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384 results • Page
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Sort: Views
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Views
Votes
Replies
3
votes
3
replies
8.4k
views
Tutorial:
High Speed Downloading of SRA, SAM and Fastq Files
fastq
sam
Aspera-Connect
sra
updated 6 months ago by
Ram
41k • written 6.0 years ago by
Wenhu_Cao
▴ 100
20
votes
4
replies
8.4k
views
Tutorial:
DE analysis by edgeR
RNA-Seq
R
edgeR
updated 8 months ago by
Ram
41k • written 7.2 years ago by
fi1d18
★ 4.2k
81
votes
8
replies
8.3k
views
6 follow
Tutorial:
Current Topics in Genome Analysis 2014 (collection of educational lectures)
workshop
lectures
genomics
training
updated 9 months ago by
Ram
41k • written 9.5 years ago by
Malachi Griffith
19k
14
votes
0
replies
8.2k
views
Tutorial:
Video: ChIP-Seq Analysis and Visualization using Galaxy and IGB.
IGB
ChIP-Seq
Galaxy
MACS
RNA-Seq
updated 20 months ago by
Ram
41k • written 8.9 years ago by
Nowlan Freese
▴ 860
8
votes
7
replies
8.1k
views
Tutorial:
Getting Cegma/Genewise To Work On Centos, Rhel
centos
genewise
rhel
cegma
updated 22 months ago by
Ram
41k • written 10.0 years ago by
Adrian Pelin
★ 2.6k
12
votes
5
replies
8.1k
views
Tutorial:
How To Install BioPerl Without Root Privileges in Ubuntu/Linxu
bioperl
updated 8 months ago by
Ram
41k • written 7.5 years ago by
Shicheng Guo
★ 9.3k
19
votes
13
replies
8.0k
views
10 follow
Tutorial:
Setting up Aspera Connect (ascp) on Linux and macOS
ascp
aspera
updated 5 weeks ago by
jude
• 0 • written 17 months ago by
ATpoint
78k
0
votes
0
replies
7.9k
views
Tutorial:
Multicollinearity VIF (Variance Inflation Factor) Analysis in Panel data EVIEWS 9
R
eviews
updated 6 months ago by
Ram
41k • written 5.9 years ago by
helgasaraswati
▴ 10
50
votes
1
reply
7.9k
views
Tutorial:
Basic normalization, batch correction and visualization of RNA-seq data
edger
RNA-seq
updated 10 months ago by
Ram
41k • written 3.2 years ago by
ATpoint
78k
16
votes
11
replies
7.9k
views
Tutorial:
How to Use Biostars, Part II: Post types, Deleting, (Un)Subscribing, Linking and Bookmarking
meta
how-to
documentation
8 months ago by
Ram
41k
20
votes
2
replies
7.8k
views
Tutorial:
Creating a karyotype plot with genes using karyoploteR
karyoploteR
visualization
R
genome
updated 6 months ago by
Ram
41k • written 6.0 years ago by
bernatgel
★ 3.4k
6
votes
1
reply
7.7k
views
Tutorial:
Expression Analysis Of Rna-Seq Experiments With Tophat And Cufflinks
tophat
RNA-seq
cufflinks
updated 9 months ago by
Ram
41k • written 11.6 years ago by
Istvan Albert
99k
12
votes
0
replies
7.6k
views
Tutorial:
Plot average methylation levels across TSS region
bedtools
RRBS
R
methylation
updated 20 months ago by
Ram
41k • written 8.9 years ago by
TriS
★ 4.7k
42
votes
19
replies
7.6k
views
8 follow
Tutorial:
Designing Command-Line Interfaces (Clis) For Scientific Software
software
updated 9 months ago by
Ram
41k • written 10.8 years ago by
Daniel Standage
4.1k
26
votes
17
replies
7.4k
views
8 follow
Tutorial:
