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1,000 results • Page
3 of 20
Sort: replies
Rank
Views
Votes
Replies
7
votes
18
replies
4.2k
views
Bowtie indexing of a fasta file that consists of a large amount of sequences
bowtie
alignment
genome
6.9 years ago by
valerie
▴ 100
1
vote
18
replies
1.4k
views
Find tissues that are functionally related
tissue
functionally-related
gene-expression
updated 4.1 years ago by
Biostar
20 • written 4.1 years ago by
Natasha
▴ 40
20
votes
17
replies
5.5k
views
HiSeq 4000 PhiX screening and removal using bbduk
spikein
filtering
bbmap
bbduk
phix
6.1 years ago by
Anand Rao
▴ 560
27
votes
17
replies
19k
views
12 follow
Dotplot for filtered pathways result
pathways
ggplot2
clusterProfiler
r
dotplot
21 months ago by
Leite
★ 1.2k
1
vote
17
replies
3.6k
views
Error executing bedops convert2bed
bedops
bed
vcf
convert2bed
6.7 years ago by
lakhujanivijay
5.8k
6
votes
17
replies
2.1k
views
6 follow
Issue with reverting bam file back to fastq files
next-gen
assembly
genome
alignment
5.3 years ago by
williamsbrian5064
▴ 480
2
votes
17
replies
4.0k
views
Problem With Sam To Bam Converison After Alignment Of Scaffolds To Reference
sam
bam
alignment
samtools
updated 9.7 years ago by
Devon Ryan
103k • written 9.7 years ago by
Rohit
★ 1.5k
0
votes
17
replies
3.3k
views
WGCNA error: "Error in cutreeDynamic"
rna-seq
wgcna
software error
R
next-gen
updated 21 months ago by
Dio
• 0 • written 5.0 years ago by
giuseppe0525
▴ 20
0
votes
17
replies
2.7k
views
I am not able to let R read my file
R
fasta
updated 2.5 years ago by
Arup Ghosh
3.2k • written 2.5 years ago by
trejomarco6
• 0
0
votes
17
replies
4.4k
views
Finding True SNPs after hard filtering on GATK
SNP
updated 12 months ago by
Ram
40k • written 8.0 years ago by
jigarnt
▴ 30
5
votes
17
replies
3.9k
views
Interpreting Genome_Structural_Correction Block_Bootstrap.Py
statistics
encode
9.6 years ago by
14134125465346445
★ 3.6k
1
vote
17
replies
5.3k
views
BBMap: Mapping Fails
BBTools
BBMap
Mapping
updated 7.5 years ago by
GenoMax
134k • written 7.5 years ago by
cacampbell
▴ 50
4
votes
17
replies
2.4k
views
Cufflinks analysis and gffread
RNA-Seq
Assembly
updated 5.9 years ago by
Kevin Blighe
86k • written 5.9 years ago by
qudrat
▴ 100
16
votes
17
replies
2.9k
views
6 follow
RNA seq pipeline
RNA-Seq
5.9 years ago by
dimitrischat
▴ 200
0
votes
17
replies
1.2k
views
Transcripts with no read support
RNA-Seq
alignment
assembly
salmon
3.0 years ago by
Dunois
★ 2.4k
12
votes
17
replies
4.2k
views
How do I generate all possible Newick Tree permutations for a set of species given an outgroup in Python?
Python
Newick
phylogenetics
tree
phylogeny
6.0 years ago by
anonymous1192976466
▴ 50
3
votes
17
replies
1.2k
views
copy number became inaccurate when downsampling different number fastq reads from a whole fastq file
CNV
DownSample
fastq
copy number not accurate
3.6 years ago by
lffu_0032
▴ 90
7
votes
17
replies
3.4k
views
How to find the differences in aligned bam files
RNA-Seq
6.6 years ago by
1769mkc
★ 1.1k
3
votes
17
replies
2.5k
views
FAIRE for non-standard cultivar, mapping to reference, MAPQ
sequencing
faire
mapping
2.5 years ago by
boczniak767
▴ 840
0
votes
17
replies
2.4k
views
Fail running blastdb and blastn on my own computer
blast
5.9 years ago by
annette440
• 0
2
votes
17
replies
2.3k
views
Snakemake Megahit error
megahit
Snakemake
22 months ago by
blackadder
▴ 30
2
votes
17
replies
4.7k
views
Coding Potential Calculator (CPC) error
RNA-Seq
updated 5.5 years ago by
h.mon
34k • written 5.5 years ago by
Janey
▴ 30
2
votes
17
replies
7.8k
views
7 follow
EXITING: because of fatal INPUT file error: could not open read file
mapping
genome
fastq
STAR
updated 18 months ago by
todd.ugine
• 0 • written 2.7 years ago by
merfer0206
• 0
8
votes
17
replies
3.6k
views
Getting nan value in vcftool relatedness command?
