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460 results • Page 2 of 10
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57
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15
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6.2k
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Forum: Good practices while dealing with very large datasets
Big-Data Programming
updated 11 months ago by 43k • written 7.0 years ago by ★ 1.2k
19
votes
15
replies
2.0k
views
6 follow
Forum: Cross out line in Biostars
meta
updated 11 months ago by 43k • written 6.0 years ago by 5.3k
10
votes
15
replies
2.1k
views
Forum: How should reference genome fasta files be distributed by UCSC?
reference-genome fasta freeze ucsc-genome-browser
updated 10 months ago by 22k • written 14 months ago by ★ 1.6k
6
votes
15
replies
1.7k
views
Forum: PhD Bioinformatics on GWAS
SNP GWAS genome PHD
updated 10 months ago by 43k • written 4.2 years ago by ▴ 50
22
votes
15
replies
8.8k
views
11 follow
Forum: RNASeq user experience with Novogene?
RNA-Seq
updated 11 months ago by 43k • written 5.8 years ago by ▴ 80
28
votes
15
replies
2.2k
views
12 follow
Forum: Should bioinformaticians learn the scientific names of plants and animals?
sequence
updated 11 months ago by 43k • written 5.8 years ago by ▴ 10
23
votes
15
replies
5.1k
views
6 follow
Forum: On the usage of Golang
assembly next-gen alignment
updated 11 months ago by 43k • written 5.6 years ago by ▴ 80
19
votes
14
replies
2.1k
views
Forum: What is the amount of sequencing data produced annually?
data research sequencing
updated 7 months ago by 22k • written 7 months ago by ▴ 70
9
votes
14
replies
2.4k
views
Forum: MS Bioinformatics Project
assembly sequencing alignment R SNP
updated 11 months ago by 43k • written 6.1 years ago by ▴ 10
17
votes
14
replies
1.9k
views
10 follow
Forum: What would be the trend in next few years in NGS era?
NGS sequencing
updated 11 months ago by 43k • written 5.5 years ago by ▴ 800
73
votes
14
replies
4.3k
views
12 follow
Forum: Things you wish you knew when entering the bioinformatics field
pitfalls advice
updated 10 months ago by 43k • written 3.6 years ago by ★ 16k
5
votes
14
replies
2.0k
views
Forum: immediate help needed for genome sequence analysis of bacteria
genome miseq webinar paired-end
updated 11 months ago by 43k • written 7.1 years ago by • 0
17
votes
14
replies
3.3k
views
Forum: Asking for feedback on a Python library for computing alignments
alignment Python
updated 10 months ago by ▴ 220 • written 2.3 years ago by ▴ 60
3
votes
14
replies
2.1k
views
6 follow
Forum: Search for gene variants
next-gen
updated 11 months ago by 43k • written 5.7 years ago by 8.3k
12
votes
14
replies
2.4k
views
8 follow
Forum: Recovering bam files after unknown deletion in the storage
photorec bam sequencing
written 4.9 years ago by ▴ 70
30
votes
13
replies
3.7k
views
11 follow
Forum: what is your daily must see bioinformatics web site?
learn technology
updated 10 months ago by 43k • written 9.6 years ago by 9.7k
7
votes
13
replies
1.4k
views
Forum: How do you validate and verify your pipeline's software updates?
Validation Verification Quality-Assurance
updated 6 months ago by 43k • written 6 months ago by ▴ 10
15
votes
13
replies
4.1k
views
8 follow
Forum: Does a PhD or work experience set you up better to do bioinformatics work in industry?
advice industry phd
updated 3 months ago by 3.9k • written 17 months ago by ▴ 30
1
vote
13
replies
2.8k
views
Forum: Bioinformatics computer upgrade help
computer
updated 10 months ago by 43k • written 3.8 years ago by ▴ 50
4
votes
13
replies
1.7k
views
Forum: Looking for Participants: Meenta NovaSeq RunShare Beta
RunShare next-gen NovaSeq
updated 10 months ago by 43k • written 4.8 years ago by ▴ 10
11
votes
13
replies
4.5k
views
6 follow
Forum: NGS Data storage solutions for small organisations or big labs
big-data HTS storage NGS metagenomics
updated 11 months ago by 43k • written 4.9 years ago by ▴ 830
4
votes
12
replies
1.5k
views
Forum: publicly available data
arrayexpress GEO SRA
updated 10 months ago by 43k • written 4.6 years ago by ▴ 110
23
votes
12
replies
1.9k
views
6 follow
Forum: Whole-Exome Analysis on a Mainstream Desktop PC
NGS whole-exome
updated 10 months ago by 43k • written 3.9 years ago by 11k
12
votes
12
replies
3.0k
views
Forum: Programming interview via Skype
programming-test interview
updated 11 months ago by 43k • written 5.9 years ago by ▴ 50
26
votes
12
replies
7.5k
views
6 follow
Forum: Mapping God Found ‘Scientifically Dishonest’ By Anonymous Peer Reviewers
ngs
updated 11 months ago by ▴ 10 • written 10.5 years ago by ▴ 50
18
votes
12
replies
2.5k
views
6 follow
Forum: why GATK makes things more complicated ?
