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117,470 results • Page
2 of 2350
Sort: Rank
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Votes
Replies
0
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2
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218
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METAL ERROR: The command you issued could not be processed ...
meta-analysis
METAL
GWAS
updated 2 days ago by
Ram
44k • written 3 days ago by
JJ
• 0
1
vote
1
reply
198
views
dbSNP download and merge to my data
SNP
GWAS
dbSNP
updated 3 days ago by
GenoMax
143k • written 3 days ago by
SeoG
• 0
4
votes
2
replies
295
views
NGS Data Analysis
NGS
Genome
sequencing
updated 3 days ago by
a.alnawfal.1992
▴ 290 • written 4 days ago by
Bindiya
▴ 10
0
votes
1
reply
267
views
Annotating Pathways in a network
Pathways
Cytoscape
Network
updated 3 days ago by
Scooter
▴ 280 • written 6 days ago by
Akash D
▴ 60
0
votes
2
replies
325
views
Enrichment Analysis of Pathways
Enrichment-Analysis
cytoscape
updated 3 days ago by
Scooter
▴ 280 • written 6 days ago by
Akash D
▴ 60
2
votes
5
replies
457
views
Salmon ~ Effective Length
Salmon
updated 3 days ago by
Rob
6.7k • written 19 days ago by
chrisk
• 0
2
votes
5
replies
1.1k
views
MAKER2 no FASTAs generated
MAKER
FASTA
fasta_merge
genome annotation
updated 3 days ago by
Kinoppy
• 0 • written 4.1 years ago by
jamie.pike
▴ 80
0
votes
1
reply
165
views
vg giraffe, accept admixed single and paired reads as input
vg
updated 3 days ago by
Jordan M Eizenga
▴ 480 • written 3 days ago by
Thomas
• 0
0
votes
0
replies
125
views
vg search for all specific kmer/motif (e.g., RE site) occurrences in pangenome graph
vg
3 days ago by
Thomas
• 0
2
votes
2
replies
263
views
Phasing human samples with Shapeit4
Phasing
Shapeit
Shapeit4
2 days ago by
Private
▴ 10
0
votes
4
replies
265
views
In silico PCR - multiple Primers and multiple sequences at the same time
help
pcr
pcrinsilico
updated 11 hours ago by
noodle
▴ 590 • written 3 days ago by
sulichkarolina
• 0
1
vote
1
reply
156
views
scaling for kallisto estimate counts
Differential-gene-expression
RNAlysis
Kallisto
3 days ago by
Meghan.T
• 0
2
votes
3
replies
427
views
Submitting to GEO: 10x Fixed RNA profiling (Single Cell Gene Expression Flex)
rna-seq
updated 3 days ago by
matthewsmith
▴ 10 • written 11 days ago by
psm
▴ 130
0
votes
0
replies
160
views
Tutorial:
DiffBind run parallel
CHIP-seq
ATAC-seq
DiffBind
updated 3 days ago by
ATpoint
83k • written 3 days ago by
Juan
• 0
2
votes
1
reply
167
views
Reformatting Fastq to Fasta for Metagenomic Analysis
metagenomics
fasta
fastq
updated 3 days ago by
Ram
44k • written 3 days ago by
echolley
• 0
1
vote
1
reply
173
views
MGI DNBelab C Series High-throughput Single-cell RNA Library Preparation Set V3.0, Seurat
rna-seq
seurat
single-cell
mgi
updated 3 days ago by
bk11
★ 2.7k • written 4 days ago by
yau
• 0
0
votes
7
replies
377
views
Looking for clarification of .vcf output from variant calling
variant
calling
vcf
freebayes
variants
updated 2 days ago by
Jeremy Leipzig
22k • written 4 days ago by
Jess
▴ 20
0
votes
0
replies
119
views
KEGG pathways analysis (ko and map IDs)
ko
kegg
pathway
map
updated 4 days ago by
GenoMax
143k • written 4 days ago by
manekineko
▴ 10
0
votes
3
replies
243
views
circlize R package to plot ecDNA
ecDNA
plot
circlize
updated 2 days ago by
cmdcolin
★ 3.8k • written 4 days ago by
a.loka92
• 0
0
votes
2
replies
186
views
Determining biological relevance of a list of motifs?
motifs
HOMER
3 hours ago by
Ronin
▴ 10
0
votes
4
replies
457
views
Is using the assembled transcript in one de novo assembler as reference/trusted contigs for the second assembler logical?
