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1,000 results • Page
6 of 20
Sort: Votes
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Votes
Replies
8
votes
11
replies
2.0k
views
using STAR instead of HISAT2
STAR
updated 22 months ago by
Ram
43k • written 22 months ago by
Chris
▴ 280
7
votes
10
replies
3.8k
views
How to use Ray and BBmap to generate metagenome co-assembly
Ray BBmap assembly
updated 8.0 years ago by
GenoMax
142k • written 8.0 years ago by
thustar
▴ 130
7
votes
4
replies
7.0k
views
Which genome files to use for STAR?
alignment
star
genome
5.0 years ago by
Nico80
▴ 80
7
votes
10
replies
865
views
New insights from old data?
genome
rna-seq
sequence
4.2 years ago by
schlogl
▴ 160
7
votes
5
replies
1.7k
views
RNAseq uneven PCR bias "how to take into best take into account uneven PCR bias ?"
RNA-Seq
rna-seq
updated 6.5 years ago by
Biostar
20 • written 6.6 years ago by
biostar.anon
• 0
7
votes
4
replies
3.6k
views
Abra for indel realignment
realignment
abra
gatk
updated 2.2 years ago by
Ram
43k • written 9.4 years ago by
Danielk
▴ 640
7
votes
8
replies
1.7k
views
fastq dump usage of microRNA-seq data
RNA-Seq
microRNA
fastq
5.0 years ago by
K.patel5
▴ 140
7
votes
1
reply
7.0k
views
createRepeatLandscape in RepeatMasker
RepeatMasker
7.7 years ago by
a.rex
▴ 350
7
votes
8
replies
2.9k
views
Discrepency between number of reads tophat
RNA-Seq
tophat
software-error
updated 19 months ago by
Ram
43k • written 8.7 years ago by
mbio.kyle
▴ 380
7
votes
4
replies
2.0k
views
Transcriptome analysis using tophat and bowtie
tophat
bowtie
BowtieIndex
software error
updated 5.7 years ago by
Ram
43k • written 5.7 years ago by
NAVID.BAVI
• 0
7
votes
5
replies
3.3k
views
Demultiplex fastq files in which barcodes and primers are already removed
RNA-Seq
16S
Demultiplex
7.4 years ago by
kapuriakaran
• 0
7
votes
3
replies
5.5k
views
Ensemble Identifier Of Mirna
mirna
gene-ontology
enrichment
analysis
bioinformatician
microarray
10.5 years ago by
khan
▴ 100
7
votes
9
replies
1.2k
views
Find a gene from RNA-seq data without GTF
RNA-Seq
4.2 years ago by
avino
▴ 20
7
votes
19
replies
3.0k
views
mapping script process sleeping on server.
alignment
updated 2.0 years ago by
Ram
43k • written 9.2 years ago by
lvogel
▴ 30
7
votes
14
replies
1.5k
views
How to Blast with multiple species - Phylogenetic Analysis
ChIP-Seq
alignment
sequence
4.0 years ago by
dimitrischat
▴ 210
7
votes
14
replies
5.0k
views
How to Calulate Allele Frequency from a VCF File?
frequency
allele
VCF
updated 15 months ago by
Jeremy Leipzig
22k • written 15 months ago by
Emoji
▴ 10
7
votes
6
replies
4.6k
views
What's the real difference between these two types of methods for assessing differential expression?
RNA-Seq
differential-expression
methods
updated 2.3 years ago by
Ram
43k • written 9.6 years ago by
Amusement Pork
▴ 60
7
votes
14
replies
2.1k
views
single-cell exome sequencing pipeline
SNP
exom
single-cell
next-gen
7.6 years ago by
zizigolu
★ 4.3k
7
votes
10
replies
3.9k
views
Choosing phylogenetic approaches
phylogeny
updated 2.3 years ago by
Ram
43k • written 9.7 years ago by
Yongjie Zhang
▴ 110
7
votes
9
replies
4.6k
views
Alignment in IGV: extracting specific reads.
iGV
samtools
6.5 years ago by
KVC_bioinfo
▴ 590
7
votes
7
replies
3.1k
views
kmers in rna-seq
RNA-Seq
kmer
updated 2.4 years ago by
Ram
43k • written 9.8 years ago by
sam
▴ 130
7
votes
7
replies
1.4k
views
Machine learning with whole genome sequence data in cancer research?
