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1,000 results • Page
1 of 20
Sort: replies
Rank
Views
Votes
Replies
41
votes
55
replies
12k
views
6 follow
hash function for DNA sequences
hash
hashing
DNA
8.1 years ago by
midox
▴ 290
4
votes
45
replies
7.8k
views
SAM file wrong? help with validatesamfile
EXOME
updated 13 months ago by
Ram
43k • written 7.9 years ago by
cristina_sabiers
▴ 110
19
votes
43
replies
5.6k
views
Annotation of huge number of CNV files
CNV annotation TCGA
5.7 years ago by
nazaninhoseinkhan
▴ 520
16
votes
38
replies
17k
views
Differential gene Analysis by Limma
Limma
Microarray
R
updated 2.0 years ago by
Ram
43k • written 9.2 years ago by
Mo
▴ 920
17
votes
37
replies
4.8k
views
No alias or index file found for protein database
next-gen
software error
sequence
updated 4.9 years ago by
Biostar
20 • written 5.1 years ago by
williamsbrian5064
▴ 510
5
votes
35
replies
3.7k
views
Reduce set of chromosomes in Pangenome graph
pangenome
vg
updated 9 months ago by
Jordan M Eizenga
▴ 460 • written 10 months ago by
anivlete
• 0
3
votes
32
replies
3.4k
views
fastqc report for degradome reads
fastqc
adaptor
6.1 years ago by
Sam
▴ 150
4
votes
30
replies
2.4k
views
SNPs and DEL/MNP in the same position. (DEL or SNP ??)
snp
indels
vcf
calls
mpileup
updated 3.8 years ago by
Ram
43k • written 3.8 years ago by
sami
▴ 40
4
votes
30
replies
2.3k
views
Script makes different file then the manual command, but the command is the same
pipeline
linux
awk
3.8 years ago by
stan.aanhane
▴ 30
8
votes
30
replies
5.1k
views
Per Base Sequence Content
sequencing
fastqc
genome
sequence
updated 4.1 years ago by
wm
▴ 560 • written 4.1 years ago by
Researcher
▴ 20
14
votes
30
replies
3.4k
views
Download GTF/GFF annotation data for NT database, not by organism (for STAR alignment)
RNA-Seq
rna-seq
alignment
next-gen
5.0 years ago by
Malka
▴ 80
0
votes
29
replies
3.0k
views
concordance rate between SNP observed and SNP not observed
SNP
updated 5.9 years ago by
Biostar
20 • written 6.0 years ago by
nour.hadjz
▴ 20
7
votes
29
replies
2.4k
views
What's the powerful biological methods for significant genes selection?
gene selection
significant
biological method
R
5.4 years ago by
Chaimaa
▴ 260
11
votes
29
replies
9.8k
views
SummarizedExperiment nrow differs from ncol
DESeq2
SummarizedExperiment
updated 2.5 years ago by
Ram
43k • written 9.9 years ago by
Parham
★ 1.6k
12
votes
28
replies
2.7k
views
Calculating the expression level of genes
Gene expression
RNA-Seq
R
5.9 years ago by
Za
▴ 140
2
votes
28
replies
7.5k
views
Memory use in indexing
Assembly
software error
updated 5.7 years ago by
Ram
43k • written 5.9 years ago by
marongiu.luigi
▴ 710
4
votes
27
replies
4.4k
views
MISO test run fails error of bam file was not found
RNA-Seq
Assembly
software error
next-gen
genome
updated 3.4 years ago by
Biostar
20 • written 7.7 years ago by
JoeDoasi
▴ 10
3
votes
27
replies
5.6k
views
7 follow
Did anyone manage to successfully run HLAScan and get results?
HLA-Typing
NGS
updated 5.7 years ago by
Shiqi Li
• 0 • written 5.9 years ago by
zeynep
▴ 10
9
votes
27
replies
7.6k
views
How do I use Glimmer 3.02?
sequencing
updated 5.3 years ago by
ojelizodun
• 0 • written 6.1 years ago by
nattzy94
▴ 50
3
votes
26
replies
2.0k
views
6 follow
STAR Genome index Error
STAR
updated 6 months ago by
Ram
43k • written 7 months ago by
Prasanna
• 0
1
vote
26
replies
6.3k
views
plink --assoc command gives "NA" for all the analysis (F_A,F_U,A2,CHISQ,P,OR values)
SNP
plink
association
analysis
5.2 years ago by
kushagraprasad24
• 0
10
votes
25
replies
3.5k
views
The interpretation of PCA
DESeq2
R
PCA
updated 5.8 years ago by
Biostar
20 • written 5.8 years ago by
Za
▴ 140
24
votes
25
replies
8.1k
views
How to generate Beta diversity boxplot from phyloseq object?
