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460 results • Page
2 of 10
Sort: Views
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Views
Votes
Replies
69
votes
39
replies
4.6k
views
14 follow
Forum:
Preparation for Bioinformatics.SE closing
meta
biostars
updated 11 months ago by
Ram
43k • written 6.9 years ago by
John
13k
11
votes
13
replies
4.5k
views
6 follow
Forum:
NGS Data storage solutions for small organisations or big labs
big-data
HTS
storage
NGS
metagenomics
updated 11 months ago by
Ram
43k • written 4.9 years ago by
bioinfo
▴ 830
18
votes
7
replies
4.5k
views
Forum:
Review of the CIGAR string format
alignment
updated 11 months ago by
Ram
43k • written 5.5 years ago by
Juke34
8.6k
26
votes
10
replies
4.4k
views
6 follow
Forum:
Why not merging?
fastq
sequencing
updated 11 months ago by
Ram
43k • written 6.8 years ago by
finswimmer
16k
47
votes
30
replies
4.3k
views
10 follow
Forum:
Bioinformatics software distribution
software
updated 11 months ago by
Ram
43k • written 7.1 years ago by
Petr Ponomarenko
★ 2.8k
15
votes
6
replies
4.3k
views
Forum:
Which database software is better for bioinformatics web development? MySQL or MongoDB?
database
mysql
mongodb
software
updated 11 months ago by
Ram
43k • written 5.6 years ago by
mcclintock
▴ 10
73
votes
14
replies
4.3k
views
12 follow
Forum:
Things you wish you knew when entering the bioinformatics field
pitfalls
advice
updated 10 months ago by
Ram
43k • written 3.6 years ago by
jared.andrews07
★ 16k
10
votes
11
replies
4.2k
views
7 follow
Forum:
What made you interested in Bioinformatics?
career
updated 11 months ago by
Ram
43k • written 6.4 years ago by
Hussain Ather
▴ 990
33
votes
23
replies
4.2k
views
8 follow
Forum:
Is there a file format better suited for the era of pangenomics than the .vcf? What are its attributes?
VCF
file-format
genomics
updated 10 months ago by
Jeremy Leipzig
22k • written 10 months ago by
LauferVA
4.2k
40
votes
23
replies
4.2k
views
12 follow
Forum:
Why learn programming in bioinformatics?
Programming
updated 11 months ago by
Ram
43k • written 5.7 years ago by
joselu
▴ 110
15
votes
13
replies
4.1k
views
8 follow
Forum:
Does a PhD or work experience set you up better to do bioinformatics work in industry?
advice
industry
phd
updated 3 months ago by
benformatics
3.9k • written 17 months ago by
buhbs
▴ 30
36
votes
39
replies
4.1k
views
11 follow
Forum:
Survey/Vote: If you could double the speed of any three commandline tools, which three would they be?
blast
RNA-Seq
alignment
next-gen-sequencing
updated 11 months ago by
Ram
43k • written 6.5 years ago by
dhbradshaw
▴ 130
56
votes
19
replies
4.1k
views
9 follow
Forum:
How to apply for a job
resume
cover-letter
application
CV
updated 11 months ago by
Ram
43k • written 6.8 years ago by
Emily
23k
37
votes
16
replies
4.0k
views
9 follow
Forum:
Sanger sequencing is no longer the gold standard?
Sanger
NGS
updated 11 months ago by
Ram
43k • written 6.5 years ago by
lamteva.vera
▴ 220
6
votes
8
replies
4.0k
views
6 follow
Forum:
Workstation : More RAM or Faster RAM
RAM
Configuration
workstation
updated 11 months ago by
Ram
43k • written 5.6 years ago by
venuraherath
▴ 20
0
votes
3
replies
3.8k
views
10 follow
Forum:
Discussion question: Cutoff for SNP phylogeny
Phylogenetics
SNP
written 17 months ago by
SushiRoll
▴ 120
30
votes
13
replies
3.7k
views
11 follow
Forum:
what is your daily must see bioinformatics web site?
learn
technology
updated 10 months ago by
Ram
43k • written 9.6 years ago by
Medhat
9.7k
25
votes
11
replies
3.7k
views
6 follow
Forum:
Site Use Guidelines
meta
biostars
Guidelines
updated 8 days ago by
Ram
43k • written 14.6 years ago by
Istvan Albert
100k
19
votes
9
replies
3.7k
views
Forum:
How to self-study (get onto) Bioinformatics and Data Analysis for Genomic, for working with real life genomics data?
R
genome
RNA-Seq
Python
updated 11 months ago by
Ram
43k • written 5.8 years ago by
WUSCHEL
▴ 750
8
votes
9
replies
3.7k
views
8 follow
Forum:
How can I contribute to the Bioinformatics community as a Software Engineer
software-engineer
updated 11 months ago by
Ram
43k • written 5.5 years ago by
Chen Sun
★ 1.1k
0
votes
3
replies
3.6k
views
Forum:
How to know that our trinity denovo assembly is good or not?