Trinity
RNA-seq
rnaSPAdes
updated 4 days ago by
GenoMax
143k • written 4 days ago by
RCMC
• 0
6
votes
6
replies
353
views
Best way to create a custom reference for CellRanger when using a transgenic mouse strain expressing a human protein.
cellranger
updated 3 days ago by
ATpoint
83k • written 4 days ago by
Alx
• 0
0
votes
0
replies
112
views
RMSD vs SDM (structural distance)
RMSD
4 days ago by
Coralie
• 0
1
vote
2
replies
205
views
cluster annotation in single-cell (-nuclei) RNA-Seq or multiome experiments
celldex
annotation
singler
scRNAseq
clustering
2 days ago by
gogeni5529
▴ 50
0
votes
2
replies
518
views
Speed of Cell-Cell interaction analysis on scRNA-seq data
Cellchat
scRNA-seq
Seurat
updated 4 days ago by
gglim
▴ 160 • written 12 months ago by
miyeok
• 0
0
votes
0
replies
113
views
Overrepresentation analysis for fractions
overrepresentation
4 days ago by
malonzm1
▴ 20
1
vote
1
reply
1.5k
views
Uniprot SPARQL: InterPro domain positions
sparql
updated 4 days ago by
me
▴ 760 • written 6.1 years ago by
fabian.grammes
▴ 10
1
vote
12
replies
838
views
6 follow
blast Segmentation fault
blastn
fasta
local-blast
updated 15 hours ago by
Ram
44k • written 3 months ago by
giulia.cosenza
▴ 10
0
votes
2
replies
190
views
Ubuntu 24.04 for Oxford Nanopore Mk1B
Ubuntu
installation
Software
3 days ago by
Calvin-Calvin
• 0
0
votes
5
replies
236
views
sam2tsv add in my path
sam2tsv
updated 3 days ago by
Pierre Lindenbaum
162k • written 4 days ago by
harry
▴ 30
3
votes
3
replies
231
views
FastQC report
quality
fastqc
filtration
updated 3 days ago by
a.alnawfal.1992
▴ 290 • written 4 days ago by
analyst
▴ 50
0
votes
6
replies
304
views
Stuck on how to run STARsolo on paired cDNA RNA-Seq FASTQ files
STARsolo
FASTQ
2 days ago by
Nicholas
• 0
0
votes
0
replies
92
views
PCa Biplot For RNA-Seq Study Samples With 1k Genome Reference Panel
ethnicity
snp
hapmap
4 days ago by
ssankar3
• 0
1
vote
1
reply
152
views
ROH and Manhattan plot
ROH
Manhattan
plink
updated 4 days ago by
bk11
★ 2.7k • written 4 days ago by
Emy
▴ 50
0
votes
7
replies
8.2k
views
7 follow
Using bcftools to change ploidy level in vcf files
snp
bcftools
updated 4 days ago by
michael
▴ 10 • written 7.1 years ago by
flolil27
• 0
0
votes
0
replies
99
views
What is the reason for the requirement of at least 2 experiments for LFQ in MaxQuant?
MaxQuant
Spectrometry
Mass
Proteomics
LFQ
4 days ago by
Riq
▴ 50
2
votes
1
reply
152
views
Regarding WGCNA
WGCNA
updated 4 days ago by
Ram
44k • written 4 days ago by
deepak
• 0
0
votes
6
replies
344
views
Why am I getting such a big difference in alignment rates in kallisto?
kallisto
ensembl
3 days ago by
bioinfo
▴ 150
0
votes
1
reply
168
views
Reading files from different types as H5AD
python
scanpy
anndata
single-cell
updated 4 days ago by
bk11
★ 2.7k • written 4 days ago by
JACKY
▴ 140
1
vote
5
replies
2.0k
views
Error in converting bed file to interval using Picard
WES
exome
BedToIntervalList
picard
agilent
updated 4 days ago by
Ram
44k • written 4.6 years ago by
Assa Yeroslaviz
★ 1.9k
0
votes
0
replies
197
views
maftools oncoplot top bar barplots - any way to customize this plot further with color aesthetics or cutoff lines?