phyloseq
beta diversity
metagenomics
3.8 years ago by
dpc
▴ 240
8
votes
25
replies
3.0k
views
construction of a database
sql
noSQL
neo4j
database
updated 13 months ago by
Ram
43k • written 3.0 years ago by
Debut
▴ 20
5
votes
24
replies
8.7k
views
Plink1.9 gives error when converting VCF.gz to PED? "Error read failure"
VCF
Plink
5.9 years ago by
DanielC
▴ 170
11
votes
24
replies
5.4k
views
BWA mem skip orientation
assembly
genome
alignment
next-gen
5.0 years ago by
williamsbrian5064
▴ 510
0
votes
24
replies
3.5k
views
converting spaces to tabs in gtf files
NCBI
3.0 years ago by
storm1907
▴ 30
13
votes
24
replies
4.6k
views
Error of bam-to-sam conversion using samtools
samtools
TopHat2
RNA-Seq
BAM
SAM
updated 7.1 years ago by
GenoMax
141k • written 7.1 years ago by
Gary
▴ 480
6
votes
23
replies
2.9k
views
Could you help me set up a VCF filter?
genome
software error
VCF filter
5.8 years ago by
Charlie2
▴ 50
1
vote
23
replies
2.1k
views
FASTQ exctract ID's
fastq
4.4 years ago by
User000
▴ 690
10
votes
23
replies
2.4k
views
Two simillarly annotated sequence has no alignment similarity. Why?
blast
alignment
gene
updated 6.5 years ago by
GenoMax
141k • written 6.5 years ago by
Farbod
★ 3.4k
1
vote
23
replies
1.7k
views
Parse multifasta files based on a values in two columns in a metadata file
parse
mutlifasta
RefSeq
database
strain
3.7 years ago by
jmwhitha
• 0
11
votes
22
replies
4.2k
views
No significant DEG: A request to double check my commands for limma.
limma
differential-gene-expression
updated 21 days ago by
Ram
43k • written 5.0 years ago by
RNAseqer
▴ 260
23
votes
22
replies
49k
views
6 follow
Converting VCF to PLINK .bed binary fileset to check for pedigree errors with KING: How do conversion tools make the PLINK .fam file, without asking …
plink
vcf
fam
gatk
vcftools
updated 17 months ago by
Nicole
• 0 • written 6.0 years ago by
gaelgarcia
▴ 260
1
vote
22
replies
3.6k
views
Genome Index and Alignment- STAR
RNA-Seq
genome
alignment
STAR
updated 5.4 years ago by
Kevin Blighe
87k • written 5.4 years ago by
carolgalah
• 0
3
votes
22
replies
3.2k
views
Making RefSeq in Windows
refseq
7.8 years ago by
Alireza Ebadi Tabrizi
• 0
13
votes
22
replies
11k
views
SVA : Setting up the data from an ExpressionSet and applying sva function to extimate batches
sva
combat
batch-effect
updated 14 days ago by
Ram
43k • written 6.8 years ago by
lessismore
★ 1.3k
4
votes
22
replies
5.6k
views
samtools coverage usage
NGS
updated 2.9 years ago by
Lila M
★ 1.2k • written 2.9 years ago by
smrutimayipanda
▴ 20
5
votes
22
replies
7.5k
views
SVM for classified gene expression data
R
svm
microarray
updated 21 months ago by
Ram
43k • written 8.3 years ago by
Shaurya Jauhari
▴ 50
1
vote
22
replies
6.2k
views
how to use faSplit to split fasta into x files
next-gen
faSplit
sequence
5.5 years ago by
olechnwin
▴ 60
2
votes
22
replies
2.3k
views
6 follow
Find ~1 Mb regions of genome that are shared by two or more WGS samples?
pedigree
linkage
genetics
18 months ago by
Joel Wallenius
▴ 210
5
votes
22
replies
3.3k
views
Differential analysis between two cell lines
R
RNA-Seq
edgeR
differential analysis
5.8 years ago by
Biologist
▴ 290
0
votes
22
replies
3.4k
views
Using java Treeview to analyse genes: how to select and compile group of genes to present?
