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460 results • Page 2 of 10
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21
votes
15
replies
2.1k
views
9 follow
Forum: Working abroad without a PhD
career
updated 10 months ago by 43k • written 4.6 years ago by ★ 1.4k
23
votes
15
replies
5.1k
views
6 follow
Forum: On the usage of Golang
assembly next-gen alignment
updated 11 months ago by 43k • written 5.5 years ago by ▴ 80
19
votes
15
replies
2.0k
views
6 follow
Forum: Cross out line in Biostars
meta
updated 11 months ago by 43k • written 5.9 years ago by 5.3k
57
votes
15
replies
6.2k
views
9 follow
Forum: Good practices while dealing with very large datasets
Big-Data Programming
updated 11 months ago by 43k • written 7.0 years ago by ★ 1.2k
28
votes
15
replies
2.2k
views
12 follow
Forum: Should bioinformaticians learn the scientific names of plants and animals?
sequence
updated 11 months ago by 43k • written 5.8 years ago by ▴ 10
22
votes
15
replies
8.8k
views
11 follow
Forum: RNASeq user experience with Novogene?
RNA-Seq
updated 11 months ago by 43k • written 5.8 years ago by ▴ 80
8
votes
15
replies
2.4k
views
7 follow
Forum: How to identify your analytic data is true?
RNA-Seq ChIP-Seq next-gen-sequencing
updated 11 months ago by 43k • written 6.7 years ago by ▴ 80
17
votes
14
replies
3.3k
views
Forum: Asking for feedback on a Python library for computing alignments
alignment Python
updated 10 months ago by ▴ 220 • written 2.3 years ago by ▴ 60
3
votes
14
replies
2.1k
views
6 follow
Forum: Search for gene variants
next-gen
updated 11 months ago by 43k • written 5.7 years ago by 8.3k
73
votes
14
replies
4.2k
views
12 follow
Forum: Things you wish you knew when entering the bioinformatics field
pitfalls advice
updated 10 months ago by 43k • written 3.6 years ago by ★ 16k
5
votes
14
replies
2.0k
views
Forum: immediate help needed for genome sequence analysis of bacteria
genome miseq webinar paired-end
updated 11 months ago by 43k • written 7.1 years ago by • 0
19
votes
14
replies
2.1k
views
Forum: What is the amount of sequencing data produced annually?
data research sequencing
updated 7 months ago by 22k • written 7 months ago by ▴ 70
9
votes
14
replies
2.4k
views
Forum: MS Bioinformatics Project
assembly sequencing alignment R SNP
updated 11 months ago by 43k • written 6.1 years ago by ▴ 10
12
votes
14
replies
2.4k
views
8 follow
Forum: Recovering bam files after unknown deletion in the storage
photorec bam sequencing
written 4.9 years ago by ▴ 70
17
votes
14
replies
1.9k
views
10 follow
Forum: What would be the trend in next few years in NGS era?
NGS sequencing
updated 11 months ago by 43k • written 5.5 years ago by ▴ 800
4
votes
13
replies
1.7k
views
Forum: Looking for Participants: Meenta NovaSeq RunShare Beta
RunShare next-gen NovaSeq
updated 10 months ago by 43k • written 4.8 years ago by ▴ 10
11
votes
13
replies
4.5k
views
6 follow
Forum: NGS Data storage solutions for small organisations or big labs
big-data HTS storage NGS metagenomics
updated 11 months ago by 43k • written 4.9 years ago by ▴ 830
30
votes
13
replies
3.7k
views
11 follow
Forum: what is your daily must see bioinformatics web site?
learn technology
updated 10 months ago by 43k • written 9.6 years ago by 9.7k
7
votes
13
replies
1.4k
views
Forum: How do you validate and verify your pipeline's software updates?
