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34
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22
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2.9k
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11 follow
Forum: Have we failed as bioinformatician in this time of COVID-19
Bioinformatician COVID-19
updated 10 months ago by 43k • written 4.0 years ago by ▴ 10
27
votes
22
replies
5.7k
views
8 follow
Forum: Recommendations of Linux distros for beginners in bioinformatics
training linux
updated 11 months ago by 43k • written 6.4 years ago by ▴ 810
17
votes
21
replies
3.5k
views
8 follow
Forum: bioinformatics thesis
thesis
updated 3.0 years ago by 21k • written 3.1 years ago by ▴ 40
27
votes
21
replies
9.3k
views
9 follow
Forum: Better for my career: Degree in computer science or Phd in bioinformatics?
computer-science phd
updated 13 months ago by 43k • written 7.6 years ago by ★ 1.7k
35
votes
21
replies
3.1k
views
11 follow
Forum: Should you restrict use of your software based on your political views?
software
updated 19 months ago by 43k • written 8.6 years ago by 27k
36
votes
21
replies
19k
views
9 follow
Forum: What Is Your Favorite Conserved Protein?
protein conservation
updated 14 months ago by 43k • written 10.7 years ago by 11k
39
votes
21
replies
14k
views
10 follow
Forum: Orientation of PE reads a review of --fr --ff and --rf meanings
RNA-Seq
updated 11 months ago by 43k • written 5.5 years ago by 8.6k
29
votes
21
replies
6.3k
views
Forum: PhD vs Field application scientist in industry
career
updated 13 months ago by 43k • written 7.7 years ago by ▴ 80
81
votes
21
replies
5.1k
views
16 follow
Forum: [UPDATE] Thrown into a Bioinformatics Position
bioinformatics
updated 13 months ago by 43k • written 7.9 years ago by ★ 3.2k
42
votes
21
replies
15k
views
10 follow
Forum: A Database Of Signatures Of Selection In The 1000 Genomes Dataset
1000genomes human selection
updated 14 months ago by 43k • written 10.2 years ago by 28k
66
votes
21
replies
6.8k
views
18 follow
Forum: Is there anyone who was a pure biologist?
next-gen
updated 14 months ago by 43k • written 10.0 years ago by ▴ 100
99
votes
21
replies
8.9k
views
14 follow
Forum: How Has Bioinformatics Improved Over Time?
bioinformatics
updated 14 months ago by 43k • written 11.3 years ago by ▴ 370
20
votes
21
replies
2.9k
views
6 follow
Forum: Next Gen seq for Job interview
next-gen
updated 13 months ago by 43k • written 9.2 years ago by ▴ 20
67
votes
21
replies
6.1k
views
8 follow
Forum: Meta: Markdown in Biostars (now enabled)
meta
13 months ago by Ram 43k
12
votes
20
replies
3.3k
views
Forum: Starting a career in Bioinformatics
career
updated 13 months ago by 43k • written 7.9 years ago by ▴ 10
49
votes
20
replies
2.1k
views
10 follow
Forum: First experiences matter
first-time tolerance
updated 3 months ago by ★ 2.6k • written 4 months ago by ★ 27k
42
votes
20
replies
3.4k
views
11 follow
Forum: What are the most popular NGS tools?
ensembl NGS
updated 13 months ago by 43k • written 8.1 years ago by 23k
91
votes
20
replies
20k
views
15 follow
Forum: Publications for individualized medicine in cancer by whole genome, exome or transcriptome sequencing
N-of-one WGS RNA-Seq next-gen Exome
updated 19 months ago by 43k • written 10.0 years ago by 20k
7
votes
20
replies
2.1k
views
Forum: NGS RNA-Seq Analysis Pipeline Tophat vs STAR
STAR RNA-Seq TopHat
updated 11 months ago by 43k • written 3.3 years ago by ▴ 10
50
votes
20
replies
6.0k
views
18 follow
Forum: Explain bioinformatics to a 10 year old
bioinformatics
updated 14 months ago by 43k • written 8.2 years ago by ★ 1.1k
49
votes
19
replies
7.3k
views
13 follow
Forum: Why Are There No Proteomics Questions In Biostar?
proteomics biostars
updated 14 months ago by 43k • written 13.5 years ago by ▴ 410
35
votes
19
replies
3.8k
views
6 follow
Forum: For the general public seeking advice from this community
diagnosis rare-disease
updated 20 months ago by 43k • written 8.6 years ago by 11k
27
votes
19
replies
4.6k
views
10 follow
Forum: Google genomics cloud platform ?
genome cloud
updated 13 months ago by 43k • written 8.9 years ago by 12k
57
votes
19
replies
28k
views
6 follow
Forum: Attention: Bowtie2 And Multiple Hits
next-gen bowtie mapping
updated 14 months ago by 43k • written 11.5 years ago by 11k
38
votes
19
replies
10k
views
10 follow
Forum: Kallisto New RNA-seq quantification method discussion
RNA-Seq
updated 13 months ago by 43k • written 8.1 years ago by ▴ 550
47
votes
19
replies
14k
views
10 follow
Forum: Is Software Development a key part of Bioinformatics?
