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121,618 results • Page 625 of 2433
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1
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852
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Should do Seurat NormalizeData after Cellranger aggr?
scRNA
4.1 years ago by hq.huang11.6 ▴ 10
4
votes
4
replies
4.2k
views
Error: cannot allocate vector of size 22.3 Gb
wgcna memory error R
updated 4.1 years ago by 3.9k • written 4.1 years ago by ▴ 50
2
votes
3
replies
1.4k
views
Scaffolding of bacterial genome assemblies
scaffolding genome annotation bacterial assembly
updated 4.1 years ago by ▴ 600 • written 4.1 years ago by • 0
1
vote
0
replies
446
views
What are the standard strategies for combining multiple publicly avaiable datasets of microarray on the same platform and chips design?
mircoarray Integrative-metaanalysis
4.1 years ago by amay ▴ 10
0
votes
0
replies
593
views
SNP calling in the chrX/Y of HG002/NA24385
son Ashkenazi HG002 NA24385
4.1 years ago by cll710426 • 0
2
votes
7
replies
3.5k
views
trouble with 'too short' reads using STAR. each mate maps fine, but the paired end mapping does not
RNA-Seq alignment
updated 4.1 years ago by 15k • written 4.4 years ago by ▴ 20
0
votes
1
reply
1.3k
views
Plink files from genome studio how
plink Genomestudio
updated 4.1 years ago by 152k • written 4.1 years ago by • 0
0
votes
0
replies
694
views
Merlin haplotyping problem
pedigree inference snps haplotype merlin
4.1 years ago by S. Roa ▴ 10
1
vote
3
replies
966
views
Low percentage explained by PCs
PCA SNPRelate
updated 4.1 years ago by 89k • written 4.1 years ago by • 0
0
votes
0
replies
815
views
GO enrichment analysis in Blast2GO
Ontology Gene Enrichment Blast2GO
4.1 years ago by a.sugi30 • 0
0
votes
1
reply
2.6k
views
Get Rs Number Based On Chromosome and Position in GWAS summary statistics
Annovar rsID geneticposition
updated 4.1 years ago by 89k • written 4.2 years ago by • 0
0
votes
3
replies
1.3k
views
Normalisation of RNA-seq data for coexpression analysis
RNA-seq coexpression normalisation
updated 4.1 years ago by 89k • written 4.2 years ago by • 0
1
vote
7
replies
1.7k
views
For loop STAR not working
RNA-Seq STAR
updated 2.1 years ago by 45k • written 4.1 years ago by ▴ 30
0
votes
1
reply
768
views
How to calculate the SNP score?
SNP SCORE
updated 4.1 years ago by 45k • written 4.1 years ago by • 0
1
vote
2
replies
1.2k
views
Look at Different Locus in the Bam File
Genome HG38 Rna-Seq Nanopore Sequencing
4.1 years ago by santos48 ▴ 40
0
votes
4
replies
934
views
Mapping gene list with chromosome location
R
updated 4.1 years ago by 45k • written 4.1 years ago by ▴ 70
0
votes
2
replies
871
views
Unzipped output files: fasta files in python
fasta python
updated 4.1 years ago by 45k • written 4.1 years ago by ▴ 20
0
votes
8
replies
2.2k
views
Inconsistency in SNP calls using bcftools merge for multiple vcf files
bcftools vcf snp
4.1 years ago by evafinegan • 0
2
votes
1
reply
635
views
Incrementally updating index files for Blast and for Bowtie2
blast index bowtie
updated 4.1 years ago by 152k • written 4.1 years ago by ▴ 30
1
vote
4
replies
2.0k
views
RNA-seq differential analysis with N batches and a technical replicate in each batch
RNA-Seq deseq2 differential analysis edger
updated 4.1 years ago by • 0 • written 5.5 years ago by • 0
0
votes
1
reply
1.6k
views
Five-parameter logistic (5PL) regression - Python or R ?
