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116,622 results • Page
1 of 2333
Sort: Views
Rank
Views
Votes
Replies
65
votes
3
replies
295k
views
Understanding up and down regulated genes from LOG2 foldchange or foldchange
protein
gene-expression
RNA-Seq
updated 13 months ago by
Ram
43k • written 9.9 years ago by
Whoknows
▴ 960
226
votes
39
replies
288k
views
33 follow
Gene Id Conversion Tool
david
updated 6 months ago by
Ram
43k • written 14.5 years ago by
Renee
▴ 620
279
votes
29
replies
284k
views
25 follow
How To Filter Mapped Reads With Samtools
samtools
updated 17 months ago by
Ram
43k • written 11.5 years ago by
sohadb1357
▴ 640
332
votes
92
replies
239k
views
58 follow
Tools To Calculate Average Coverage For A Bam File?
coverage
bam
sequencing
updated 8 months ago by
kalavattam
▴ 190 • written 13.3 years ago by
Biomed
5.0k
43
votes
24
replies
205k
views
25 follow
How To Update R In Ubuntu ?
r
ubuntu
updated 2.1 years ago by
matthew.pace
▴ 60 • written 10.4 years ago by
Arpitha Hebbar
▴ 210
12
votes
6
replies
200k
views
How can I uninstall my packages in Rstudio
R
Bioconductor
updated 3.6 years ago by
zx8754
11k • written 6.2 years ago by
hassanlou110
▴ 40
120
votes
31
replies
199k
views
17 follow
Correct Way To Parse A Fasta File In Python
python
fasta
updated 5 months ago by
Ram
43k • written 14.0 years ago by
Eric Normandeau
11k
62
votes
14
replies
196k
views
9 follow
How To Deal With Duplicate Row Names Error In R
r
updated 12 months ago by
zx8754
11k • written 11.2 years ago by
Diana
▴ 910
287
votes
39
replies
183k
views
29 follow
Tutorial:
How to download raw sequence data from GEO/SRA
fastq
SRA
bam
GEO
updated 13 months ago by
Ram
43k • written 9.7 years ago by
Obi Griffith
20k
131
votes
19
replies
183k
views
13 follow
What'S The Difference Between Cds And Orf?
cds
orf
updated 3.0 years ago by
kajumi
• 0 • written 11.9 years ago by
shiy05
▴ 370
151
votes
42
replies
180k
views
22 follow
How Do I Draw A Heatmap In R With Both A Color Key And Multiple Color Side Bars?
heatmap
r
updated 23 months ago by
Ram
43k • written 12.1 years ago by
Obi Griffith
20k
208
votes
21
replies
180k
views
12 follow
Forward And Reverse Strand Conventions
sequence
strand
updated 17 months ago by
Istvan Albert
100k • written 13.5 years ago by
Andrea_Bio
★ 2.8k
164
votes
8
replies
180k
views
Generate vcf.gz file and its index file vcf.gz.tbi
vcf
tabix
updated 21 months ago by
Ram
43k • written 11.4 years ago by
lyz10302012
▴ 450
124
votes
13
replies
174k
views
13 follow
How Can I Convert Bam To Sam?
bam
next-gen-sequencing
sam
updated 7 months ago by
Ram
43k • written 13.8 years ago by
Biomed
5.0k
135
votes
18
replies
170k
views
16 follow
Blast - Formatting Output
blast
format
output
updated 16 months ago by
katieostrouchov
▴ 30 • written 10.4 years ago by
timjoncooper
▴ 320
143
votes
14
replies
169k
views
16 follow
What Is The Sequencing 'Depth' ?
next-gen
sequencing
updated 5 months ago by
Ram
43k • written 14.1 years ago by
Pierre Lindenbaum
161k
98
votes
39
replies
157k
views
19 follow
Extract Reads From A Bam File That Fall Within A Given Region
bam
updated 12 months ago by
Dan
▴ 180 • written 11.8 years ago by
abi
▴ 390
231
votes
38
replies
146k
views
29 follow
Tool:
Converting Genome Coordinates From One Genome Version To Another (Ucsc Liftover, Ncbi Remap, Ensembl Api)
ensembl
genome-coordinates
liftover
updated 10 months ago by
Ram
43k • written 11.2 years ago by
Malachi Griffith
19k
362
votes
82
replies
144k
views
35 follow
Tool:
Gnu Parallel - Parallelize Serial Command Line Programs Without Changing Them
parallel
next-gen
ngs
updated 4 weeks ago by
jena
▴ 290 • written 11.2 years ago by
ole.tange
★ 4.4k
795
votes
167
replies
141k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 3 months ago by
Biostar
2.7k • written 7.4 years ago by
Istvan Albert
100k
147
votes
31
replies
137k
views
26 follow
Useful Bash Commands To Handle Fasta Files
command-line
fasta
bash
updated 9 months ago by
Ram
43k • written 12.2 years ago by
Anima Mundi
★ 2.9k
10
votes
4
replies
134k
views
How To Count The Number Of Elements In A Column Other Than 'X' In R
r
programming
updated 12.8 years ago by
Stefano Berri
4.4k • written 12.8 years ago by
Don
▴ 20
13
votes
8
replies
130k
views
7 follow
Convert A Text File Into A Fasta File
fasta
updated 19 months ago by
Ram
43k • written 12.5 years ago by
Zhshqzyc
▴ 520
68
votes
18
replies
130k
views
14 follow
from .BAM to .BAI using samtools
next-gen-sequencing
samtools
updated 3 months ago by
Pierre Lindenbaum
161k • written 9.6 years ago by
Kizuna
▴ 870
129
votes
32
replies
129k
views
29 follow
Is There A Free Alternative To Ingenuity Pathway Analysis?