Validation Verification Quality-Assurance
updated 6 months ago by 43k • written 6 months ago by ▴ 10
1
vote
13
replies
2.8k
views
Forum: Bioinformatics computer upgrade help
computer
updated 10 months ago by 43k • written 3.8 years ago by ▴ 50
15
votes
13
replies
4.1k
views
8 follow
Forum: Does a PhD or work experience set you up better to do bioinformatics work in industry?
advice industry phd
updated 3 months ago by 3.9k • written 17 months ago by ▴ 30
12
votes
12
replies
1.4k
views
7 follow
Forum: Who qualify as a world class bioinformatician as seen in job ads
world-class bioinformatician qualification
updated 10 months ago by 43k • written 4.1 years ago by • 0
22
votes
12
replies
1.8k
views
6 follow
Forum: What were the most impactful Bioinformatics papers published this year
papers publications
updated 10 months ago by 43k • written 4.4 years ago by ▴ 520
23
votes
12
replies
1.9k
views
6 follow
Forum: Whole-Exome Analysis on a Mainstream Desktop PC
NGS whole-exome
updated 10 months ago by 43k • written 3.9 years ago by 11k
21
votes
12
replies
3.5k
views
Forum: How to Use Biostars Part-3: Formatting Text and Using GitHub Gists
meta how-to documentation
3 months ago by Ram 43k
4
votes
12
replies
1.5k
views
Forum: publicly available data
arrayexpress GEO SRA
updated 10 months ago by 43k • written 4.6 years ago by ▴ 110
26
votes
12
replies
7.5k
views
6 follow
Forum: Mapping God Found ‘Scientifically Dishonest’ By Anonymous Peer Reviewers
ngs
updated 11 months ago by ▴ 10 • written 10.5 years ago by ▴ 50
12
votes
12
replies
3.0k
views
Forum: Programming interview via Skype
programming-test interview
updated 11 months ago by 43k • written 5.9 years ago by ▴ 50
18
votes
12
replies
2.5k
views
6 follow
Forum: why GATK makes things more complicated ?
genomics
updated 11 months ago by 43k • written 5.2 years ago by ▴ 280
31
votes
12
replies
944
views
7 follow
Forum: Nextflow and self-made pipelines opinion
nextflow pipeline data-analysis
8 weeks ago by karlaarz ▴ 90
15
votes
11
replies
4.7k
views
9 follow
Forum: Methodological problems are extremely common for enrichment analysis - beware the pitfalls before you publish
GSEA DAVID enrichment-analysis -geneontology
updated 11 months ago by 43k • written 2.1 years ago by ★ 1.9k
21
votes
11
replies
2.0k
views
Forum: Most important International Big Data Projects: Genetics and Epigenetics
big-data
updated 11 months ago by 43k • written 5.7 years ago by ★ 9.4k
8
votes
11
replies
1.8k
views
Forum: What makes a good bionformatics prediction service from the point of user experience?
server prediction-service
updated 11 months ago by 43k • written 6.5 years ago by ▴ 40
14
votes
11
replies
6.7k
views
7 follow
Forum: Do you need a deduplication tool for FASTQ data in fastp?
deduplication fastp
updated 11 months ago by 43k • written 4.9 years ago by ★ 2.5k
17
votes
11
replies
980
views
Forum: How to overcome the imposter syndrome in bioinformatics?
advice
3 months ago by sil_bioinfo ▴ 40
23
votes
11
replies
2.0k
views
9 follow
Forum: file and directory management best practices
file-management
updated 10 months ago by 43k • written 3.7 years ago by ▴ 140
18
votes
11
replies
2.4k
views
8 follow
Forum: Create a File format to store Variant
hgvs json variant
updated 11 months ago by 43k • written 5.8 years ago by ★ 2.4k
13
votes
11
replies
4.7k
views
Forum: Anyone using Ubuntu on Windows (WSL)?
ubuntu wsl
updated 11 months ago by 43k • written 5.8 years ago by ▴ 250
21
votes
11
replies
2.5k
views
6 follow
Forum: Rust bioinformatics projects ideas
projects scRNA-seq RNA-seq Rust
updated 5 months ago by 22k • written 13 months ago by ▴ 150
10
votes
11
replies
4.2k
views
7 follow
Forum: What made you interested in Bioinformatics?
career
updated 11 months ago by 43k • written 6.4 years ago by ▴ 990
22
votes
11
replies
3.3k
views
Forum: ExpressionSet in the age of tidyverse
expressionset R bioconductor
updated 11 months ago by 43k • written 6.0 years ago by 13k
25
votes
11
replies
3.7k
views
6 follow
Forum: Site Use Guidelines
meta biostars Guidelines
updated 2 days ago by 43k • written 14.6 years ago by 100k
21
votes
11
replies
1.9k
views
8 follow
Forum: what I want to do after the PhD?
