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1,000 results • Page 1 of 20
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5
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3
replies
222
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Forum: Ideal PC configurations and operating system for bioinformatics laboratory
PC
updated 2 days ago by 141k • written 3 days ago by • 0
0
votes
0
replies
161
views
Forum: Improvement on automatic annotation of single-cell RNA-seq data from the literature using LLM
annotation scRNA llm
3 days ago by yxwucq • 0
4
votes
2
replies
281
views
Forum: Bioinformatics: Where do I start
Sequencing
updated 10 days ago by 43k • written 10 days ago by ▴ 10
7
votes
7
replies
1.2k
views
Forum: Bioinformatician who was retaliated against after reporting discriminatory harassment - Please help me navigate getting out of the situation I'm in
Career advice
updated 11 days ago by ★ 2.5k • written 11 days ago by • 0
1
vote
2
replies
338
views
Forum: VCF bulk handling
bcftools SnpEff WGS VEP VCF
9 days ago by Javier • 0
16
votes
10
replies
1.0k
views
6 follow
Forum: F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 28 days ago by 5.3k • written 29 days ago by ★ 2.4k
1
vote
1
reply
218
views
Forum: Can I use this community for software development?
software-development
updated 4 weeks ago by 43k • written 4 weeks ago by • 0
5
votes
2
replies
384
views
Forum: Can questions and answers on biostars be asked only in English? Can't I use Russian for example?
language
updated 4 weeks ago by 54k • written 4 weeks ago by • 0
0
votes
0
replies
171
views
Forum: Field of Study in Computational Biology
Genomics Computational-Biology PhD
updated 5 weeks ago by 43k • written 5 weeks ago by • 0
1
vote
3
replies
473
views
Forum: Development plan for a bioinformatics database developer/coder
developer
6 weeks ago by pixie@bioinfo ★ 1.5k
7
votes
8
replies
668
views
Forum: Bioinformatics career transition
career forum
updated 6 weeks ago by ★ 16k • written 6 weeks ago by ▴ 20
4
votes
2
replies
473
views
Forum: Exploring Amino Acid Patterns in Proteins Through N-gram Analysis
protein CAFA5
6 weeks ago by Anatoly A. ▴ 10
6
votes
6
replies
759
views
Forum: You have 3 days of zero obligations and expansive resources. What are you building?
hypothetical thought-experiment
7 weeks ago by jared.andrews07 ★ 16k
0
votes
0
replies
160
views
Forum: What other data types and pipelines you would like to see from the Genomics Data Commons (GDC)
gdc tcga genomics-data-commons
updated 7 weeks ago by 43k • written 7 weeks ago by ★ 1.2k
0
votes
2
replies
367
views
Forum: What's the state of the art in bacterial whole genome alignment?
whole genome alignment mauve
7 weeks ago by Dave Carlson ★ 1.7k
31
votes
12
replies
947
views
7 follow
Forum: Nextflow and self-made pipelines opinion
nextflow pipeline data-analysis
8 weeks ago by karlaarz ▴ 90
5
votes
4
replies
551
views
Forum: Would reaching out to local labs and startups help with my career transition?
career volunteering
updated 9 weeks ago by 141k • written 9 weeks ago by ▴ 10
1
vote
2
replies
366
views
Forum: Exploring Career Reentry: Challenges and opportunities for Women After Maternity and Childcare Breaks
Maternity Career-Gap career-reentry
updated 9 weeks ago by 82k • written 9 weeks ago by ▴ 20
8
votes
7
replies
788
views
6 follow
Forum: Tower Server configuration for NGS data analysis
NGS
updated 9 weeks ago by ▴ 120 • written 12 weeks ago by • 0
1
vote
0
replies
276
views
Forum: list of bioinformatics tools in cancer genomics
mutations CNA cancer genomics LOH
updated 9 weeks ago by 161k • written 9 weeks ago by ★ 1.4k
4
votes
2
replies
371
views
Forum: bulk seq vs. sc-seq
scATAC-seq ATAC-seq scRNAseq RNAseq
updated 11 weeks ago by ★ 5.8k • written 11 weeks ago by ▴ 10
8
votes
6
replies
2.8k
views
Forum: Demonstrating Exponential Growth In Pre-School
education
written 10.2 years ago by 10k
0
votes
0
replies
199
views
Forum: tutorial for carrier screening analyses?