network
pathway
updated 2.1 years ago by
Ram
43k • written 13.1 years ago by
Gon
▴ 540
166
votes
52
replies
128k
views
28 follow
Fastq Convert To Fasta
perl
10.5 years ago by
biolab
★ 1.4k
100
votes
9
replies
127k
views
11 follow
How To Merge Two Fastq.Gz Files?
merge
fastq
updated 2.0 years ago by
henry-keen
▴ 40 • written 10.6 years ago by
newDNASeqer
▴ 760
23
votes
9
replies
127k
views
8 follow
How to calculate "fold changes" in gene expression?
R
gene
updated 14 months ago by
Ram
43k • written 9.0 years ago by
juan.rosas1
▴ 70
26
votes
10
replies
123k
views
Calculating P-Values From Z-Scores
statistics
r
updated 22 months ago by
Ram
43k • written 12.2 years ago by
Diana
▴ 910
119
votes
25
replies
123k
views
12 follow
Where Can I Download Human Reference Genome In Fasta Format? Hgref.Fa File
human
fasta
sequence
bwa
updated 7.2 years ago by
Hajk-Georg Drost
▴ 160 • written 13.8 years ago by
Biomed
5.0k
76
votes
26
replies
120k
views
15 follow
How to count fastq reads
sequence
next-gen
fastq
reads
updated 5 months ago by
cschu181
★ 2.8k • written 9.0 years ago by
Chenglin
▴ 260
3
votes
3
replies
119k
views
Find mismatch in two columns in a data frame in R
R
updated 8.1 years ago by
dan.shea
▴ 10 • written 8.1 years ago by
dirranrak
▴ 20
120
votes
7
replies
118k
views
Bam And Indexed Bam Files
bam
index
12.3 years ago by
Sahel
▴ 260
46
votes
10
replies
115k
views
8 follow
Which Expression Units To Use, Fpkm Or Rpkm ?
fpkm
rpkm
rna-seq
updated 16 months ago by
Ram
43k • written 11.1 years ago by
biorepine
★ 1.5k
103
votes
34
replies
113k
views
23 follow
How To Split A Bam File By Chromosome
bam
updated 3 months ago by
Ram
43k • written 11.9 years ago by
GPR
▴ 390
115
votes
21
replies
113k
views
14 follow
When To Choose Python Over Perl (And Vice Versa)?
python
perl
comparison
updated 12.1 years ago by
Aditya Joshi
▴ 30 • written 12.5 years ago by
Niek De Klein
★ 2.6k
105
votes
15
replies
112k
views
16 follow
Convertion Of Gff3 To Gtf
gtf
gff
updated 5 weeks ago by
xiaoleiusc
▴ 140 • written 11.9 years ago by
GouthamAtla
12k
66
votes
9
replies
110k
views
10 follow
Blog:
Gene expression units explained: RPM, RPKM, FPKM and TPM
gene-expression
RNA-Seq
updated 14 months ago by
Ram
43k • written 6.6 years ago by
Renesh
★ 2.2k
298
votes
33
replies
108k
views
25 follow
Tutorial:
Cheat Sheet For One-Based Vs Zero-Based Coordinate Systems
coordinates
updated 14 months ago by
Ram
43k • written 10.5 years ago by
Obi Griffith
20k
91
votes
10
replies
108k
views
11 follow
Best Way To Do Pathway Analysis Of A Set Of Genes?
protein
genes
pathway
analysis
function
updated 12.0 years ago by
Guangchuang Yu
★ 2.6k • written 12.0 years ago by
Wayne
★ 1.0k
65
votes
10
replies
106k
views
6 follow
Fold change - a final explanation
RNA-Seq
updated 3.7 years ago by
naloy
▴ 10 • written 5.5 years ago by
Sebastian Hesse
▴ 340
28
votes
8
replies
106k
views
7 follow
How To Calculate Fpkm (Fragments Per Kilobase Of Exon Per Million Fragments Mapped)
rna
fpkm
rpkm
written 13.1 years ago by
User 5402
▴ 210
118
votes
15
replies
104k
views
15 follow
Economics Of A Career In Bioinformatics
career
updated 13.3 years ago by
Mona Mohamed
• 0 • written 13.5 years ago by
Terence
▴ 210
124
votes
56
replies
103k
views
24 follow
How to plot coverage and depth statistics of a bam file
bam
coverage
depth
next-gen-sequencing
updated 6 months ago by
William
▴ 30 • written 9.9 years ago by
kay
▴ 370
171
votes
41
replies
98k
views
27 follow
Multiline Fasta To Single Line Fasta
fasta
updated 7 months ago by
Ram
43k • written 12.9 years ago by
Palu
▴ 250
31
votes
8
replies
97k
views
8 follow
How Do You Start Writing An Algorithm? Help Needed!