vcftools
relatedness
5.6 years ago by
Sharon
▴ 590
4
votes
17
replies
2.4k
views
Any strategy to find out the reason of having unusual Coefficient of Variation (CV) for gene expression data?
microarray
gene-expression
CV
error
4.2 years ago by
Jurat Shahidin
▴ 100
20
votes
17
replies
2.4k
views
How to add "transcript" feature to a gtf file?
gtf
updated 11 weeks ago by
1769mkc
★ 1.1k • written 2.6 years ago by
pomodoro_sinensis
▴ 110
3
votes
17
replies
6.7k
views
Removing fastq duplicates
sequence
next-gen
ChIP-Seq
2.8 years ago by
C4
▴ 10
7
votes
17
replies
3.9k
views
Strange MA-plot using DESeq2
rna-seq
R
deseq2
updated 6.7 years ago by
Biostar
20 • written 6.8 years ago by
stan
▴ 80
9
votes
17
replies
1.9k
views
Creating intergenic bam file
R
genome
intergenic
alignment
7.1 years ago by
erincyurtman
• 0
7
votes
17
replies
6.2k
views
HeatMap: how to cluster only the rows and keep order of the heatmap's column labels as same as in the df?
R
gene
genome
5.2 years ago by
WUSCHEL
▴ 720
1
vote
17
replies
2.1k
views
Normalizing BLAST results
blast
updated 6.0 years ago by
Biostar
20 • written 6.1 years ago by
db
• 0
7
votes
16
replies
3.2k
views
How do I get a GFF file
snp
4.3 years ago by
apl00028
▴ 80
0
votes
16
replies
4.2k
views
Issue using MaSuRCA-3.2.6
Assembly
assembler
updated 5.1 years ago by
Biostar
20 • written 5.3 years ago by
Chvatil
▴ 120
3
votes
16
replies
1.1k
views
How to bring all reads to the same length? Any tool?
Read-length
Bed
updated 3 months ago by
Ram
40k • written 3.7 years ago by
Ankit
▴ 390
3
votes
16
replies
11k
views
combining z-scores into a single z-score value
excel
R
statistics
updated 3 months ago by
Ram
40k • written 4.5 years ago by
Star
▴ 60
3
votes
16
replies
3.5k
views
GC content of 10x Genomics
fastqc
10x
GC content
updated 3.1 years ago by
Eugene A
▴ 170 • written 4.5 years ago by
Assa Yeroslaviz
★ 1.8k
0
votes
16
replies
4.8k
views
Unable To Delete Tmp Files Of Samtools Sort
samtools
sort
10.3 years ago by
chentong.biology
▴ 50
1
vote
16
replies
6.1k
views
After sorting bam file not able to index ?
alignment
next-gen
updated 4.6 years ago by
Biostar
20 • written 4.7 years ago by
sunnykevin97
▴ 970
0
votes
16
replies
3.4k
views
Gff to genbank - feature is missing
DNA
augustus
gff3
genbank
updated 4 months ago by
Ram
40k • written 5.3 years ago by
rororo
• 0
9
votes
16
replies
1.5k
views
feature extraction from SNPs in R
R
gene
sequence
SNP
4.9 years ago by
bioinfo456
▴ 150
7
votes
16
replies
3.2k
views
cutting overrepresented sequences (recognized by fatsqc)
sequencing
genome-sequence
fastqc
genome
sequence
3.5 years ago by
Researcher
▴ 20
0
votes
16
replies
3.9k
views
Count read with summarizeOverlaps result 0 for all sample
RNA-Seq
updated 14 months ago by
Ram
40k • written 8.4 years ago by
bharata1803
▴ 550
2
votes
16
replies
1.6k
views
I want to know is it a true method ?
