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116,788 results • Page
1 of 2336
Sort: Views
Rank
Views
Votes
Replies
65
votes
3
replies
295k
views
Understanding up and down regulated genes from LOG2 foldchange or foldchange
protein
gene-expression
RNA-Seq
updated 14 months ago by
Ram
43k • written 10.0 years ago by
Whoknows
▴ 960
226
votes
39
replies
288k
views
33 follow
Gene Id Conversion Tool
david
updated 6 months ago by
Ram
43k • written 14.5 years ago by
Renee
▴ 620
279
votes
29
replies
284k
views
25 follow
How To Filter Mapped Reads With Samtools
samtools
updated 17 months ago by
Ram
43k • written 11.5 years ago by
sohadb1357
▴ 640
334
votes
92
replies
240k
views
58 follow
Tools To Calculate Average Coverage For A Bam File?
coverage
bam
sequencing
updated 9 months ago by
kalavattam
▴ 190 • written 13.3 years ago by
Biomed
5.0k
43
votes
24
replies
206k
views
25 follow
How To Update R In Ubuntu ?
r
ubuntu
updated 2.1 years ago by
matthew.pace
▴ 60 • written 10.4 years ago by
Arpitha Hebbar
▴ 210
12
votes
6
replies
201k
views
How can I uninstall my packages in Rstudio
R
Bioconductor
updated 3.7 years ago by
zx8754
11k • written 6.3 years ago by
hassanlou110
▴ 40
120
votes
31
replies
199k
views
17 follow
Correct Way To Parse A Fasta File In Python
python
fasta
updated 5 months ago by
Ram
43k • written 14.1 years ago by
Eric Normandeau
11k
62
votes
14
replies
196k
views
9 follow
How To Deal With Duplicate Row Names Error In R
r
updated 12 months ago by
zx8754
11k • written 11.3 years ago by
Diana
▴ 910
288
votes
39
replies
184k
views
29 follow
Tutorial:
How to download raw sequence data from GEO/SRA
fastq
SRA
bam
GEO
updated 13 months ago by
Ram
43k • written 9.7 years ago by
Obi Griffith
20k
131
votes
19
replies
183k
views
13 follow
What'S The Difference Between Cds And Orf?
cds
orf
updated 3.0 years ago by
kajumi
• 0 • written 11.9 years ago by
shiy05
▴ 370
164
votes
8
replies
181k
views
Generate vcf.gz file and its index file vcf.gz.tbi
vcf
tabix
updated 22 months ago by
Ram
43k • written 11.4 years ago by
lyz10302012
▴ 450
208
votes
21
replies
180k
views
12 follow
Forward And Reverse Strand Conventions
sequence
strand
updated 17 months ago by
Istvan Albert
100k • written 13.5 years ago by
Andrea_Bio
★ 2.8k
151
votes
42
replies
180k
views
22 follow
How Do I Draw A Heatmap In R With Both A Color Key And Multiple Color Side Bars?
heatmap
r
updated 23 months ago by
Ram
43k • written 12.2 years ago by
Obi Griffith
20k
124
votes
13
replies
174k
views
13 follow
How Can I Convert Bam To Sam?
bam
next-gen-sequencing
sam
updated 8 months ago by
Ram
43k • written 13.8 years ago by
Biomed
5.0k
135
votes
18
replies
170k
views
16 follow
Blast - Formatting Output
blast
format
output
updated 17 months ago by
katieostrouchov
▴ 30 • written 10.4 years ago by
timjoncooper
▴ 320
143
votes
14
replies
169k
views
16 follow
What Is The Sequencing 'Depth' ?
next-gen
sequencing
updated 5 months ago by
Ram
43k • written 14.1 years ago by
Pierre Lindenbaum
161k
98
votes
39
replies
158k
views
19 follow
Extract Reads From A Bam File That Fall Within A Given Region
bam
updated 12 months ago by
Dan
▴ 180 • written 11.8 years ago by
abi
▴ 390
231
votes
38
replies
146k
views
29 follow
Tool:
Converting Genome Coordinates From One Genome Version To Another (Ucsc Liftover, Ncbi Remap, Ensembl Api)
ensembl
genome-coordinates
liftover
updated 10 months ago by
Ram
43k • written 11.2 years ago by
Malachi Griffith
19k
362
votes
82
replies
144k
views
35 follow
Tool:
Gnu Parallel - Parallelize Serial Command Line Programs Without Changing Them
parallel
next-gen
ngs
updated 5 weeks ago by
jena
▴ 290 • written 11.2 years ago by
ole.tange
★ 4.4k
795
votes
167
replies
142k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 3 months ago by
Biostar
2.7k • written 7.4 years ago by
Istvan Albert
100k
147
votes
31
replies
138k
views
26 follow
Useful Bash Commands To Handle Fasta Files
command-line
fasta
bash
updated 9 months ago by
Ram
43k • written 12.2 years ago by
Anima Mundi
★ 2.9k
10
votes
4
replies
134k
views
How To Count The Number Of Elements In A Column Other Than 'X' In R
r
programming
updated 12.8 years ago by
Stefano Berri
4.4k • written 12.8 years ago by
Don
▴ 20
13
votes
8
replies
131k
views
7 follow
Convert A Text File Into A Fasta File
fasta
updated 19 months ago by
Ram
43k • written 12.5 years ago by
Zhshqzyc
▴ 520
68
votes
18
replies
130k
views
14 follow
from .BAM to .BAI using samtools
next-gen-sequencing
samtools
updated 3 months ago by
Pierre Lindenbaum
161k • written 9.6 years ago by
Kizuna
▴ 870
129
votes
32
replies
130k
views
29 follow
Is There A Free Alternative To Ingenuity Pathway Analysis?
