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411 results • Page
4 of 9
Sort: replies
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Votes
Replies
3
votes
3
replies
8.8k
views
Tutorial:
High Speed Downloading of SRA, SAM and Fastq Files
fastq
sam
Aspera-Connect
sra
updated 11 months ago by
Ram
43k • written 6.4 years ago by
Wenhu_Cao
▴ 100
6
votes
3
replies
4.5k
views
Tutorial:
Principles and Workflow of 16S/18S/ITS Amplicon Sequencing
alignment
sequencing
RNA-seq
gene
updated 13 months ago by
Ram
43k • written 5.5 years ago by
contact
▴ 40
2
votes
3
replies
2.0k
views
Tutorial:
Help demonstrate reproducibility in bioinformatics with the GA4GH's tool execution Challenge
GA4GH
Docker
CWL
Rabix
updated 12 months ago by
Ram
43k • written 7.2 years ago by
adrian.sharma
▴ 20
2
votes
3
replies
601
views
Tutorial:
Bulk RNA-seq: Different Expression Analysis with DEseq2
python
pyDESeq2
RNA-seq
updated 3 months ago by
Ram
43k • written 3 months ago by
Julia Ma
▴ 120
50
votes
3
replies
4.8k
views
Tutorial:
[[ Please read before posting a question ::: How To Ask A Good Question ]]
how-to
updated 13 months ago by
Ram
43k • written 5.3 years ago by
ATpoint
82k
0
votes
3
replies
53k
views
Tutorial:
Installation VMD on LINUX_64
gromacs
VMD
updated 13 months ago by
Ram
43k • written 7.9 years ago by
Hassan Rasouli
▴ 10
6
votes
3
replies
5.7k
views
Tutorial:
High Throughput Sequence Analysis With R And Bioconductor
bioconductor
updated 14 months ago by
Ram
43k • written 11.8 years ago by
Istvan Albert
100k
1
vote
3
replies
959
views
Tutorial:
Gibbs Sampling Algorithm Implementation for Searching DNA Motifs
montecarlo
DNA
motif
gibbssampling
splice
13 months ago by
barslmn
★ 2.1k
16
votes
3
replies
4.4k
views
Tutorial:
Methylation Analysis Tutorial in R_part2
cancer
methylation
RNA-Seq
R
updated 3.2 years ago by
zhuanggj706
• 0 • written 3.6 years ago by
Hamid Ghaedi
3.2k
19
votes
3
replies
14k
views
Tutorial:
bedGraphToBigWig Tutorial and Report
bedGraphToBigWig
updated 21 months ago by
Ram
43k • written 8.2 years ago by
Shicheng Guo
★ 9.4k
3
votes
3
replies
2.8k
views
Tutorial:
Generating count matrix for STAR counts in GDC v32.0 for RNA-Seq
GDC
TCGABiolinks
STAR
updated 14 months ago by
Ram
43k • written 2.1 years ago by
DareDevil
★ 4.3k
7
votes
3
replies
2.9k
views
Tutorial:
Prediction of Ribosomal RNA Genes Using RNAmmer Software
Annotation
rRNA
RNAmmer
Genome
updated 15 days ago by
colindaven
6.4k • written 16 months ago by
Dr.Animo
▴ 130
51
votes
3
replies
23k
views
Tutorial:
Sam File Format - Lesser Known Tips And Tricks
sam
updated 14 months ago by
Ram
43k • written 11.3 years ago by
Ya
▴ 300
7
votes
3
replies
2.6k
views
Tutorial:
Automated dbSNP lookup by rsID position, plus genome build liftover
rsid
hg19
liftover
hg38
dbsnp
updated 13 months ago by
Randy H
▴ 110 • written 21 months ago by
Kevin Blighe
87k
45
votes
3
replies
13k
views
Tutorial:
A Wet-Lab Guide to 'what I wish I had known' for Computational Biology
bioinformatics
computational-biology
updated 13 months ago by
Ram
43k • written 8.1 years ago by
Sinji
★ 3.2k
3
votes
3
replies
1.1k
views
Tutorial:
multi-omics data integration: a case study with transcriptomics and genomics mutation data
mutation
RNAseq
multiomics
updated 4 months ago by
sil_bioinfo
▴ 40 • written 8 months ago by
Ming Tommy Tang
★ 3.9k
9
votes
3
replies
4.2k
views
Tutorial:
