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460 results • Page
2 of 10
Sort: Views
Rank
Views
Votes
Replies
11
votes
13
replies
4.5k
views
6 follow
Forum:
NGS Data storage solutions for small organisations or big labs
big-data
HTS
storage
NGS
metagenomics
updated 11 months ago by
Ram
43k • written 4.9 years ago by
bioinfo
▴ 830
18
votes
7
replies
4.5k
views
Forum:
Review of the CIGAR string format
alignment
updated 11 months ago by
Ram
43k • written 5.5 years ago by
Juke34
8.6k
26
votes
10
replies
4.4k
views
6 follow
Forum:
Why not merging?
fastq
sequencing
updated 11 months ago by
Ram
43k • written 6.8 years ago by
finswimmer
16k
47
votes
30
replies
4.3k
views
10 follow
Forum:
Bioinformatics software distribution
software
updated 11 months ago by
Ram
43k • written 7.2 years ago by
Petr Ponomarenko
★ 2.8k
15
votes
6
replies
4.3k
views
Forum:
Which database software is better for bioinformatics web development? MySQL or MongoDB?
database
mysql
mongodb
software
updated 11 months ago by
Ram
43k • written 5.6 years ago by
mcclintock
▴ 10
73
votes
14
replies
4.3k
views
12 follow
Forum:
Things you wish you knew when entering the bioinformatics field
pitfalls
advice
updated 10 months ago by
Ram
43k • written 3.6 years ago by
jared.andrews07
★ 16k
10
votes
11
replies
4.2k
views
7 follow
Forum:
What made you interested in Bioinformatics?
career
updated 11 months ago by
Ram
43k • written 6.4 years ago by
Hussain Ather
▴ 990
33
votes
23
replies
4.2k
views
8 follow
Forum:
Is there a file format better suited for the era of pangenomics than the .vcf? What are its attributes?
VCF
file-format
genomics
updated 10 months ago by
Jeremy Leipzig
22k • written 11 months ago by
LauferVA
4.2k
40
votes
23
replies
4.2k
views
12 follow
Forum:
Why learn programming in bioinformatics?
Programming
updated 11 months ago by
Ram
43k • written 5.7 years ago by
joselu
▴ 110
15
votes
13
replies
4.2k
views
8 follow
Forum:
Does a PhD or work experience set you up better to do bioinformatics work in industry?
advice
industry
phd
updated 3 months ago by
benformatics
3.9k • written 18 months ago by
buhbs
▴ 30
36
votes
39
replies
4.1k
views
11 follow
Forum:
Survey/Vote: If you could double the speed of any three commandline tools, which three would they be?
blast
RNA-Seq
alignment
next-gen-sequencing
updated 11 months ago by
Ram
43k • written 6.5 years ago by
dhbradshaw
▴ 130
56
votes
19
replies
4.1k
views
9 follow
Forum:
How to apply for a job
resume
cover-letter
application
CV
updated 11 months ago by
Ram
43k • written 6.8 years ago by
Emily
23k
37
votes
16
replies
4.0k
views
9 follow
Forum:
Sanger sequencing is no longer the gold standard?
Sanger
NGS
updated 11 months ago by
Ram
43k • written 6.5 years ago by
lamteva.vera
▴ 220
6
votes
8
replies
4.0k
views
6 follow
Forum:
Workstation : More RAM or Faster RAM
RAM
Configuration
workstation
updated 11 months ago by
Ram
43k • written 5.6 years ago by
venuraherath
▴ 20
0
votes
3
replies
3.9k
views
10 follow
Forum:
Discussion question: Cutoff for SNP phylogeny
Phylogenetics
SNP
written 17 months ago by
SushiRoll
▴ 120
25
votes
11
replies
3.7k
views
6 follow
Forum:
Site Use Guidelines
meta
biostars
Guidelines
updated 11 days ago by
Ram
43k • written 14.6 years ago by
Istvan Albert
100k
30
votes
13
replies
3.7k
views
11 follow
Forum:
what is your daily must see bioinformatics web site?
learn
technology
updated 10 months ago by
Ram
43k • written 9.6 years ago by
Medhat
9.7k
19
votes
9
replies
3.7k
views
Forum:
How to self-study (get onto) Bioinformatics and Data Analysis for Genomic, for working with real life genomics data?
R
genome
RNA-Seq
Python
updated 11 months ago by
Ram
43k • written 5.8 years ago by
WUSCHEL
▴ 750
8
votes
9
replies
3.7k
views
8 follow
Forum:
How can I contribute to the Bioinformatics community as a Software Engineer
software-engineer
updated 11 months ago by
Ram
43k • written 5.5 years ago by
Chen Sun
★ 1.1k
0
votes
3
replies
3.6k
views
Forum:
How to know that our trinity denovo assembly is good or not?
