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updated 3.2 years ago by
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written 3.2 years ago by
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3.2 years ago by
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updated 3.2 years ago by
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written 3.2 years ago by
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I tried using the main site but it still didn't work. Thanks anyway, I'll try other methods.
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If you want an overall taxonomic profile, this tool will do it from reads directly:
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I have used BURST (https://github.com/knights-lab/BURST) to do this before. You can take fastA sequences for your target genes and build ou…
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Why not just say "technical replicates are necessary"?
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Well, we already have mags, but my boss want to check the raw reads, that is why I am trying to find a way.
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Then you need to decide if you want to assemble your reads into contigs/MAG's followed by using the tools discussed. Or do the mapping usin…
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Thank you. It has not been my understanding or experience that calling variants in tumor-normal pairs is straightforward, especially somati…
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I don't think any kind of web-based tool will work; the read files are very large (at least 10 GB). Usually, web versions have restrictions…
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Kallisto is a pseudoaligner, so, as @atpoint suggested, it could be DNA contamination. You can confirm this using an aligner and a referenc…
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