Ht Sequence Analysis With R And Bioconductor By The Bioinformatics Center At Uc Riverside
bioconductor
updated 14 months ago by
Ram
43k • written 11.7 years ago by
Istvan Albert
100k
0
votes
3
replies
2.5k
views
Tutorial:
Automatic Primer Design
Assembly
sequence
updated 20 months ago by
Ram
43k • written 8.5 years ago by
rutial
▴ 40
11
votes
3
replies
1.4k
views
Tutorial:
Creating great documentation for bioinformatics software
Software-Documentation
updated 13 months ago by
Ram
43k • written 7.8 years ago by
venu
7.1k
10
votes
3
replies
4.5k
views
Tutorial:
Video: Alternative Splicing and Primer Design
RNA-Seq
IGB
updated 15 months ago by
Ram
43k • written 9.6 years ago by
Nowlan Freese
▴ 860
74
votes
3
replies
39k
views
Tutorial:
Revisiting the FastQC read duplication report
fastqc
updated 2.5 years ago by
Ram
43k • written 9.8 years ago by
Istvan Albert
100k
5
votes
3
replies
4.2k
views
Tutorial:
Chip Seq Review Papers
chip-seq
updated 14 months ago by
Ram
43k • written 11.4 years ago by
prtimilsina
▴ 80
16
votes
3
replies
4.7k
views
Tutorial:
NCBI blast tutorial
ncbi
blast
updated 13 months ago by
Ram
43k • written 8.0 years ago by
Eric Normandeau
11k
0
votes
2
replies
5.4k
views
Tutorial:
Install AMOS on Ubuntu 13.10
sequencing
alignment
software-error
next-gen
updated 2.5 years ago by
Ram
43k • written 9.8 years ago by
Medhat
9.7k
3
votes
2
replies
2.7k
views
Tutorial:
Continuing Education (Pipelines): Best blogs, Online Lectures, Workshops, etc..
conferences
Workshops
updated 11 months ago by
Ram
43k • written 7.0 years ago by
jnowacki
▴ 100
6
votes
2
replies
2.7k
views
Tutorial:
Tutorial Bioinformatics Static Website
website
updated 14 months ago by
Ram
43k • written 10.3 years ago by
rob234king
▴ 610
0
votes
2
replies
2.4k
views
Tutorial:
COURSE: NGS variant analysis and interpretation for translational research
CNIO
course
translational
updated 13 months ago by
Ram
43k • written 7.7 years ago by
Folder40g
▴ 190
4
votes
2
replies
2.5k
views
Tutorial:
Apply beagle5.0 to make imputation and phasing under CentOS and PBS
beagle5.0
beagle
updated 13 months ago by
Ram
43k • written 4.9 years ago by
Shicheng Guo
★ 9.4k
6
votes
2
replies
1.6k
views
Tutorial:
GSE28955 - Agilent-016436 Human miRNA Microarray 1.0
limma
GPL6955
Microarray
miRNA
Agilent
2.7 years ago by
Kevin Blighe
87k
45
votes
2
replies
37k
views
Tutorial:
Mapping Reads With Bwa And Bowtie
bowtie
bwa
updated 14 months ago by
Ram
43k • written 12.1 years ago by
MSU course 2011
▴ 540
4
votes
2
replies
3.6k
views
Tutorial:
Introduce OpenGene: an open source library for processing NGS data
NGS
sequencing
Julia
OpenGene
updated 13 months ago by
Ram
43k • written 8.2 years ago by
chen
★ 2.5k
3
votes
2
replies
8.8k
views
Tutorial:
Use fastp to preprocess FASTQ data with unique molecular identifer (UMI) integrated
FASTQ
UMI
fastp
updated 11 months ago by
Ram
43k • written 6.4 years ago by
chen
★ 2.5k
5
votes
2
replies
2.3k
views
Tutorial:
Using Bedtools Closest (closestBed) to make distance histograms of protein binding frequency near all types of pre-RNA junctions (e.g. exon-intron an…
preRNA
closestBed
bedtools-closest
bedtools
updated 13 months ago by
Ram
43k • written 5.3 years ago by
linuxborg2
▴ 10
8
votes
2
replies
6.6k
views
Tutorial:
Troubleshooting issues with SURPI pipeline for pathogen identification from complex metagenomic NGS data.