Bioassay 5PL Python R
updated 4.1 years ago by ▴ 90 • written 4.1 years ago by • 0
4
votes
2
replies
1.1k
views
Presence of proteins in a group of organisms
proteins restriction BLAST
updated 4.1 years ago by 35k • written 4.1 years ago by ▴ 30
2
votes
2
replies
876
views
Unable to read fasta file
fasta
updated 2.3 years ago by 45k • written 4.1 years ago by • 0
1
vote
4
replies
1.0k
views
What is the best short reader to very large reference fasta?
mapping alignment fastq bowtie2 bwa read
4.1 years ago by O.rka ▴ 750
7
votes
5
replies
1.9k
views
On whether or not to normalize scRNAseq expression like qPCR
single-cell qpcr rnaseq
updated 4.1 years ago by 22k • written 4.1 years ago by ▴ 380
2
votes
16
replies
8.5k
views
11 follow
PSMC: fq2psmc conversion problems
PSMC conversion fastq psmcfa
updated 3.2 years ago by ▴ 40 • written 6.8 years ago by ★ 3.0k
3
votes
11
replies
2.5k
views
How to associate a gene to one general function?
gene function
4.1 years ago by Francois Piumi ▴ 70
0
votes
2
replies
1.1k
views
BED Heatmap
ChIP-seq
updated 4.1 years ago by 45k • written 4.1 years ago by ▴ 80
3
votes
7
replies
3.7k
views
6 follow
PSMC Illegal division by zero
genome software error sequence
3.6 years ago by YocelynGG ▴ 70
2
votes
5
replies
3.2k
views
snakemake job status monitoring
snakemake pipelines
4.1 years ago by Eugene A ▴ 190
1
vote
3
replies
1.2k
views
Read alignment anomaly
igv reads rna-seq
4.1 years ago by daewowo ▴ 80
4
votes
3
replies
1.0k
views
which genes causes breast cancer?
Breast Cancer
updated 4.1 years ago by 56k • written 4.1 years ago by • 0
4
votes
4
replies
1.2k
views
analysis of novel SARS-CoV-2 genomes
processing phylogenetics analysis COVID19
updated 4.1 years ago by 152k • written 4.1 years ago by ▴ 140
6
votes
6
replies
2.6k
views
How to generate GTF file from BLAST output?
GTF
4.1 years ago by mathavanbioinfo ▴ 80
13
votes
12
replies
3.0k
views
How to determine reasonable expression threshold for of a gene from scRNAseq to imply its biological significance
statistics single-cell rnaseq
4.1 years ago by gundalav ▴ 380
0
votes
0
replies
549
views
When to use Pharmacophore and QSAR, in which conditions.
design Drug
4.1 years ago by Sachidanand • 0
38
votes
8
replies
55k
views
11 follow
Adding Read Groups To Bam Files
bam gatk samtools bwa
updated 4.1 years ago by 12k • written 13.1 years ago by 12k
1
vote
8
replies
1.9k
views
Seqff.r script is giving error while running
R
4.1 years ago by smrutimayipanda ▴ 20
1
vote
1
reply
3.4k
views
Interpret Clustal Omega Distance/Percent Identity Output for Multiple Proteins
Omega Clustal similarity sequence
updated 4.1 years ago by ★ 29k • written 4.1 years ago by ▴ 50
0
votes
0
replies
557
views
Detecting genetic mutation with raw data
mutation Krt1 genetic hyperkeratosis krt10
4.1 years ago by Amanda • 0
4
votes
5
replies
1.5k
views
Are there any statistical method is suitable to solve
z-test
updated 2.6 years ago by 45k • written 4.1 years ago by ▴ 120
2
votes
4
replies
44k
views
python 3.8 'No module named pip'
rna-seq
updated 4.1 years ago by ▴ 10 • written 5.0 years ago by • 0
0
votes
1
reply
862
views
Downloading bacterial reference genome
reference genome
updated 4.1 years ago by 8.7k • written 4.1 years ago by • 0
0
votes
1
reply
884
views
Where to get the human mitochondiral reference genome?
