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68
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22
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7.9k
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16 follow
Forum: Learning Python: list of resources
python
updated 12 months ago by 43k • written 7.5 years ago by ★ 2.8k
20
votes
22
replies
13k
views
11 follow
Forum: Nextseq for analysts
basespace casava bcl2fastq nextseq
updated 13 months ago by 43k • written 9.9 years ago by 5.3k
36
votes
21
replies
19k
views
9 follow
Forum: What Is Your Favorite Conserved Protein?
protein conservation
updated 14 months ago by 43k • written 10.7 years ago by 11k
81
votes
21
replies
5.1k
views
16 follow
Forum: [UPDATE] Thrown into a Bioinformatics Position
bioinformatics
updated 13 months ago by 43k • written 7.9 years ago by ★ 3.2k
27
votes
21
replies
9.2k
views
9 follow
Forum: Better for my career: Degree in computer science or Phd in bioinformatics?
computer-science phd
updated 12 months ago by 43k • written 7.6 years ago by ★ 1.7k
17
votes
21
replies
3.5k
views
8 follow
Forum: bioinformatics thesis
thesis
updated 3.0 years ago by 21k • written 3.1 years ago by ▴ 40
99
votes
21
replies
8.9k
views
14 follow
Forum: How Has Bioinformatics Improved Over Time?
bioinformatics
updated 14 months ago by 43k • written 11.3 years ago by ▴ 370
39
votes
21
replies
13k
views
10 follow
Forum: Orientation of PE reads a review of --fr --ff and --rf meanings
RNA-Seq
updated 11 months ago by 43k • written 5.5 years ago by 8.5k
20
votes
21
replies
2.9k
views
6 follow
Forum: Next Gen seq for Job interview
next-gen
updated 13 months ago by 43k • written 9.2 years ago by ▴ 20
29
votes
21
replies
6.2k
views
Forum: PhD vs Field application scientist in industry
career
updated 12 months ago by 43k • written 7.7 years ago by ▴ 80
66
votes
21
replies
6.8k
views
18 follow
Forum: Is there anyone who was a pure biologist?
next-gen
updated 13 months ago by 43k • written 10.0 years ago by ▴ 100
67
votes
21
replies
6.1k
views
8 follow
Forum: Meta: Markdown in Biostars (now enabled)
meta
13 months ago by Ram 43k
42
votes
21
replies
15k
views
10 follow
Forum: A Database Of Signatures Of Selection In The 1000 Genomes Dataset
1000genomes human selection
updated 14 months ago by 43k • written 10.2 years ago by 28k
35
votes
21
replies
3.1k
views
11 follow
Forum: Should you restrict use of your software based on your political views?
software
updated 19 months ago by 43k • written 8.6 years ago by 27k
91
votes
20
replies
20k
views
15 follow
Forum: Publications for individualized medicine in cancer by whole genome, exome or transcriptome sequencing
N-of-one WGS RNA-Seq next-gen Exome
updated 18 months ago by 43k • written 9.9 years ago by 20k
7
votes
20
replies
2.1k
views
Forum: NGS RNA-Seq Analysis Pipeline Tophat vs STAR
STAR RNA-Seq TopHat
updated 11 months ago by 43k • written 3.3 years ago by ▴ 10
12
votes
20
replies
3.3k
views
Forum: Starting a career in Bioinformatics
career
updated 13 months ago by 43k • written 7.9 years ago by ▴ 10
50
votes
20
replies
6.0k
views
18 follow
Forum: Explain bioinformatics to a 10 year old
bioinformatics
updated 13 months ago by 43k • written 8.1 years ago by ★ 1.1k
49
votes
20
replies
2.0k
views
10 follow
Forum: First experiences matter
first-time tolerance
updated 3 months ago by ★ 2.6k • written 3 months ago by ★ 27k
42
votes
20
replies
3.4k
views
11 follow
Forum: What are the most popular NGS tools?
ensembl NGS
updated 12 months ago by 43k • written 8.0 years ago by 23k
35
votes
19
replies
3.8k
views
6 follow
Forum: For the general public seeking advice from this community
diagnosis rare-disease
updated 19 months ago by 43k • written 8.6 years ago by 11k
57
votes
19
replies
28k
views
6 follow
Forum: Attention: Bowtie2 And Multiple Hits
next-gen bowtie mapping
updated 14 months ago by 43k • written 11.5 years ago by 11k
45
votes
19
replies
2.9k
views
9 follow
Forum: Where and how NGS techniques are heading for the next 5 years?
