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412 results • Page
3 of 9
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2
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1
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61k
views
Tutorial:
Docker denied: requested access to the resource is denied
docker
updated 22 months ago by
LauferVA
4.2k • written 22 months ago by
kodwings
▴ 20
83
votes
34
replies
19k
views
22 follow
Tutorial:
Enrichment Analysis, Clustering and Scoring with pathfindR
enrichment
R
pathway
active-subnetwork
updated 11 months ago by
adelheidkratzer
• 0 • written 5.9 years ago by
egeulgen
★ 1.3k
87
votes
2
replies
59k
views
6 follow
Tutorial:
How To Ask Good Questions On Technical And Scientific Forums
how-to
updated 14 months ago by
Ram
43k • written 10.9 years ago by
Eric Normandeau
11k
16
votes
5
replies
13k
views
6 follow
Tutorial:
Easy way to do GO enrichment in R
enrichr
RNA-Seq
gene
R
updated 14 months ago by
Ram
43k • written 6.5 years ago by
ahmad mousavi
▴ 800
3
votes
2
replies
2.9k
views
Tutorial:
Running fastq_screen on your data
fastqscreen
2.2 years ago by
rohitsatyam102
▴ 860
9
votes
14
replies
3.8k
views
6 follow
Tutorial:
Install required dependencies for GATK4 on remote server without root privilege
gatk
updated 18 months ago by
Angelina_G
▴ 10 • written 2.2 years ago by
thehung92phuyen
▴ 40
76
votes
14
replies
16k
views
15 follow
Tutorial:
List Of Bioinformatics Books
books
updated 15 months ago by
Ram
43k • written 11.0 years ago by
Medhat
9.7k
35
votes
13
replies
14k
views
9 follow
Tutorial:
How to get FASTQ reads from the Short Read Archive (SRA)
fastq
sra
updated 2.3 years ago by
liorglic
★ 1.4k • written 2.6 years ago by
Istvan Albert
100k
7
votes
13
replies
13k
views
Tutorial:
Merging multiple vcf files into Single vcf file
vcf
bcftools
updated 11 months ago by
Ram
43k • written 5.2 years ago by
jaybee
▴ 170
16
votes
9
replies
7.1k
views
Tutorial:
TCGA transcriptome data to R (DESeq2)
TCGA
GDC
DESeq2
updated 23 months ago by
dk0319
▴ 70 • written 2.4 years ago by
Barry Digby
★ 1.3k
0
votes
0
replies
11k
views
Tutorial:
Using PatMatch to search for nucleic and protein sequence patterns and integrate the results with Python/Jupyter for downstream analysis
Jupyter
Python
pattern
2.3 years ago by
Wayne
★ 2.0k
12
votes
4
replies
5.4k
views
Tutorial:
*FREE course* Bioinformatics for Biologists: An Introduction to Linux, Bash Scripting, and R
linux
R
updated 15 months ago by
Ram
43k • written 3.3 years ago by
garden_giessen
▴ 130
30
votes
10
replies
82k
views
9 follow
Tutorial:
Installing an updated R version (>=4.0) using conda
software-error
R
updated 14 months ago by
Ram
43k • written 3.2 years ago by
Nitin Narwade
★ 1.6k
46
votes
6
replies
25k
views
Tutorial:
Greatly speed up conda by using mamba
software
updated 2.3 years ago by
cameron.cowan
▴ 50 • written 3.1 years ago by
rpolicastro
13k
150
votes
70
replies
51k
views
20 follow
Tutorial:
Network plot from expression data in R using igraph
microarray
RNA-Seq
R
network
updated 8 months ago by
Raheleh
▴ 260 • written 6.5 years ago by
Kevin Blighe
88k
15
votes
2
replies
2.1k
views
Tutorial:
Germline variant calling pipeline using Snakemake
snakemake
GATK
germline_variant_calling
updated 19 months ago by
brunobsouzaa
▴ 830 • written 2.6 years ago by
nhaus
▴ 360
196
votes
44
replies
72k
views
33 follow
Tutorial:
Analysing Microarray Data In Bioconductor
microarray-analysis
bioconductor