GTF human genome mitochondiral
updated 4.1 years ago by 152k • written 4.1 years ago by ▴ 80
2
votes
4
replies
1.2k
views
Advantages of split by chromosome?
cnv split gatk
updated 4.1 years ago by 166k • written 4.1 years ago by ▴ 20
5
votes
4
replies
3.3k
views
distance matrix as input in R clustering functions ?
k-medioids-genetic-distances-mash R Clustering
updated 4.1 years ago by 27k • written 4.1 years ago by ▴ 140
0
votes
0
replies
563
views
missing calls for a vcf file
SNP
4.1 years ago by evafinegan • 0
5
votes
7
replies
2.7k
views
How to find the functions of Reactome pathways?
Pathways reactome pathways DAVID
updated 4.1 years ago by ▴ 580 • written 7.2 years ago by ▴ 260
0
votes
0
replies
680
views
ANOVA testing
R ANOVA p-values
4.1 years ago by ehruan ▴ 10
1
vote
1
reply
1.5k
views
Plink error: Variant names are limited to 16000 characters.
plink imputation
updated 4.1 years ago by 12k • written 4.1 years ago by ▴ 10
121,618 results • Page 625 of 2433
Recent Votes
[Deprecated] Fast download of FASTQ files from the European Nucleotide Archive (ENA)
RNA-seq low quality sample in downstream analysis
RNA-seq low quality sample in downstream analysis
Comment: RNA-seq low quality sample in downstream analysis
Comment: RNA-seq low quality sample in downstream analysis
Answer: Fixing MGI Sequence Headers - FASTQ Format
Fixing MGI Sequence Headers - FASTQ Format
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Comment: Does edgeR supports random effects? by 406339165 ▴ 10
Hi all, Thank you for the helpful suggestions. In my dataset, I observed a significant effect of Sex, so I subset the data to include only…
Comment: Looking for neanderthal genomes to download by RD ▴ 20
Hey @severalorks, Were you able to figure it out? I'm curious how you built the consensus sequences and aligned them to human proteins. I n…
Comment: How to pull out protein sequences from from BAM files containing genomic alignme by RD ▴ 20
Hey @Julia92796, Were you able to figure it out? I'm curious how you built the consensus sequences and aligned them to human proteins. I no…
Comment: STAR alignment to small fasta file failing by GenoMax 152k
What is the length of fasta reference sequences?
Comment: Significance of the term "tile" in sequencing? by GenoMax 152k
What kind of sequencer is this data from?
Comment: STAR aligned BAM files by Ram 45k
> but it seems that i cannot directly use these BAM files in STAR. You most definitely can.
Answer: STAR aligned BAM files by Ram 45k
You cannot use STAR to get isoform level counts, only gene level counts and you can do that using an input BAM. For transcript level counts…
Comment: Limma model gives weird results by leranwangcs ▴ 150
Sorry it takes so long to come back to this. Let me refine my question: 1. The abundance table is a 2313 viral ORFs by 306 samples table, w…
Answer: How do I generate synthetic 16S RNA gene sequences? by Mensur Dlakic ★ 29k
Why do you need to augment 16S rRNA sequences, given that tens of millions are already available? If your main reason is sequence diversity…
Comment: Inverted TSS Enrichment Plot by nmalexan • 0
Another example![enter image description here][1] [1]: /media/images/9fea51dc-fd45-4fe6-b018-f3399786
Comment: Inverted TSS Enrichment Plot by nmalexan • 0
Excellent. Just to confirm sometimes I will have a peak like I had before but with some subtle secondary and tertiary peaks. These seem to …
Comment: Annotation comparison files on NCBI of gene assembly by GenoMax 152k
> Would a separate whole transcriptome data independent of the Visium-HD data be necessary? My guess is that whether an assembled transcrip…
Answer: undefined chro name in vcf header by GenoMax 152k
See the explanation here: https://genome.ucsc.edu/FAQ/FAQdownloads#download10
Comment: how extract CpG methylatiuon calls from nanopore long sequencing bam file ? by GenoMax 152k
Please do not repost problems with a solution in separate threads. If you are having an issue continue in the same thread using the `ADD CO…
Answer: Fixing MGI Sequence Headers - FASTQ Format by GenoMax 152k
Give this a try: https://sagc-bioinformatics.github.io/mgikit/reformat
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