carrier-screening
updated 12 weeks ago by 43k • written 12 weeks ago by ▴ 20
2
votes
3
replies
433
views
Forum: My Project
Project CLI MD-Simulations Molecular-docking
12 weeks ago by Ajay A Shettigar • 0
17
votes
11
replies
981
views
Forum: How to overcome the imposter syndrome in bioinformatics?
advice
3 months ago by sil_bioinfo ▴ 40
10
votes
7
replies
2.2k
views
Forum: How to learn bioinformatics?
learning
updated 3 months ago by ★ 1.3k • written 23 months ago by ▴ 20
26
votes
27
replies
2.0k
views
10 follow
Forum: Determining computer time required
computer hpc time
updated 3 months ago by ★ 5.8k • written 3 months ago by ▴ 40
7
votes
6
replies
729
views
Forum: where to work as a bioinformatician (NGS/IA)
employment omics ia ngs
updated 3 months ago by 43k • written 3 months ago by ▴ 20
21
votes
12
replies
3.5k
views
Forum: How to Use Biostars Part-3: Formatting Text and Using GitHub Gists
meta how-to documentation
3 months ago by Ram 43k
2
votes
1
reply
415
views
Forum: Cataloguing available spatial transcriptomics tools, then linking them together into a workflow?
cytassist transcriptomics nanostring xenium visium
3 months ago by LauferVA 4.2k
49
votes
20
replies
2.0k
views
10 follow
Forum: First experiences matter
first-time tolerance
updated 3 months ago by ★ 2.6k • written 3 months ago by ★ 27k
4
votes
3
replies
757
views
Forum: How to get started in bioinformatics
students
updated 3 months ago by 43k • written 3 months ago by ▴ 10
2
votes
2
replies
578
views
Forum: Do batches contain biological variations in omics data (RNAseq or scRNAseq)?
combat single-cell omics batch rnaseq
updated 3 months ago by 43k • written 3 months ago by ▴ 40
0
votes
2
replies
3.2k
views
Forum: I’m considering a career transition from software engineering to bioinformatics. Is it a good fit for me?
career
updated 3 months ago by 141k • written 3 months ago by ▴ 10
5
votes
3
replies
602
views
Forum: Binning the rare biosphere
metagenomics soil assembly techniques binning
updated 4 months ago by ★ 27k • written 4 months ago by ▴ 20
6
votes
2
replies
658
views
Forum: how to start Bioinformatics?
basics
updated 4 months ago by ★ 27k • written 4 months ago by • 0
19
votes
5
replies
1.1k
views
6 follow
Forum: What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2023?
genomics snps survey papers
4 months ago by Raony Guimarães ★ 1.4k
4
votes
6
replies
923
views
6 follow
Forum: Bioinformatics: what do you think of it in the next 20 years?
future
updated 4 months ago by 43k • written 4 months ago by • 0
1
vote
1
reply
486
views
Forum: want to work in open source project/project in bioinformatics
project
updated 4 months ago by 43k • written 5 months ago by • 0
3
votes
5
replies
1.0k
views
Forum: Current Trends in Bioinformatics
structural-bioinformatics
updated 4 months ago by 43k • written 5 months ago by • 0
21
votes
11
replies
2.5k
views
6 follow
Forum: Rust bioinformatics projects ideas
projects scRNA-seq RNA-seq Rust
updated 5 months ago by 22k • written 13 months ago by ▴ 150
176
votes
46
replies
19k
views
26 follow
Forum: Crac: Funny And/Or Weird Names For Bioinformatics Tools
humor
5 months ago by Istvan Albert 100k
3
votes
2
replies
620
views
Forum: RNA-Seq data analysis - question
Python RNA-Seq R
updated 5 months ago by ▴ 10 • written 5 months ago by ▴ 40
0
votes
0
replies
367
views
Forum: How to learn bioinformatics online?
self-education education learning
updated 5 months ago by 43k • written 5 months ago by • 0
6
votes
5
replies
2.3k
views
Forum: Maintaning internal or private installation of Biostar or a similar Q&A site
Biostars
6 months ago by tshtatland ▴ 190
7
votes
9
replies
1.4k
views
6 follow
Forum: Mentor for a beginner bioinformatics student
project mentor
6 months ago by UT_FAN ▴ 40
0
votes
0
replies
446
views
Forum: Steps after doublet detection in single cell data
scRNA doublet
6 months ago by ishwarvh • 0
0
votes
0
replies
352
views
Forum: Automated Checks And QA
retrospective
6 months ago by Sasha ▴ 840
7
votes
13
replies
1.4k
views
Forum: How do you validate and verify your pipeline's software updates?
