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68
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22
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7.9k
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16 follow
Forum: Learning Python: list of resources
python
updated 12 months ago by 43k • written 7.5 years ago by ★ 2.8k
27
votes
22
replies
5.7k
views
8 follow
Forum: Recommendations of Linux distros for beginners in bioinformatics
training linux
updated 11 months ago by 43k • written 6.4 years ago by ▴ 810
67
votes
21
replies
6.1k
views
8 follow
Forum: Meta: Markdown in Biostars (now enabled)
meta
13 months ago by Ram 43k
81
votes
21
replies
5.1k
views
16 follow
Forum: [UPDATE] Thrown into a Bioinformatics Position
bioinformatics
updated 13 months ago by 43k • written 7.9 years ago by ★ 3.2k
27
votes
21
replies
9.3k
views
9 follow
Forum: Better for my career: Degree in computer science or Phd in bioinformatics?
computer-science phd
updated 13 months ago by 43k • written 7.6 years ago by ★ 1.7k
99
votes
21
replies
8.9k
views
14 follow
Forum: How Has Bioinformatics Improved Over Time?
bioinformatics
updated 14 months ago by 43k • written 11.3 years ago by ▴ 370
17
votes
21
replies
3.5k
views
8 follow
Forum: bioinformatics thesis
thesis
updated 3.0 years ago by 21k • written 3.1 years ago by ▴ 40
35
votes
21
replies
3.1k
views
11 follow
Forum: Should you restrict use of your software based on your political views?
software
updated 19 months ago by 43k • written 8.6 years ago by 27k
42
votes
21
replies
15k
views
10 follow
Forum: A Database Of Signatures Of Selection In The 1000 Genomes Dataset
1000genomes human selection
updated 14 months ago by 43k • written 10.2 years ago by 28k
29
votes
21
replies
6.3k
views
Forum: PhD vs Field application scientist in industry
career
updated 13 months ago by 43k • written 7.7 years ago by ▴ 80
39
votes
21
replies
14k
views
10 follow
Forum: Orientation of PE reads a review of --fr --ff and --rf meanings
RNA-Seq
updated 11 months ago by 43k • written 5.5 years ago by 8.6k
36
votes
21
replies
19k
views
9 follow
Forum: What Is Your Favorite Conserved Protein?
protein conservation
updated 14 months ago by 43k • written 10.7 years ago by 11k
66
votes
21
replies
6.8k
views
18 follow
Forum: Is there anyone who was a pure biologist?
next-gen
updated 14 months ago by 43k • written 10.0 years ago by ▴ 100
20
votes
21
replies
2.9k
views
6 follow
Forum: Next Gen seq for Job interview
next-gen
updated 13 months ago by 43k • written 9.2 years ago by ▴ 20
42
votes
20
replies
3.4k
views
11 follow
Forum: What are the most popular NGS tools?
ensembl NGS
updated 13 months ago by 43k • written 8.1 years ago by 23k
50
votes
20
replies
6.0k
views
18 follow
Forum: Explain bioinformatics to a 10 year old
bioinformatics
updated 14 months ago by 43k • written 8.2 years ago by ★ 1.1k
12
votes
20
replies
3.3k
views
Forum: Starting a career in Bioinformatics
career
updated 13 months ago by 43k • written 7.9 years ago by ▴ 10
7
votes
20
replies
2.1k
views
Forum: NGS RNA-Seq Analysis Pipeline Tophat vs STAR
STAR RNA-Seq TopHat
updated 11 months ago by 43k • written 3.3 years ago by ▴ 10
49
votes
20
replies
2.1k
views
10 follow
Forum: First experiences matter
first-time tolerance
updated 3 months ago by ★ 2.6k • written 4 months ago by ★ 27k
91
votes
20
replies
20k
views
15 follow
Forum: Publications for individualized medicine in cancer by whole genome, exome or transcriptome sequencing
N-of-one WGS RNA-Seq next-gen Exome
updated 19 months ago by 43k • written 10.0 years ago by 20k
9
votes
19
replies
8.6k
views
Forum: Careful!! Velvet Generates Different Assemblies From The Same Input Data And Same Input Parameters With Openmp Enabled.
velvet genome
updated 14 months ago by 43k • written 10.5 years ago by ▴ 660
27
votes
19
replies
4.6k
views
10 follow
Forum: Google genomics cloud platform ?
genome cloud
updated 13 months ago by 43k • written 8.9 years ago by 12k
47
votes
19
replies
14k
views
10 follow
Forum: Is Software Development a key part of Bioinformatics?