updated 16 months ago by
Ram
43k • written 11.6 years ago by
Obi Griffith
20k
6
votes
2
replies
1.6k
views
Tutorial:
GSE28955 - Agilent-016436 Human miRNA Microarray 1.0
limma
GPL6955
Microarray
miRNA
Agilent
2.7 years ago by
Kevin Blighe
88k
0
votes
0
replies
1.2k
views
Tutorial:
Alignment-free RNA-seq Differential Gene Expression Analysis with Kallisto & Sleuth
dge
kallisto
rnaseq
sleuth
2.8 years ago by
mo
• 0
3
votes
1
reply
3.4k
views
Tutorial:
Affymetrix HTA 2.0 id conversion
biomart
HTA
affymetrix
Affymetrix
2.0
updated 3 months ago by
MaxF
▴ 120 • written 2.8 years ago by
Kevin Blighe
88k
45
votes
2
replies
37k
views
Tutorial:
Mapping Reads With Bwa And Bowtie
bowtie
bwa
updated 15 months ago by
Ram
43k • written 12.1 years ago by
MSU course 2011
▴ 540
100
votes
106
replies
47k
views
26 follow
Tutorial:
Produce PCA bi-plot for 1000 Genomes Phase III - Version 2
1000genomes
PLINK
PCA
updated 5 weeks ago by
psb7
▴ 20 • written 5.7 years ago by
Kevin Blighe
88k
0
votes
0
replies
1.1k
views
Tutorial:
Event & tutorial: open software tools and AI for improving bioimaging (SLURM, containerisation)
containerisation
bioimaging
SLURM
health
2.9 years ago by
Erin Haskell | ELIXIR
▴ 470
1
vote
0
replies
1.3k
views
Tutorial:
Slurm and parallelization
slurm
2.9 years ago by
Juke34
8.6k
1
vote
0
replies
1.3k
views
Tutorial:
How to: make Camellia sinensis var. sinensis (black tea) custom annotation files for BINGO Cytoscape
bingo
cytoscape
2.9 years ago by
Pratik
★ 1.0k
14
votes
1
reply
3.1k
views
Tutorial:
PCR Primer or Primer+Probe design
pcr
probe
rt-pcr
qpcr
primer
updated 2.9 years ago by
jared.andrews07
★ 16k • written 2.9 years ago by
Kevin Blighe
88k
117
votes
66
replies
46k
views
20 follow
Tutorial:
[Deprecated] Fast download of FASTQ files from the European Nucleotide Archive (ENA)
aspera
fastq-dump
Fastq
ENA
updated 14 months ago by
Ram
43k • written 5.9 years ago by
ATpoint
82k
4
votes
0
replies
1.2k
views
Tutorial:
Long Read Genome Assembly Video Series
long-read
genome-assembly
2.9 years ago by
pbpanigrahi
▴ 420
0
votes
1
reply
1.1k
views
Tutorial:
Add Customized Filters in GDC
gdc
updated 3.0 years ago by
cpad0112
21k • written 3.0 years ago by
Zhenyu Zhang
★ 1.2k
12
votes
20
replies
14k
views
10 follow
Tutorial:
Associating VDJ clonotyping data with scRNA-seq in Seurat
single-cell
RNA-Seq
seurat
TCR
updated 14 months ago by
Ram
43k • written 4.9 years ago by
jared.andrews07
★ 16k
4
votes
2
replies
2.5k
views
Tutorial:
Apply beagle5.0 to make imputation and phasing under CentOS and PBS
beagle5.0
beagle
updated 14 months ago by
Ram
43k • written 4.9 years ago by
Shicheng Guo
★ 9.4k
55
votes
14
replies
93k
views
10 follow
Tutorial:
Using R in Conda
R
updated 14 months ago by
Ram
43k • written 3.8 years ago by
rohitsatyam102
▴ 860
99
votes
16
replies
34k
views
18 follow
Tutorial:
Heatmaps in R
heatmap
R
updated 14 months ago by
Ram
43k • written 7.8 years ago by
Jean-Karim Heriche
27k
23
votes
2
replies
5.9k
views
Tutorial:
Extracting genomic feature sequences from GTF/GFF files with AGAT
extract
GTF
GFF
sequence
updated 20 months ago by
eggrandio
▴ 40 • written 3.1 years ago by
Juke34
8.6k
32
votes
6
replies
12k
views
Tutorial:
A simple tutorial for a complex ComplexHeatmap
clustering
pam
arthritis
ComplexHeatmap
updated 14 months ago by
Ram
43k • written 4.0 years ago by
Kevin Blighe
88k
8
votes
0
replies
2.8k
views
Tutorial:
Pipes in R: An introduction and some advanced tips and tricks.
