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Forum: Extraction of s-farnesyl cysteine, s-palmitoyl cysteine, disulfide , metal binding
uniprot feature the in key
1 hour ago by devhimd • 0
37
votes
30
replies
2.7k
views
7 follow
Forum: Spam In Rss Feeds
Forum
updated 2 days ago by 89k • written 8.7 years ago by 5.2k
3
votes
8
replies
287
views
Forum: Troubleshooting Tips - bcl2fastq creates duplicate reads
bcl2fastq duplicates readgroup demultiplex
6 days ago by DavidStreid • 0
3
votes
1
reply
192
views
Forum: Which bioinformatics tool would you love to see running much faster?
Algorithm optimisation
updated 9 days ago by 33k • written 9 days ago by • 0
2
votes
2
replies
174
views
Forum: Biostars metadata
Biostars metadata
12 days ago by grant.hovhannisyan ★ 2.3k
6
votes
3
replies
326
views
Forum: Differences between SMART-seq2, SMART-seq3, and 10x
cell smartseq UMIs single 10x
updated 15 days ago by ★ 2.3k • written 16 days ago by ▴ 10
0
votes
2
replies
141
views
Forum: How do I use a small font size in questions and answers?
fonts
updated 19 days ago by 89k • written 20 days ago by • 0
0
votes
0
replies
85
views
Forum: Loss of Gene Information: Discrepancies between RNA Sequencing, cDNA Microarray, and qRT-PCR
RNA-Seq microarray qRT-PCR
23 days ago by Antonio R. Franco ★ 4.7k
10
votes
10
replies
2.7k
views
10 follow
Forum: Linearize fasta files
fasta code-golf
updated 27 days ago by ▴ 60 • written 2.6 years ago by 14k
1
vote
1
reply
338
views
Forum: What are some of the problems faced by bioinformaticians because the wet lab personell couldn't understand how data analysis was done?
Problems Bioinformaticians wetlab NGS
updated 28 days ago by ▴ 490 • written 29 days ago by ▴ 10
2
votes
2
replies
251
views
Forum: Internship in research
python genomics
4 weeks ago by vaishnavi ▴ 70
6
votes
2
replies
299
views
Forum: Sequence (annotation) databases in 2021
uniprot annotation sequence ensembl ncbi
updated 4 weeks ago by ★ 1.7k • written 5 weeks ago by ★ 1.5k
17
votes
8
replies
607
views
7 follow
Forum: Where should a biologist start when learning computational biology & systems biology
datascience biology computational bioinformatics systemsbiology
updated 5 weeks ago by ★ 11k • written 5 weeks ago by ▴ 50
8
votes
11
replies
685
views
6 follow
Forum: Paper: Ten simple rules for biologists initiating a collaboration with computer scientists
article opinion Forum
updated 5 weeks ago by 34k • written 11 months ago by 12k
2
votes
1
reply
168
views
Forum: Advise for a computational neophyte
education research thesis master topic
updated 6 weeks ago by 4.0k • written 6 weeks ago by • 0
26
votes
6
replies
3.5k
views
9 follow
Forum: Making a living with bioinformatics
job Forum
updated 6 weeks ago by 77k • written 3.3 years ago by ▴ 150
0
votes
2
replies
249
views
Forum: How to calculate LDDT score for protein structure ?
LDDT PBD
5 weeks ago by aaggencc • 0
13
votes
8
replies
629
views
6 follow
Forum: Why do bioinformatics scientists collaborate and why that's good for science?
Bioinformatics
updated 7 weeks ago by 89k • written 7 weeks ago by ▴ 40
1
vote
3
replies
367
views
Forum: Freelancers for bioinformatics web development
bioinformatics web-development freelance
updated 7 weeks ago by ★ 1.5k • written 7 weeks ago by ▴ 10
9
votes
9
replies
568
views
Forum: Opinions on publishing Research as a Blog?
bioinformatics publishing
8 weeks ago by ponganta ▴ 490
6
votes
4
replies
378
views
Forum: What are the major advances in Bioinformatics since 2016
Bioinformatics Scientist
updated 8 weeks ago by 89k • written 8 weeks ago by ▴ 20
1
vote
0
replies
130
views
Forum: Potential applications of the AlfaFold ?
protein-folding
updated 8 weeks ago by 77k • written 8 weeks ago by ▴ 20
9
votes
3
replies
275
views
Forum: How to manage Biostar email subscriptions?