Assembly
updated 11 months ago by
Ram
43k • written 5.4 years ago by
ashokkumar.mb
• 0
21
votes
12
replies
3.5k
views
Forum:
How to Use Biostars Part-3: Formatting Text and Using GitHub Gists
meta
how-to
documentation
3 months ago by
Ram
43k
4
votes
0
replies
3.5k
views
Forum:
How can unique molecular identifiers (UMIs) help to reduce quantitative biases?
NGS
Variant-Calling
Unique-Molecular-Identifier
updated 11 months ago by
Ram
43k • written 4.9 years ago by
David Langenberger
11k
3
votes
8
replies
3.5k
views
6 follow
Forum:
SSD or HHD for genome analysis
hardware
genome
sequencing
updated 11 months ago by
Ram
43k • written 4.6 years ago by
Neil
▴ 20
19
votes
6
replies
3.5k
views
Forum:
ngs vs microarray
microarray
ngs
updated 11 months ago by
Ram
43k • written 5.8 years ago by
au.rinki.bio
▴ 20
6
votes
8
replies
3.4k
views
Forum:
A hot topic in Bioinformatics 2017
paper
updated 11 months ago by
Ram
43k • written 7.1 years ago by
XBria
▴ 90
22
votes
11
replies
3.3k
views
Forum:
ExpressionSet in the age of tidyverse
expressionset
R
bioconductor
updated 11 months ago by
Ram
43k • written 6.0 years ago by
igor
13k
17
votes
14
replies
3.3k
views
Forum:
Asking for feedback on a Python library for computing alignments
alignment
Python
updated 10 months ago by
Alexander
▴ 220 • written 2.3 years ago by
bernhard.liebl
▴ 60
29
votes
18
replies
3.3k
views
7 follow
Forum:
Should I quit PhD program?
education
career
research
phd
updated 11 months ago by
Ram
43k • written 6.0 years ago by
George
▴ 30
9
votes
11
replies
3.3k
views
7 follow
Forum:
Fields with biggest demand for bioinformatics?
career
updated 11 months ago by
Ram
43k • written 5.3 years ago by
BioinformaticsLad
▴ 200
0
votes
2
replies
3.2k
views
Forum:
I’m considering a career transition from software engineering to bioinformatics. Is it a good fit for me?
career
updated 4 months ago by
GenoMax
142k • written 4 months ago by
Mango
▴ 10
13
votes
18
replies
3.2k
views
6 follow
Forum:
Bioinformatics independent study group
Proteomic
RNA-Seq
Genomic
updated 11 months ago by
Ram
43k • written 6.7 years ago by
Leite
★ 1.3k
38
votes
17
replies
3.2k
views
7 follow
Forum:
Why does bioinformatics use a special format like a BAM file instead of a database like SQLite?
sam
bam
samtools
updated 10 months ago by
Ram
43k • written 3.7 years ago by
kojix2
▴ 250
18
votes
2
replies
3.2k
views
Forum:
Scripts on the Web: Bioinformatics Recipes
bioinformatics
updated 11 months ago by
Ram
43k • written 6.1 years ago by
Istvan Albert
100k
9
votes
3
replies
3.2k
views
Forum:
Why doesn't Swift have an active bioinformatics community project like rust-bio, Biogo and BioJulia?
Swift
updated 11 months ago by
Ram
43k • written 6.3 years ago by
stevin
• 0
29
votes
10
replies
3.2k
views
Forum:
How do you perform functional testing for your Bioinformatics tools?
development
testing
bug
reliability
updated 11 months ago by
Ram
43k • written 7.1 years ago by
shenwei356
8.5k
7
votes
6
replies
3.1k
views
Forum:
transition to bioinformatics
career
wet-lab
updated 11 months ago by
Ram
43k • written 5.9 years ago by
ejms
• 0
2
votes
6
replies
3.1k
views
Forum:
RIP CentOS, CentOS 8 to be EOL'd in 2021
centos
linux-distro
os
updated 10 months ago by
Ram
43k • written 3.4 years ago by
5heikki
11k
10
votes
8
replies
3.1k
views
6 follow
Forum:
Cancer research for computer science student
cancer
research
computer-science
updated 6 months ago by
SANDHYA
• 0 • written 6.5 years ago by
mhasa006
▴ 70
64
votes
24
replies
3.0k
views
12 follow
Forum:
How much I must feel useless?
machine-learning
Programming
statistics
updated 11 months ago by
Ram
43k • written 5.6 years ago by
Molecular_genetics
▴ 80
4
votes
6
replies
3.0k
views
Forum:
Looking for advice on making a grad school decision on bioinformatics/computational biology programs
computational-biology
graduate-program
updated 11 months ago by
Ram
43k • written 7.1 years ago by
JQX
▴ 10
3
votes
4
replies
3.0k
views
6 follow
Forum:
Links for forums or discussion groups about machine learning, aging, genomics and artificial intelligence?