R
3.1 years ago by
rpolicastro
13k
239
votes
176
replies
96k
views
73 follow
Tutorial:
Survival analysis of TCGA patients integrating gene expression (RNASeq) data
RNA-Seq
Survival
TCGA
updated 11 months ago by
Ram
43k • written 8.8 years ago by
TriS
★ 4.7k
6
votes
0
replies
2.4k
views
Tutorial:
data.frames in R: How vectorization can save you time and heartache
R
3.1 years ago by
rpolicastro
13k
8
votes
1
reply
1.4k
views
Tutorial:
Split a 'linearised' (flattened) FASTA sequence into multi-line using AWK
fasta
awk
updated 14 months ago by
Ram
43k • written 3.2 years ago by
Kevin Blighe
88k
22
votes
10
replies
7.1k
views
Tutorial:
Finding the significance of the overlap between 2 or more gene sets using simulation in R.
tidyverse
gene
R
updated 14 months ago by
Ram
43k • written 3.7 years ago by
rpolicastro
13k
12
votes
2
replies
1.3k
views
Tutorial:
Factors in R - How do they work, and how did I break my data?
R
updated 14 months ago by
Ram
43k • written 3.2 years ago by
rpolicastro
13k
149
votes
35
replies
62k
views
33 follow
Tutorial:
Tools For Metagenomic Data Analysis
metagenomics
next-gen
updated 14 months ago by
Ram
43k • written 11.5 years ago by
vijay
★ 1.6k
16
votes
7
replies
16k
views
Tutorial:
Integrating VDJ sequencing data with Seurat
VDJ
Seurat
sc-RNAseq
clonotyping
updated 14 months ago by
Ram
43k • written 4.9 years ago by
atakanekiz
▴ 310
298
votes
33
replies
109k
views
25 follow
Tutorial:
Cheat Sheet For One-Based Vs Zero-Based Coordinate Systems
coordinates
updated 14 months ago by
Ram
43k • written 10.6 years ago by
Obi Griffith
20k
6
votes
2
replies
5.0k
views
Tutorial:
Yes .. BBMap can do that! - Part III clumpify (mark (and dedupe) duplicates without alignment), mutate (create mutant genomes) and other miscellaneou…
bbmap
5 months ago by
GenoMax
142k
11
votes
0
replies
6.9k
views
Tutorial:
Yes .. BBMap can do that! - Part II randomreads (read simulation), demuxbyname/filterbyname, bbsplit (read binning/decontamination) and pileup (cover…
bbmap
updated 14 months ago by
Ram
43k • written 3.3 years ago by
GenoMax
142k
10
votes
0
replies
5.0k
views
Tutorial:
Yes .. BBMap can do that! - Part I : bbmap (aligner), bbduk (scan/trim), repair (fix PE reads) and reformat (format conversions)
bbmap
updated 14 months ago by
Ram
43k • written 3.3 years ago by
GenoMax
142k
11
votes
1
reply
2.7k
views
Tutorial:
Guidelines for the computational analysis of single-cell RNA sequencing data
scRNA-seq
updated 14 months ago by
Ram
43k • written 3.4 years ago by
Mensur Dlakic
★ 27k
40
votes
9
replies
11k
views
10 follow
Tutorial:
Resources for flow cytometry bioinformatics
single-cell
flow-cytometry
updated 14 months ago by
Ram
43k • written 10.1 years ago by
Ahill
★ 1.9k
0
votes
1
reply
3.0k
views
Tutorial:
Getting the lineage of an organism from NCBI taxonomy data
biosql
python
taxonomy
updated 14 months ago by
Ram
43k • written 5.8 years ago by
vimalkvn
▴ 320
412 results • Page
3 of 9
Recent Votes
Answer: RSeQC : infer_experiment.py Error: Could not retrieve index file
A: Best de novo assembler for insect genome ?