biostars
updated 9 weeks ago by 20 • written 9 weeks ago by ▴ 490
20
votes
12
replies
32k
views
7 follow
Forum: When To Use Sed Awk Over Perl Or Python
awk perl python Forum
updated 11 weeks ago by ▴ 110 • written 7.7 years ago by 9.0k
8
votes
9
replies
665
views
6 follow
Forum: Choose between AMD and INTEL Server CPU in 2021-2022
hardware server
updated 11 weeks ago by 25k • written 11 weeks ago by ▴ 20
2
votes
1
reply
195
views
Forum: Is there a way to look into funding proposals of published papers?
funding
updated 11 weeks ago by ★ 13k • written 11 weeks ago by ▴ 30
5
votes
6
replies
457
views
Forum: Suggestion of Biostatistics book.
RNA-SEQ R Biostatics
updated 12 weeks ago by ★ 1.5k • written 3 months ago by ▴ 30
7
votes
2
replies
248
views
Forum: Course on genome assembly and annotation
genomics
updated 3 months ago by 34k • written 3 months ago by ▴ 90
115
votes
31
replies
32k
views
24 follow
Forum: Where To Look For Quality Bioinformatics Short Courses And Workshops?
Courses
updated 3 months ago by 34k • written 8.1 years ago by 11k
0
votes
1
reply
243
views
Forum: Opinions on SLAM-Seq for Differential Gene Analysis
DGE RNA-Seq htseq
updated 3 months ago by ★ 2.3k • written 3 months ago by ▴ 30
0
votes
1
reply
142
views
Forum: What can you detect with PE150bp shotgun sequencing with 100X depth
sequencing
updated 3 months ago by ★ 2.5k • written 3 months ago by ▴ 10
1
vote
4
replies
247
views
Forum: Is WGS reliable for larges indels and duplication?
sequencing
updated 3 months ago by ★ 3.1k • written 3 months ago by ▴ 10
0
votes
1
reply
850
views
Forum: Basic beginner bioinformatics for biochemistry for university first year students
books course
updated 3 months ago by 10k • written 3 months ago by • 0
9
votes
7
replies
809
views
6 follow
Forum: When applying to a PhD program in Harvard or MIT, how many publications are recommended?
GradSchool Grad Harvard School Application
updated 5 weeks ago by 34k • written 3 months ago by • 0
0
votes
3
replies
227
views
Forum: what for masters?
microbio masters
updated 3 months ago by ★ 1.5k • written 3 months ago by • 0
0
votes
2
replies
256
views
Forum: Study Materials
learning study materials
3 months ago by AHa • 0
6
votes
3
replies
261
views
Forum: Asking for content suggestions for updated bioinformatics book
python
updated 3 months ago by 54k • written 3 months ago by ▴ 680
9
votes
14
replies
980
views
6 follow
Forum: What are the pain points in genomic variant interpretation/annotation processes?
analysis variant
updated 9 weeks ago by ▴ 430 • written 3 months ago by ▴ 50
3
votes
3
replies
811
views
Forum: Given my background, what are my chances of being accepted into a PhD program at Harvard/MIT?
mit PhD gradschool admission harvard
updated 5 weeks ago by 34k • written 4 months ago by • 0
0
votes
2
replies
305
views
Forum: Bioinformatics internship in Europe
europe internship
updated 4 months ago by • 0 • written 4 months ago by • 0
5
votes
4
replies
350
views
Forum: Detailed Textbooks for bioinformatics and computational biology
biomedical Computational genomics
updated 3 months ago by 34k • written 4 months ago by • 0
10
votes
7
replies
555
views
Forum: Conceptual - Do I need to justify myself if I generate more than three replicates?
statistics conceptual
4 months ago by c_u ▴ 480
2
votes
2
replies
251
views
Forum: reporting differentially expressed genes in a global RNA-seq study
DESeq2 RNA-seq expression heatmaps transcriptomics
4 months ago by thomas.welch ▴ 50
12
votes
8
replies
636
views
Forum: Starting a collective twitter account "Bioinformatician Underhood"
sci-comm
updated 3 months ago by 34k • written 4 months ago by ★ 2.5k
2
votes
2
replies
294
views
Forum: https://biostar.slack.com account
ngs
updated 4 months ago by 106k • written 4 months ago by ▴ 20
1.1k
votes
190
replies
38k
views
104 follow
Forum: What Are The Most Common Stupid Mistakes In Bioinformatics?