RNA-Seq
6.1 years ago by
Muha0216
• 0
2
votes
21
replies
4.1k
views
Compute the correlations between submatrices
correlation
R
matrix
foreach
updated 5.1 years ago by
Ram
43k • written 5.1 years ago by
pablo
▴ 300
1
vote
21
replies
3.0k
views
Center and scale RIN values for DESeq2?
deseq2
rin
3.7 years ago by
randalljellis
▴ 90
3
votes
21
replies
3.8k
views
Normalization agilent microarray data?
Microarray
Normalization
updated 3.0 years ago by
Ram
43k • written 4.6 years ago by
mathavanbioinfo
▴ 90
7
votes
21
replies
3.2k
views
Merged.gtf vs. Rnor_6.0.gtf for Raw Counts
RNA-Seq
cuffmerge
raw counts
7.5 years ago by
jmsyl.hong
• 0
0
votes
21
replies
1.1k
views
Expression analysis of LncRNA from RNA-seq data
expression
lncrna
11 weeks ago by
analyst
▴ 30
16
votes
21
replies
2.3k
views
Why there are different hit for a same gene in different species
gene
blast
ortholog
paralog
7.6 years ago by
Farbod
★ 3.4k
6
votes
21
replies
2.8k
views
How to evaluate the similarity of genes between two sample
RNA-Seq
differential
updated 5.6 years ago by
Biostar
20 • written 5.7 years ago by
afli
▴ 190
1,000 results • Page
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Recent Replies
Comment: Telescope issue
by
eleven11
• 0
Thank you! how would I correct that?
Comment: DESeq2 processing problems
by
Ram
43k
This is an open science community, not a place to write an email to Michael Love. Please address the entire community.
Comment: Post-imputation plot
by
kl
▴ 10
All of them are G/C and A/T variants Some seem mismatches based on AF and some seem to need flipping. I guess I am confused, as in the Mich…
Comment: Minimum RAM and Storage requirement for creating PSSM using ncbi-blast-2.2.30+-x
by
Nafi
• 0
Thank you for the reply I have 16 gb of RAM then I can give a try then.
Comment: Alternatives to metanalyisis. P integration. Horiztonal integration RNA-seq
by
i.sudbery
19k
Can you give more details of the data you are planning to use. What is the different studies, what are the comparisons you want to make.
Comment: p-value in CIBERSORT
by
michelafrancesconi9
▴ 20
Thank you so much!!
Comment: POSSUM not working due to incompatible pssm file
by
Ram
43k
Please use the formatting bar (especially the `code` option) to present your post better. You can use backticks for inline code (\`text\` b…
Comment: after selecting specific GO terms to plot, cneplot is not showing any color for
by
Ram
43k
Please use the formatting bar (especially the `code` option) to present your post better. You can use backticks for inline code (\`text\` b…
Comment: Telescope issue
by
GenoMax
141k
Looks like there is a tab missing between the sequence and the quality string.
Comment: Post-imputation plot
by
LChart
3.9k
I would pull out the variants with panel_freq ~ 1 - upload_freq and min(panel_freq, upload_freq) < 0.4. I would check if these are G/C or A…
Comment: Telescope issue
by
eleven11
• 0
Hello the output is A01940:33:GW2208142379th:3:1317:24126:3505 355 chr14 16651125 0 150M = 17034150 122900 GCTAACAGAGTTGAACCTTTCTATTG…
Comment: What does 'bundle_uuid' refer to in this metadata sheet?
by
GenoMax
141k
Looking at the [code here][1] `bundle_uuid` seems to be related to the submissions to HCA. Samples that ran on multiple lanes seem to have …
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by
LauferVA
4.2k
this is very true and increasingly true as time goes forward. thanks for supplementing this post with your own :-)
Comment: What does 'bundle_uuid' refer to in this metadata sheet?
by
jeffrey.maurer.informatics
• 0
Thank you, but I've already checked out that site to see if I was missing anything and I don't see any answers to my questions there.
Answer: p-value in CIBERSORT
by
Aspire
▴ 320
You have not explicitly specified the number of permutations (which are used to calculate the p-value). The default value of the permutatio…
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