Software-Development
updated 20 months ago by 43k • written 9.3 years ago by ▴ 70
38
votes
19
replies
10k
views
10 follow
Forum: Kallisto New RNA-seq quantification method discussion
RNA-Seq
updated 13 months ago by 43k • written 8.1 years ago by ▴ 550
34
votes
19
replies
7.9k
views
12 follow
Forum: Programming Language In Bioinformatics
perl python
updated 14 months ago by 43k • written 11.0 years ago by ▴ 260
57
votes
19
replies
28k
views
6 follow
Forum: Attention: Bowtie2 And Multiple Hits
next-gen bowtie mapping
updated 14 months ago by 43k • written 11.5 years ago by 11k
70
votes
19
replies
5.6k
views
13 follow
Forum: Thrown Into A Bioinformatics Position
computational-analysis
updated 22 months ago by 43k • written 8.6 years ago by ▴ 170
30
votes
19
replies
10k
views
6 follow
Forum: Programming language use distribution from recent programs / articles
programming-language programs
updated 11 months ago by 43k • written 7.0 years ago by 11k
45
votes
19
replies
2.9k
views
9 follow
Forum: Where and how NGS techniques are heading for the next 5 years?
next-gen NGS
updated 11 months ago by 43k • written 6.9 years ago by 5.8k
49
votes
19
replies
7.3k
views
13 follow
Forum: Why Are There No Proteomics Questions In Biostar?
proteomics biostars
updated 14 months ago by 43k • written 13.5 years ago by ▴ 410
50
votes
19
replies
4.9k
views
7 follow
Forum: Proposal: Biostar Wants To Run Your Ads. Feedback Requested.
biostars
updated 14 months ago by 43k • written 11.2 years ago by 100k
56
votes
19
replies
4.1k
views
9 follow
Forum: How to apply for a job
resume cover-letter application CV
updated 11 months ago by 43k • written 6.8 years ago by 23k
35
votes
19
replies
4.0k
views
Forum: Crowd Sourcing The 'Analysis' Of The 'Big Foot' Genome
bigfoot genome
updated 14 months ago by 43k • written 11.2 years ago by 4.5k
35
votes
19
replies
3.8k
views
6 follow
Forum: For the general public seeking advice from this community
diagnosis rare-disease
updated 20 months ago by 43k • written 8.6 years ago by 11k
72
votes
18
replies
7.5k
views
17 follow
Forum: What Is One Book Every Bioinformatician Should Read?
books
updated 14 months ago by 43k • written 10.8 years ago by 8.4k
21
votes
18
replies
1.8k
views
Forum: Is it safe to keep our data in github
github
updated 10 months ago by 43k • written 4.0 years ago by ▴ 70
29
votes
18
replies
2.7k
views
12 follow
Forum: what are the big questions in bioinformatics field nowadays
genome
updated 11 months ago by 43k • written 4.2 years ago by ▴ 20
38
votes
18
replies
4.2k
views
11 follow
Forum: Should We Have A Biostar Conference Or Meeting?
conference biostars
updated 14 months ago by 43k • written 10.9 years ago by 54k
52
votes
18
replies
6.3k
views
14 follow
Forum: Do You Feel Good Or Bad About Putting Your Photo At Biostar?
meta biostars
updated 14 months ago by 43k • written 10.6 years ago by ★ 1.1k
29
votes
18
replies
3.3k
views
7 follow
Forum: Should I quit PhD program?
education career research phd
updated 11 months ago by 43k • written 6.0 years ago by ▴ 30
36
votes
18
replies
8.2k
views
Forum: How Does Genomebrowse Free Genome Browser From Golden Helix Compare?
visualization genome-browser
updated 3.0 years ago by 20 • written 11.1 years ago by ▴ 140
23
votes
18
replies
1.5k
views
Forum: Versioning of Blast DB and Reproducible Research
database blast reproducible-research
updated 10 months ago by 43k • written 4.0 years ago by ▴ 60
13
votes
18
replies
3.2k
views
6 follow
Forum: Bioinformatics independent study group
Proteomic RNA-Seq Genomic
updated 11 months ago by 43k • written 6.7 years ago by ★ 1.3k
23
votes
18
replies
2.6k
views
9 follow
Forum: How to imagine the future of bioinformatics?
bioinformatics
updated 13 months ago by 43k • written 7.6 years ago by ▴ 570
26
votes
17
replies
2.3k
views
9 follow
Forum: GalaxyProject, why and why not?