maf
maftools
oncoplot
exome
updated 4 days ago by
Ram
44k • written 4 days ago by
acererak
▴ 10
0
votes
4
replies
244
views
Help with CNA analysis using control-FREEC
CNA
control-FREEC
4 days ago by
sainavyav22
• 0
1
vote
0
replies
199
views
Job:
Seeking remote intership/job
remote
europe
updated 5 days ago by
Ram
44k • written 5 days ago by
giulia.cosenza
▴ 10
1
vote
2
replies
202
views
Access to Giraffe sequence data
nature
giraffe
data-access
updated 5 days ago by
Ram
44k • written 5 days ago by
Hansen_869
▴ 80
0
votes
1
reply
155
views
Best kit for PacBio HiFi Sequencing preperation
PacBio-sequencing
PacBio-HiFI
HMW-gDNA
updated 4 days ago by
Ram
44k • written 5 days ago by
Human
• 0
1
vote
1
reply
165
views
Diffbind fold values and parameter Fold in DBA.report function
DESEQ2
ATAC-seq
DBA.report
Diffbind
5 days ago by
Manko47
▴ 10
0
votes
1
reply
187
views
Conservation scores at each location of few motifs in MSA
conservation-scores
FASTA
MSA
updated 5 days ago by
zx8754
11k • written 5 days ago by
swathi.graduate1998
• 0
0
votes
1
reply
153
views
How lambda parameter works in BLAST?
lambda
BLAST
updated 4 days ago by
biofalconch
★ 1.1k • written 5 days ago by
jqg44605
• 0
6
votes
3
replies
301
views
GSEA with fgsea package and using stat from DESeq2 output - what to do with NA padj values?
RNA-seq
DESeq2
GSEA
4 days ago by
Fossil
▴ 20
0
votes
1
reply
238
views
Editing seurat object metatdata
Seurat
metadata
scRNA-seq
updated 5 days ago by
Nat.Nataren
▴ 40 • written 7 days ago by
Happiness
• 0
117,470 results • Page
2 of 2350
Recent Votes
Comment: Making heatmaps of ATACseq annotation data in R?
Answer: Failed to download data from EBI with ascp
Failed to download data from EBI BioStudies with ascp/Aspera
Comment: in R, how to get the gene symbols of GPL
C: Is my WGCNA soft threshold (power) too low?
edgeR batch effect model matrix sanity check
Answer: Alignment tool for very short reads (25bp)
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Recent Replies
Comment: Is T2T genome a "alternate assembly"
by
GenoMax
143k
You are downloading the correct file if you are interested in the T2T genome. My guess is that the assembly is labeled "alternate" likely…
Comment: postdoc/master's position AI ML
by
Ram
44k
Please invest some effort into formatting your post, don't just copy-paste. A lot can be lost and mistranslated when you copy from a web-pa…
Comment: Determining biological relevance of a list of motifs?
by
Ronin
▴ 10
Hi, sorry for the late response, I was ill. The setup is looking at a particular tissue (eye samples) in a non-model species, and I used th…
Comment: Failed to download data from EBI BioStudies with ascp/Aspera
by
ruiyan_hou
• 0
Thank you very much for your quick reply! It is very useful.
Comment: how linearize nucleotide lines in axt file
by
rfran010
▴ 960
I think that's a tough one. Will the two sequences under each header always be equal lengths? If so, I'm thinking a custom script that can …
Comment: Failed to download data from EBI with ascp
by
Michael
54k
I have tested and concluded that **there is a problem with Aspera download from** **BioStudies** (ENA and BioStudies are technically differ…
Comment: Slot to pull data from for DoHeatmap
by
Ming Tommy Tang
★ 4.0k
use the data slot, and as shown in the code, you then scale it by yourself.
Answer: Example human WGS fastq data
by
Ming Tommy Tang
★ 4.0k
Take a look at Genome in a Bottle https://www.nist.gov/programs-projects/genome-bottle
Answer: Download SRR by parallele and fastq dump with a wired problem
by
Ming Tommy Tang
★ 4.0k
I would suggest you to use fastq-dl https://github.com/rpetit3/fastq-dl to download at project level
Comment: Alignment tool for very short reads (25bp)
by
Ming Tommy Tang
★ 4.0k
use bowtie1 for short reads https://sourceforge.net/projects/bowtie-bio/files/bowtie/1.3.1/
Comment: In silico PCR - multiple Primers and multiple sequences at the same time
by
noodle
▴ 590
I understand you have a steep learning curve...you should learn how to use blast from a command line.
Comment: Failed to download data from EBI with ascp
by
ruiyan_hou
• 0
Hi Michael, I still cannot download. Actually I want to download E-MTAB-6798 from ENA. https://www.ebi.ac.uk/biostudies/arrayexpress/…
Comment: Alignment tool for very short reads (25bp)
by
arriyaz.nstu
▴ 50
Thank you very much for your reply.
Comment: Alignment tool for very short reads (25bp)
by
arriyaz.nstu
▴ 50
Thank you too for a very nice and clear suggestion. Sure, I will check the `bowtie2` options.
Comment: Help aligning Nanopore Long Read RNA seqs
by
rfran010
▴ 960
Thanks! I think you might be misinterpreting your coverage output. I believe that just indicates the chromosome and how many reads map to i…
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