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Limit : all time
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1,000 results • Page
4 of 20
Sort: Votes
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Views
Votes
Replies
33
votes
26
replies
3.6k
views
7 follow
Forum:
Poll: How many people have PyPy?
pypy
python
updated 13 months ago by
Ram
44k • written 8.2 years ago by
John
13k
33
votes
23
replies
4.3k
views
8 follow
Forum:
Is there a file format better suited for the era of pangenomics than the .vcf? What are its attributes?
VCF
file-format
genomics
updated 11 months ago by
Jeremy Leipzig
22k • written 11 months ago by
LauferVA
4.2k
33
votes
12
replies
1.2k
views
7 follow
Forum:
Nextflow and self-made pipelines opinion
nextflow
pipeline
data-analysis
11 weeks ago by
karlaarz
▴ 110
32
votes
16
replies
12k
views
9 follow
Forum:
How to switch from bench research to computational biology?
career
updated 23 months ago by
Ram
44k • written 9.2 years ago by
yuggnurug
▴ 70
32
votes
9
replies
5.9k
views
Forum:
Embrace The Non-Googlers Of Biostars
meta
biostars
updated 15 months ago by
Ram
44k • written 10.9 years ago by
KCC
★ 4.1k
32
votes
13
replies
11k
views
10 follow
Forum:
What Skills Do You Have To Have As A Phd Graduate In Bioinformatics?
genomics
phd
updated 15 months ago by
Ram
44k • written 11.8 years ago by
sentausa
▴ 650
32
votes
14
replies
6.8k
views
9 follow
Forum:
Pat Schloss of Mothur uses this
uses-this
updated 14 months ago by
Ram
44k • written 9.6 years ago by
Istvan Albert
100k
32
votes
5
replies
5.6k
views
Forum:
ipython notebooks on bioinformatics and genomics
python
updated 14 months ago by
Ram
44k • written 8.9 years ago by
Pappu
★ 2.1k
31
votes
16
replies
2.2k
views
8 follow
Forum:
What is in your opinion the current state-of-the-art sequencing platform for genomic assembly (Nov 2019)
next-gen-sequencing
assembly
genome
updated 11 months ago by
Ram
44k • written 4.6 years ago by
Antonio R. Franco
★ 5.1k
31
votes
4
replies
5.6k
views
8 follow
Forum:
What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2016?
Assembly
sequencing
RNA-Seq
updated 13 months ago by
Ram
44k • written 7.4 years ago by
dago
★ 2.8k
31
votes
8
replies
3.8k
views
6 follow
Forum:
Getting Things Done In Bioinformatics (More I Know, Less I Do?)
literature
analysis
updated 14 months ago by
Ram
44k • written 10.6 years ago by
Biomonika (Noolean)
3.2k
31
votes
34
replies
7.5k
views
11 follow
Forum:
pros- and cons- : programming skills vs. GUI
programming
lecture
updated 13 months ago by
Ram
44k • written 8.5 years ago by
TriS
★ 4.7k
31
votes
41
replies
2.8k
views
12 follow
Forum:
Question regarding journal publications
publications
journals
updated 11 months ago by
Ram
44k • written 4.2 years ago by
K.Gee
▴ 40
31
votes
7
replies
6.0k
views
9 follow
Forum:
Beginner in Bioinformatics / computational biology field
Bioinformatics
Computational-biology
updated 13 months ago by
Ram
44k • written 7.9 years ago by
tanvi.phaltane
▴ 70
31
votes
13
replies
16k
views
8 follow
Forum:
Should I Pursue A Bioinformatics Msc?
career
updated 15 months ago by
Ram
44k • written 11.3 years ago by
biostepho
▴ 70
30
votes
27
replies
2.6k
views
11 follow
Forum:
How do you spend your time (productively) when your script is running?
