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116,847 results • Page
1 of 2337
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0
votes
1
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198
views
Snakemake fails to find conda in PBS
snakemake
updated just now by
tim.booth
▴ 40 • written 27 days ago by
yixinzeng
• 0
0
votes
1
reply
30
views
Different output for read length
samtools
BAM
updated 10 minutes ago by
Pierre Lindenbaum
161k • written 1 hour ago by
marco.barr
▴ 100
0
votes
2
replies
35
views
Presence of unknown sites in ANNOVAR output file
ANNOVAR
12 minutes ago by
sainavyav22
• 0
0
votes
0
replies
16
views
Truncated metadata file report from ENA Portal API
ena
python
updated 59 minutes ago by
Ram
43k • written 1 hour ago by
Giulia
• 0
795
votes
167
replies
143k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 3 months ago by
Biostar
2.7k • written 7.4 years ago by
Istvan Albert
100k
0
votes
0
replies
15
views
What is workflow for de-novo assembling of nuclear and mito genomes of non-model organisms
de-novo
WGS
DNA-seq
assembling
1 hour ago by
Matvii Mykhailichenko
• 0
0
votes
3
replies
432
views
JASPAR2024_getMatrixSet error
JASPAR2024
getMatrixSet
updated 2 hours ago by
Ram
43k • written 11 weeks ago by
maplewj
▴ 10
0
votes
3
replies
86
views
How to convert normalized BigWig file to count matrix?
count-matrix
BigWig
updated 2 hours ago by
Ram
43k • written 4 hours ago by
feather-W
• 0
0
votes
3
replies
156
views
bcftools - reducing to "sites-only"?
sites-only
bcftools
updated 3 hours ago by
Pierre Lindenbaum
161k • written 18 hours ago by
Matthew
• 0
3
votes
4
replies
2.9k
views
Have you ever obtained user guide of ExomeCNV?
CNV
updated 4.4 years ago by
lffu_0032
▴ 90 • written 5.2 years ago by
oghzzang
▴ 50
0
votes
1
reply
39
views
input file for alternative splicing in rmats in linux
rmats
updated 1 hour ago by
Ram
43k • written 4 hours ago by
Lambodarswain316
• 0
0
votes
2
replies
116
views
Server or aws cloud- which one is better for gatk pipeline
aws
server
calling
gatk
cloud
updated 5 hours ago by
Michael
54k • written 11 hours ago by
ashaneev07
▴ 20
1
vote
1
reply
366
views
ComBat_Seq stuck adjusting the data
Batch-Effect
RNAseq
ComBat-Seq
updated 5 hours ago by
Jaïr
• 0 • written 4 months ago by
NorbertK
▴ 10
4
votes
3
replies
541
views
How to solve the error 'ERROR::MATE_NOT_FOUND:Found xxx unpaired mates' when run SamToFastq
samtofasq
picard
validatesamfile
updated 5 hours ago by
Pierre Lindenbaum
161k • written 7 hours ago by
Lila M
★ 1.2k
1
vote
3
replies
109
views
Nextflow: how to get the process work dir, within the process
nextflow
updated 5 hours ago by
Pierre Lindenbaum
161k • written 6 hours ago by
ScottDansk
▴ 10
1
vote
0
replies
62
views
What is Deepvariant default filtering values ?