genomics
updated 11 months ago by 43k • written 5.2 years ago by ▴ 280
21
votes
12
replies
3.5k
views
Forum: How to Use Biostars Part-3: Formatting Text and Using GitHub Gists
meta how-to documentation
3 months ago by Ram 43k
12
votes
12
replies
1.4k
views
7 follow
Forum: Who qualify as a world class bioinformatician as seen in job ads
world-class bioinformatician qualification
updated 10 months ago by 43k • written 4.1 years ago by • 0
22
votes
12
replies
1.8k
views
6 follow
Forum: What were the most impactful Bioinformatics papers published this year
papers publications
updated 10 months ago by 43k • written 4.4 years ago by ▴ 520
31
votes
12
replies
947
views
7 follow
Forum: Nextflow and self-made pipelines opinion
nextflow pipeline data-analysis
8 weeks ago by karlaarz ▴ 90
25
votes
11
replies
3.7k
views
6 follow
Forum: Site Use Guidelines
meta biostars Guidelines
updated 2 days ago by 43k • written 14.6 years ago by 100k
21
votes
11
replies
2.5k
views
6 follow
Forum: Rust bioinformatics projects ideas
projects scRNA-seq RNA-seq Rust
updated 5 months ago by 22k • written 13 months ago by ▴ 150
9
votes
11
replies
3.2k
views
7 follow
Forum: Fields with biggest demand for bioinformatics?
career
updated 11 months ago by 43k • written 5.3 years ago by ▴ 200
13
votes
11
replies
4.7k
views
Forum: Anyone using Ubuntu on Windows (WSL)?
ubuntu wsl
updated 11 months ago by 43k • written 5.8 years ago by ▴ 250
21
votes
11
replies
2.0k
views
Forum: Most important International Big Data Projects: Genetics and Epigenetics
big-data
updated 11 months ago by 43k • written 5.7 years ago by ★ 9.4k
10
votes
11
replies
4.2k
views
7 follow
Forum: What made you interested in Bioinformatics?
career
updated 11 months ago by 43k • written 6.4 years ago by ▴ 990
8
votes
11
replies
1.8k
views
Forum: What makes a good bionformatics prediction service from the point of user experience?
server prediction-service
updated 11 months ago by 43k • written 6.5 years ago by ▴ 40
23
votes
11
replies
2.0k
views
9 follow
Forum: file and directory management best practices
file-management
updated 10 months ago by 43k • written 3.7 years ago by ▴ 140
22
votes
11
replies
3.3k
views
Forum: ExpressionSet in the age of tidyverse
expressionset R bioconductor
updated 11 months ago by 43k • written 6.0 years ago by 13k
21
votes
11
replies
1.9k
views
8 follow
Forum: what I want to do after the PhD?
PhD
updated 11 months ago by 43k • written 7.1 years ago by ★ 4.3k
14
votes
11
replies
6.7k
views
7 follow
Forum: Do you need a deduplication tool for FASTQ data in fastp?
deduplication fastp
updated 11 months ago by 43k • written 4.9 years ago by ★ 2.5k
18
votes
11
replies
2.4k
views
8 follow
Forum: Create a File format to store Variant
hgvs json variant
updated 11 months ago by 43k • written 5.8 years ago by ★ 2.4k
15
votes
11
replies
4.7k
views
9 follow
Forum: Methodological problems are extremely common for enrichment analysis - beware the pitfalls before you publish
GSEA DAVID enrichment-analysis -geneontology
updated 11 months ago by 43k • written 2.1 years ago by ★ 1.9k
17
votes
11
replies
981
views
Forum: How to overcome the imposter syndrome in bioinformatics?
