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116,638 results • Page
3 of 2333
Sort: replies
Rank
Views
Votes
Replies
57
votes
43
replies
16k
views
8 follow
Forum:
Best RNA-Seq aligner: A comparison of mapping tools
NGS
alignment
genome
RNA-Seq
sequence
updated 13 months ago by
Ram
43k • written 5.3 years ago by
David Langenberger
11k
19
votes
43
replies
5.6k
views
Annotation of huge number of CNV files
CNV annotation TCGA
5.7 years ago by
nazaninhoseinkhan
▴ 520
138
votes
42
replies
22k
views
22 follow
Forum:
Are We Rude/Do We Expect Too Much From People Asking Questions On This Forum?
meta
updated 13 months ago by
LauferVA
4.2k • written 10.9 years ago by
Whetting
★ 1.6k
11
votes
42
replies
4.1k
views
over presented kmer in fastq
fastqc
RNA-Seq
6.6 years ago by
Sam
▴ 150
8
votes
42
replies
4.7k
views
NaS (Nanopore Synthetic-long) help
Assembly
preprocessing
updated 16 months ago by
Ram
43k • written 8.9 years ago by
midox
▴ 290
57
votes
42
replies
6.6k
views
12 follow
Forum:
Why is academic software hard to install?
software-installation
updated 2.3 years ago by
Ram
43k • written 9.5 years ago by
lh3
33k
103
votes
42
replies
31k
views
34 follow
Forum:
List of cloud genomics companies
cloud-genomics
updated 20 days ago by
Jeremy Leipzig
22k • written 10.5 years ago by
14134125465346445
★ 3.6k
151
votes
42
replies
180k
views
22 follow
How Do I Draw A Heatmap In R With Both A Color Key And Multiple Color Side Bars?
heatmap
r
updated 23 months ago by
Ram
43k • written 12.2 years ago by
Obi Griffith
20k
28
votes
42
replies
7.1k
views
7 follow
How to add tophat and bowtie to the path?
export PATH
bowtie2.2.9
tophat2
7.4 years ago by
mirza
▴ 180
23
votes
41
replies
4.3k
views
6 follow
I want to correct the erroneous barcode file, and the Python code that I've written, using Biopython, is very slow. How can I make this process fast…
NGS
biopython
illumina
WGS
updated 11 months ago by
Ram
43k • written 12 months ago by
Vijith
▴ 30
98
votes
41
replies
6.2k
views
17 follow
Forum:
I am really pissed off by the bioinformatics software world. Do/can we have a better solution?
software-error
next-gen-sequencing
updated 12 months ago by
Ram
43k • written 7.7 years ago by
moxu
▴ 510
203
votes
41
replies
25k
views
20 follow
Forum:
Genomics is not Special. Computational Biologists are reinventing the wheel for big data biology analysis
genomics
cram
gatk
galaxy
updated 14 months ago by
Ram
43k • written 9.5 years ago by
William
★ 5.3k
26
votes
41
replies
17k
views
10 follow
WGCNA modules and categorical traits relationship
WGCNA
updated 17 months ago by
lovelymaoqin
• 0 • written 6.3 years ago by
BrunoGiotti
▴ 120
19
votes
41
replies
9.1k
views
7 follow
n.sv number for batch effects in RNA-seq
RNA-Seq
updated 6.7 years ago by
ivivek_ngs
★ 5.2k • written 6.7 years ago by
sophialovechan
▴ 80
38
votes
41
replies
59k
views
9 follow
Volcano Plot from DEseq2
R
written 6.5 years ago by
1769mkc
★ 1.2k
5
votes
41
replies
2.9k
views
Insert sequence in nt database
nt
3.9 years ago by
anasofiamoreira94
▴ 80
18
votes
41
replies
14k
views
6 follow
paired end illumina reads
Assembly
paired end
updated 21 months ago by
Ram
43k • written 8.5 years ago by
midox
▴ 290
13
votes
41
replies
2.4k
views
Is my file created completely
sam
BWA-MEM
BWA
updated 13 months ago by
Ram
43k • written 7.0 years ago by
micro32uvas
▴ 10
18
votes
41
replies
3.4k
views
6 follow
Convert amino acid sequences into nucleotide sequences
nucleotide
protein
updated 4 months ago by
Joe
21k • written 6 months ago by
sil_bioinfo
▴ 40
171
votes
41
replies
98k
views
27 follow
Multiline Fasta To Single Line Fasta
fasta
updated 7 months ago by
Ram
43k • written 12.9 years ago by
Palu
▴ 250
16
votes
41
replies
9.6k
views
How to extract information from headers of fasta file
sequence
updated 22 months ago by
Ram
43k • written 9.1 years ago by
Crystal
▴ 70
31
votes
41
replies
2.7k
views
12 follow
Forum:
Question regarding journal publications
publications
journals
updated 10 months ago by
Ram
43k • written 4.2 years ago by
K.Gee
▴ 40
18
votes
40
replies
18k
views
16 follow
Tutorial:
Easy way to run easily orthoMCL (Copy & paste)
all-v-all
orthomcl
updated 3.8 years ago by
krishdb38
• 0 • written 7.8 years ago by
Esaie
▴ 170
40
votes
40
replies
11k
views
9 follow
What is the reason for trimming reads to 30 bp for ATAC-seq aligning?