cancer
genome
5.7 years ago by
dan
▴ 20
7
votes
15
replies
4.3k
views
Biopython Pairwise Alignment output
biopython
needleman
global alignment
4.1 years ago by
tommaso.green
• 0
7
votes
12
replies
3.3k
views
RNA secondary structure - Feature extraction
RNA secondary structure
Structural Fragments
6.0 years ago by
Manu Madhavan
▴ 20
7
votes
4
replies
2.8k
views
Removing a chromosome from a fasta file
fasta
chromosome sequence
6.1 years ago by
t86dan
▴ 30
7
votes
3
replies
2.1k
views
dbSNP vcf file is corrupted?
vcf
gunzip
dbSNP
3.0 years ago by
HB
▴ 30
7
votes
11
replies
1.8k
views
Strandness setting for HISAT2 : BGI sequencing platform
RNA-Seq
Hisat2
updated 3.6 years ago by
GenoMax
142k • written 3.6 years ago by
venura
▴ 70
7
votes
0
replies
2.9k
views
Suffix Array Vs. Compressed Suffix Array Vs. Bwt Search
search
alignment
10.4 years ago by
Rob
6.6k
7
votes
14
replies
4.0k
views
Bowtie2 throws error no. 139
bowtie2
ngs
alignment
software error
sequencing
7.2 years ago by
anu014
▴ 190
7
votes
7
replies
1.5k
views
GSEA analysis
Hi
updated 2.1 years ago by
cvenkat95
▴ 10 • written 2.1 years ago by
sarahawan92
▴ 10
7
votes
4
replies
2.7k
views
RSEM BAM outputs, which one to use for RSeQC?
RSEM
STAR
RSeQC
BAM
2.1 years ago by
Freek
▴ 60
7
votes
3
replies
2.4k
views
Is It Possible To Perform Synteny Study Between A Transcriptome And Genome Of Closely Related Species?
snp
transcriptome
updated 10.3 years ago by
Istvan Albert
100k • written 10.3 years ago by
alok.helix
▴ 120
7
votes
4
replies
923
views
How to extract variants falling in a genomic window from a VCF?
vcf
updated 2.9 years ago by
GenoMax
142k • written 2.9 years ago by
francois
▴ 80
7
votes
16
replies
21k
views
featureCounts: Low percentage of assigned fragments
RNA-Seq
rna-seq
7.8 years ago by
aggregatibacter
▴ 180
7
votes
9
replies
2.3k
views
statistical analysis of RNA-seq
RNA-Seq
Statistics
7.6 years ago by
statfa
▴ 760
7
votes
2
replies
5.3k
views
Store Structural Variants Into Vcf
vcf
11.2 years ago by
Tomáš Beluský
▴ 90
7
votes
7
replies
1.8k
views
Nextflow rnaseq finishing early
RNA-seq
nextflow
updated 13 months ago by
Ram
43k • written 13 months ago by
Raygozak
★ 1.4k
7
votes
14
replies
6.0k
views
transcriptome index for Kalisto
RNA-Seq
updated 7.3 years ago by
Biostar
20 • written 7.5 years ago by
1769mkc
★ 1.2k
7
votes
18
replies
8.6k
views
9 follow
Cuffmerge running error
RNA-Seq
updated 2.3 years ago by
Ram
43k • written 9.5 years ago by
hana
▴ 190
7
votes
4
replies
2.2k
views
Error: missing values and NaN's not allowed if 'na.rm' is FALSE
Genome
Software
22 months ago by
sunnykevin97
▴ 980
7
votes
13
replies
4.5k
views
6 follow
Is trimming necessary for RNAseq?
RNA-Seq
trimming
fastqc
updated 6 months ago by
ATpoint
82k • written 3.5 years ago by
Pac314
▴ 10
7
votes
17
replies
6.6k
views
HeatMap: how to cluster only the rows and keep order of the heatmap's column labels as same as in the df?
R
gene
genome
5.9 years ago by
WUSCHEL
▴ 760
7
votes
29
replies
2.5k
views
What's the powerful biological methods for significant genes selection?
gene selection
significant
biological method
R
5.5 years ago by
Chaimaa
▴ 260
7
votes
2
replies
2.4k
views
Trying to reproduce mirbase results locally with BLAST
blast
6.5 years ago by
avino
▴ 20
7
votes
5
replies
5.6k
views
How to know if it is 'one-color' or 'two-color' microarray?
microarray
RNA-Seq
ChIP-Seq
sequencing
7.6 years ago by
learnBioinformatics
▴ 60
7
votes
7
replies
1.5k
views
Am I unnecessarily 'missing' a high number of true-positive DE genes? Or do I just not understand the statistics?
