updated 5.9 years ago by
Ram
43k
•
written 5.9 years ago by
abhilashreddy495
▴
10
9
votes
10
replies
3.5k
views
9
votes
4
replies
1.7k
views
9
votes
11
replies
6.6k
views
9
votes
6
replies
1.9k
views
updated 2.8 years ago by
wang-yanfang
•
0
•
written 4.6 years ago by
chichaochen
▴
30
9
votes
7
replies
3.6k
views
7.6 years ago by
statfa
▴
760
9
votes
13
replies
2.4k
views
single tumor vs multiple normal sample differential gene expression (RNA-Seq ) analysis using DESeq2
updated 3.0 years ago by
Ram
43k
•
written 3.0 years ago by
sumitguptabt
▴
30
9
votes
13
replies
3.4k
views
9
votes
5
replies
1.2k
views
9
votes
13
replies
1.6k
views
4.5 years ago by
imgapgenomika
▴
10
9
votes
16
replies
1.5k
views
updated 3.7 years ago by
lieven.sterck
15k
•
written 3.7 years ago by
schlogl
▴
160
9
votes
7
replies
5.4k
views
9
votes
10
replies
2.8k
views
3.3 years ago by
kwanghoon
▴
20
9
votes
2
replies
3.1k
views
8.1 years ago by
Shicheng Guo
★
9.4k
9
votes
5
replies
3.9k
views
9
votes
16
replies
1.9k
views
5.5 years ago by
bioinfo456
▴
150
9
votes
19
replies
2.4k
views
9
votes
9
replies
1.5k
views
4.9 years ago by
L. A. Liggett
▴
120
9
votes
5
replies
730
views
9
votes
13
replies
15k
views
7.2 years ago by
Patrick Brennan
▴
50
9
votes
20
replies
4.3k
views
updated 13 months ago by
Ram
43k
•
written 5.7 years ago by
marongiu.luigi
▴
710
9
votes
10
replies
1.9k
views
9
votes
8
replies
1.7k
views
6.9 years ago by
Jeffin Rockey
★
1.3k
9
votes
10
replies
2.2k
views
9
votes
2
replies
5.3k
views
updated 11.9 years ago by
Michael Kuhn
5.0k
•
written 11.9 years ago by
angeles.sepulvedap
•
0
9
votes
5
replies
3.8k
views
4.9 years ago by
cameron.holman
▴
20
8
votes
5
replies
1.7k
views
8
votes
6
replies
2.6k
views
8
votes
5
replies
12k
views
8
votes
12
replies
4.3k
views
8
votes
5
replies
831
views
8
votes
20
replies
2.7k
views
8
votes
8
replies
1.5k
views
5.3 years ago by
szabo.marton
▴
10
8
votes
14
replies
2.5k
views
6 follow
2.8 years ago by
johnsonqinz
•
0
8
votes
4
replies
2.3k
views
7.9 years ago by
Pierre Lindenbaum
161k
8
votes
11
replies
2.0k
views
8
votes
6
replies
2.3k
views
8
votes
3
replies
5.3k
views
updated 21 months ago by
Ram
43k
•
written 8.4 years ago by
dharshank.1096
▴
30
8
votes
14
replies
2.4k
views
6 follow
8
votes
13
replies
2.4k
views
8
votes
1
reply
8.6k
views
5.0 years ago by
CuriousGuy
▴
90
8
votes
4
replies
2.2k
views
12.8 years ago by
2184687-1231-83-
★
5.1k
8
votes
5
replies
3.6k
views
8
votes
7
replies
1.5k
views
8
votes
10
replies
2.0k
views
8
votes
9
replies
1.5k
views
6 follow
2.4 years ago by
zhangmeng21
▴
10
8
votes
30
replies
5.2k
views
updated 4.1 years ago by
wm
▴
560
•
written 4.1 years ago by
Researcher
▴
20
8
votes
9
replies
1.5k
views
2.9 years ago by
Alex
▴
20
8
votes
4
replies
864
views
8
votes
7
replies
3.9k
views
6 follow
5.6 years ago by
Batu
▴
250
8
votes
5
replies
14k
views
updated 5.7 years ago by
Ram
43k
•
written 8.2 years ago by
Peng Huang
▴
50
Recent Votes
Recent Locations •
All
France, 4 minutes ago
Belgium, 6 minutes ago
UK, 6 minutes ago
Romania, 7 minutes ago
France - Institut du Thorax, 7 minutes ago
Prague, 8 minutes ago
United Kingdom, 12 minutes ago
Recent Awards •
All
Popular Question to
ManuelDB
▴
80
Popular Question to
rustykb
▴
20
Popular Question to
sativus
▴
20
Popular Question to
kuttibiotech2009
▴
30
Popular Question to
analyst
▴
30
Recent Replies
Comment: RNA-seq data for deep learning classification
by
Yuju
•
0
Thank you very much for sharing your advice. Yes, it definitely makes sense that with the use of deep learning, models would learn normalis…
Comment: Generating mpileup file using samtools
by
Ruqaiya
•
0
I didn't use the same tool as in the paper...
Comment: read length in structural variant calling
by
eebloom
▴
80
Yes good idea. I guess the quality of the SV calls and as a proxy for quality the length and distribution of variants called might be infor…
Comment: Generating mpileup file using samtools
by
Ruqaiya
•
0
I just realised I didn't align my reads with the tools they used and used bowtie2 instead. I can't download the older version that is menti…
Comment: Downsampling long-read BAM files
by
eebloom
▴
80
This is not what I needed for this particular use case, as capping the coverage would lose the information on regions of copy number amplif…
Comment: Do I need to go back and filter my long-reads?
by
eebloom
▴
80
Apologies, I deleted the question as I wasn't sure it would be helpful to others and it didn't seem to have a clear answer, not to snub the…
Comment: Do I need to go back and filter my long-reads?
by
eebloom
▴
80
Thanks, I think it would be a good idea to track the results downstream to look for batch effects. I think I will filter the reads ultimate…
Comment: Generating mpileup file using samtools
by
ATpoint
82k
Seconding this. Apply current best practices (which is bcftools mpileup followed by something I forgot, see bcftools manual for variant cal…
Answer: Analysis of intronic reads included scRNA-seq data
by
ATpoint
82k
By default in CellRanger (lets assume you have 10x data processed with it) intronic reads are included. What you get in your matrix.mtx fil…
Comment: How does gene length effect the number of reads mapped
by
i.sudbery
19k
The number of reads for a gene is almost exactly linearly proportional to the length of the gene. In paired-end sequencing, we generally co…
Comment: What analysis suitable to identify similarly expressed genes between two samples
by
ATpoint
82k
Can you post your setup, so how many groups and their replication number? I can tell you by experience that you need even more replication …
Answer: DSEQ2 analysis
by
ATpoint
82k
The tool is called D**E**Seq2. Anyway, if your factor is `factor(conditions, levels = c("control", "mutant"))` then the first level is the …
Comment: Odd alignment question/finding
by
barslmn
★
2.1k
Maybe it is caused by fastp. https://github.com/OpenGene/fastp/issues/506
Comment: Generating mpileup file using samtools
by
Joe
21k
It's been a long time since I did this sort of thing, but I have a vague recollection that the mpileup process was made simpler and/or roll…
Traffic: 2220 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6