software Forum
updated 4 months ago by ▴ 950 • written 10.5 years ago by 20k
6
votes
12
replies
8.4k
views
11 follow
Forum: Online/remote/distance learning/ - partime/fulltime - PhD, Bioinformatics in USA
PhD university Forum
updated 4 months ago by 34k • written 5.0 years ago by ▴ 20
0
votes
2
replies
266
views
Forum: Phylogenomic study: suggestions and tips
evolution species genus phylogenomic phylogenetics
4 months ago by tiagobellintani ▴ 40
0
votes
0
replies
143
views
Forum: How is is is xxx?
sum-of-airs
4 months ago by aa • 0
6
votes
9
replies
598
views
Forum: 2021: state and usuge of compressed file standards better than BAM and FASTQ
fastq compression bam
updated 4 months ago by 9.9k • written 4 months ago by ★ 4.9k
1,000 results • Page 1 of 20
Recent Votes
Answer: Exclude pseudogenes and lncRNA's from DE-analysis?
Comment: Did not take note of my indices (barcodes); is my library salvageable?
Comment: align_and_estimate_abundance error Trinty
Answer: Proper way(s) to perform enrichment analysis in R
Transcriptome assembly -- fastp vs Trimmomatic
A: How to use sed command on multiple files
A: Protein Sequence Alignment From Protein Databank To Cosmic Or Uniprot
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Recent Replies
Comment: align_and_estimate_abundance error Trinty by Princy ▴ 20
yes, it's an error because the output file is empty.
Comment: Did not take note of my indices (barcodes); is my library salvageable? by ATpoint 54k
After all you can also first amplify an aliquot per library (normal amplification PCR), put on gel, excise a precise band and then send tha…
Answer: Exclude pseudogenes and lncRNA's from DE-analysis? by ATpoint 54k
If you are not interested in them then I would exclude them prior to FDR adjustment, that would be the `results` step in `DESeq2` or the `t…
Comment: bcftools by ATpoint 54k
If you really want to compile then download the `tar.bz2` release package from https://github.com/samtools/bcftools/releases which contains…
Comment: align_and_estimate_abundance error Trinty by andres.firrincieli ★ 1.7k
Does not look like an error. With that command line you just prepared the reference. Here the different usage of `align_and_estimate_abunda…
Comment: Bioinformatics tutoring by ATpoint 54k
Very open ended question I am afraid, what did you try? Did you read e.g. the limma user guide which is the standard package for differenti…
Comment: TPM value from DESE2 and significant filterig isssue by ATpoint 54k
If you do not have counts you cannot get TPMs, simple as that. That information is lost in the results table.
Comment: align_and_estimate_abundance error Trinty by Princy ▴ 20
Thanks! I correct my command according to manual **perl /cabinfs/opt/applications/trinity/trinityrnaseq-Trinity-v2.5.1/util/align_and_e…
Comment: align_and_estimate_abundance error Trinty by andres.firrincieli ★ 1.7k
Can you print the usage of `align_and_estimate_abundance.pl` perl /cabinfs/opt/applications/trinity/trinityrnaseq-Trinity-v2.5.1/ut…
Comment: Cell type deconvolution from Bulk RNA-seq data by Anand • 0
Thanks. I'll give it a go.
Answer: TPM value from DESE2 and significant filterig isssue by Nai • 0
My file has values of baseMean log2FoldChange lfcSE stat pvalue padj. I don't have…
Answer: How to number of SNPs in each sample in a multi vcf file by Pierre Lindenbaum 138k
bcftools stats --samples '-' in.vcf.gz
Comment: Makeblastdb error: file does not exist. by Vimalkumar Velayudhan (Vimal) ▴ 270
Is `mydb.fasta` in the same directory, i.e., `C:\Program Files\NCBI\blast-2.12.0+\bin`? If not, you will need to provide the complete pat…
Comment: Use a command on multiple files by Pierre Lindenbaum 138k
what did you try ? https://www.google.com/search?q=loop+multiple+variables+bash
Comment: Invert regex match by cpad0112 17k
in Notepad++, try `^((?!RS=93298723).)*$`. please post example data. Post is not clear with current example data.
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