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1.2k
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205
replies
49k
views
107 follow
Forum: What Are The Most Common Stupid Mistakes In Bioinformatics?
software
updated 4 months ago by ▴ 250 • written 12.2 years ago by 21k
361
votes
57
replies
60k
views
43 follow
Forum: Recommend Your Favorite Bioinformatics Books
books education
updated 8 months ago by 85k • written 13.2 years ago by 98k
306
votes
65
replies
20k
views
48 follow
Forum: Your Favorite Bioinformatics Blogs (March 2010)
subjective
updated 8 months ago by 85k • written 13.2 years ago by 27k
294
votes
27
replies
42k
views
33 follow
Forum: Up-to-date RNA-Seq Analysis Training/Courses/Papers (Dec 2017)
RNA-Seq sequencing papers
updated 10 months ago by 39k • written 7.3 years ago by ★ 2.4k
290
votes
55
replies
14k
views
41 follow
Forum: What Do You Waste Your Time On
scripting
updated 3 months ago by 39k • written 10.1 years ago by 9.6k
286
votes
64
replies
18k
views
44 follow
Forum: Bioinformatics "Cheat Sheet"
cheat sheet
updated 8 months ago by 85k • written 12.2 years ago by 22k
279
votes
69
replies
18k
views
23 follow
Forum: Is Bioinformatics On Decline? Only In The Web, Or Also Within Institutions?
subjective
updated 3 months ago by 39k • written 11.8 years ago by ▴ 160
262
votes
48
replies
21k
views
37 follow
Forum: Bioinformatics courses, workshops or training
Workshop Training Courses
updated 12 weeks ago by ▴ 970 • written 8.0 years ago by ★ 4.2k
251
votes
36
replies
31k
views
27 follow
Forum: How Do You Manage Your Files & Directories For Your Projects ?
project-management file-management
updated 9 weeks ago by 39k • written 13.1 years ago by 154k
244
votes
36
replies
8.0k
views
32 follow
Forum: Good Habit for Bioinformatics Analyst or Scientist
Analyst Scientist
updated 7 weeks ago by 39k • written 7.1 years ago by ★ 9.3k
206
votes
58
replies
38k
views
31 follow
Forum: Will Python Take The Place Of R?
r statistics python biopython
updated 3 months ago by 39k • written 10.0 years ago by 9.6k
202
votes
41
replies
23k
views
20 follow
Forum: Genomics is not Special. Computational Biologists are reinventing the wheel for big data biology analysis
genomics cram gatk galaxy
updated 3 months ago by 39k • written 8.5 years ago by ★ 5.2k
170
votes
43
replies
16k
views
25 follow
Forum: Crac: Funny And/Or Weird Names For Bioinformatics Tools
humor
updated 4 weeks ago by 11k • written 10.2 years ago by 98k
160
votes
22
replies
16k
views
19 follow
Forum: Advice For Newcomers To The Bioinformatics Field
advice career
updated 6 months ago by 39k • written 9.9 years ago by 9.6k
151
votes
39
replies
23k
views
20 follow
Forum: A Farewell To Bioinformatics
bioinformatics
updated 3 months ago by 39k • written 10.3 years ago by 3.0k
145
votes
56
replies
7.5k
views
17 follow
Forum: Suggesting The Removal Of Post Closing
biostars
updated 3 months ago by 39k • written 10.2 years ago by 98k
144
votes
62
replies
9.6k
views
23 follow
Forum: Is The Biostar Fading? (Updated For 2014)
meta biostars
updated 3 months ago by 39k • written 12.2 years ago by ★ 2.9k
138
votes
42
replies
20k
views
22 follow
Forum: Are We Rude/Do We Expect Too Much From People Asking Questions On This Forum?
meta
updated 8 weeks ago by ★ 2.8k • written 10.0 years ago by ★ 1.6k
134
votes
65
replies
3.9k
views
11 follow
Forum: Bioinformatics vs. the Climate Crisis
RNA-Seq Climate Forum
updated 15 months ago by 52k • written 3.5 years ago by ▴ 880
129
votes
38
replies
5.9k
views
21 follow
Forum: Who are the Greatest Bioinformaticians Of All Time (GBOAT)
bioinformatics Forum
updated 4.1 years ago by 35k • written 4.1 years ago by 98k
126
votes
44
replies
28k
views
16 follow
Forum: Trimming adapter sequences - is it necessary?
NGS adapter RNA-Seq
updated 11 weeks ago by 39k • written 6.7 years ago by ▴ 970
125
votes
46
replies
44k
views
20 follow
Forum: Visualization of ChIP-seq data using Heatmaps (Updated: 06/10/16)
visualization ChIP-Seq homer ngsplot deeptools
updated 7 weeks ago by 39k • written 7.2 years ago by ★ 3.2k
120
votes
33
replies
36k
views
24 follow
Forum: Where To Look For Quality Bioinformatics Short Courses And Workshops?