advice
3 months ago by sil_bioinfo ▴ 40
9
votes
10
replies
17k
views
8 follow
Forum: Open Source Bioinformatics projects for beginners
Open-Source Beginners
updated 12 weeks ago by • 0 • written 6.3 years ago by ▴ 40
19
votes
10
replies
1.5k
views
Forum: Pain points using commercial clouds
gpt tinybio
updated 7 months ago by ▴ 70 • written 7 months ago by ▴ 840
9
votes
10
replies
1.7k
views
Forum: Brainstorming: applying bioinformatics NOT to public field. Ideas and perspectives
applied-sciences data-analysis
updated 11 months ago by 43k • written 6.1 years ago by ★ 1.3k
10
votes
10
replies
1.7k
views
Forum: Doctor looking fertility for a collaborator for text mining on pubmed
text-mining fertility pubmed
updated 11 months ago by 43k • written 6.4 years ago by • 0
9
votes
10
replies
1.3k
views
6 follow
Forum: What's more clear, loops or functions?
R
updated 11 months ago by 43k • written 5.5 years ago by ▴ 110
15
votes
10
replies
2.3k
views
6 follow
Forum: help identifying bioinformatics operations tools
database next-gen operations genomics
updated 11 months ago by 43k • written 5.4 years ago by ▴ 80
460 results • Page 2 of 10
Recent Votes
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix
how to combine multiple RNAseq count files into a single dataframe in R and unix
Answer: genome assembly records not present in assembly_summary.txt
Webinar “Learning Single-cell Atlases” with Dr. Fabian Theis, author of Scanpy on May 8
A: How to process (seems) Agilent microarrry data?
Comment: genome assembly records not present in assembly_summary.txt
Answer: genome assembly records not present in assembly_summary.txt
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Scholar to GenoMax 141k
Popular Question to rheab1230 ▴ 140
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Recent Replies
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix by BioinfGuru ★ 1.7k
Hi, Just thought I'd share this code snippet here for when each file contains multiple samples. I tried using lapply (as suggested by Ram)…
Comment: Low mapping rate with Salmon by i.sudbery 19k
Take your STAR alignment and sum all the counts for each gene. You can either do this by providing an annotation to STAR, or by running fea…
Comment: How to convert plink files to Hapmap Format by bk11 ★ 2.4k
If you run your data with plink 2.0, you will have ERRCODE column in your result file showing the reason behind "NA" p-value. https://www.b…
Comment: genome assembly records not present in assembly_summary.txt by sapuizait ▴ 10
jesus its in the Genbank file and I was looking at the refseq! I m such a moron - thanks for pointing it out -sorry about that :(
Comment: ScRNA data question by bk11 ★ 2.4k
I wonder how the `Vlnplots` will look if you normalize the data using `NormalizeData()` function in `Seurat`. The flat line in your plot ar…
Comment: Low mapping rate with Salmon by GenoMax 141k
> Does that mean that the reads that are not mapped to my trascriptome are not exons/coding genes? Reads are likely aligning in regions wh…
Comment: How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File? by cmdcolin ★ 3.8k
the gencode link is broken now but here is a back up of that blogpost on archive https://web.archive.org/web/20130618221342/http://gencodeg…
Comment: Low mapping rate with Salmon by Patadu94 • 0
I'll try to make the decoy file again but I remember you also told me that it is only recommended and not mandatory for running `salmon`. I…
Comment: Low mapping rate with Salmon by Patadu94 • 0
For doing this, should I just look at the log.out.file?
Answer: Heatmap and rna-seq by pinheirofabiano ▴ 10
install.packages("pheatmap") library(pheatmap) setwd("/Users/data_analysis/results") data <- read.table(file…
Comment: Low mapping rate with Salmon by Patadu94 • 0
Thanks for the link GenoMax. But I was wondering, should not alignment and mapping have a similar rate and thus be correlated? In my case I…
Answer: Create a new bed file with all pairwise combinations between two other bed files by Pierre Lindenbaum 161k
bedtools intersect \ -a <(sort -t $'\t' -k1,1 -k2,2n A.bed) \ -b <(awk '{X=250000;P=int($2);printf("%s\t%d\…
Comment: Create a new bed file with all pairwise combinations between two other bed files by J • 0
The command lines I wrote out for you are the ones I actually used. My original reference to bedtools intersect was because I assumed the b…
Comment: ScRNA data question by starswillfade ▴ 10
I just thought it was weird that considering each dot is a cell their expressions were all on a single line instead of being spread out lik…
Comment: ScRNA data question by starswillfade ▴ 10
![enter image description here][1] I am already using Seurat V5 I think. I am using SCTransform as it was recommended by the seurat page. I…
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