time-management
updated 13 months ago by
Ram
44k • written 7.3 years ago by
venu
7.1k
30
votes
5
replies
4.1k
views
Forum:
I'm applying for a non-PhD bioinformatics position in your lab. What do you look for?
sequencing
updated 2.5 years ago by
Ram
44k • written 9.9 years ago by
scical
▴ 100
30
votes
13
replies
3.8k
views
11 follow
Forum:
what is your daily must see bioinformatics web site?
learn
technology
updated 11 months ago by
Ram
44k • written 9.7 years ago by
Medhat
9.7k
30
votes
19
replies
10k
views
6 follow
Forum:
Programming language use distribution from recent programs / articles
programming-language
programs
updated 12 months ago by
Ram
44k • written 7.1 years ago by
Eric Normandeau
11k
30
votes
31
replies
3.8k
views
11 follow
Forum:
I need your help to find my future
internship
masters
career
updated 13 months ago by
Ram
44k • written 7.8 years ago by
Baptiste
▴ 90
30
votes
16
replies
2.3k
views
9 follow
Forum:
Which programming langauge shall I start as a beginner in Bioinformatics
programming-language
single-cell
updated 11 months ago by
Ram
44k • written 4.1 years ago by
nkg.bb
• 0
29
votes
10
replies
3.2k
views
Forum:
How do you perform functional testing for your Bioinformatics tools?
development
testing
bug
reliability
updated 12 months ago by
Ram
44k • written 7.2 years ago by
shenwei356
8.5k
29
votes
10
replies
6.5k
views
10 follow
Forum:
Interesting/useful/beautiful bio sequence viz tools
viz
python
visualisation
updated 14 months ago by
Ram
44k • written 6.2 years ago by
Joe
21k
29
votes
13
replies
3.7k
views
6 follow
Forum:
Map of bioinformatics or who is bioinformatician.
self-study
updated 13 months ago by
Ram
44k • written 7.5 years ago by
dshulgin
▴ 260
29
votes
18
replies
2.8k
views
12 follow
Forum:
what are the big questions in bioinformatics field nowadays
genome
updated 11 months ago by
Ram
44k • written 4.2 years ago by
t4192
▴ 20
29
votes
18
replies
3.3k
views
7 follow
Forum:
Should I quit PhD program?
education
career
research
phd
updated 12 months ago by
Ram
44k • written 6.0 years ago by
George
▴ 30
29
votes
4
replies
6.9k
views
Forum:
Biostar Vs. Seqanswers: What Is The Difference?
biostars
updated 15 months ago by
Ram
44k • written 11.1 years ago by
jobinv
★ 1.1k
29
votes
4
replies
7.9k
views
Forum:
Update to tbb (2021.1.1, conda-forge) breaks bowtie2 (bioconda)
software-error
bowtie2
bioconda
conda
tbb
updated 14 months ago by
Ram
44k • written 3.2 years ago by
mlbendall
▴ 240
29
votes
16
replies
3.2k
views
Forum:
Making bioinformatics help and tutorials open
ensembl
updated 14 months ago by
Ram
44k • written 9.8 years ago by
Emily
23k
29
votes
17
replies
2.3k
views
6 follow
Forum:
Can Biostars use question template like Github issue/PR?
meta
biostars
template
updated 13 months ago by
Ram
44k • written 7.2 years ago by
shenwei356
8.5k
29
votes
21
replies
6.3k
views
Forum:
PhD vs Field application scientist in industry
career
updated 13 months ago by
Ram
44k • written 7.8 years ago by
Govi988
▴ 80
29
votes
10
replies
9.3k
views
Forum:
Principles of Tidy Data (Hadley Wickham) and the VCF format
vcf
tidy-data
updated 2.2 years ago by
Ram
44k • written 9.5 years ago by
Giovanni M Dall'Olio
28k
28
votes
14
replies
8.4k
views
8 follow
Forum:
Should I work first in a Bioinformatics company or get a Ph. D.?