Comment: sci-RNA-seq
The Biostar Herald for Monday, May 13, 2024
Answer: Finding variants within a subset of a BAM file
Answer: Extracting only 4-fold degenerate sites from gene sequences/alignments?
Answer: Extracting only 4-fold degenerate sites from gene sequences/alignments?
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Recent Replies
Comment: featureCounts output summary assigned value higher than uniquely mapped reads fr
by
Ram
43k
Please accept your own answer to mark the post as solved.
Comment: RSeQC : infer_experiment.py Error: Could not retrieve index file
by
Ram
43k
A small educational note: if an answer was helpful, you should upvote it; if the answer resolved your question, you should mark it as accep…
Comment: RSeQC : infer_experiment.py Error: Could not retrieve index file
by
Prawesh
• 0
thanks, it's working now.
Comment: Genome Visualization Tools
by
GenoMax
142k
@cmdcolin has a great list here: https://github.com/cmdcolin/awesome-genome-visualization
Answer: RSeQC : infer_experiment.py Error: Could not retrieve index file
by
Trivas
★ 1.8k
You need to have indexed your bam/sam file using `samtools index`
Answer: Using ggplotly in R
by
jared.andrews07
★ 16k
The simple answer is to plot it natively with plotly rather than use `ggplotly`. See [this stackoverflow answer](https://stackoverflow.com/…
Comment: Why does assigning genes with biomart give me different values than using a tran
by
GenoMax
142k
According to HUGO there is only one [**ZSCAN2 gene**][1] and it points to the first Ensembl gene ID (ENSG00000176371). Ensembl is annotatin…
Answer: RNAseq RNA content
by
noodle
▴ 580
There are many tools for this. I recommend [gatk (picard) CollectRnaSeqMetrics][1] or alternatively [RSeQC][2] and then run [multiqc][3…
Comment: Where to get the following bed file?
by
wyuan37
• 0
Hi, I emailed them and did get the bed file! Thank you so much.
Comment: Where to get the following bed file?
by
wyuan37
• 0
Thanks! Super helpful.
Answer: Post-imputation QC for input into GWAS analyses
by
LauferVA
4.2k
1. Imputation algorithms will output log files indicating, among other things, accuracy of imputation. We can't comment on specifics as we …
Comment: Using Cutadapt to trim adapters from paired-end small RNA sequence data
by
GenoMax
142k
You can simply ignore read 2. It is not adding any information since your small RNA's are going to be small and were completely sequenced b…
Comment: Overlapping Ranges within Granges object
by
ntsopoul
▴ 60
does this work with a single .bed file?
Comment: Finding variants within a subset of a BAM file
by
ramiro.barrantes
• 0
Actually, I found a solution in downloading "bam slices" from TCGA ([https://docs.gdc.cancer.gov/API/Users_Guide/BAM_Slicing/][1]) , which …
Comment: Overlapping Ranges within Granges object
by
ntsopoul
▴ 60
![enter image description here][1]Here is some representative data and an image in igv of one gene. I have loaded the .bed file in igv. As …
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