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Forum: What Are The Most Common Stupid Mistakes In Bioinformatics?
software Forum
updated 10 months ago by 10k • written 10.0 years ago by 20k
281
votes
54
replies
10k
views
39 follow
Forum: What Do You Waste Your Time On
bioinformatics scripting Forum
updated 4 days ago by ▴ 170 • written 7.9 years ago by 8.6k
279
votes
69
replies
15k
views
23 follow
Forum: Is Bioinformatics On Decline? Only In The Web, Or Also Within Institutions?
subjective Forum
updated 5.8 years ago by ▴ 10 • written 9.7 years ago by ▴ 160
274
votes
26
replies
34k
views
31 follow
Forum: Up-to-date RNA-Seq Analysis Training/Courses/Papers (Dec 2017)
RNA-Seq sequencing Forum papers Tutorial
3.4 years ago by CandiceChuDVM ★ 2.2k
255
votes
47
replies
17k
views
35 follow
Forum: Bioinformatics courses, workshops or training
Training Workshop Tutorial Courses Forum
2.9 years ago by Deepak Tanwar ★ 4.1k
243
votes
36
replies
6.5k
views
32 follow
Forum: Good Habit for Bioinformatics Analyst or Scientist
Habit Analyst Scientist Forum
4.2 years ago by Shicheng Guo ★ 8.6k
202
votes
41
replies
21k
views
20 follow
Forum: Genomics is not Special. Computational Biologists are reinventing the wheel for big data biology analysis
genomics Forum cram gatk galaxy
updated 6.4 years ago by 87k • written 6.4 years ago by ★ 4.8k
201
votes
55
replies
35k
views
30 follow
Forum: Will Python Take The Place Of R?
r python biopython statistics Forum
updated 3.4 years ago by 9.2k • written 7.9 years ago by 8.9k
170
votes
88
replies
12k
views
23 follow
Forum: Petition to demand that the Federal German Government release all genomic data on SARS-COV-2
GISAID Forum
written 13 months ago by 87k
156
votes
21
replies
14k
views
17 follow
Forum: Advice For Newcomers To The Bioinformatics Field
bioinformatics Forum
updated 2.8 years ago by • 0 • written 7.8 years ago by 8.9k
148
votes
39
replies
19k
views
20 follow
Forum: A Farewell To Bioinformatics
bioinformatics Forum
updated 3.5 years ago by 72k • written 8.2 years ago by 3.0k
145
votes
56
replies
6.4k
views
17 follow
Forum: Suggesting The Removal Of Post Closing
biostar Forum
updated 7.9 years ago by 49k • written 8.0 years ago by 87k
144
votes
62
replies
7.8k
views
23 follow
Forum: Is The Biostar Fading? (Updated For 2014)
biostar meta statistics Forum
updated 3.8 years ago by 48k • written 10.1 years ago by ★ 2.9k
140
votes
38
replies
11k
views
20 follow
Forum: Crac: Funny And/Or Weird Names For Bioinformatics Tools
bioinformatics Forum humor
updated 5.5 years ago by • 0 • written 8.0 years ago by 87k
135
votes
41
replies
18k
views
20 follow
Forum: Are We Rude/Do We Expect Too Much From People Asking Questions On This Forum?
Forum
updated 7.5 years ago by ★ 1.1k • written 7.9 years ago by ★ 1.5k
129
votes
38
replies
3.4k
views
21 follow
Forum: Who are the Greatest Bioinformaticians Of All Time (GBOAT)
bioinformatics Forum
updated 2.0 years ago by 32k • written 2.0 years ago by 87k
128
votes
65
replies
1.6k
views
11 follow
Forum: Bioinformatics vs. the Climate Crisis
RNA-Seq Climate Forum
updated 16 months ago by 19k • written 16 months ago by ▴ 790
121
votes
44
replies
21k
views
16 follow
Forum: Trimming adapter sequences - is it necessary?
RNA-Seq NGS adapter Forum
written 4.6 years ago by ▴ 780
119
votes
45
replies
33k
views
19 follow
Forum: Visualization of ChIP-seq data using Heatmaps (Updated: 06/10/16)
ChIP-Seq visualization deeptools ngsplot homer Forum
updated 15 months ago by ▴ 230 • written 5.1 years ago by ★ 3.1k
116
votes
35
replies
5.7k
views
18 follow
Forum: Is Biostar Killing The Bioinformatics Core?
biostar Forum core
updated 2.0 years ago by 12k • written 8.0 years ago by 20k
113
votes
31
replies
31k
views
23 follow
Forum: Where To Look For Quality Bioinformatics Short Courses And Workshops?
bioinformatics Forum
updated 2.2 years ago by • 0 • written 7.6 years ago by 11k
113
votes
53
replies
2.9k
views
16 follow
Forum: Middle author, but not first author projects. How would you approach this?
career development authorship Forum
updated 2.0 years ago by ▴ 270 • written 2.0 years ago by ▴ 170
105
votes
46
replies
9.3k
views
13 follow
Forum: Why Does Biostar Cover Questions On Epigenetics, But Not Intelligent Design?
