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84
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22
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4.6k
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12 follow
Forum: is fortran still used in bioinformatics?
FORTRAN R
updated 13 months ago by 43k • written 4.9 years ago by ▴ 190
34
votes
22
replies
2.9k
views
11 follow
Forum: Have we failed as bioinformatician in this time of COVID-19
Bioinformatician COVID-19
updated 10 months ago by 43k • written 4.0 years ago by ▴ 10
66
votes
21
replies
6.8k
views
18 follow
Forum: Is there anyone who was a pure biologist?
next-gen
updated 13 months ago by 43k • written 10.0 years ago by ▴ 100
20
votes
21
replies
2.9k
views
6 follow
Forum: Next Gen seq for Job interview
next-gen
updated 13 months ago by 43k • written 9.2 years ago by ▴ 20
29
votes
21
replies
6.2k
views
Forum: PhD vs Field application scientist in industry
career
updated 12 months ago by 43k • written 7.7 years ago by ▴ 80
35
votes
21
replies
3.1k
views
11 follow
Forum: Should you restrict use of your software based on your political views?
software
updated 19 months ago by 43k • written 8.6 years ago by 27k
17
votes
21
replies
3.5k
views
8 follow
Forum: bioinformatics thesis
thesis
updated 3.0 years ago by 21k • written 3.0 years ago by ▴ 40
67
votes
21
replies
6.1k
views
8 follow
Forum: Meta: Markdown in Biostars (now enabled)
meta
13 months ago by Ram 43k
81
votes
21
replies
5.0k
views
16 follow
Forum: [UPDATE] Thrown into a Bioinformatics Position
bioinformatics
updated 13 months ago by 43k • written 7.9 years ago by ★ 3.2k
39
votes
21
replies
13k
views
10 follow
Forum: Orientation of PE reads a review of --fr --ff and --rf meanings
RNA-Seq
updated 11 months ago by 43k • written 5.5 years ago by 8.5k
42
votes
21
replies
15k
views
10 follow
Forum: A Database Of Signatures Of Selection In The 1000 Genomes Dataset
1000genomes human selection
updated 13 months ago by 43k • written 10.2 years ago by 28k
36
votes
21
replies
19k
views
9 follow
Forum: What Is Your Favorite Conserved Protein?
protein conservation
updated 14 months ago by 43k • written 10.7 years ago by 11k
99
votes
21
replies
8.9k
views
14 follow
Forum: How Has Bioinformatics Improved Over Time?
bioinformatics
updated 14 months ago by 43k • written 11.2 years ago by ▴ 370
27
votes
21
replies
9.2k
views
9 follow
Forum: Better for my career: Degree in computer science or Phd in bioinformatics?
computer-science phd
updated 12 months ago by 43k • written 7.6 years ago by ★ 1.7k
12
votes
20
replies
3.3k
views
Forum: Starting a career in Bioinformatics
career
updated 13 months ago by 43k • written 7.9 years ago by ▴ 10
7
votes
20
replies
2.1k
views
Forum: NGS RNA-Seq Analysis Pipeline Tophat vs STAR
STAR RNA-Seq TopHat
updated 11 months ago by 43k • written 3.3 years ago by ▴ 10
91
votes
20
replies
20k
views
15 follow
Forum: Publications for individualized medicine in cancer by whole genome, exome or transcriptome sequencing
N-of-one WGS RNA-Seq next-gen Exome
updated 18 months ago by 43k • written 9.9 years ago by 20k
50
votes
20
replies
6.0k
views
18 follow
Forum: Explain bioinformatics to a 10 year old
bioinformatics
updated 13 months ago by 43k • written 8.1 years ago by ★ 1.1k
42
votes
20
replies
3.4k
views
11 follow
Forum: What are the most popular NGS tools?
ensembl NGS
updated 12 months ago by 43k • written 8.0 years ago by 23k
49
votes
20
replies
2.0k
views
10 follow
Forum: First experiences matter
first-time tolerance
updated 3 months ago by ★ 2.6k • written 3 months ago by ★ 27k
45
votes
19
replies
2.8k
views
9 follow
Forum: Where and how NGS techniques are heading for the next 5 years?
next-gen NGS
updated 11 months ago by 43k • written 6.9 years ago by 5.8k
9
votes
19
replies
8.6k
views
Forum: Careful!! Velvet Generates Different Assemblies From The Same Input Data And Same Input Parameters With Openmp Enabled.
velvet genome
updated 14 months ago by 43k • written 10.4 years ago by ▴ 660
47
votes
19
replies
14k
views
10 follow
Forum: Is Software Development a key part of Bioinformatics?