metagenomics
clinical-genomics
updated 2.2 years ago by
Ram
43k • written 9.5 years ago by
GouthamAtla
12k
7
votes
2
replies
1.7k
views
Tutorial:
A tale of two heatmap function
clustering
heatmap
updated 13 months ago by
Ram
43k • written 7.6 years ago by
Ming Tommy Tang
★ 3.9k
4
votes
2
replies
2.9k
views
Tutorial:
Building mosdepth on macOS
nim
mosdepth
updated 8 months ago by
GenoMax
142k • written 4.0 years ago by
ATpoint
82k
0
votes
2
replies
2.6k
views
Tutorial:
Webinar on 'An integrated RNA and DNA approach to unravel genetic regulation in cancer' on 25 Feb
SNP
cancer
gene-regulation
multiomics
rna-seq
updated 2.1 years ago by
Ram
43k • written 9.2 years ago by
Genomics
▴ 70
18
votes
2
replies
10.0k
views
Tutorial:
Video: RNA-Seq Alignment and Visualization using Galaxy and IGB
RNA-Seq
Galaxy
IGB
updated 2.1 years ago by
Ram
43k • written 9.5 years ago by
Nowlan Freese
▴ 860
1
vote
2
replies
3.3k
views
Tutorial:
Standalone BLAST error: UnexpectedTagClassByte
software-error
blast
updated 18 months ago by
Ram
43k • written 8.8 years ago by
Harry Detering
▴ 10
7
votes
2
replies
1.9k
views
Tutorial:
Introduction to Human Genomics for Clinical Informaticists
genome
updated 11 months ago by
Ram
43k • written 6.7 years ago by
bdolin
▴ 90
3
votes
2
replies
551
views
Tutorial:
Single-cell RNA-seq: Preprocessing: Data integration and batch correction-2
scRNA-seq
updated 3 months ago by
ATpoint
82k • written 3 months ago by
Julia Ma
▴ 120
10
votes
2
replies
3.7k
views
Tutorial:
Graph visualization with igraph in R
Visualization
igraph
Graph
R
Network
updated 11 months ago by
Ram
43k • written 7.1 years ago by
Jean-Karim Heriche
27k
6
votes
2
replies
4.2k
views
Tutorial:
GATK method for filtering vcf lines using GT values at all or multisample level.