virtualenv - running Python based software in isolation
python
virtualenv
updated 15 months ago by
Ram
41k • written 9.3 years ago by
Ian
6.0k
32
votes
4
replies
7.3k
views
Tutorial:
Fast LD computation from VCF files using tomahawk
ld
snp
vcf
vcftools
bcftools
updated 8 months ago by
Ram
41k • written 5.1 years ago by
firestar
★ 1.6k
6
votes
4
replies
7.3k
views
Tutorial:
Overlay Multiple Tracks In Ucsc Browser [Quick Minimal Tutorial]
visualization
ChIP-seq
RNA-seq
ucsc-genome-browser
bigwig
updated 9 months ago by
Ram
41k • written 10.7 years ago by
Sukhi Singh
11k
60
votes
2
replies
7.2k
views
Tutorial:
Table Of Contents To All Review Paper Compilations On Biostar
papers
updated 9 months ago by
Ram
41k • written 11.3 years ago by
Istvan Albert
99k
24
votes
4
replies
7.2k
views
Tutorial:
Using EnrichedHeatmap for visualization of NGS experiments
heatmap
chip-seq
atac-seq
EnrichedHeatmap
updated 8 months ago by
Ram
41k • written 4.3 years ago by
ATpoint
78k
12
votes
11
replies
7.1k
views
Tutorial:
How to upload your RNA-Seq data to NCBI Sequence Read Archive (SRA)
NCBI
Bioproject
SRA
BioSample
updated 7 months ago by
Ram
41k • written 6.7 years ago by
CandiceChuDVM
★ 2.4k
4
votes
2
replies
7.1k
views
Tutorial:
Analyzing Genomewide Association Study Data: A Tutorial Using Plink
plink
gwas
updated 9 months ago by
Ram
41k • written 11.6 years ago by
Istvan Albert
99k
64
votes
5
replies
7.1k
views
10 follow
Tutorial:
Informatics of RNA-Seq.
next-gen
RNA-Seq
updated 13 months ago by
Ram
41k • written 8.3 years ago by
GouthamAtla
12k
15
votes
1
reply
6.8k
views
Tutorial:
A compilation of conversion tools for BED, SAM/BAM, psl, pslx, blast tabular and blast xml
psl
SAM
blast
BED
updated 6 months ago by
Ram
41k • written 6.1 years ago by
Joseph Hughes
★ 3.0k
15
votes
2
replies
6.7k
views
Tutorial:
Genotype And Snp Calling Review Papers
snp
updated 9 months ago by
Ram
41k • written 11.3 years ago by
Istvan Albert
99k
2
votes
0
replies
6.7k
views
Tutorial:
How to - Install Python packages in a virtual environment (virtualenv)
ubuntu
cutadapt
virtualenv
python
windows
updated 6 months ago by
Ram
41k • written 6.0 years ago by
vimalkvn
▴ 320
5
votes
5
replies
6.7k
views
Tutorial:
use firehose_get to download TCGA data
tcga
RNA-Seq
updated 6 months ago by
Ram
41k • written 6.1 years ago by
jmzeng1314
▴ 130
21
votes
5
replies
6.6k
views
Tutorial:
Analysis of single-cell RNA-seq data
single-cell
RNA-Seq
updated 8 months ago by
Ram
41k • written 7.4 years ago by
Geparada
★ 1.5k
16
votes
0
replies
6.5k
views
Tutorial:
Introduction To Ngs Bioinformatics By The Bioinformatics Team At The University Of Texas
course
training
updated 9 months ago by
Ram
41k • written 11.5 years ago by
Istvan Albert
99k
8
votes
12
replies
6.5k
views
Tutorial:
Create your own VPN to access work resources from home
tinyproxy
OpenVPN
VPN
updated 8 months ago by
Ram
41k • written 7.5 years ago by
John
13k
8
votes
2
replies
6.5k
views
Tutorial:
Troubleshooting issues with SURPI pipeline for pathogen identification from complex metagenomic NGS data.