vcf
calling
filter
variant
deepvariant
7 hours ago by
Shae
▴ 10
0
votes
1
reply
124
views
Enhanced Volcano-Cut off
foldchange
enhancedvolcano
updated 8 hours ago by
marco.barr
▴ 100 • written 18 hours ago by
odi
▴ 10
1
vote
4
replies
169
views
Importing a fastq file
Fastq
9 hours ago by
oumo
• 0
0
votes
6
replies
2.1k
views
Copy number Variation analysis using conumee
R
software error
sequencing
genome
updated 5 hours ago by
GenoMax
142k • written 3.2 years ago by
gs000095
▴ 10
1
vote
1
reply
89
views
Conda severely broken after attempting mamba install
biopython
conda
mamba
pycosat
updated 9 hours ago by
andres.firrincieli
3.6k • written 10 hours ago by
kacollier
▴ 30
1
vote
2
replies
1.4k
views
Seurat CellCycleScoring – confused about the proper order of operations when using SCTransform
CellCycleScoring
SCTransform
Seurat
updated 12 hours ago by
Li
• 0 • written 2.3 years ago by
GPM
▴ 10
0
votes
0
replies
64
views
Summary Statistics SNPs not found in Target data
SNP
plink
PRS
updated 13 hours ago by
Ram
43k • written 14 hours ago by
curious_butterfly
• 0
0
votes
0
replies
72
views
Long reads and fixing of mate-pair issues/marking duplicates with samtools
mate-pair
samtools
alignment
7 hours ago by
Zeng Hao
▴ 40
0
votes
2
replies
127
views
Contig assembly task, errors
genetics
contig
assembly
dna
bioinformatics
1 hour ago by
rackbersingh
• 0
1
vote
3
replies
126
views
Help with Biopython for Beginner
Python
ORF
FASTA
Biopython
updated 30 minutes ago by
GenoMax
142k • written 18 hours ago by
cput
• 0
0
votes
0
replies
79
views
Annotate VCF via VEP for gnomAD annotation considering FILTER column
vep
vcf
gnomad
18 hours ago by
asalimih
▴ 60
0
votes
5
replies
316
views
Filtering Multi-sample VCF file for all except one Genotype
variant
SNP
VCF
updated 18 minutes ago by
Jeremy Leipzig
22k • written 2 days ago by
schmince
• 0
0
votes
2
replies
191
views
featureCounts output summary assigned value higher than uniquely mapped reads from HISAT2
RNA-seq
featureCounts
HISAT
updated 6 hours ago by
i.sudbery
19k • written 20 hours ago by
Prawesh
• 0
0
votes
9
replies
1.5k
views
kinannote software installation problems
kinannote
updated 20 hours ago by
Emanoelle
• 0 • written 5.7 years ago by
Elizabeth
▴ 30
0
votes
1
reply
133
views
Obtaining Infinium Methylation450K manifest for hg38
TCGA
hg38
methylation
Illumina
updated 20 hours ago by
marco.barr
▴ 100 • written 23 hours ago by
elisheva
▴ 120
0
votes
0
replies
72
views
Pathway analysis of Bacteria from denovo genome assembly
KEGG-KASS
WGS
Pathway
denovo
updated 19 hours ago by
Ram
43k • written 23 hours ago by
mathavanbioinfo
▴ 90
0
votes
1
reply
222
views
Seurat V5 integration
Seurat
integration
updated 19 hours ago by
Ram
43k • written 4 days ago by
starswillfade
▴ 10
0
votes
2
replies
182
views
Error in plotting PCA plot in seurat
PCA
single-cell
seurat
scRNA
RNA
updated 19 hours ago by
Ram
43k • written 1 day ago by
Sumeet
• 0
0
votes
0
replies
89
views
News:
Online course: Introduction to Deep Learning
Python
Deep-Learning
Convolutional-Neural-Network
Machine-Learning
updated 19 hours ago by
Ram
43k • written 1 day ago by
carlopecoraro2
★ 2.5k
4
votes
5
replies
3.4k
views
How to compare compare two Sam files to check mapping
alignment
updated 1 day ago by
DavidStreid
▴ 90 • written 6.4 years ago by
madhu.9124
▴ 60
0
votes
2
replies
126
views
How/where can I get fungal mitogenome data for analysis?
fungal
mitogenome
updated 1 day ago by
GenoMax
142k • written 1 day ago by
madders73
• 0
4
votes
2
replies
222
views
How can I analyze normalized expression data?
DEseq2
RNA-seq
14 hours ago by
mnx0723
• 0
3
votes
4
replies
4.0k
views
FarmCPU - how to explain the reported 'effect'?
gwas
updated 1 day ago by
chloek88
• 0 • written 5.5 years ago by
Philipp Bayer
8.4k
0
votes
3
replies
224
views
RNA seq analysis
DESeq
RNA-seq
updated 20 hours ago by
Ram
43k • written 1 day ago by
prifa
• 0
10
votes
3
replies
269
views
TFs and Gene databases
Transcription-factor
Target
Regulatory-Networks
updated 1 day ago by
b.contreras.moreira
▴ 180 • written 1 day ago by
ijarne
• 0
0
votes
0
replies
119
views
Chance for trained without experience
bio
updated 1 day ago by
ATpoint
82k • written 1 day ago by
shehab
• 0
1
vote
2
replies
158
views
How do I change the title font size in ViolinPlot (Seurat)
Seurat
Violin-Plot
ggplot2
patchwork
1 day ago by
Assa Yeroslaviz
★ 1.8k
0
votes
0
replies
95
views
Xenium multi sample data
batch
annotation
cell
Xenium
effect
1 day ago by
Omics data mining
▴ 260
0
votes
2
replies
148
views
Phasing a mixture of two individuals' DNA with long reads
long-reads
phasing
1 day ago by
njornet
▴ 20
0
votes
2
replies
177
views
Exploring Diet Effects in Single-Cell RNA Sequencing
batch_effect
scRNA
seurat
integration
updated 1 day ago by
Adam
▴ 30 • written 1 day ago by
Darya
• 0
0
votes
5
replies
215
views
Percentage coverage of reference genome by de novo genome
SAMtools
BWA
alignment
ddRAD
updated 1 day ago by
Joe
21k • written 1 day ago by
Lemonhope
• 0
0
votes
0
replies
134
views
Why does my Hi-C contact map show large regions making little to no contact?