PhD
updated 11 months ago by 43k • written 7.1 years ago by ★ 4.3k
9
votes
11
replies
3.2k
views
7 follow
Forum: Fields with biggest demand for bioinformatics?
career
updated 11 months ago by 43k • written 5.3 years ago by ▴ 200
29
votes
10
replies
3.1k
views
Forum: How do you perform functional testing for your Bioinformatics tools?
development testing bug reliability
updated 11 months ago by 43k • written 7.1 years ago by 8.4k
26
votes
10
replies
4.4k
views
6 follow
Forum: Why not merging?
fastq sequencing
updated 11 months ago by 43k • written 6.8 years ago by 16k
7
votes
10
replies
1.8k
views
Forum: Current MCB major thinking of going into Bioinformatics
career
updated 11 months ago by 43k • written 5.7 years ago by • 0
9
votes
10
replies
17k
views
8 follow
Forum: Open Source Bioinformatics projects for beginners
Open-Source Beginners
updated 11 weeks ago by • 0 • written 6.2 years ago by ▴ 40
9
votes
10
replies
1.3k
views
6 follow
Forum: What's more clear, loops or functions?
R
updated 11 months ago by 43k • written 5.5 years ago by ▴ 110
19
votes
10
replies
1.5k
views
Forum: Pain points using commercial clouds
gpt tinybio
updated 7 months ago by ▴ 70 • written 7 months ago by ▴ 840
460 results • Page 2 of 10
Recent Votes
Comment: what is the purpose of indexing the reference genome (Kallisto)
Answer: what is the purpose of indexing the reference genome (Kallisto)
Answer: How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
Answer: How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
Answer: How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
Webinar “Learning Single-cell Atlases” with Dr. Fabian Theis, author of Scanpy on May 8
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Popular Question to sil_bioinfo ▴ 40
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Recent Replies
Comment: what is the purpose of indexing the reference genome (Kallisto) by i.sudbery 19k
Specifically: If we want to look up all the places where a 31mer is in a genome without and index, we have to compare that 31 to the first…
Answer: How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File? by benformatics 3.9k
This is way easier to do solely in R now 10 years later. library(GenomicFeatures) library(rtracklayer) ## import GTF…
Answer: what is the purpose of indexing the reference genome (Kallisto) by dsull ★ 5.8k
I'll see if I can explain things in super simple terms (albeit with some abuse of the actual technical details) to provide a conceptual und…
Answer: How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File? by Jalil Sharif ▴ 80
The updated code in R using rtracklayer for reading a gtf file. https://github.com/jalilsharif/gtf_utr_fix_r/blob/main/gencode_utr_fix_d…
Comment: Rare Disease Variant Pathway Analysis by The_PyPanda ▴ 10
Thank you LauferVA, Your advice has been useful and I now a lot to think about and read. Also, I just wanted to clarify I do not ha…
Comment: CDS vs cDNA vs transcript for mapping RNA-Seq reads by Antonio R. Franco ★ 5.1k
You cannot do it with Kallisto either. In fact, the indexation will fail in attempting to index a genome file
Comment: PCA plot by ATpoint 82k
What is your question? Add details and clarity. Ask a precise question and please avoid opening more questions on the same underlying issue…
Comment: genome assembly records not present in assembly_summary.txt by Joe 21k
If you have the accession numbers can you not use something like `eutils`?
Comment: Create a new bed file with all pairwise combinations between two other bed files by Pierre Lindenbaum 161k
> Next, I tried using bedtools window but in your original post you said > tried finding the intersection of these bed files using bedto…
Comment: How to trim transcripts using information from NCBI contamination screen report by Lada ▴ 30
Thank you very much! I tried it out! This is a handy tool useful for many different applications, and in regards to my problem - although …
Comment: Create a new bed file with all pairwise combinations between two other bed files by J • 0
Thank you for replying Pierre. I first added a third column to the SNP bed file, so that I would get a range of 1 for each SNP awk 'B…
Comment: Create a new bed file with all pairwise combinations between two other bed files by Pierre Lindenbaum 161k
> using bcftools Show us what you tried
Answer: gvcf joint calling by Jeremy Leipzig 22k
> Hi, guys, there is a question about the genomic gVCF file. I wonder > that since gVCF contains the non-var block records, why after merge…
Comment: Missing protein (VEGF-A) in String db by shalespringer • 0
Thank you for replying here; this helped me figure out why MAPK10 was missing from my results. It was also marked as a pseudogene in the En…
Comment: How to convert plink files to Hapmap Format by Sofia • 0
These are the first lines of the output: (Please is it normal to have NA in the P value column ?) CHR …
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