Biochemistry
PhD
career
updated 13 months ago by
Ram
44k • written 8.1 years ago by
mvasquez16
▴ 20
28
votes
7
replies
5.3k
views
Forum:
Open Source projects to contribute to
contribute
open-source
updated 2.1 years ago by
Ram
44k • written 9.3 years ago by
shane
▴ 90
28
votes
13
replies
2.7k
views
Forum:
Are there any workshops which are tailored to non-beginners/intermediate bioinformatics?
workshop
updated 19 months ago by
Ram
44k • written 8.8 years ago by
Daniel
★ 4.0k
28
votes
15
replies
4.5k
views
8 follow
Forum:
Anyone Use R For Their Bioinformatics Work?
r
updated 14 months ago by
Ram
44k • written 10.6 years ago by
arronslacey
▴ 320
28
votes
15
replies
2.3k
views
12 follow
Forum:
Should bioinformaticians learn the scientific names of plants and animals?
sequence
updated 12 months ago by
Ram
44k • written 5.9 years ago by
ivan.radonov
▴ 10
28
votes
14
replies
6.9k
views
9 follow
Forum:
How Do You Use Cloud Computing For Bioinformatics In 2013?
cloud
updated 14 months ago by
Ram
44k • written 10.9 years ago by
Eric Normandeau
11k
28
votes
13
replies
1.5k
views
6 follow
Forum:
Suppose you have a sequencer that can read the genome end-to-end. What aspects of bioinformatics will become irrelevant?
future
updated 17 months ago by
Ram
44k • written 7.6 years ago by
Ayaan
▴ 40
28
votes
1
reply
6.9k
views
Forum:
Bedops: The Fastest, Most Scalable, And Easily-Parallelizable Genome Analysis Toolkit!
bedops
genome-analysis
updated 15 months ago by
Ram
44k • written 11.0 years ago by
Alex Reynolds
35k
28
votes
11
replies
3.2k
views
9 follow
Forum:
Getting started with NGS analysis, common issues?
software-error
next-gen
updated 2.1 years ago by
Ram
44k • written 9.3 years ago by
Keith
▴ 50
28
votes
31
replies
5.8k
views
8 follow
Forum:
Biostar Ads Are Now Live. Feedback And Comments Are Sought.
biostars
updated 15 months ago by
Ram
44k • written 11.0 years ago by
Istvan Albert
100k
28
votes
7
replies
2.0k
views
Forum:
Do you have favorite icons for bioinformatics?
ubuntu
updated 14 months ago by
Ram
44k • written 3.2 years ago by
kojix2
▴ 250
27
votes
0
replies
8.1k
views
Forum:
Heng Li of BWA and Samtools uses this
uses-this
updated 14 months ago by
Ram
44k • written 9.6 years ago by
Istvan Albert
100k
27
votes
8
replies
4.8k
views
6 follow
Forum:
PhD in Bioinformatics: to continue, or not to continue? That is the question--
career
updated 14 months ago by
Ram
44k • written 10.0 years ago by
MAPK
★ 2.1k
27
votes
33
replies
3.8k
views
7 follow
Forum:
Biostars question catagories
statistics
biostars
meta
updated 13 months ago by
Ram
44k • written 7.6 years ago by
John
13k
27
votes
22
replies
5.8k
views
8 follow
Forum:
Recommendations of Linux distros for beginners in bioinformatics
training
linux
updated 12 months ago by
Ram
44k • written 6.5 years ago by
ropolocan
▴ 810
27
votes
11
replies
3.8k
views
10 follow
Forum:
Which programming language to integrate several open source software into one working pipeline (software)
MEGA
pipeline
updated 2.4 years ago by
Ram
44k • written 9.8 years ago by
benjamin.hebisch
▴ 70
27
votes
17
replies
5.2k
views
Forum:
Data sharing via Bittorrent is coming to Biostar
biostars
bittorrent
updated 15 months ago by
Ram
44k • written 9.9 years ago by
Istvan Albert
100k
27
votes
19
replies
4.6k
views
10 follow
Forum:
Google genomics cloud platform ?