Courses
updated 19 days ago by ★ 2.3k • written 9.8 years ago by 11k
116
votes
35
replies
7.1k
views
18 follow
Forum: Is Biostar Killing The Bioinformatics Core?
core biostars
updated 3 months ago by 39k • written 10.1 years ago by 21k
115
votes
35
replies
38k
views
20 follow
Forum: Snakemake vs. Nextflow: strengths and weaknesses
snakemake nextflow
updated 15 days ago by 39k • written 5.9 years ago by ▴ 760
113
votes
53
replies
5.2k
views
16 follow
Forum: Middle author, but not first author projects. How would you approach this?
career development authorship Forum
updated 4.1 years ago by ▴ 270 • written 4.1 years ago by ▴ 170
106
votes
23
replies
20k
views
16 follow
Forum: How Did You Learn Good Practice In Bioinformatics/Computational Biology?
bioinformatics
updated 12 weeks ago by 39k • written 9.3 years ago by ▴ 580
105
votes
46
replies
12k
views
13 follow
Forum: Why Does Biostar Cover Questions On Epigenetics, But Not Intelligent Design?
epigenetics
updated 12 weeks ago by 39k • written 9.7 years ago by ★ 1.1k
100
votes
38
replies
28k
views
34 follow
Forum: List of cloud genomics companies
cloud-genomics
updated 19 days ago by 21k • written 9.5 years ago by ★ 3.6k
99
votes
21
replies
8.0k
views
14 follow
Forum: How Has Bioinformatics Improved Over Time?
bioinformatics
updated 3 months ago by 39k • written 10.3 years ago by ▴ 370
98
votes
41
replies
4.6k
views
17 follow
Forum: I am really pissed off by the bioinformatics software world. Do/can we have a better solution?
software-error next-gen-sequencing
updated 7 weeks ago by 39k • written 6.7 years ago by ▴ 500
93
votes
50
replies
14k
views
17 follow
Forum: Fda Sends A Warning Letter To 23Andme - Personal Genomics Service Marketing To Be Discontinued
personal-genomics 23andMe
updated 12 weeks ago by 39k • written 9.5 years ago by 98k
91
votes
20
replies
19k
views
15 follow
Forum: Publications for individualized medicine in cancer by whole genome, exome or transcriptome sequencing
N-of-one WGS RNA-Seq next-gen Exome
updated 7 months ago by 39k • written 9.0 years ago by 20k
88
votes
32
replies
8.3k
views
11 follow
Forum: Vintage / unconventional pictures for Bioinformatics
history picture
updated 14 months ago by 39k • written 8.6 years ago by 154k
88
votes
24
replies
4.9k
views
10 follow
Forum: How To Be Helpful As A Biostar Moderator/Editor?
biostars
updated 3 months ago by 39k • written 10.3 years ago by 1.9k
86
votes
29
replies
8.7k
views
9 follow
Forum: What is the future of EMBL-EBI (and European bioinformatics in general) in case of a 'Brexit'?
EU brexit EBI politics
updated 7 weeks ago by 39k • written 7.0 years ago by 52k
84
votes
22
replies
3.6k
views
12 follow
Forum: is fortran still used in bioinformatics?
FORTRAN R
updated 11 weeks ago by 39k • written 3.9 years ago by ▴ 180
84
votes
36
replies
17k
views
13 follow
Forum: Benchmark - Comparison of Different NGS Mappers
mRNA-Seq benchmark NGS-mapper
updated 14 months ago by 39k • written 8.4 years ago by 9.9k
83
votes
30
replies
5.6k
views
18 follow
Forum: Optimal design for bioinformatics "lab" space?
design lab-space
updated 11 months ago by 39k • written 8.1 years ago by 18k
82
votes
32
replies
6.6k
views
13 follow
Forum: Shall We Go Back To Stackexchange?
meta
updated 3 months ago by 39k • written 10.5 years ago by ★ 3.6k
81
votes
21
replies
4.2k
views
16 follow
Forum: [UPDATE] Thrown into a Bioinformatics Position
bioinformatics
updated 10 weeks ago by 39k • written 7.0 years ago by ★ 3.2k
80
votes
23
replies
4.5k
views
11 follow
Forum: Are we facing a bioinformatician shortage?
salary career
updated 11 weeks ago by 39k • written 8.6 years ago by ★ 3.0k
80
votes
34
replies
4.1k
views
11 follow
Forum: Authorship issues- how to resolve?
authorship
updated 4 months ago by 39k • written 8.0 years ago by ▴ 520
79
votes
34
replies
8.0k
views
15 follow
Forum: Do bioinformaticians often break molecular biologists' hearts by being the first author?