network
pathway
updated 2.1 years ago by
Ram
43k • written 13.2 years ago by
Gon
▴ 540
166
votes
52
replies
129k
views
28 follow
Fastq Convert To Fasta
perl
10.5 years ago by
biolab
★ 1.4k
100
votes
9
replies
127k
views
11 follow
How To Merge Two Fastq.Gz Files?
merge
fastq
updated 2.0 years ago by
henry-keen
▴ 40 • written 10.6 years ago by
newDNASeqer
▴ 760
23
votes
9
replies
127k
views
8 follow
How to calculate "fold changes" in gene expression?
R
gene
updated 15 months ago by
Ram
43k • written 9.0 years ago by
juan.rosas1
▴ 70
26
votes
10
replies
123k
views
Calculating P-Values From Z-Scores
statistics
r
updated 22 months ago by
Ram
43k • written 12.2 years ago by
Diana
▴ 910
119
votes
25
replies
123k
views
12 follow
Where Can I Download Human Reference Genome In Fasta Format? Hgref.Fa File
human
fasta
sequence
bwa
updated 7.2 years ago by
Hajk-Georg Drost
▴ 160 • written 13.8 years ago by
Biomed
5.0k
76
votes
26
replies
120k
views
15 follow
How to count fastq reads
sequence
next-gen
fastq
reads
updated 5 months ago by
cschu181
★ 2.8k • written 9.0 years ago by
Chenglin
▴ 260
3
votes
3
replies
119k
views
Find mismatch in two columns in a data frame in R
R
updated 8.2 years ago by
dan.shea
▴ 10 • written 8.2 years ago by
dirranrak
▴ 20
121
votes
7
replies
118k
views
Bam And Indexed Bam Files
bam
index
12.4 years ago by
Sahel
▴ 260
46
votes
10
replies
115k
views
8 follow
Which Expression Units To Use, Fpkm Or Rpkm ?
fpkm
rpkm
rna-seq
updated 17 months ago by
Ram
43k • written 11.1 years ago by
biorepine
★ 1.5k
103
votes
34
replies
114k
views
23 follow
How To Split A Bam File By Chromosome
bam
updated 3 months ago by
Ram
43k • written 11.9 years ago by
GPR
▴ 390
115
votes
21
replies
113k
views
14 follow
When To Choose Python Over Perl (And Vice Versa)?
python
perl
comparison
updated 12.1 years ago by
Aditya Joshi
▴ 30 • written 12.5 years ago by
Niek De Klein
★ 2.6k
105
votes
15
replies
112k
views
16 follow
Convertion Of Gff3 To Gtf
gtf
gff
updated 6 weeks ago by
xiaoleiusc
▴ 140 • written 11.9 years ago by
GouthamAtla
12k
66
votes
9
replies
110k
views
10 follow
Blog:
Gene expression units explained: RPM, RPKM, FPKM and TPM
gene-expression
RNA-Seq
updated 15 months ago by
Ram
43k • written 6.6 years ago by
Renesh
★ 2.2k
298
votes
33
replies
109k
views
25 follow
Tutorial:
Cheat Sheet For One-Based Vs Zero-Based Coordinate Systems
coordinates
updated 14 months ago by
Ram
43k • written 10.5 years ago by
Obi Griffith
20k
66
votes
10
replies
108k
views
6 follow
Fold change - a final explanation
RNA-Seq
updated 3.7 years ago by
naloy
▴ 10 • written 5.6 years ago by
Sebastian Hesse
▴ 340
91
votes
10
replies
108k
views
11 follow
Best Way To Do Pathway Analysis Of A Set Of Genes?
protein
genes
pathway
analysis
function
updated 12.1 years ago by
Guangchuang Yu
★ 2.6k • written 12.1 years ago by
Wayne
★ 1.0k
28
votes
8
replies
106k
views
7 follow
How To Calculate Fpkm (Fragments Per Kilobase Of Exon Per Million Fragments Mapped)
rna
fpkm
rpkm
written 13.1 years ago by
User 5402
▴ 210
118
votes
15
replies
105k
views
15 follow
Economics Of A Career In Bioinformatics
career
updated 13.3 years ago by
Mona Mohamed
• 0 • written 13.5 years ago by
Terence
▴ 210
124
votes
56
replies
103k
views
24 follow
How to plot coverage and depth statistics of a bam file
bam
coverage
depth
next-gen-sequencing
updated 7 months ago by
William
▴ 30 • written 9.9 years ago by
kay
▴ 370
171
votes
41
replies
98k
views
27 follow
Multiline Fasta To Single Line Fasta
fasta
updated 7 months ago by
Ram
43k • written 12.9 years ago by
Palu
▴ 250
31
votes
8
replies
97k
views
8 follow
How Do You Start Writing An Algorithm? Help Needed!