java
structure
algorithm
updated 13.3 years ago by
Hazel
• 0 • written 14.0 years ago by
Hazel
▴ 70
85
votes
16
replies
97k
views
9 follow
Number of mapped reads from BAM file
bam
sam
reads
mapping
updated 17 months ago by
bhanu.chandra1
▴ 50 • written 9.0 years ago by
Prakki Rama
★ 2.7k
43
votes
8
replies
96k
views
samtools sorting and indexing
samtools
index
sort
updated 4.3 years ago by
onestop_data
▴ 330 • written 6.8 years ago by
ggman
▴ 80
239
votes
176
replies
95k
views
73 follow
Tutorial:
Survival analysis of TCGA patients integrating gene expression (RNASeq) data
RNA-Seq
Survival
TCGA
updated 11 months ago by
Ram
43k • written 8.7 years ago by
TriS
★ 4.7k
105
votes
31
replies
94k
views
18 follow
Best Way To Merge A Many Thousand Small Bam Files Into One Big Bam File?
bam
picard
samtools
merge
updated 4 months ago by
mmfansler
▴ 450 • written 12.8 years ago by
2184687-1231-83-
★ 5.1k
116,622 results • Page
1 of 2333
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Answer: genome assembly records not present in assembly_summary.txt
Webinar “Learning Single-cell Atlases” with Dr. Fabian Theis, author of Scanpy on May 8
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Comment: genome assembly records not present in assembly_summary.txt
Answer: genome assembly records not present in assembly_summary.txt
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Comment: PCA plot (Suggestions Needed)
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Recent Replies
Comment: Low mapping rate with Salmon
by
i.sudbery
19k
Take your STAR alignment and sum all the counts for each gene. You can either do this by providing an annotation to STAR, or by running fea…
Comment: How to convert plink files to Hapmap Format
by
bk11
★ 2.4k
If you run your data with plink 2.0, you will have ERRCODE column in your result file showing the reason behind "NA" p-value. https://www.b…
Comment: genome assembly records not present in assembly_summary.txt
by
sapuizait
▴ 10
jesus its in the Genbank file and I was looking at the refseq! I m such a moron - thanks for pointing it out -sorry about that :(
Comment: ScRNA data question
by
bk11
★ 2.4k
I wonder how the `Vlnplots` will look if you normalize the data using `NormalizeData()` function in `Seurat`. The flat line in your plot ar…
Comment: Low mapping rate with Salmon
by
GenoMax
141k
> Does that mean that the reads that are not mapped to my trascriptome are not exons/coding genes? Reads are likely aligning in regions wh…
Comment: How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
by
cmdcolin
★ 3.8k
the gencode link is broken now but here is a back up of that blogpost on archive https://web.archive.org/web/20130618221342/http://gencodeg…
Comment: Low mapping rate with Salmon
by
Patadu94
• 0
I'll try to make the decoy file again but I remember you also told me that it is only recommended and not mandatory for running `salmon`. I…
Comment: Low mapping rate with Salmon
by
Patadu94
• 0
For doing this, should I just look at the log.out.file?
Answer: Heatmap and rna-seq
by
pinheirofabiano
▴ 10
install.packages("pheatmap") library(pheatmap) setwd("/Users/data_analysis/results") data <- read.table(file…
Comment: Low mapping rate with Salmon
by
Patadu94
• 0
Thanks for the link GenoMax. But I was wondering, should not alignment and mapping have a similar rate and thus be correlated? In my case I…
Answer: Create a new bed file with all pairwise combinations between two other bed files
by
Pierre Lindenbaum
161k
bedtools intersect \ -a <(sort -t $'\t' -k1,1 -k2,2n A.bed) \ -b <(awk '{X=250000;P=int($2);printf("%s\t%d\…
Comment: Create a new bed file with all pairwise combinations between two other bed files
by
J
• 0
The command lines I wrote out for you are the ones I actually used. My original reference to bedtools intersect was because I assumed the b…
Comment: ScRNA data question
by
starswillfade
▴ 10
I just thought it was weird that considering each dot is a cell their expressions were all on a single line instead of being spread out lik…
Comment: ScRNA data question
by
starswillfade
▴ 10
![enter image description here][1] I am already using Seurat V5 I think. I am using SCTransform as it was recommended by the seurat page. I…
Answer: genome assembly records not present in assembly_summary.txt
by
GenoMax
141k
Can you clarify what `metainfo` you are referring to? The accession that you refer to does exist in [**GenBank assembly_summary file**][1…
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