galaxy sequencing pipeline
updated 13 months ago by 43k • written 8.2 years ago by ▴ 50
21
votes
17
replies
9.2k
views
7 follow
Forum: In YOUR personal opinion, who are the top 5 bioinformatics researchers in NGS in 21st century?
next-gen
updated 12 months ago by 43k • written 7.3 years ago by ▴ 10
38
votes
17
replies
3.2k
views
7 follow
Forum: Why does bioinformatics use a special format like a BAM file instead of a database like SQLite?
sam bam samtools
updated 10 months ago by 43k • written 3.7 years ago by ▴ 250
33
votes
17
replies
3.5k
views
7 follow
Forum: What bioinformatics tools/software do not get enough recognition?
software
updated 14 months ago by 43k • written 9.5 years ago by 24k
17
votes
17
replies
2.0k
views
6 follow
Forum: Journal for beginners
journal
updated 11 months ago by 43k • written 6.1 years ago by ▴ 20
13
votes
17
replies
4.8k
views
8 follow
Forum: A Universal/Common Biorep System
reputation
updated 14 months ago by 43k • written 10.4 years ago by 20k
1,000 results • Page 3 of 20
Recent Votes
Merging a large number of VCF files
A: Merging a large number of VCF files
A: pulling snps from vcf file using ids
pulling snps from vcf file using ids
Comment: No INFO/info value in headers
how to combine multiple RNAseq count files into a single dataframe in R and unix
Comment: Greatly speed up conda by using mamba
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Recent Awards • All
Popular Question to SHN ▴ 40
Popular Question to Rob ▴ 170
Scholar to GenoMax 142k
Popular Question to curious ▴ 750
Popular Question to M. ▴ 30
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Commentator to e.r.zakiev ▴ 210
Recent Replies
Answer: The GDC Legacy Archive is retiring soon. by lcordeiro ▴ 40
Hi Zhenyu, do you happen to know how I can download metadata for some of the legacy files? I downloaded several IDATs from the legacy data …
Answer: Force PLINK2 to neglect chrX as the sex chromosome by chrchang523 10k
(cross-posted from https://groups.google.com/g/plink2-users/c/Qphx_6BpPII ) You need to use --chr-set, not --allow-extra-chr, to specify…
Answer: Easy way to find out which allele is minor allele from bed file? by chrchang523 10k
This question does not make sense. If you care about allele-order "information [being] lost in downstream applications", REF/ALT is the ob…
Answer: visualize GSEA by bk11 ★ 2.4k
I have made some edits in your code basically changing the position of text and commented out the line `data$text_pos <- ifelse(data$NES > …
Comment: Differential Expression Analysis using DESeq2 in R by swbarnes2 14k
Pictures of code are useless. You were told to paste the code and use the 0101 button. Can you explain why you decided not to do that? If…
Comment: Differential Expression Analysis using DESeq2 in R by Erina • 0
![Here is the code I am using for the analysis][1] [1]: /media/images/8a511904-6ae8-47f5-b647-ee15b02b
Answer: Clarification regarding SAM flags "mate reverse strand" (flag 16/0x10) and "read by swbarnes2 14k
In paired end sequencing, every read has a mate, and the flags all together tell you not only what is up with the read you are looking at, …
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix by Mbofire • 0
I need assistance don't know what to do next after signing up
Comment: Easy way to find out which allele is minor allele from bed file? by curious ▴ 750
According to the link: "When *generating* such filesets, PLINK 1.x defaults to swapping the alleles whenever A1's frequency is above (not …
Comment: TaxID mapping file by GenoMax 142k
nodesDB file should have been installed if you had used "Install" script for `blobtools` according to : https://blobtools.readme.io/docs/ta…
Comment: Parametric or non-parametric tests for qPCR significance testing between treatme by e.r.zakiev ▴ 210
obviously if the values are really sampled from a (quasi) normally-distributed values, then n doesn't matter, he could use t-test even with…
Comment: Converting CRAM to FastQ by Maverick ▴ 10
Understood Thank you!
Comment: Converting CRAM to FastQ by GenoMax 142k
You can use any reference that you like at this point. Since you are planning to use GATK, you can get their version from the resource bund…
Comment: Converting CRAM to FastQ by Maverick ▴ 10
![enter image description here][1]I was able to get my fastq files. I can see that my reference files are cached in a hts-ref directory but…
Comment: Differential Expression Analysis using DESeq2 in R by swbarnes2 14k
Okay, that shows us the problem, but not what we need to solve it. But, for instance, are you sure that period after the sample name shou…
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