java
structure
algorithm
updated 13.3 years ago by
Hazel
• 0 • written 14.0 years ago by
Hazel
▴ 70
85
votes
16
replies
97k
views
9 follow
Number of mapped reads from BAM file
bam
sam
reads
mapping
updated 17 months ago by
bhanu.chandra1
▴ 50 • written 9.1 years ago by
Prakki Rama
★ 2.7k
43
votes
8
replies
97k
views
samtools sorting and indexing
samtools
index
sort
updated 4.3 years ago by
onestop_data
▴ 330 • written 6.9 years ago by
ggman
▴ 90
239
votes
176
replies
95k
views
73 follow
Tutorial:
Survival analysis of TCGA patients integrating gene expression (RNASeq) data
RNA-Seq
Survival
TCGA
updated 11 months ago by
Ram
43k • written 8.8 years ago by
TriS
★ 4.7k
105
votes
31
replies
95k
views
18 follow
Best Way To Merge A Many Thousand Small Bam Files Into One Big Bam File?
bam
picard
samtools
merge
updated 4 months ago by
mmfansler
▴ 450 • written 12.8 years ago by
2184687-1231-83-
★ 5.1k
116,788 results • Page
1 of 2336
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Answer: MSA and distance matrix in R
Comment: MSA and distance matrix in R
Comment: Which hg38 file?
Answer: Which hg38 file?
Answer: Where to get the following bed file?
How to save GRanges object to csv file
Answer: How to get proteins from GFF file resulted from MAKER annotation
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Comment: HCL database download
by
sooni
▴ 20
I would like to download top markers for each subpopulation data. Is there no way? It seems different from the DGE data.
Comment: Understanding STAR output (Aligned.out.sam file)
by
GenoMax
142k
Refer to SAM alignment format: https://samtools.github.io/hts-specs/SAMv1.pdf Check section 1.4.
Answer: Seeking Guidance on Identifying Mutations in DARs from ATAC Data in Cancer Genom
by
LChart
3.9k
Step 1: Go to TCGA in the GDC and open the Cohort Builder https://portal.gdc.cancer.gov/analysis_page?app=CohortBuilder&tab=general Step …
Comment: Where to get the following bed file?
by
GenoMax
142k
SeqCap kits are discontinued. If you don't find the said file your will need to email Roche Support per this page: https://sequencing.roche…
Answer: Where to get the following bed file?
by
LChart
3.9k
UCSC maintains a list of exome probe sets and targets. You could check there: https://hgdownload.soe.ucsc.edu/gbdb/hg19/exomeProbesets/
Comment: How to compute TPM normalized values for TCGA miRNA data?
by
Ngrin
• 0
Yes, I found some similar ideas on biostar repository. So you think the authors have done something wrong? And I should only consider CPM?
Comment: Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA
by
Chris Dean
▴ 390
It is difficult to answer how they were able to identify *S. hominis* without seeing the code or paper. To address your second question, i…
Comment: Problem with Seurat package in PercentageFeatureSet function
by
bk11
★ 2.4k
Did you check where the `features.tsv.gz` file had Gene IDs or Ensembl IDs?
Answer: How to compute TPM normalized values for TCGA miRNA data?
by
dsull
★ 6.0k
TPM is irrelevant for miRNA-seq; CPM works fine. TPM tries to adjust for length effects by dividing by gene length (e.g. reducing the im…
Comment: Applying the metacell2 algorithm using python
by
Wayne
★ 2.0k
"I'm also unsure what qualifies as a lateral gene." The [README where you reference](https://metacells.readthedocs.io/en/latest/readme.htm…
Comment: Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA
by
sovrappensiero
▴ 90
Ah, yes this makes sense! I knew this was an issue with, say, using kraken2 on shotgun metagenomic reads, but I don't have a lot of experi…
Comment: How to access TCGA samples that were treated with a specific drug?
by
Qroid
▴ 40
Thank you. Maybe it's best to confirm with the authors about how they're getting responder/non-responder labels. I'll do that now.
Comment: Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA
by
Chris Dean
▴ 390
If you sequenced a short hypervariable region, most of your sequence reads will not be able to be assigned to the species level. One possi…
Comment: anRichment is missing
by
GenoMax
142k
Consider emailing Dr. Horvath about this. Until enough people make him aware of this issue WGCNA "problem" is unlikely to get fixed.
Comment: anRichment is missing
by
Tatyana
• 0
Unfortunately, there are only tutorials and codes for tutorials for WGCNA, not installation files for any their packages... :(
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