12 days ago by
Rafael Soler
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updated 8 months ago by
Matthias Zepper
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written 8 months ago by
A_heath
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updated 17 months ago by
Ram
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written 8.8 years ago by
trakhtenberg
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updated 21 months ago by
Ram
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written 3.4 years ago by
Begonia_pavonina
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updated 18 months ago by
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written 18 months ago by
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5.2 years ago by
ishackm
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3.1 years ago by
lokeshp14cs24
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5.0 years ago by
plebaninora
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updated 5 months ago by
Ram
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written 7.9 years ago by
QVINTVS_FABIVS_MAXIMVS
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5.9 years ago by
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updated 7.0 years ago by
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written 7.0 years ago by
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4.1 years ago by
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4.8 years ago by
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updated 14 months ago by
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written 14 months ago by
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5.1 years ago by
mhyunjunkang
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9.3 years ago by
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updated 9.5 years ago by
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written 10.2 years ago by
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7.2 years ago by
Gabe Anderson
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updated 9 months ago by
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written 8.0 years ago by
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2.4 years ago by
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updated 3.3 years ago by
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written 3.3 years ago by
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Are you sure this is appropriate for bulk RNASeq? I thought intronic reads for single cell were only counted in nuclear preps.
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swbarnes2
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Are you sure these samples are 10x? How likey is it that the sequencing group messed up and ran the samples wrongly?
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are they distinct transcript IDs but the same gene ID? can you provide any additional context?
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that didn't work either
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Hi @kermit and prash (and others)!
Nice discussion. I wanted to offer some **biological rationales** in addition to the technical reasons …
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There is no `:` after `rule fastqc`
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sorry that was a typo while i was copying my code! I still get the same error
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> fastq.gz".
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how can you know the primer is not bind to other gene site? how can you desgin an experiment? I use the FISH to identify the gene, how abo…
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sorry for my late reply, I am a student, my profossor ask me to dsgin a primer for the specific cDNA, I have solved now, thanks for your re…
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In this case filtering for quality might help correct for this overattributing feature?
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Did you see this guidance from ABySS manual: https://github.com/bcgsc/abyss#optimizing-the-parameters-k-and-kc
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