next-gen NGS
updated 11 months ago by 43k • written 6.9 years ago by 5.8k
38
votes
19
replies
10k
views
10 follow
Forum: Kallisto New RNA-seq quantification method discussion
RNA-Seq
updated 12 months ago by 43k • written 8.0 years ago by ▴ 550
56
votes
19
replies
4.1k
views
9 follow
Forum: How to apply for a job
resume cover-letter application CV
updated 11 months ago by 43k • written 6.8 years ago by 23k
49
votes
19
replies
7.3k
views
13 follow
Forum: Why Are There No Proteomics Questions In Biostar?
proteomics biostars
updated 14 months ago by 43k • written 13.5 years ago by ▴ 410
50
votes
19
replies
4.9k
views
7 follow
Forum: Proposal: Biostar Wants To Run Your Ads. Feedback Requested.
biostars
updated 14 months ago by 43k • written 11.2 years ago by 100k
30
votes
19
replies
10k
views
6 follow
Forum: Programming language use distribution from recent programs / articles
programming-language programs
updated 11 months ago by 43k • written 7.0 years ago by 11k
35
votes
19
replies
4.0k
views
Forum: Crowd Sourcing The 'Analysis' Of The 'Big Foot' Genome
bigfoot genome
updated 14 months ago by 43k • written 11.2 years ago by 4.5k
47
votes
19
replies
14k
views
10 follow
Forum: Is Software Development a key part of Bioinformatics?
Software-Development
updated 20 months ago by 43k • written 9.3 years ago by ▴ 70
9
votes
19
replies
8.6k
views
Forum: Careful!! Velvet Generates Different Assemblies From The Same Input Data And Same Input Parameters With Openmp Enabled.
velvet genome
updated 14 months ago by 43k • written 10.4 years ago by ▴ 660
27
votes
19
replies
4.6k
views
10 follow
Forum: Google genomics cloud platform ?
genome cloud
updated 13 months ago by 43k • written 8.9 years ago by 12k
34
votes
19
replies
7.9k
views
12 follow
Forum: Programming Language In Bioinformatics
perl python
updated 14 months ago by 43k • written 11.0 years ago by ▴ 260
70
votes
19
replies
5.6k
views
13 follow
Forum: Thrown Into A Bioinformatics Position
computational-analysis
updated 21 months ago by 43k • written 8.6 years ago by ▴ 170
21
votes
18
replies
1.7k
views
Forum: Is it safe to keep our data in github
github
updated 10 months ago by 43k • written 4.0 years ago by ▴ 70
72
votes
18
replies
7.5k
views
17 follow
Forum: What Is One Book Every Bioinformatician Should Read?
books
updated 14 months ago by 43k • written 10.8 years ago by 8.4k
23
votes
18
replies
2.6k
views
9 follow
Forum: How to imagine the future of bioinformatics?
bioinformatics
updated 12 months ago by 43k • written 7.6 years ago by ▴ 570
13
votes
18
replies
3.2k
views
6 follow
Forum: Bioinformatics independent study group
Proteomic RNA-Seq Genomic
updated 11 months ago by 43k • written 6.7 years ago by ★ 1.3k
29
votes
18
replies
3.3k
views
7 follow
Forum: Should I quit PhD program?
education career research phd
updated 11 months ago by 43k • written 6.0 years ago by ▴ 30
52
votes
18
replies
6.3k
views
14 follow
Forum: Do You Feel Good Or Bad About Putting Your Photo At Biostar?
meta biostars
updated 14 months ago by 43k • written 10.6 years ago by ★ 1.1k
29
votes
18
replies
2.7k
views
12 follow
Forum: what are the big questions in bioinformatics field nowadays
genome
updated 10 months ago by 43k • written 4.2 years ago by ▴ 20
36
votes
18
replies
8.1k
views
Forum: How Does Genomebrowse Free Genome Browser From Golden Helix Compare?
visualization genome-browser
updated 3.0 years ago by 20 • written 11.1 years ago by ▴ 140
38
votes
18
replies
4.2k
views
11 follow
Forum: Should We Have A Biostar Conference Or Meeting?
conference biostars
updated 14 months ago by 43k • written 10.9 years ago by 54k
23
votes
18
replies
1.5k
views
Forum: Versioning of Blast DB and Reproducible Research
database blast reproducible-research
updated 10 months ago by 43k • written 3.9 years ago by ▴ 60
21
votes
17
replies
9.2k
views
7 follow
Forum: In YOUR personal opinion, who are the top 5 bioinformatics researchers in NGS in 21st century?
