3 years ago by
shane
▴ 90
27
votes
33
replies
3.8k
views
7 follow
Forum:
Biostars question catagories
statistics
biostars
meta
updated 13 months ago by
Ram
43k • written 7.6 years ago by
John
13k
27
votes
17
replies
5.1k
views
Forum:
Data sharing via Bittorrent is coming to Biostar
biostars
bittorrent
updated 15 months ago by
Ram
43k • written 9.8 years ago by
Istvan Albert
100k
27
votes
11
replies
3.7k
views
10 follow
Forum:
Which programming language to integrate several open source software into one working pipeline (software)
MEGA
pipeline
updated 2.4 years ago by
Ram
43k • written 9.8 years ago by
benjamin.hebisch
▴ 70
27
votes
21
replies
9.3k
views
9 follow
Forum:
Better for my career: Degree in computer science or Phd in bioinformatics?
computer-science
phd
updated 13 months ago by
Ram
43k • written 7.7 years ago by
BioinfGuru
★ 1.7k
27
votes
19
replies
4.6k
views
10 follow
Forum:
Google genomics cloud platform ?
genome
cloud
updated 13 months ago by
Ram
43k • written 8.9 years ago by
GouthamAtla
12k
27
votes
0
replies
8.1k
views
Forum:
Heng Li of BWA and Samtools uses this
uses-this
updated 14 months ago by
Ram
43k • written 9.6 years ago by
Istvan Albert
100k
27
votes
8
replies
4.8k
views
6 follow
Forum:
PhD in Bioinformatics: to continue, or not to continue? That is the question--
career
updated 14 months ago by
Ram
43k • written 10.0 years ago by
MAPK
★ 2.1k
1,000 results • Page
4 of 20
Recent Votes
course on Landscape Genomics at the EPFL in Lausanne (June 17-21)
course on Landscape Genomics at the EPFL in Lausanne (June 17-21)
cellranger error message
Comment: Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA
Network plot from expression data in R using igraph
Comment: RNA seq analysis
RNA seq analysis
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Recent Replies
Comment: Truncated metadata file report from ENA Portal API
by
GenoMax
142k
Are you missing a `"` at the end of the URL. Doing wget -O test.out "https://www.ebi.ac.uk/ena/portal/api/filereport?accession=PRJNA43…
Comment: Different output for read length
by
marco.barr
▴ 100
very strange, I double checked the BAM file and it is the same one I used for your request. Are the commands I wrote above correct? How is …
Comment: Different output for read length
by
Pierre Lindenbaum
161k
the first read as a length of 1747, i wonder how you can just find '50' $ wget -qO - "https://gist.githubusercontent.com/Macdot3/4650…
Comment: Problem with Seurat package in PercentageFeatureSet function
by
bk11
★ 2.4k
Yes, `CreateAssayObject` function is used for only expression matrix.
Comment: Different output for read length
by
marco.barr
▴ 100
Here is my result, I hope you can see it. [https://gist.github.com/Macdot3/4650243a10540d8c06f728ffc16a136c][1] [1]: https://gist.gith…
Comment: Different output for read length
by
marco.barr
▴ 100
I just published my link on paste, why can't I see my public paste on the site?
Comment: cellranger error message
by
Max
• 0
I'm having a similar issue. The sample_id matches the filename prefix before _S1_L001, but I get the error: Log message: Requested sample(s…
Comment: Different output for read length
by
marco.barr
▴ 100
Thanks so much GenoMax, I'm sharing the link as you told me
Comment: Different output for read length
by
GenoMax
142k
Post the data at pastebin.com and add a link here or you could a GitHub gist. You were suspended by biostars SPAM bot since you pasted link…
Comment: Different output for read length
by
GenoMax
142k
Post the data at pastebin.com and add the link here. You were suspended by biostars SPAM bot since you pasted these links in quick successi…
Comment: RNA seq analysis
by
s.ghazala
• 0
Could you clarify on what exactly you are referring to with "SRA data table". If you are retrieving fastq files (raw data), then you could …
Comment: Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA
by
sovrappensiero
▴ 100
Thanks, Chris. I really appreciate your help. This has clarified a lot for me!
Answer: Snakemake fails to find conda in PBS
by
tim.booth
▴ 50
Snakemake does not have support for micromamba. There is an open issue: https://github.com/snakemake/snakemake/issues/2322
Comment: absolute path for symbolic links in Snakefile
by
tim.booth
▴ 50
Further to this answer - if you are using GNU coreutils (ie. any modern Linux), there is a "-r" flag to fix this problem. I typically use "…
Answer: Different output for read length
by
Pierre Lindenbaum
161k
I supect your reads are hard clipped. Show us the first lines of samtools view /home/sorted.bam | cut -f 2,6,10 | head -n 20
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