BWA-MEM2
Hi-C
PretextMap
9 hours ago by
Winter
• 0
0
votes
5
replies
260
views
A question about reference genome for creating the consensus sequence
fasta
fa
cram
genome
1 day ago by
me
• 0
2
votes
11
replies
2.6k
views
6 follow
CNVKIT - unable to produce scatter and diagram pdfs
cnvkit
updated 1 day ago by
DGTool
• 0 • written 4.8 years ago by
ww22runner
▴ 60
2
votes
1
reply
121
views
java out of memory error through beagle for imutation
beagle
java.out.of.memory
imputation
1 day ago by
analyst
▴ 50
116,847 results • Page
1 of 2337
Recent Votes
Answer: How to solve the error 'ERROR::MATE_NOT_FOUND:Found xxx unpaired mates' when ru
A: Insert Size And Fragment Size ?
Answer: samtools 1.0 'index' : CSI index vs BAI index ?
Comment: Nextflow and self-made pipelines opinion
Comment: Nextflow and self-made pipelines opinion
What is Deepvariant default filtering values ?
Answer: Nextflow: how to get the process work dir, within the process
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Comment: absolute path for symbolic links in Snakefile
by
tim.booth
▴ 40
Further to this answer - if you are using GNU coreutils (ie. any modern Linux), there is a "-r" flag to fix this problem. I typically use "…
Answer: Different output for read length
by
Pierre Lindenbaum
161k
I supect your reads are hard clipped. Show us the first lines of samtools view /home/sorted.bam | cut -f 2,6,10 | head -n 20
Comment: Presence of unknown sites in ANNOVAR output file
by
sainavyav22
• 0
Here you go. ***1.perl convert2annovar.pl -format vcf4old 1008_Tumor.vcf > 1008Tumor_variant.avinput 2.perl annotate_variation.pl 1008Tumo…
Comment: Filtering Multi-sample VCF file for all except one Genotype
by
Jeremy Leipzig
22k
Those are samples. If a sample has a 0/1 or a 1/1 genotype for that variant, they have the variant. Your question is ambiguous because you…
Comment: Help with Biopython for Beginner
by
GenoMax
142k
> That turns it into a mess of paragraphs See above. Select (highlight with mouse) the part you want to represent as `code` and then click…
Comment: Help with Biopython for Beginner
by
cput
• 0
That turns it into a mess of paragraphs, but if it truly is more productive thanks for the tip!
Comment: Presence of unknown sites in ANNOVAR output file
by
Ram
43k
Please show us your full annovar command line.
Comment: input file for alternative splicing in rmats in linux
by
Ram
43k
I've done what I can to make your post more professional. Stick to outlining your problem, personal pleas do not really belong on a profess…
Comment: Contig assembly task, errors
by
rackbersingh
• 0
Hi Phillip, Thanks for providing an answer and clearing up some confusion, looking into what you said about older assemblers reverse comp…
Comment: How to analyze Infinium Mouse Methylation BeadChip array data?
by
Tawny
▴ 180
Did you get this figured out? I am having the same error in R.
Answer: JASPAR2024_getMatrixSet error
by
Raghad
• 0
Try this: ```r #Read the motifs library(JASPAR2024) library(TFBSTools) jaspar <- JASPAR2024() sq24 <- RSQLite::dbConnect(RSQLite::SQLite…
Answer: How to convert normalized BigWig file to count matrix?
by
ATpoint
82k
It's not possible. Gene counts are a region aggregate, bigwigs are a per-base readout and you cannot use normalized counts for mentioned to…
Comment: bcftools - reducing to "sites-only"?
by
Pierre Lindenbaum
161k
> Don't forget to follow up on your threads. If an answer was helpful, you should upvote it; if the answer resolved your question, you shou…
Comment: bcftools - reducing to "sites-only"?
by
Matthew
• 0
Thank you Pierre! Not sure why I didn't notice that (I should have)
Comment: How to convert normalized BigWig file to count matrix?
by
Pierre Lindenbaum
161k
bigwig is chrom/start/end/value . what kind of matrix do you want ?
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