RNA-Seq
R
edgeR
updated 3.3 years ago by
Ram
43k • written 3.3 years ago by
bkleiboeker
▴ 370
7
votes
9
replies
6.6k
views
Converting Gene Predictions In Glimmer To Protein Sequences.
script
biopython
updated 2.3 years ago by
Ram
43k • written 10.8 years ago by
genomelover
▴ 50
7
votes
5
replies
4.0k
views
Trinity de novo transcript assembly too many transcripts
RNA-Seq
Assembly
updated 21 months ago by
Ram
43k • written 8.9 years ago by
Mehmet
▴ 820
7
votes
3
replies
1.6k
views
What are the best tools for Genome assembly
Assembly
genome
updated 11 months ago by
Ram
43k • written 5.8 years ago by
Shawrob400
▴ 10
7
votes
12
replies
2.6k
views
Is this variant probably an artifact?
VCF
Exome Sequencing
6.2 years ago by
Sharon
▴ 610
1,000 results • Page
6 of 20
Recent Votes
constructing pangenome through psvcp
Differential Expression with limma: Contrast and Design Matrix, combinatorial approach valid?
Differential Expression with limma: Contrast and Design Matrix, combinatorial approach valid?
Answer: RNAseq coverage vs depth for transcript isoform expression?
Answer: ChatGPT optimized for bioinformatics questions
Comment: ChatGPT optimized for bioinformatics questions
C: How can I perform Differential expression analysis with just one control and one
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Comment: constructing pangenome through psvcp
by
analyst
▴ 50
Fixed the issue by updating nucmer version.
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
GenoMax
142k
Can you create a small test database (`makeblastdb`) from your query file and try to run the search against the same file and see if that w…
Comment: calculating genomic coverage/ base overlap in R
by
1769mkc
★ 1.2k
you can `dput(head(df)` for the both the data frame so that other can test for possible solutions
Comment: Broad and Narrow peaks
by
ATpoint
82k
What is your question? It's unclear.
Comment: Help with Biopython for Beginner
by
Joe
21k
One question before offering more solutions: are you trying to learn Python with this as an example problem, or are you trying to find the …
Answer: RNAseq coverage vs depth for transcript isoform expression?
by
Gordon Smyth
★ 7.2k
See the exploration of isoform precision vs read length and sequencing depth in our paper: https://doi.org/10.1093/nar/gkad1167. Table 1 o…
Comment: How to handle duplicated genes in TCGA data?
by
txema.heredia
▴ 130
Why does the tool require gene symbols and not ensembl ids? Is it retrieving information from somewhere else? If so, your best bet is to fi…
Comment: how to treat the replicates while performing WGCNA
by
mavy
▴ 10
Thank you so much for your response . Really appreciate it. I will try the way you mentioned and will get back if I have any query
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
hophuquy0944
• 0
i have the same error, i used high performance computer, here is my code to set memory. srun --nodes=1 --ntasks=1 --cpus-per-task=24 -…
Comment: Overlapping Ranges within Granges object
by
Alex Reynolds
35k
Yes, `bedmap` will perform operations on one or two BED files.
Comment: B allele frequency (BAF)
by
aidangcruickshank
▴ 10
Apologies for resurrecting this post, but any idea what that functions "regions" paramater takes? Would it be a GRanges object? The documen…
Comment: How to handle duplicated genes in TCGA data?
by
Ngrin
• 0
Thanks @txema.heredia.The tool I am going to use only accepts gene symbols. This is the reason. So I cannot use ENS IDs.
Answer: Design for complex RNA-Seq experiment using Deseq2
by
swbarnes2
14k
This is a rather small experiment, I don't know that you can establish a meaningful baseline for each patient with a single pre sample, and…
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4.2k
@bk11 i changed this comment to an answer. if you disagree please let me know. thanks for sharing your knowledge. VAL
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by
hannes.bongartz
• 0
Thanks for the input. Unfortunately, that didn't help either. I appreciate any other tips. Thank you
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