Software-Development
updated 20 months ago by 43k • written 9.3 years ago by ▴ 70
49
votes
19
replies
7.3k
views
13 follow
Forum: Why Are There No Proteomics Questions In Biostar?
proteomics biostars
updated 14 months ago by 43k • written 13.5 years ago by ▴ 410
56
votes
19
replies
4.1k
views
9 follow
Forum: How to apply for a job
resume cover-letter application CV
updated 11 months ago by 43k • written 6.7 years ago by 23k
34
votes
19
replies
7.9k
views
12 follow
Forum: Programming Language In Bioinformatics
perl python
updated 14 months ago by 43k • written 11.0 years ago by ▴ 260
38
votes
19
replies
10k
views
10 follow
Forum: Kallisto New RNA-seq quantification method discussion
RNA-Seq
updated 12 months ago by 43k • written 8.0 years ago by ▴ 550
35
votes
19
replies
3.7k
views
6 follow
Forum: For the general public seeking advice from this community
diagnosis rare-disease
updated 19 months ago by 43k • written 8.6 years ago by 11k
50
votes
19
replies
4.9k
views
7 follow
Forum: Proposal: Biostar Wants To Run Your Ads. Feedback Requested.
biostars
updated 14 months ago by 43k • written 11.2 years ago by 100k
27
votes
19
replies
4.6k
views
10 follow
Forum: Google genomics cloud platform ?
genome cloud
updated 13 months ago by 43k • written 8.8 years ago by 12k
35
votes
19
replies
4.0k
views
Forum: Crowd Sourcing The 'Analysis' Of The 'Big Foot' Genome
bigfoot genome
updated 14 months ago by 43k • written 11.2 years ago by 4.5k
57
votes
19
replies
28k
views
6 follow
Forum: Attention: Bowtie2 And Multiple Hits
next-gen bowtie mapping
updated 14 months ago by 43k • written 11.5 years ago by 11k
30
votes
19
replies
10k
views
6 follow
Forum: Programming language use distribution from recent programs / articles
programming-language programs
updated 11 months ago by 43k • written 7.0 years ago by 11k
70
votes
19
replies
5.6k
views
13 follow
Forum: Thrown Into A Bioinformatics Position
computational-analysis
updated 21 months ago by 43k • written 8.6 years ago by ▴ 170
29
votes
18
replies
2.7k
views
12 follow
Forum: what are the big questions in bioinformatics field nowadays
genome
updated 10 months ago by 43k • written 4.2 years ago by ▴ 20
23
votes
18
replies
1.5k
views
Forum: Versioning of Blast DB and Reproducible Research
database blast reproducible-research
updated 10 months ago by 43k • written 3.9 years ago by ▴ 60
38
votes
18
replies
4.2k
views
11 follow
Forum: Should We Have A Biostar Conference Or Meeting?
conference biostars
updated 14 months ago by 43k • written 10.8 years ago by 54k
23
votes
18
replies
2.6k
views
9 follow
Forum: How to imagine the future of bioinformatics?
bioinformatics
updated 12 months ago by 43k • written 7.6 years ago by ▴ 570
13
votes
18
replies
3.2k
views
6 follow
Forum: Bioinformatics independent study group
Proteomic RNA-Seq Genomic
updated 11 months ago by 43k • written 6.7 years ago by ★ 1.3k
21
votes
18
replies
1.7k
views
Forum: Is it safe to keep our data in github
github
updated 10 months ago by 43k • written 4.0 years ago by ▴ 70
29
votes
18
replies
3.3k
views
7 follow
Forum: Should I quit PhD program?
education career research phd
updated 11 months ago by 43k • written 6.0 years ago by ▴ 30
52
votes
18
replies
6.3k
views
14 follow
Forum: Do You Feel Good Or Bad About Putting Your Photo At Biostar?
meta biostars
updated 14 months ago by 43k • written 10.6 years ago by ★ 1.1k
36
votes
18
replies
8.1k
views
Forum: How Does Genomebrowse Free Genome Browser From Golden Helix Compare?