metagenomics
clinical-genomics
updated 22 months ago by
Ram
41k • written 9.1 years ago by
GouthamAtla
12k
23
votes
19
replies
6.4k
views
10 follow
Tutorial:
MutScan: Detect important mutations by scanning FastQ files directly
fastq
mutation
cancer
target
updated 8 months ago by
Ram
41k • written 7.2 years ago by
chen
★ 2.5k
21
votes
9
replies
6.4k
views
8 follow
Tutorial:
Interactive Perl Learning
perl
updated 9 months ago by
Ram
41k • written 10.5 years ago by
Medhat
9.7k
14
votes
2
replies
6.3k
views
Tutorial:
ChIP-seq analysis tutorial
ChIP-Seq
updated 8 months ago by
Ram
41k • written 8.3 years ago by
Ming Tommy Tang
★ 3.6k
21
votes
10
replies
6.3k
views
Tutorial:
Finding the significance of the overlap between 2 or more gene sets using simulation in R.
tidyverse
gene
R
updated 8 months ago by
Ram
41k • written 3.3 years ago by
rpolicastro
12k
16
votes
9
replies
6.3k
views
Tutorial:
TCGA transcriptome data to R (DESeq2)
TCGA
GDC
DESeq2
updated 18 months ago by
dk0319
▴ 70 • written 2.0 years ago by
Barry Digby
★ 1.3k
6
votes
0
replies
6.2k
views
Tutorial:
Leveraging Flow Order And Flow Signals From Ion Torrent Data
ion-torrent
updated 9 months ago by
Ram
41k • written 11.6 years ago by
Biostar User
▴ 360
16
votes
6
replies
6.2k
views
Tutorial:
How to make your snakefiles self-documenting
snakemake
updated 8 months ago by
Ram
41k • written 7.1 years ago by
Botond Sipos
★ 1.7k
29
votes
5
replies
6.2k
views
Tutorial:
Gnu Parallel Tutorial
parallel
gnu-parallel
updated 9 months ago by
Ram
41k • written 10.2 years ago by
ole.tange
★ 4.4k
11
votes
0
replies
6.1k
views
Tutorial:
Yes .. BBMap can do that! - Part II randomreads (read simulation), demuxbyname/filterbyname, bbsplit (read binning/decontamination) and pileup (cover…
bbmap
updated 8 months ago by
Ram
41k • written 2.9 years ago by
GenoMax
136k
20
votes
7
replies
6.1k
views
Tutorial:
Showing up and down regulated GO and pathway using enrichR and ggplot2
ggplot2
RNA-Seq
R
updated 8 months ago by
Ram
41k • written 5.2 years ago by
ahmad mousavi
▴ 770
14
votes
7
replies
6.1k
views
Tutorial:
Visualization of ChIP-Seq peak overlaps using HOMER mergePeaks and UpSetR
ChIP-Seq
peaks
R
HOMER
updated 8 months ago by
Ram
41k • written 7.6 years ago by
steve
★ 3.4k
14
votes
31
replies
6.1k
views
6 follow
Tutorial:
gene set filter/selection for training ab initio annotation tools
geneset
genome
gene
abinitio
updated 9 months ago by
Ram
41k • written 4.3 years ago by
Juke34
8.4k
19
votes
7
replies
6.1k
views
Tutorial:
scRNA-seq CITE-seq-count bioinformatics
CITE-seq-count
scRNA-seq
RNA-Seq
updated 4 months ago by
ATpoint
78k • written 3.7 years ago by
colindaven
5.8k
34
votes
15
replies
6.0k
views
6 follow
Tutorial:
How to do data cleaning for VCF genetic file
vcf
updated 8 months ago by
Ram
41k • written 4.5 years ago by
Shicheng Guo
★ 9.3k
4
votes
1
reply
6.0k
views
Tutorial:
Chip-Seq Data Analysis Tutorial
chip-seq
updated 9 months ago by
Ram
41k • written 11.6 years ago by
Biostar User
▴ 360
36
votes
5
replies
5.9k
views
Tutorial:
[Beginner] Introduction to bioinformatics file types
fastq
fasta
bed
SAM
updated 3 months ago by
gallardodiazmiriam
▴ 20 • written 8.4 years ago by
Luke Crichton
▴ 410
22
votes
2
replies
5.8k
views
Tutorial:
Use a workflow management tool to manage your computational pipelines
workflow-management
snakemake
pipeline
updated 8 months ago by
Ram
41k • written 7.1 years ago by
Eric Lim
★ 2.1k
18
votes
2
replies
5.8k
views
Tutorial:
Update kraken databases
database
kraken
k-mer
python
updated 6 months ago by
Ram
41k • written 6.6 years ago by
Sej Modha
5.3k
13
votes
6
replies
5.7k
views
Tutorial:
Trim & align paired-end reads in a single pass
align
trim
fastq
pipe
paired-end
updated 8 months ago by
Ram
41k • written 7.5 years ago by
dariober
14k
13
votes
7
replies
5.6k
views
Tutorial:
STARsolo config for 10x Chromium v1, v2, v3
Chromium
10x
STARsolo
STAR