next-gen
updated 12 months ago by 43k • written 7.3 years ago by ▴ 10
42
votes
17
replies
38k
views
15 follow
Forum: List Of Genome Sequencing Centers/Institutes
genomics institutes
updated 14 months ago by 43k • written 11.2 years ago by 19k
33
votes
17
replies
3.5k
views
7 follow
Forum: What bioinformatics tools/software do not get enough recognition?
software
updated 13 months ago by 43k • written 9.5 years ago by 24k
15
votes
17
replies
3.3k
views
10 follow
Forum: What does a recruiter expect from a Bioinformatician ?
expectations recruitment skills
updated 22 months ago by 43k • written 6.2 years ago by ▴ 960
13
votes
17
replies
4.8k
views
8 follow
Forum: A Universal/Common Biorep System
reputation
updated 14 months ago by 43k • written 10.4 years ago by 20k
26
votes
17
replies
2.3k
views
9 follow
Forum: GalaxyProject, why and why not?
galaxy sequencing pipeline
updated 13 months ago by 43k • written 8.2 years ago by ▴ 50
1,000 results • Page 3 of 20
Recent Votes
Comment: NGS forensics: how to know if data is fabricated
A: Hard time trying to calculate Allele Frequency and DP from Platypus
Answer: NGS forensics: how to know if data is fabricated
Answer: NGS forensics: how to know if data is fabricated
Comment: NGS forensics: how to know if data is fabricated
Answer: NGS forensics: how to know if data is fabricated
Comment: NGS forensics: how to know if data is fabricated
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Recent Replies
Answer: Finding batch and outlayers by Tigran • 0
![enter image description here][1] [1]: /media/images/aa60b9ef-b08c-4093-9f0d-fb656a5c
Comment: NGS forensics: how to know if data is fabricated by benformatics 3.9k
Can you post an IGV screenshot of what this looks like?
Comment: NGS forensics: how to know if data is fabricated by benformatics 3.9k
Yeah some of the Illumina machines pre-trim adapters (not sure if this is still the standard). I've also definitely also seen quality-trimm…
Comment: PDB related issue by noodle ▴ 530
You can typically find much more useful info in the cif file, for example what you've found is called '_atom_site.pdbx_PDB_ins_code ' and d…
Comment: PDB related issue by Mensur Dlakic ★ 27k
The best way to find out is to read the original paper. My guess is that the residue has been crystallized in alternative conformations, wh…
Answer: NGS forensics: how to know if data is fabricated by Mensur Dlakic ★ 27k
I share the opinion by @philippbayer I have seen enough questionable things in life and in science to keep me permanently jaded, but there…
Comment: How many reads for WGS Sequencing? by Mensur Dlakic ★ 27k
I downloaded the data in two files from the link you provided. With this command: megahit -1 ERR072246_1.fastq.gz -2 ERR072246_2.fastq…
Comment: How many reads for WGS Sequencing? by Mensur Dlakic ★ 27k
If you have Linux the assembly should work on your system, but 8 GB is generally not enough for assembling larger genomes.
Comment: How many reads for WGS Sequencing? by Mensur Dlakic ★ 27k
I think you might be getting stuck on less relevant parts of my exercise. The most important point was that nothing is wrong with the data.…
Comment: What does it mean single base resolution in sequencing? by dsull ★ 5.9k
Great comprehensive explanation and overview of the technologies -- with regards to sequencing assays, the one thing I'd add is SNV identif…
Answer: What does it mean single base resolution in sequencing? by dsull ★ 5.9k
I think that term means slightly different things in different contexts. For example, it's useful to profile mRNA modifications (e.g. m6a) …
Comment: Cannot process all the reads in a fast5 file? by chujie • 0
Hi, I met the same problem, have you found the solution?
Answer: What does it mean single base resolution in sequencing? by LauferVA 4.2k
Hi @fe3e6f65 , In a single sentence, the answer to your question can be summarized as, "*the resolution of a genomic technology refers to …
Comment: PCA plot by LauferVA 4.2k
Without more context, we cannot interpret this PCA plot. There are many possibilities. In addition, this question is basic enough that it w…
Comment: NGS forensics: how to know if data is fabricated by Jeremy Leipzig 22k
i have the name ready: out**liar**
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