ATAC-seq
alignment
updated 6.2 years ago by
Kevin Blighe
87k • written 7.6 years ago by
datascientist28
▴ 560
67
votes
40
replies
9.7k
views
12 follow
Forum:
I want to re-open the old debate: python or perl ?
perl
python
updated 2.2 years ago by
Ram
43k • written 9.4 years ago by
Gabriel R.
★ 2.9k
10
votes
40
replies
6.5k
views
weird insert size post trimming
RNA-Seq
updated 6.7 years ago by
Gabriel R.
★ 2.9k • written 6.7 years ago by
badribio
▴ 290
16
votes
40
replies
6.3k
views
About TCGA CNV data preprocessing
SNP
CNV
updated 5.5 years ago by
Kevin Blighe
87k • written 5.5 years ago by
Eric Wang
▴ 50
68
votes
40
replies
6.4k
views
11 follow
Can We Agree On A Short Twitter Hashtag For Nextgen Sequencing?
next-gen
sequencing
sequencing
updated 12.5 years ago by
pmenzel
▴ 310 • written 12.5 years ago by
Samuel Lampa
★ 1.3k
6
votes
40
replies
11k
views
Fastx_Collapser Has No Fastq Output
fastx
RNA-seq
updated 14 months ago by
Ram
43k • written 10.6 years ago by
nbvasani
▴ 240
151
votes
39
replies
25k
views
20 follow
Forum:
A Farewell To Bioinformatics
bioinformatics
updated 14 months ago by
Ram
43k • written 11.3 years ago by
Martin A Hansen
3.0k
20
votes
39
replies
9.8k
views
7 follow
Is Blast+ Running As Fast As It Could ?
blast
blast
memory
updated 8.6 years ago by
Kumar
▴ 170 • written 12.3 years ago by
Amr
▴ 160
36
votes
39
replies
4.1k
views
11 follow
Forum:
Survey/Vote: If you could double the speed of any three commandline tools, which three would they be?
blast
RNA-Seq
alignment
next-gen-sequencing
updated 11 months ago by
Ram
43k • written 6.5 years ago by
dhbradshaw
▴ 130
55
votes
39
replies
5.8k
views
8 follow
Programming Challenge - Synthetic Whole Genome Vcf
vcf
python
perl
awk
updated 11.6 years ago by
Rm
8.3k • written 11.6 years ago by
Mahdi Sarmady
▴ 310
66
votes
39
replies
7.5k
views
13 follow
What Do You Consider The Most Trivial And The Most Challenging Tasks In Your Particular Field Of Work?
career
subjective
updated 3.1 years ago by
Ram
43k • written 14.1 years ago by
Marcos De Carvalho
▴ 310
11
votes
39
replies
4.2k
views
Any advice for a de novo genome assembly
k-mer
de novo
genome
assembly
updated 7.4 years ago by
krsahlin
▴ 60 • written 7.4 years ago by
Picasa
▴ 640
98
votes
39
replies
157k
views
19 follow
Extract Reads From A Bam File That Fall Within A Given Region
bam
updated 12 months ago by
Dan
▴ 180 • written 11.8 years ago by
abi
▴ 390
25
votes
39
replies
5.8k
views
8 follow
Forum:
Bioinformatics Study Group
study-group
updated 14 months ago by
Ram
43k • written 10.8 years ago by
Olivier
▴ 440
81
votes
39
replies
7.6k
views
11 follow
News:
State Of Biostar - Future Directions (January 2013)
biostars
updated 14 months ago by
Ram
43k • written 11.3 years ago by
Istvan Albert
100k
287
votes
39
replies
183k
views
29 follow
Tutorial:
How to download raw sequence data from GEO/SRA
fastq
SRA
bam
GEO
updated 13 months ago by
Ram
43k • written 9.7 years ago by
Obi Griffith
20k
18
votes
39
replies
4.3k
views
Tool:
CBioInfCpp.h as a C++ lib containing some functions for bioinformatics
cpp
updated 11 months ago by
Ram
43k • written 5.0 years ago by
chernouhov sergey
▴ 50
69
votes
39
replies
4.6k
views
14 follow
Forum:
Preparation for Bioinformatics.SE closing
meta
biostars
updated 11 months ago by
Ram
43k • written 6.9 years ago by
John
13k
226
votes
39
replies
288k
views
33 follow
Gene Id Conversion Tool
david
updated 6 months ago by
Ram
43k • written 14.5 years ago by
Renee
▴ 620
39
votes
38
replies
9.0k
views
9 follow
DNA composition - all k-mers and their frequency in some sequencing data
sequencing
updated 20 months ago by
Ram
43k • written 8.5 years ago by
John
13k
78
votes
38
replies
33k
views
15 follow
Illumina Instrument Type from fastq?