Forum
written 7.6 years ago by ★ 1.1k
99
votes
21
replies
6.9k
views
14 follow
Forum: How Has Bioinformatics Improved Over Time?
Forum
updated 6.9 years ago by 3.4k • written 8.2 years ago by ▴ 370
99
votes
36
replies
21k
views
30 follow
Forum: List of cloud genomics companies
cloud genomics Forum
updated 3 months ago by • 0 • written 7.4 years ago by ★ 3.5k
98
votes
41
replies
3.2k
views
17 follow
Forum: I am really pissed off by the bioinformatics software world. Do/can we have a better solution?
software error sequencing next-gen Forum
updated 4.6 years ago by 87k • written 4.6 years ago by ▴ 480
95
votes
34
replies
21k
views
18 follow
Forum: Snakemake vs. Nextflow: strengths and weaknesses
snakemake nextflow Forum
updated 20 months ago by ★ 8.6k • written 3.8 years ago by ▴ 710
93
votes
50
replies
13k
views
17 follow
Forum: Fda Sends A Warning Letter To 23Andme - Personal Genomics Service Marketing To Be Discontinued
Forum
updated 7.4 years ago by ▴ 350 • written 7.4 years ago by 87k
91
votes
20
replies
17k
views
15 follow
Forum: Publications for individualized medicine in cancer by whole genome, exome or transcriptome sequencing
RNA-Seq next-gen WGS Exome N-of-one Forum
3.6 years ago by Obi Griffith 19k
89
votes
23
replies
17k
views
16 follow
Forum: How Did You Learn Good Practice In Bioinformatics/Computational Biology?
bioinformatics Forum
written 7.2 years ago by ▴ 490
88
votes
32
replies
6.7k
views
11 follow
Forum: Vintage / unconventional pictures for Bioinformatics
picture history Forum
3.1 years ago by Pierre Lindenbaum 135k
88
votes
24
replies
4.1k
views
10 follow
Forum: How To Be Helpful As A Biostar Moderator/Editor?
biostar Forum
8.1 years ago by aidan-budd 1.9k
86
votes
29
replies
7.0k
views
9 follow
Forum: What is the future of EMBL-EBI (and European bioinformatics in general) in case of a 'Brexit'?
EBI EU politics Forum brexit
4.8 years ago by Michael Dondrup 48k
84
votes
26
replies
2.1k
views
12 follow
Forum: is fortran still used in bioinformatics?
R FORTAN Forum
updated 4 months ago by 48k • written 22 months ago by ▴ 180
83
votes
30
replies
4.1k
views
18 follow
Forum: Optimal design for bioinformatics "lab" space?
lab space design Forum
updated 6.0 years ago by ★ 2.3k • written 6.0 years ago by 18k
82
votes
32
replies
5.0k
views
13 follow
Forum: Shall We Go Back To Stackexchange?
Forum
updated 8.4 years ago by ★ 4.0k • written 8.4 years ago by ★ 3.5k
81
votes
21
replies
3.3k
views
16 follow
Forum: [UPDATE] Thrown into a Bioinformatics Position
beginner new scared help Forum
updated 4.8 years ago by 16k • written 4.8 years ago by ★ 3.1k
81
votes
34
replies
13k
views
11 follow
Forum: Benchmark - Comparison of Different NGS Mappers
mRNA-Seq benchmark NGS mapper News Forum
6.3 years ago by David Langenberger 9.6k
80
votes
34
replies
3.0k
views
11 follow
Forum: Authorship issues- how to resolve?
authorship Forum
updated 5.9 years ago by ▴ 50 • written 5.9 years ago by ▴ 460
80
votes
23
replies
3.5k
views
11 follow
Forum: Are we facing a bioinformatician shortage?
jobs training salary Forum
updated 6.0 years ago by ▴ 190 • written 6.4 years ago by ★ 3.0k
78
votes
34
replies
6.2k
views
15 follow
Forum: Do bioinformaticians often break molecular biologists' hearts by being the first author?
career authorship Forum
updated 5.4 years ago by ★ 4.3k • written 5.4 years ago by ▴ 640
78
votes
31
replies
4.8k
views
14 follow
Forum: Do Bad Questions Even Exist?
general biostar education Forum
updated 7.4 years ago by 48k • written 10.1 years ago by 10k
72
votes
14
replies
1.5k
views
12 follow
Forum: Things you wish you knew when entering the bioinformatics field
pitfalls advice forum Forum
updated 6 months ago by 6.9k • written 6 months ago by 9.2k
72
votes
18
replies
5.7k
views
17 follow
Forum: What Is One Book Every Bioinformatician Should Read?