genome
updated 11 months ago by
Ram
43k • written 6.2 years ago by
tfhahn
▴ 50
12
votes
12
replies
3.0k
views
Forum:
Programming interview via Skype
programming-test
interview
updated 11 months ago by
Ram
43k • written 5.9 years ago by
padgaus
▴ 50
0
votes
1
reply
3.0k
views
Forum:
Bioinformatics Master thesis
thesis
updated 11 months ago by
Ram
43k • written 5.3 years ago by
lucas_
▴ 20
12
votes
2
replies
3.0k
views
Forum:
List of genome annotation tools (>140)
gene
genome
updated 11 months ago by
Ram
43k • written 4.7 years ago by
Juke34
8.6k
34
votes
22
replies
2.9k
views
11 follow
Forum:
Have we failed as bioinformatician in this time of COVID-19
Bioinformatician
COVID-19
updated 10 months ago by
Ram
43k • written 4.0 years ago by
heididunst
▴ 10
4
votes
6
replies
2.9k
views
Forum:
NGS Data Storage Best Practices (Clinical)
NGS
clinical
storage
updated 11 months ago by
Ram
43k • written 4.4 years ago by
Robert Sicko
▴ 630
45
votes
19
replies
2.9k
views
9 follow
Forum:
Where and how NGS techniques are heading for the next 5 years?
next-gen
NGS
updated 11 months ago by
Ram
43k • written 6.9 years ago by
lakhujanivijay
5.8k
3
votes
7
replies
2.9k
views
Forum:
Strategies for group submisions to SRA (BioProject)
NCBI
SRA
next-gen
Archive
Core-Facility
updated 11 months ago by
Ram
43k • written 5.5 years ago by
A. Domingues
★ 2.7k
8
votes
6
replies
2.8k
views
Forum:
Demonstrating Exponential Growth In Pre-School
education
written 10.2 years ago by
Asaf
10k
460 results • Page
2 of 10
Recent Votes
A: Decoy In Reference Assembly
A: Decoy In Reference Assembly
Decoy In Reference Assembly
Decoy In Reference Assembly
Comment: Bacterial plasmid analysis
Unsupervised clustering on gene expression data
Answer: Bacterial plasmid analysis
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Recent Replies
Comment: Problem with data downloaded from Short Reads Archive (SRA)
by
GenoMax
142k
Use `vdb-validate` included in `sratoolkit` to check your `*.sra` files for integrity.
Comment: Problem with data downloaded from Short Reads Archive (SRA)
by
ATpoint
82k
This `fastq-dump --split-files *.sra` gives you fastq files. I am not sure how, with the given information, one might debug your problem. A…
Answer: What analysis suitable to identify similarly expressed genes between two samples
by
ATpoint
82k
DESeq2 has a "lessAbs" test which gioves you a pvalue that tests whether a fold change is **below** a user-defined threshold, see https://b…
Comment: RNAseq one control two conditions, shared and exclusive genes
by
Mohamed Abderrahmane
▴ 20
In your table RawCounts, keep only the columns corresponding to condition 1 and the control. Edit the table sampleTable to align with your …
Comment: Problem with data downloaded from Short Reads Archive (SRA)
by
Begonia_pavonina
▴ 150
Thank you @atpoint, I did already investigate this error on the DADA2 github. It seems that the "BiocParallel" error can be due to multipl…
Comment: Discrepancy in read counts from fastq
by
GenoMax
142k
Try `reformat.sh` from BBMap suite and its sampling parameters. You may get a third answer. Programs do some approximations so it is probab…
Comment: Problem with data downloaded from Short Reads Archive (SRA)
by
ATpoint
82k
One cannot help you with this. The error is from R, yet you show not a single line of R code.
Comment: Bacterial plasmid analysis
by
Daria
▴ 10
I am the colleague! My tool groups together plasmids based on two genetic distances: containment (how much of the smaller plasmid's sequenc…
Comment: Subsetting and merging back Seurat Object brings different results
by
Bine
▴ 60
Ok I think I know what happened. The order of cells changed, otherwise its the same: ![image](https://github.com/satijalab/seurat/assets/7…
Answer: Bacterial plasmid analysis
by
shenwei356
8.5k
My colleague has an unpublished tool for plasmid analysis (clustering), and it has really good results. You might have a try. https://githu…
Comment: Odd alignment question/finding
by
ATpoint
82k
bwa mem is not fully deterministic, see from the developer: https://www.biostars.org/p/238628/#238817
Comment: How to perform single cell multimodal integration using Seurat for Geo datasets
by
ATpoint
82k
It's funny that you ask because the paper that these data were used for is called `Integrated analysis of multimodal single-cell data` from…
Comment: Odd alignment question/finding
by
liorglic
★ 1.4k
I can't come up with an explanation, but two things you can try for debugging are: 1) look at the fastp stats - do they add up and make sen…
Answer: Bacterial plasmid analysis
by
Mensur Dlakic
★ 27k
If you put all your sequences in separate files but in the same directory, this program will build a cladogram based on average nucleotide …
Comment: POSSUM not working due to incompatible pssm file
by
rianna.collins
• 0
Thank you so much. I'm new to this and didn't realise there was a code option.
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