Validation Verification Quality-Assurance
updated 6 months ago by 43k • written 6 months ago by ▴ 10
21
votes
7
replies
11k
views
Forum: Bioinformatics short paper journal resource [software/webapps/database]
journal software webapp
updated 6 months ago by ▴ 100 • written 8.1 years ago by 11k
1,000 results • Page 1 of 20
Recent Votes
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
Comment: Convert SAM to BAM
Comment: Should I use unpaired reads from trimmomatic
Answer: Should I use unpaired reads from trimmomatic
A: Mean Variance Relationship single cell RNA-Seq Data
Answer: Blasting two protein sequences vs two nucleotide sequences
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Scholar to GenoMax 141k
Popular Question to rheab1230 ▴ 140
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Popular Question to sil_bioinfo ▴ 40
Recent Replies
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix by BioinfGuru ★ 1.7k
Hi, Just thought I'd share this code snippet here for when each file contains multiple samples. I tried using lapply (as suggested by Ram)…
Comment: Low mapping rate with Salmon by i.sudbery 19k
Take your STAR alignment and sum all the counts for each gene. You can either do this by providing an annotation to STAR, or by running fea…
Comment: How to convert plink files to Hapmap Format by bk11 ★ 2.4k
If you run your data with plink 2.0, you will have ERRCODE column in your result file showing the reason behind "NA" p-value. https://www.b…
Comment: genome assembly records not present in assembly_summary.txt by sapuizait ▴ 10
jesus its in the Genbank file and I was looking at the refseq! I m such a moron - thanks for pointing it out -sorry about that :(
Comment: ScRNA data question by bk11 ★ 2.4k
I wonder how the `Vlnplots` will look if you normalize the data using `NormalizeData()` function in `Seurat`. The flat line in your plot ar…
Comment: Low mapping rate with Salmon by GenoMax 141k
> Does that mean that the reads that are not mapped to my trascriptome are not exons/coding genes? Reads are likely aligning in regions wh…
Comment: How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File? by cmdcolin ★ 3.8k
the gencode link is broken now but here is a back up of that blogpost on archive https://web.archive.org/web/20130618221342/http://gencodeg…
Comment: Low mapping rate with Salmon by Patadu94 • 0
I'll try to make the decoy file again but I remember you also told me that it is only recommended and not mandatory for running `salmon`. I…
Comment: Low mapping rate with Salmon by Patadu94 • 0
For doing this, should I just look at the log.out.file?
Answer: Heatmap and rna-seq by pinheirofabiano ▴ 10
install.packages("pheatmap") library(pheatmap) setwd("/Users/data_analysis/results") data <- read.table(file…
Comment: Low mapping rate with Salmon by Patadu94 • 0
Thanks for the link GenoMax. But I was wondering, should not alignment and mapping have a similar rate and thus be correlated? In my case I…
Answer: Create a new bed file with all pairwise combinations between two other bed files by Pierre Lindenbaum 161k
bedtools intersect \ -a <(sort -t $'\t' -k1,1 -k2,2n A.bed) \ -b <(awk '{X=250000;P=int($2);printf("%s\t%d\…
Comment: Create a new bed file with all pairwise combinations between two other bed files by J • 0
The command lines I wrote out for you are the ones I actually used. My original reference to bedtools intersect was because I assumed the b…
Comment: ScRNA data question by starswillfade ▴ 10
I just thought it was weird that considering each dot is a cell their expressions were all on a single line instead of being spread out lik…
Comment: ScRNA data question by starswillfade ▴ 10
![enter image description here][1] I am already using Seurat V5 I think. I am using SCTransform as it was recommended by the seurat page. I…
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