genome
vcf
genotype
variant-filtration
updated 11 months ago by
Ram
43k • written 6.9 years ago by
kirannbishwa01
★ 1.6k
23
votes
2
replies
5.8k
views
Tutorial:
Extracting genomic feature sequences from GTF/GFF files with AGAT
extract
GTF
GFF
sequence
updated 20 months ago by
eggrandio
▴ 40 • written 3.0 years ago by
Juke34
8.6k
4
votes
2
replies
1.1k
views
Tutorial:
How to use UCSC genome browser to check the transcription factor binding at your favorite gene
UCSC
transcription
factor
ChIP-seq
9 months ago by
Ming Tommy Tang
★ 3.9k
2
votes
2
replies
2.1k
views
Tutorial:
Human mitochondrial DNA annotation in SnpEff
chrMT
SNP
snpEff
updated 13 months ago by
Ram
43k • written 3.9 years ago by
Arup Ghosh
3.2k
87
votes
2
replies
59k
views
6 follow
Tutorial:
How To Ask Good Questions On Technical And Scientific Forums
how-to
updated 14 months ago by
Ram
43k • written 10.9 years ago by
Eric Normandeau
11k
6
votes
2
replies
5.0k
views
Tutorial:
Yes .. BBMap can do that! - Part III clumpify (mark (and dedupe) duplicates without alignment), mutate (create mutant genomes) and other miscellaneou…
bbmap
5 months ago by
GenoMax
142k
14
votes
2
replies
6.5k
views
Tutorial:
ChIP-seq analysis tutorial
ChIP-Seq
updated 13 months ago by
Ram
43k • written 8.7 years ago by
Ming Tommy Tang
★ 3.9k
14
votes
2
replies
2.4k
views
Tutorial:
Exploring OpenSNP - an RMarkdown report on crowdsourced genotypes and phenotypes
SNP
R
updated 13 months ago by
Ram
43k • written 8.1 years ago by
Jeremy Leipzig
22k
411 results • Page
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Recent Votes
Answer: Design matrix Differential expression analysis
A: extract dendrogram cluster from pheatmap
extract dendrogram cluster from pheatmap
Answer: Searching for gene in multiple genomes
Comment: RNA velocity for smart-seq2 dataset
Answer: RNA velocity for smart-seq2 dataset
Answer: RNA velocity for smart-seq2 dataset
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Recent Replies
Comment: Design matrix Differential expression analysis
by
SHN
▴ 40
Thank you for your explanation. I am working on the cell lines and I am investigating the effect of the treatment on the expression level, …
Answer: Issues with Mixture file when using CIBERSORTx
by
vjanve
• 0
I ran into same issue of "duplicated gene symbol(s)": 1: In CIBERSORTxFractions(sigmatrix = sigmatrix, mixture = mixture, : 22292 dup…
Comment: creating batch colum for batch correction
by
Expert
▴ 10
Thank you for your response. These samples were collected from the same patient at different time points(day 0, 8 and 18) and these are ind…
Comment: creating batch colum for batch correction
by
ATpoint
82k
Not sure I follow. Can you first of all describe which comparisons you want to make, pretending there was no batch effect at all. Write dow…
Comment: Pindel Segmentation Fault Error
by
Ruqaiya
• 0
This the tool still problematic?
Comment: Segmentation error while using a tool
by
Ruqaiya
• 0
Thank you for the advice, i'll keep that in mind.
Comment: Segmentation error while using a tool
by
Ruqaiya
• 0
I am trying to reproduce some bioinformatics data from a research paper-[this paper][1] as i was asked to work on an MRSA bioinfo project, …
Answer: Segmentation error while using a tool
by
Mensur Dlakic
★ 27k
Most likely you didn't do anything wrong. Going by your previous post that you have only 8 GB of RAM, the most likely reason is the lack of…
Comment: Segmentation error while using a tool
by
Thanujay S
• 0
Hey @Ruqaiya! Can you please give more details on why smalt is opted here? According to what I can see, the tool has become obsolete! Why …
Comment: Extracting mutation status from MAF files - TCGA
by
Thanujay S
• 0
Hey! Can you explain the question further? What have you tried?
Comment: Downloading older version of a tool
by
Ruqaiya
• 0
I tried the 2nd option only. I got the files of older version from the author but that version isn't working on my WSL, it's showing Segmen…
Comment: filtering before dada2
by
GenoMax
142k
> I encountered a significant decrease in the percentage of merged reads. You can check with `bbmerge.sh` from BBMap suite to see how many…
Comment: HCL database download
by
GenoMax
142k
Isn't the download link available above the plot: http://bis.zju.edu.cn/HCL/data/DGE/Adult-Transverse-Colon1_dge.txt.gz
Comment: fastq screen aligner no specified.
by
GenoMax
142k
You need to change this configuration file to point it to the location where the aligner executable can be found. So as an example followi…
Answer: Design matrix Differential expression analysis
by
Gordon Smyth
★ 7.1k
The expression levels do not change but the way that you have parametrized the expression levels does change. The two design matrices give…
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