updated 8 months ago by
Ram
41k • written 3.2 years ago by
Kevin Blighe
86k
384 results • Page
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Recent Votes
Comment: gatk SelectVariants is giving dupilicate allele error while extracting SNPs out
Answer: How to query 1000 genomes project VCF files for specific regions without downloa
Answer: Issue softclipping reads when they belong and don't belong to a common amplicon
Answer: Issue softclipping reads when they belong and don't belong to a common amplicon
Answer: how to remove multiple columns from a file in R
Answer: how to remove multiple columns from a file in R
Answer: Read count vs Depth
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Comment: Normalization of RNA captureSeq data (<20 genes captured)
by
ATpoint
78k
What is the question exactly? With only 20 genes you basically **must** use the spike-ins as that few genes do not really allow to normaliz…
Comment: Building reference dbSNP file using WGS samples
by
analyst
▴ 10
Dear [Brian][1] I realized that problem is with callvariants.sh script because when I called variants using freebayes I did not find aforem…
Comment: gatk SelectVariants is giving dupilicate allele error while extracting SNPs out
by
analyst
▴ 10
Its bbmap tool callvariants.sh. I think you are right because I did not get such error when I called variants through freebayes.
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j.gleixner
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Since all your sequences have the same length (say M) and you are only looking concerned with hamming distance you can encode your sequence…
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using wikidata: https://query.wikidata.org/sparql?query=%0ASELECT%20%3Fitem%20%3FitemLabel%20%3FitemDescription%20%3Fprevalence%20%3Fdoid%0…
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ju_ra
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Thank you very much! I see you decided to go for Bowtie as aligner. Do you see any issue in using STAR (as a splice aware reader technicall…
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Elisabeth Gasteiger
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Please contact the UniProt helpdesk whenever you find such annotations, especially in these cases where the GO evidence/source tag says "Un…
Comment: gatk SelectVariants is giving dupilicate allele error while extracting SNPs out
by
Pierre Lindenbaum
158k
again, it's a problem with the variant caller, which is ?
Comment: gatk SelectVariants is giving dupilicate allele error while extracting SNPs out
by
analyst
▴ 10
Not only opposite case but same case nucleotides are also present in vcf fle. ![enter image description here][1] [1]: /media/images/4…
Comment: gatk SelectVariants is giving dupilicate allele error while extracting SNPs out
by
analyst
▴ 10
Yes [Pierre][1] there is lower case **g** in **REF** and upper case **G** in **ALT** field. My question is that why variant caller is calli…
Answer: gatk SelectVariants is giving error while extracting SNPs out of vcf file
by
Pierre Lindenbaum
158k
This is an error in your input vcf file. The REF allele MUST be différent from the ALT allele.
Comment: gatk SelectVariants is giving error while extracting SNPs out of vcf file
by
analyst
▴ 10
I observed that my reference genomic fasta file contains both lower case and upper case nucleotides. Do I need to convert lower case nucle…
Comment: Building reference dbSNP file using WGS samples
by
analyst
▴ 10
I observed that both lower and upper cases are present in reference fasta file. Is bbtool case sensitive that while calling variants it con…
Comment: Read count vs Depth
by
Mary
• 0
Thanks Brain Bushnell Referring to my query1: So u mean that for a particular gene, if total read count is 6, then depth may be just 1 or …
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