visualization genome-browser
updated 3.0 years ago by 20 • written 11.1 years ago by ▴ 140
72
votes
18
replies
7.5k
views
17 follow
Forum: What Is One Book Every Bioinformatician Should Read?
books
updated 14 months ago by 43k • written 10.8 years ago by 8.3k
33
votes
17
replies
3.4k
views
7 follow
Forum: What bioinformatics tools/software do not get enough recognition?
software
updated 13 months ago by 43k • written 9.5 years ago by 24k
21
votes
17
replies
9.2k
views
7 follow
Forum: In YOUR personal opinion, who are the top 5 bioinformatics researchers in NGS in 21st century?
next-gen
updated 12 months ago by 43k • written 7.3 years ago by ▴ 10
29
votes
17
replies
2.2k
views
6 follow
Forum: Can Biostars use question template like Github issue/PR?
meta biostars template
updated 12 months ago by 43k • written 7.2 years ago by 8.4k
13
votes
17
replies
4.2k
views
6 follow
Forum: Advice for undergraduate bioinformatics learners
advice
updated 20 months ago by 43k • written 8.4 years ago by ▴ 10
13
votes
17
replies
4.8k
views
8 follow
Forum: A Universal/Common Biorep System
reputation
updated 14 months ago by 43k • written 10.4 years ago by 20k
26
votes
17
replies
2.3k
views
9 follow
Forum: GalaxyProject, why and why not?
galaxy sequencing pipeline
updated 12 months ago by 43k • written 8.2 years ago by ▴ 50
1,000 results • Page 3 of 20
Recent Votes
cellular niches analysis with spatial transcriptome data in Seurat and Bioconductor
Answer: Seurat merge and batch correction
Comment: How to convert plink files to Hapmap Format
Comment: How to convert plink files to Hapmap Format
Comment: How to convert plink files to Hapmap Format
Comment: How to convert plink files to Hapmap Format
Comment: How to convert plink files to Hapmap Format
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Recent Replies
Comment: Create a new bed file with all pairwise combinations between two other bed files by J • 0
Thank you for replying Pierre. I first added a third column to the SNP bed file, so that I would get a range of 1 for each SNP awk 'B…
Comment: Create a new bed file with all pairwise combinations between two other bed files by Pierre Lindenbaum 161k
> using bcftools Show us what you tried
Answer: gvcf joint calling by Jeremy Leipzig 22k
> Hi, guys, there is a question about the genomic gVCF file. I wonder > that since gVCF contains the non-var block records, why after merge…
Comment: Missing protein (VEGF-A) in String db by shalespringer • 0
Thank you for replying here; this helped me figure out why MAPK10 was missing from my results. It was also marked as a pseudogene in the En…
Comment: How to convert plink files to Hapmap Format by Sofia • 0
These are the first lines of the output: (Please is it normal to have NA in the P value column ?) CHR …
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie by atowns21 • 0
So I used the barcodes that I created (combos of positions 1-10 and 11-20) and I obtained similar alignment stats as the paper I pulled the…
Comment: How to convert plink files to Hapmap Format by Sofia • 0
Thank you so much, it actually worked!
Answer: Rare Disease Variant Pathway Analysis by LauferVA 4.2k
Hi @efc1e545 , First a caveat. the information we most need in order to help guide you to a successful conclusion is not provided in thi…
Answer: Sequence read length shorter than flow cell specification by swbarnes2 14k
The company probably had you share your run with someone who needed the extra bases. So you get the extra bases free. Just use them unle…
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix by Ram 43k
Thank you, the `csvtk spread` is super useful. I usually import into R using `lapply` then `Reduce` using `merge` but this might be easier.
Comment: Annovar using R package by DKA ▴ 40
Thank you for your guidance. The thing is that I am unfamiliar with using such environments, unfortunately.
Comment: Treatment VS Control in Single Cell RNAseq analysis by ATpoint 82k
Open a new question, with details.
Comment: Normalize scRNAseq data to housekeeping genes to compare several datasets by ATpoint 82k
I recommend https://bioconductor.org/books/3.18/OSCA.basic/normalization.html as well as the "advanced" section in this book.
Comment: Why gatk VariantAnnotator required bam and coverage files by QX • 0
thank you!
Comment: Treatment VS Control in Single Cell RNAseq analysis by kilcdincer ▴ 10
Hello, I have more or less same experimental setting and was wondering how you proceeded with your analysis? Can I reach its GitHub reposit…
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