authorship career
updated 8 weeks ago by 39k • written 7.5 years ago by ▴ 650
78
votes
31
replies
5.8k
views
14 follow
Forum: Do Bad Questions Even Exist?
biostars general education
updated 3 months ago by 39k • written 12.2 years ago by 10k
74
votes
14
replies
28k
views
12 follow
Forum: Bioinformatics Consulting Thoughts
consulting
updated 11 months ago by 39k • written 13.0 years ago by 4.5k
72
votes
28
replies
5.4k
views
10 follow
Forum: Bioinformatics commercial services as a risk factor for scientific misconduct
commercial-services ethics misconduct
updated 11 months ago by 39k • written 8.3 years ago by ▴ 90
72
votes
18
replies
6.8k
views
17 follow
Forum: What Is One Book Every Bioinformatician Should Read?
books
updated 12 weeks ago by 39k • written 9.9 years ago by 7.7k
72
votes
14
replies
3.4k
views
12 follow
Forum: Things you wish you knew when entering the bioinformatics field
pitfalls advice forum Forum
updated 2.7 years ago by 7.7k • written 2.7 years ago by ★ 14k
70
votes
19
replies
4.7k
views
13 follow
Forum: Thrown Into A Bioinformatics Position
computational-analysis
updated 10 months ago by 39k • written 7.6 years ago by ▴ 170
1,000 results • Page 1 of 20
Recent Votes
Answer: IGV displays genomic coordinates in a 1-based system or 0-based?
RNAseq expression data log2 transformed has negative values.
Samtools: "Too Many Open Files"
Filtering bad quality probes in illumina microarray data
gene filtering for agilent microarray using Limma
A: Identification Of Significant Differences Between Phylogentic Trees Using Distan
A: How to filter vcf file on minimum genotype depth and quality for each sample
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Popular Question to Sam ▴ 150
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Recent Replies
Answer: Counting intronic reads in bulk RNA-seq by swbarnes2 13k
Are you sure this is appropriate for bulk RNASeq? I thought intronic reads for single cell were only counted in nuclear preps.
Answer: Single cell chemistry by swbarnes2 13k
Are you sure these samples are 10x? How likey is it that the sequencing group messed up and ran the samples wrongly?
Comment: How to deal with duplicated gene IDs in TCGA RNA expression data? by Vincent Laufer ★ 2.8k
are they distinct transcript IDs but the same gene ID? can you provide any additional context?
Comment: SyntaxError (Perhaps you forgot a comma?) in snakefile when running FastQC. by Dhatri Badri • 0
that didn't work either
Answer: Why are some WES VCFs larger than others? by Vincent Laufer ★ 2.8k
Hi @kermit and prash (and others)! Nice discussion. I wanted to offer some **biological rationales** in addition to the technical reasons …
Answer: SyntaxError (Perhaps you forgot a comma?) in snakefile when running FastQC. by WouterDeCoster 47k
There is no `:` after `rule fastqc`
Comment: SyntaxError (Perhaps you forgot a comma?) in snakefile when running FastQC. by Dhatri Badri • 0
sorry that was a typo while i was copying my code! I still get the same error
Comment: too many zeros in 16S rRNA amplicon sequencing data by andres.firrincieli 3.3k
You have too many ASV (40k) and I think this is a problem related to the denoising (DADA2) of NovaSeq 6000 sequencing data. Maybe this post…
Answer: SyntaxError (Perhaps you forgot a comma?) in snakefile when running FastQC. by Pierre Lindenbaum 154k
> fastq.gz". what is the dot after `fastq.gz" ` ?
Comment: DownsampleSam by Pierre Lindenbaum 154k
1) I think this is just a warning 2) there must be another error displayed 3) look at samtools view --subsample 4) don't use loops but use…
Comment: ABySS genome assembler by npavliukovec • 0
Hello, yes, I tried it, but I have to do my home work on university's remote computer, unfortunatelly I go an error, when I try to use abys…
Comment: desgin a primer for CDS by yuxiang • 0
how can you know the primer is not bind to other gene site? how can you desgin an experiment? I use the FISH to identify the gene, how abo…
Comment: desgin a primer for CDS by yuxiang • 0
sorry for my late reply, I am a student, my profossor ask me to dsgin a primer for the specific cDNA, I have solved now, thanks for your re…
Comment: vg: Mapping fewer reads to a genome graph than a linear reference by samuel.a.odonnell ▴ 470
In this case filtering for quality might help correct for this overattributing feature?
Comment: ABySS genome assembler by GenoMax 129k
Did you see this guidance from ABySS manual: https://github.com/bcgsc/abyss#optimizing-the-parameters-k-and-kc
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