Software-Development
updated 20 months ago by 43k • written 9.3 years ago by ▴ 70
9
votes
19
replies
8.6k
views
Forum: Careful!! Velvet Generates Different Assemblies From The Same Input Data And Same Input Parameters With Openmp Enabled.
velvet genome
updated 14 months ago by 43k • written 10.5 years ago by ▴ 660
35
votes
19
replies
4.0k
views
Forum: Crowd Sourcing The 'Analysis' Of The 'Big Foot' Genome
bigfoot genome
updated 14 months ago by 43k • written 11.2 years ago by 4.5k
30
votes
19
replies
10k
views
6 follow
Forum: Programming language use distribution from recent programs / articles
programming-language programs
updated 11 months ago by 43k • written 7.0 years ago by 11k
70
votes
19
replies
5.6k
views
13 follow
Forum: Thrown Into A Bioinformatics Position
computational-analysis
updated 22 months ago by 43k • written 8.6 years ago by ▴ 170
34
votes
19
replies
7.9k
views
12 follow
Forum: Programming Language In Bioinformatics
perl python
updated 14 months ago by 43k • written 11.0 years ago by ▴ 260
56
votes
19
replies
4.1k
views
9 follow
Forum: How to apply for a job
resume cover-letter application CV
updated 11 months ago by 43k • written 6.8 years ago by 23k
50
votes
19
replies
4.9k
views
7 follow
Forum: Proposal: Biostar Wants To Run Your Ads. Feedback Requested.
biostars
updated 14 months ago by 43k • written 11.2 years ago by 100k
45
votes
19
replies
2.9k
views
9 follow
Forum: Where and how NGS techniques are heading for the next 5 years?
next-gen NGS
updated 11 months ago by 43k • written 6.9 years ago by 5.8k
29
votes
18
replies
2.7k
views
12 follow
Forum: what are the big questions in bioinformatics field nowadays
genome
updated 11 months ago by 43k • written 4.2 years ago by ▴ 20
72
votes
18
replies
7.5k
views
17 follow
Forum: What Is One Book Every Bioinformatician Should Read?
books
updated 14 months ago by 43k • written 10.8 years ago by 8.4k
21
votes
18
replies
1.8k
views
Forum: Is it safe to keep our data in github
github
updated 10 months ago by 43k • written 4.0 years ago by ▴ 70
38
votes
18
replies
4.2k
views
11 follow
Forum: Should We Have A Biostar Conference Or Meeting?
conference biostars
updated 14 months ago by 43k • written 10.9 years ago by 54k
36
votes
18
replies
8.2k
views
Forum: How Does Genomebrowse Free Genome Browser From Golden Helix Compare?
visualization genome-browser
updated 3.1 years ago by 20 • written 11.1 years ago by ▴ 140
23
votes
18
replies
2.6k
views
9 follow
Forum: How to imagine the future of bioinformatics?
bioinformatics
updated 13 months ago by 43k • written 7.6 years ago by ▴ 570
29
votes
18
replies
3.3k
views
7 follow
Forum: Should I quit PhD program?
education career research phd
updated 11 months ago by 43k • written 6.0 years ago by ▴ 30
52
votes
18
replies
6.3k
views
14 follow
Forum: Do You Feel Good Or Bad About Putting Your Photo At Biostar?
meta biostars
updated 14 months ago by 43k • written 10.6 years ago by ★ 1.1k
13
votes
18
replies
3.2k
views
6 follow
Forum: Bioinformatics independent study group
Proteomic RNA-Seq Genomic
updated 11 months ago by 43k • written 6.7 years ago by ★ 1.3k
23
votes
18
replies
1.5k
views
Forum: Versioning of Blast DB and Reproducible Research
database blast reproducible-research
updated 10 months ago by 43k • written 4.0 years ago by ▴ 60
26
votes
17
replies
2.3k
views
9 follow
Forum: GalaxyProject, why and why not?