vcf
SNP
realignment
5.5 years ago by
Siavash Salek Ardestani
▴ 20
9
votes
16
replies
3.3k
views
6 follow
covert SAM to full length fasta
fasta
SAM
updated 6 months ago by
Ram
40k • written 5.1 years ago by
marongiu.luigi
▴ 690
16
votes
16
replies
2.7k
views
Using DESeq2 results for building a classifier
deseq2
RNA-Seq
differentially expressed genes
5.5 years ago by
bioinfo456
▴ 150
9
votes
16
replies
1.1k
views
I am trying to select some reference genome region of a bam file, but got an error
SAMTOOLS
updated 3.1 years ago by
lieven.sterck
14k • written 3.1 years ago by
schlogl
▴ 150
0
votes
16
replies
2.1k
views
How to convert ncbi gff file to ensembl gff format
bcftools
gff
updated 6 months ago by
Ram
40k • written 13 months ago by
yoser4
▴ 10
13
votes
16
replies
5.4k
views
Mask or trim primer sequences in Amplicon sequencing
amplicon
mask
trimming
fastq
6.7 years ago by
Paul
★ 1.5k
5
votes
16
replies
5.5k
views
Remove duplicate lines based on specific columns
r
python
intersect
reads
updated 5.0 years ago by
Ram
40k • written 5.0 years ago by
dzisis1986
▴ 60
4
votes
16
replies
1.8k
views
How to extract intron counts from total RNA Sequencing?
featurecounts
RNAseq
htseq
STAR
10 months ago by
barrypraveen
▴ 110
1,000 results • Page
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Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
Comment: Error in bowtie2
Recommended Tools For Alternative Splicing Detection From Rna-Seq Data
Comment: Using STAR aligner to build index of hg38
Answer: How to sort using samtools
The Biostar Handbook. A bioinformatics e-book for beginners.
The Biostar Handbook. A bioinformatics e-book for beginners.
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Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I tried the commands you recommended in the bash/command line. When I tried `find "${bam_directory}" -type f -name "*.bam" | xargs samtools…
Comment: Hugo_Symbol to Entrez ID
by
GenoMax
134k
Can you provide some examples of HUGO ID's you are unable to convert?
Comment: Using STAR aligner to build index of hg38
by
GenoMax
134k
GTF file includes gene models/annotation information. You could also use a program like `salmon/kallisto` with just human transcriptome to…
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Pierre Lindenbaum
157k
then test each files. find "${bam_directory}" -type f -name "*.bam" | xargs samtools quickcheck file "${reference_gen…
Answer: Ties in reranked list
by
alserg
▴ 840
The problem here is not the ties, but that your gene IDs in the pathway list (ensemble human genes) does not match the names of the stats v…
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I did not use the space between the `-u` flag and the `-o` flag (the blank line) but I did do what you recommended: # Run CuffDiff wit…
Answer: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Pierre Lindenbaum
157k
how about simply trying: ``` cuffdiff -u "${annotation_gtf}" \ -o "${output_directory}" \ -b "${reference_genome}" \ -p "${num_th…
Comment: Using STAR aligner to build index of hg38
by
Grace
• 0
Yes, I know also need a GTF file. So the GTF file will include RNA information? Thanks for your answer!!
Comment: STAR Intron Motif Script Gives Segmentation fault Error
by
Y
• 0
I will try and figure it out on my own given what you all have mentioned. Thank you for your time.
Comment: Using STAR aligner to build index of hg38
by
Amitm
★ 2.2k
Have you gone through the manual [here][1]. In addition to the genome fasta file, you would also need a GTF file (of gene annotations) to b…
Comment: Highly inflated p-values in GWAS by regenie
by
4galaxy77
2.8k
You still definitely need to include principle components as covariates, even if your data is from the same ethnicity. Not including those …
Answer: How to create structural variants ground truth for alignment of two long-read ge
by
Christophe
• 0
Hi, D-genies is using minimap2 to align both genomes and minimap2 is chaining local alignments to produce a global one. If the SV are sm…
Comment: Ties in reranked list
by
Hamza
• 0
In this case there are no results in my fgsea object unfortunately
Comment: Genes with promoter and enhancer regions as GTF
by
abis.1819104
• 0
how to generate promoters.bed and enhancer.bed files from gtf file
Comment: Deepvariant variant calling by singularity
by
jeffhsu3
• 0
I am having a similar issue was this ever resolved? Getting this error: I0922 22:22:08.794076 140356687390528 make_examples_core.py:257] O…
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