genome
cloud
updated 14 months ago by
Ram
44k • written 8.9 years ago by
GouthamAtla
12k
1,000 results • Page
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Recent Votes
Answer: Question about samtools view flags (paired reads vs. properly paired reads)
Answer: Functional enrichment analysis for unique gene IDs
Comment: Super ehancers
Comment: Why does assigning genes with biomart give me different values than using a tran
Comment: Multiplexing for pooled CRISPR screen sequencing
Comment: How can we convert a vcf to fasta, so that I can blast some genes against that w
Answer: Differential Expression with limma: Contrast and Design Matrix, combinatorial ap
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Recent Replies
Comment: Genotyping sites with N in reference genome
by
shpak.max
▴ 50
As a follow-up, is it possible to get a variant call if rather than N, I have one of the ambiguity codes (e.g. R, Y, B etc) in the referenc…
Answer: Allele count of 2 for homoplasmic MT variants in VCF
by
LChart
3.9k
The GATK (and afaik every caller that I know) treats every contig of the reference as having the same ploidy (so all haploid, all diploid, …
Comment: Where are the illumina adapters on Trimmomatic take from?
by
GenoMax
142k
Use the sequence Illumina recommends for the specific kit.
Comment: Question about samtools view flags (paired reads vs. properly paired reads)
by
Pierre Lindenbaum
161k
> Thanks. What determines what constitutes a good distance? I think tools like BWA use the median distance for each chunk of processed d…
Comment: Where are the illumina adapters on Trimmomatic take from?
by
bioinfo
▴ 150
Thank you for the explanation. Is it more appropriate then to use the sequence from the illumina documentation or the one from the github?
Comment: Question about samtools view flags (paired reads vs. properly paired reads)
by
mrk
• 0
Thanks. What determines what constitutes a good distance? I have some targeted panel data and looking at flagstat, almost all reads are pai…
Answer: Where are the illumina adapters on Trimmomatic take from?
by
GenoMax
142k
Illumina indexed adapters share a core sequence at the beginning which is what is indicated in your link. Trimming programs will remove seq…
Comment: Allele count of 2 for homoplasmic MT variants in VCF
by
Ram
44k
Please stop using `bioinformatics` as a tag unless your post is about the field of bioinformatics itself.
Comment: Multiplexing for pooled CRISPR screen sequencing
by
GenoMax
142k
> If we switch to pair-end sequencing with dual-index barcode You can use dual-indexing with single end reads. You don't need to do paired…
Comment: Super ehancers
by
jared.andrews07
★ 17k
That is not valid GFF format, so that is probably going to be problematic. See the [GFF spec](https://github.com/The-Sequence-Ontology/Spec…
Comment: Error in CIBERSORTx ($ operator is invalid for atomic vectors)
by
finch
• 0
Hey! I had this issue and I think I can help- could you take a screenshot of the .txt file opened in the notepad?
Answer: Is it possible to run CibersortX with a very small number of samples?
by
finch
• 0
Hi! I had a very similar issue and it's likely not at all related to your number of samples but to the format of your mixture file! Ciberso…
Comment: How can we convert a vcf to fasta, so that I can blast some genes against that w
by
Ram
44k
You cannot *convert* a VCF to a FASTA as they have vastly different information content. Please explain your process - what exactly do you …
Comment: Comparing Analysis Strategies for scRNA-seq Data: Separate vs. Merged Analysis o
by
Shukai
• 0
Thank you Ram for the comments! Noted for future posts.
Comment: Where to find old version of GATK best practice
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Zhenyu Zhang
★ 1.2k
I am looking for diagrams, and step-by-step instructions of how to build my pipelines. I think the github is only helpful if you are a wdl…
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