books Forum
updated 5.7 years ago by ▴ 200 • written 7.8 years ago by 6.9k
71
votes
28
replies
4.3k
views
10 follow
Forum: Bioinformatics commercial services as a risk factor for scientific misconduct
ethics misconduct commercial services Forum
updated 6.2 years ago by 32k • written 6.2 years ago by ▴ 90
70
votes
19
replies
3.5k
views
13 follow
Forum: Thrown Into A Bioinformatics Position
analysis Forum computational bioinformatics Latest
updated 4 days ago by 32k • written 5.5 years ago by ▴ 170
69
votes
39
replies
2.4k
views
14 follow
Forum: Preparation for Bioinformatics.SE closing
biostars Forum
updated 3.9 years ago by ▴ 740 • written 3.9 years ago by 13k
67
votes
40
replies
6.7k
views
12 follow
Forum: I want to re-open the old debate: python or perl ?
perl python Forum
updated 6.4 years ago by 17k • written 6.4 years ago by ★ 2.8k
66
votes
48
replies
5.6k
views
16 follow
Forum: Stranger Things: unexpected limitations of popular tools
samtools hisat2 errors Forum
updated 3.5 years ago by ★ 2.7k • written 3.8 years ago by 87k
66
votes
21
replies
4.1k
views
18 follow
Forum: Is there anyone who was a pure biologist?
next-gen Forum
updated 20 months ago by 32k • written 7.0 years ago by ▴ 100
1,338 results • Page 1 of 27
Recent Votes
Comment: A simple tutorial for a complex ComplexHeatmap
Answer: Hunting invisible characters?
BWA error: no ID within the read group line
Answer: differential expression analysis
Answer: How to verify putative miRNA
Comment: How to make consensus sequence from aligned reads ?
Answer: GSEA for RNA_seq dataset
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Recent Replies
Comment: How can I Extract Transposable element from a new genome assembly? by GenoMax 99k
Align your assemblies to each other using an aligner like `lastz` ([**LINK**][1]). You could also use `blat` if you are sure the assemblies…
Comment: Trimmomatic-0.36 not finding adapter directory by GenoMax 99k
It is going to take as long as it takes. You need to be patient and wait until the prompt returns. Based on speed of your CPU/disk things c…
Comment: Hunting invisible characters? by GenoMax 99k
You could have looked at the file using `cat -vet` which would have shown all characters in the file. Printable and non.
Comment: Can't count features with featurecount by avelarbio46 ▴ 10
I'm not sure about three things: What does -M and -O do exactly that could benefit in my case? Why should I specify -t if I don't necessa…
Comment: Hunting invisible characters? by geneticatt ▴ 120
Thank you, using `dos2unix` worked perfectly!
Answer: Hunting invisible characters? by Mensur Dlakic ★ 10k
You may want to read [**this**][1]. I think you may be able to fix your adaper file by typing: dos2unix i5R.txt If an error pops …
Comment: RNA seq analysis; two samples by Ram 32k
Please do not delete posts that have comments/answers.
Comment: ICE normalization using JuicerTools by sckinta ▴ 610
Is there a way to extract ICE bias from balanced .cool file? I know that you can find weight associated with each bin by `cooler dump --bal…
Comment: How to verify putative miRNA by boczniak767 ▴ 740
Yeah, miRNA could be only verified in wet-lab. Take a look at [stem-loop PCR][1]. I've used it and it works really well. [1]: https://j…
Comment: R - A bioinformatic analysis programming language for NGS by Ram 32k
Because this is a plain copy-paste from the novogene page, it has numbers (pointers to specific references that the source has), but not th…
Comment: X amino acid in ensembl by Mensur Dlakic ★ 10k
This is correct. `X` means any amino acid. Most substitution matrices apply identical penalty (`-1`) when any amino-acid is aligned with X …
Answer: differential expression analysis by Mensur Dlakic ★ 10k
I think the numbers you cite are the most extreme values that people will still accept. In most cases I would go for lower p-values (at lea…
Comment: Trimmomatic-0.36 not finding adapter directory by willbrown • 0
The two reads are roughly 23GB each, the only other large output file generated in my last attempt was the trimlog file, which was only 1.5…
Comment: A simple tutorial for a complex ComplexHeatmap by ATpoint 48k
You always normalize data before z-scoring it. The Z-score just calculate like the "deviation" of every sample from the mean of all samples…
Answer: GSEA for RNA_seq dataset by ATpoint 48k
I tried to explain my understanding of (f)GSEA and why one should use all (expressed) genes here over at this Bioc post: https://support…
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