galaxy sequencing pipeline
updated 13 months ago by 43k • written 8.2 years ago by ▴ 50
29
votes
17
replies
2.3k
views
6 follow
Forum: Can Biostars use question template like Github issue/PR?
meta biostars template
updated 12 months ago by 43k • written 7.2 years ago by 8.5k
42
votes
17
replies
38k
views
15 follow
Forum: List Of Genome Sequencing Centers/Institutes
genomics institutes
updated 14 months ago by 43k • written 11.2 years ago by 19k
13
votes
17
replies
4.2k
views
6 follow
Forum: Advice for undergraduate bioinformatics learners
advice
updated 21 months ago by 43k • written 8.4 years ago by ▴ 10
9
votes
17
replies
3.0k
views
Forum: “Systematic pattern of excess success"
paper statistics
updated 13 months ago by 43k • written 9.4 years ago by 3.5k
38
votes
17
replies
3.2k
views
7 follow
Forum: Why does bioinformatics use a special format like a BAM file instead of a database like SQLite?
sam bam samtools
updated 10 months ago by 43k • written 3.7 years ago by ▴ 250
1,000 results • Page 3 of 20
Recent Votes
Comment: Help with choosing a model species for Augustus for a de novo assembled genome.
A: After read mapping, count exons or genes?
After read mapping, count exons or genes?
Merging a large number of VCF files
A: Merging a large number of VCF files
A: pulling snps from vcf file using ids
pulling snps from vcf file using ids
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Recent Awards • All
Popular Question to Thanujay S • 0
Popular Question to SHN ▴ 40
Popular Question to Rob ▴ 170
Scholar to GenoMax 142k
Popular Question to curious ▴ 750
Popular Question to M. ▴ 30
Student to carolofharvest ▴ 30
Recent Replies
Comment: Segmentation error while using a tool by Thanujay S • 0
Hey @Ruqaiya! Can you please give more details on why smalt is opted here? According to what I can see, the tool has become obsolete! Why …
Comment: Extracting mutation status from MAF files - TCGA by Thanujay S • 0
Hey! Can you explain the question further? What have you tried?
Comment: Downloading older version of a tool by Ruqaiya • 0
I tried the 2nd option only. I got the files of older version from the author but that version isn't working on my WSL, it's showing Segmen…
Comment: filtering before dada2 by GenoMax 142k
> I encountered a significant decrease in the percentage of merged reads. You can check with `bbmerge.sh` from BBMap suite to see how many…
Comment: HCL database download by GenoMax 142k
Isn't the download link available above the plot: http://bis.zju.edu.cn/HCL/data/DGE/Adult-Transverse-Colon1_dge.txt.gz
Comment: fastq screen aligner no specified. by GenoMax 142k
You need to change this configuration file to point it to the location where the aligner executable can be found. So as an example followi…
Answer: Design matrix Differential expression analysis by Gordon Smyth ★ 7.1k
The expression levels do not change but the way that you have parametrized the expression levels does change. The two design matrices give…
Comment: The GDC Legacy Archive is retiring soon. by lcordeiro ▴ 40
Hi Zhenyu, do you happen to know how I can download metadata for some of the legacy files? I downloaded several IDATs from the legacy data …
Answer: Force PLINK2 to neglect chrX as the sex chromosome by chrchang523 10k
(cross-posted from https://groups.google.com/g/plink2-users/c/Qphx_6BpPII ) You need to use --chr-set, not --allow-extra-chr, to specify…
Answer: Easy way to find out which allele is minor allele from bed file? by chrchang523 10k
This question does not make sense. If you care about allele-order "information [being] lost in downstream applications", REF/ALT is the ob…
Answer: visualize GSEA by bk11 ★ 2.4k
I have made some edits in your code basically changing the position of text and commented out the line `data$text_pos <- ifelse(data$NES > …
Comment: Differential Expression Analysis using DESeq2 in R by swbarnes2 14k
Pictures of code are useless. You were told to paste the code and use the 0101 button. Can you explain why you decided not to do that? If…
Comment: Differential Expression Analysis using DESeq2 in R by Erina • 0
![Here is the code I am using for the analysis][1] [1]: /media/images/8a511904-6ae8-47f5-b647-ee15b02b
Answer: Clarification regarding SAM flags "mate reverse strand" (flag 16/0x10) and "read by swbarnes2 14k
In paired end sequencing, every read has a mate, and the flags all together tell you not only what is up with the read you are looking at, …
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix by Mbofire • 0
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