Assembly
updated 11 months ago by
Ram
43k • written 5.4 years ago by
ashokkumar.mb
• 0
21
votes
12
replies
3.6k
views
Forum:
How to Use Biostars Part-3: Formatting Text and Using GitHub Gists
meta
how-to
documentation
3 months ago by
Ram
43k
4
votes
0
replies
3.5k
views
Forum:
How can unique molecular identifiers (UMIs) help to reduce quantitative biases?
NGS
Variant-Calling
Unique-Molecular-Identifier
updated 11 months ago by
Ram
43k • written 4.9 years ago by
David Langenberger
11k
3
votes
8
replies
3.5k
views
6 follow
Forum:
SSD or HHD for genome analysis
hardware
genome
sequencing
updated 11 months ago by
Ram
43k • written 4.6 years ago by
Neil
▴ 20
19
votes
6
replies
3.5k
views
Forum:
ngs vs microarray
microarray
ngs
updated 11 months ago by
Ram
43k • written 5.8 years ago by
au.rinki.bio
▴ 20
6
votes
8
replies
3.4k
views
Forum:
A hot topic in Bioinformatics 2017
paper
updated 11 months ago by
Ram
43k • written 7.1 years ago by
XBria
▴ 90
22
votes
11
replies
3.4k
views
Forum:
ExpressionSet in the age of tidyverse
expressionset
R
bioconductor
updated 11 months ago by
Ram
43k • written 6.0 years ago by
igor
13k
17
votes
14
replies
3.3k
views
Forum:
Asking for feedback on a Python library for computing alignments
alignment
Python
updated 11 months ago by
Alexander
▴ 220 • written 2.3 years ago by
bernhard.liebl
▴ 60
29
votes
18
replies
3.3k
views
7 follow
Forum:
Should I quit PhD program?
education
career
research
phd
updated 11 months ago by
Ram
43k • written 6.0 years ago by
George
▴ 30
9
votes
11
replies
3.3k
views
7 follow
Forum:
Fields with biggest demand for bioinformatics?
career
updated 11 months ago by
Ram
43k • written 5.3 years ago by
BioinformaticsLad
▴ 200
0
votes
2
replies
3.2k
views
Forum:
I’m considering a career transition from software engineering to bioinformatics. Is it a good fit for me?
career
updated 4 months ago by
GenoMax
142k • written 4 months ago by
Mango
▴ 10
13
votes
18
replies
3.2k
views
6 follow
Forum:
Bioinformatics independent study group
Proteomic
RNA-Seq
Genomic
updated 11 months ago by
Ram
43k • written 6.7 years ago by
Leite
★ 1.3k
38
votes
17
replies
3.2k
views
7 follow
Forum:
Why does bioinformatics use a special format like a BAM file instead of a database like SQLite?
sam
bam
samtools
updated 10 months ago by
Ram
43k • written 3.7 years ago by
kojix2
▴ 250
18
votes
2
replies
3.2k
views
Forum:
Scripts on the Web: Bioinformatics Recipes
bioinformatics
updated 11 months ago by
Ram
43k • written 6.1 years ago by
Istvan Albert
100k
9
votes
3
replies
3.2k
views
Forum:
Why doesn't Swift have an active bioinformatics community project like rust-bio, Biogo and BioJulia?
Swift
updated 11 months ago by
Ram
43k • written 6.3 years ago by
stevin
• 0
29
votes
10
replies
3.2k
views
Forum:
How do you perform functional testing for your Bioinformatics tools?
development
testing
bug
reliability
updated 12 months ago by
Ram
43k • written 7.2 years ago by
shenwei356
8.5k
7
votes
6
replies
3.1k
views
Forum:
transition to bioinformatics
career
wet-lab
updated 11 months ago by
Ram
43k • written 5.9 years ago by
ejms
• 0
2
votes
6
replies
3.1k
views
Forum:
RIP CentOS, CentOS 8 to be EOL'd in 2021
centos
linux-distro
os
updated 10 months ago by
Ram
43k • written 3.4 years ago by
5heikki
11k
10
votes
8
replies
3.1k
views
6 follow
Forum:
Cancer research for computer science student
cancer
research
computer-science
updated 6 months ago by
SANDHYA
• 0 • written 6.5 years ago by
mhasa006
▴ 70
64
votes
24
replies
3.0k
views
12 follow
Forum:
How much I must feel useless?
machine-learning
Programming
statistics
updated 11 months ago by
Ram
43k • written 5.6 years ago by
Molecular_genetics
▴ 80
4
votes
6
replies
3.0k
views
Forum:
Looking for advice on making a grad school decision on bioinformatics/computational biology programs
computational-biology
graduate-program
updated 11 months ago by
Ram
43k • written 7.1 years ago by
JQX
▴ 10
3
votes
4
replies
3.0k
views
6 follow
Forum:
Links for forums or discussion groups about machine learning, aging, genomics and artificial intelligence?