fastq
updated 8 weeks ago by
nickp60
▴ 60 • written 7.9 years ago by
andrew.j.skelton73
6.5k
129
votes
38
replies
8.2k
views
21 follow
Forum:
Who are the Greatest Bioinformaticians Of All Time (GBOAT)
bioinformatics
updated 11 months ago by
Ram
43k • written 5.1 years ago by
Istvan Albert
100k
48
votes
38
replies
17k
views
8 follow
Forum:
Eukaryotic Genome Annotation in 2016
software
genome
annotation
updated 12 months ago by
Ram
43k • written 8.1 years ago by
Eric Normandeau
11k
231
votes
38
replies
146k
views
29 follow
Tool:
Converting Genome Coordinates From One Genome Version To Another (Ucsc Liftover, Ncbi Remap, Ensembl Api)
ensembl
genome-coordinates
liftover
updated 10 months ago by
Ram
43k • written 11.2 years ago by
Malachi Griffith
19k
16
votes
38
replies
17k
views
Differential gene Analysis by Limma
Limma
Microarray
R
updated 2.0 years ago by
Ram
43k • written 9.2 years ago by
Mo
▴ 920
53
votes
38
replies
4.5k
views
10 follow
Multiple alignment software
alignment
clustal omega
tcoffee
updated 4.6 years ago by
Istvan Albert
100k • written 4.6 years ago by
juanjo75es
▴ 130
27
votes
38
replies
20k
views
Heatmap based with FPKM values
RNA-Seq
next-gen
R
updated 5.0 years ago by
Biostar
20 • written 6.4 years ago by
Mehmet
▴ 820
116,638 results • Page
3 of 2333
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Comment: NGS forensics: how to know if data is fabricated
Answer: How to use limma to find differentially expressed genes in response to a continu
Batch effects : ComBat or removebatcheffects (limma package) ?
How to input margin to UpsetR figure
Comment: How to assign cell types after integration in scRNA
NGS forensics: how to know if data is fabricated
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Recent Replies
Comment: How many reads for WGS Sequencing?
by
Mensur Dlakic
★ 27k
I downloaded the data in two files from the link you provided. With this command: megahit -1 ERR072246_1.fastq.gz -2 ERR072246_2.fastq…
Comment: How many reads for WGS Sequencing?
by
Mensur Dlakic
★ 27k
If you have Linux the assembly should work on your system, but 8 GB is generally not enough for assembling larger genomes.
Comment: How many reads for WGS Sequencing?
by
Mensur Dlakic
★ 27k
I think you might be getting stuck on less relevant parts of my exercise. The most important point was that nothing is wrong with the data.…
Comment: What does it mean single base resolution in sequencing?
by
dsull
★ 5.9k
Great comprehensive explanation and overview of the technologies -- with regards to sequencing assays, the one thing I'd add is SNV identif…
Answer: What does it mean single base resolution in sequencing?
by
dsull
★ 5.9k
I think that term means slightly different things in different contexts. For example, it's useful to profile mRNA modifications (e.g. m6a) …
Comment: Cannot process all the reads in a fast5 file?
by
chujie
• 0
Hi, I met the same problem, have you found the solution?
Answer: What does it mean single base resolution in sequencing?
by
LauferVA
4.2k
Hi @fe3e6f65 , In a single sentence, the answer to your question can be summarized as, "*the resolution of a genomic technology refers to …
Comment: PCA plot
by
LauferVA
4.2k
Without more context, we cannot interpret this PCA plot. There are many possibilities. In addition, this question is basic enough that it w…
Comment: NGS forensics: how to know if data is fabricated
by
Jeremy Leipzig
22k
i have the name ready: out**liar**
Comment: What does it mean single base resolution in sequencing?
by
GenoMax
141k
Sequencing by hybridization (https://en.wikipedia.org/wiki/Sequencing_by_hybridization ) is likely not giving you single base resolution. B…
Comment: Fetch table from clinvar database according to a list of rsid
by
ashaneev07
▴ 20
Hii... i have updated the script in python..But, still getting no data found. Actually the data is there, i have print the parsed html file…
Comment: How to assign cell types after integration in scRNA
by
ATpoint
82k
https://bioconductor.org/books/release/OSCA.multisample/using-corrected-values.html
Comment: Downloading full alignments from Pfam
by
GenoMax
141k
Not as convenient but the entire set of full alignments can be downloaded here: https://ftp.ebi.ac.uk/pub/databases/Pfam/current_release/Pf…
Comment: How many reads for WGS Sequencing?
by
GenoMax
141k
> What does clipped Fastq mean? Probably means that NCBI has already scanned and trimmed adapter sequencers. > both forward and revers…
Comment: How many reads for WGS Sequencing?
by
GenoMax
141k
You can use the default `adapters.fa` file included in the `resources` folder of [**BBMap suite**][1] (program to use is `bbduk.sh`) or a p…
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