genome
updated 11 months ago by
Ram
43k • written 6.2 years ago by
tfhahn
▴ 50
12
votes
12
replies
3.0k
views
Forum:
Programming interview via Skype
programming-test
interview
updated 11 months ago by
Ram
43k • written 5.9 years ago by
padgaus
▴ 50
0
votes
1
reply
3.0k
views
Forum:
Bioinformatics Master thesis
thesis
updated 11 months ago by
Ram
43k • written 5.3 years ago by
lucas_
▴ 20
12
votes
2
replies
3.0k
views
Forum:
List of genome annotation tools (>140)
gene
genome
updated 11 months ago by
Ram
43k • written 4.7 years ago by
Juke34
8.6k
34
votes
22
replies
2.9k
views
11 follow
Forum:
Have we failed as bioinformatician in this time of COVID-19
Bioinformatician
COVID-19
updated 11 months ago by
Ram
43k • written 4.0 years ago by
heididunst
▴ 10
4
votes
6
replies
2.9k
views
Forum:
NGS Data Storage Best Practices (Clinical)
NGS
clinical
storage
updated 11 months ago by
Ram
43k • written 4.4 years ago by
Robert Sicko
▴ 630
45
votes
19
replies
2.9k
views
9 follow
Forum:
Where and how NGS techniques are heading for the next 5 years?
next-gen
NGS
updated 11 months ago by
Ram
43k • written 6.9 years ago by
lakhujanivijay
5.8k
3
votes
7
replies
2.9k
views
Forum:
Strategies for group submisions to SRA (BioProject)
NCBI
SRA
next-gen
Archive
Core-Facility
updated 11 months ago by
Ram
43k • written 5.5 years ago by
A. Domingues
★ 2.7k
8
votes
6
replies
2.8k
views
Forum:
Demonstrating Exponential Growth In Pre-School
education
written 10.2 years ago by
Asaf
10k
36
votes
34
replies
2.8k
views
9 follow
Forum:
New to bioinformatics.....
gene
updated 11 months ago by
Ram
43k • written 6.1 years ago by
sms.00196
• 0
460 results • Page
2 of 10
Recent Votes
Answer: GRCh38.gmap file
Comment: GRCh38.gmap file
Answer: GRCh38.gmap file
Answer: GRCh38.gmap file
Answer: Design matrix Differential expression analysis
A: PCA plot from read count matrix from RNA-Seq
A: PCA plot from read count matrix from RNA-Seq
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Comment: The GDC Legacy Archive is retiring soon.
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If you are talking about methylation idat files, they were all migrated from legacy to the data portal. If you are talking about other pl…
Comment: How to access TCGA samples that were treated with a specific drug?
by
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These TCGA samples were taken 10 ~ 20 years ago, and there is no way they have been treated by the modern immunotherapy. Are you sure tha…
Comment: Design matrix Differential expression analysis
by
Gordon Smyth
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> When is it recommended to use the design <- cbind(Intercept=1,Group=c(-1,-1,-1,1,1,1)) It is not recommended. No such code appears anywh…
Comment: GRCh38.gmap file
by
lorena9132
▴ 10
Hi Mathew, The .txt files provided in the link differ from the .gmap files, but after several attempts, I succeeded in generating the .gma…
Answer: GRCh38.gmap file
by
Mathew
▴ 30
Hi, I have spent some time searching for a GRCH38 map file for you. I have found a certain publication that uses a hg38 genetic map file…
Answer: Association study using SNP data
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Mathew
▴ 30
Hi, You asked three questions so I will attempt to help the best I can **What should be the optimal GC score cutoff** It sounds li…
Comment: Design matrix Differential expression analysis
by
SHN
▴ 40
Thank you for your explanation. I am working on the cell lines and I am investigating the effect of the treatment on the expression level, …
Answer: Issues with Mixture file when using CIBERSORTx
by
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• 0
I ran into same issue of "duplicated gene symbol(s)": 1: In CIBERSORTxFractions(sigmatrix = sigmatrix, mixture = mixture, : 22292 dup…
Comment: creating batch colum for batch correction
by
Expert
▴ 10
Thank you for your response. These samples were collected from the same patient at different time points(day 0, 8 and 18) and these are ind…
Comment: creating batch colum for batch correction
by
ATpoint
82k
Not sure I follow. Can you first of all describe which comparisons you want to make, pretending there was no batch effect at all. Write dow…
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by
Ruqaiya
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This the tool still problematic?
Comment: Segmentation error while using a tool
by
Ruqaiya
• 0
Thank you for the advice, i'll keep that in mind.
Comment: Segmentation error while using a tool
by
Ruqaiya
• 0
I am trying to reproduce some bioinformatics data from a research paper-[this paper][1] as i was asked to work on an MRSA bioinfo project, …
Answer: Segmentation error while using a tool
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Mensur Dlakic
★ 27k
Most likely you didn't do anything wrong. Going by your previous post that you have only 8 GB of RAM, the most likely reason is the lack of…
Comment: Segmentation error while using a tool
by
Thanujay S
• 0
Hey @Ruqaiya! Can you please give more details on why smalt is opted here? According to what I can see, the tool has become obsolete! Why …
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